• Archives of Pharmacal Research
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• 제21권3호
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• pp.291-297
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• 1998

Cop protein has been overexpressed in Escherichia coli using a T7 RNA polymerase system. Purification to apparent homogeneity was achieved by the sequential chromatography on ion exchange, affinity chromatography, and reverse phase high performance liquid chromatography system. The molecular weight of the purified Cop was estimated as 6.1 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). But the molecular mass of the native state Cop was shown to be 19 kDa by an analytical high performance size exclusion chromatography, suggesting a trimer-like structure in 50 mM Tris-HCI buffer (pH 7.5) containing 100 mM NaCl. Cop protein Was calculated to contain $39.1% {\alpha}-helix, 16.8% {\beta}-sheet$, 17.4% turn, and 26.8% random structure. The DNA binding property of Cop protein expressed in E. coli Was preserved during the expression and purification process. The isoelectric point of Cop was determined to be 9.0. The results of amino acid composition analysis and N-terminal amino acid sequencing of Cop showed that it has the same amino acid composition and N-terminal amino acid sequence as those deduced from its DNA sequence analysis, except for the partial removal of N-terminal methionine residue by methionyl-aminopeptidase in E. coli.

• 한국응용과학기술학회지
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• 제35권3호
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• pp.948-955
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• 2018

본 연구의 목적은 평발을 가진 여성 노인의 계단 하강 보행 시 일반인솔 및 아치 지지형 인솔을 적용에 따른 족저압력 및 압력중심점 변인들의 차이를 조사하는데 목적이 있다. 족저압력 분석장비(Pedar-X, Novel, Germany)를 사용하여 14명의 평발 노인을 대상으로 3종류(일반인솔, A형 인솔, B형 인솔)의 인솔착용 후 최대족저압력, 평균족저압력, 접촉면적, 압력중심점의 이동거리, 변위 및 최대범위의 평균값을 산출하였으며, 일원변량분석(one-way ANOVA)를 이용하여 인솔 종류간 평균을 비교하였다. 족저 압력 변인 중 최대족저압력은 중족부의 M3, 평균족저압력은 M2, M3, M4 영역에서, 접촉면적은 M2, M3, 그리고 M6 영역에서 통계적으로 유의한 차이를 보였으며, 압력중심점 변인 중 전후축과 좌우축에서 압력중심점의 이동거리에서 통계적으로 유의한 차이를 보였다. 족저압력 평가결과 아치 지지 기능을 가진 인솔을 삽입한 A와 B형 인솔에서 족궁지지 영역인 M3의 최대압력은 B형 인솔과 A형 인솔이 일반인솔과 비교했을 때 높게 나타났다. 좌우축, 전후축 압력중심점의 이동거리는 A형과 B형 인솔 모두 일반 인솔에 비해 짧게 나타났다.

• BMB Reports
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• 제45권2호
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• pp.85-90
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• 2012

Our previous study demonstrated that CopA3, a disulfide dimer of the coprisin peptide analogue (LLCIALRKK), has antibacterial activity. In this study, we assessed whether CopA3 caused cellular toxicity in various mammalian cell lines. CopA3 selectively caused a marked decrease in cell viability in Jurkat T, U937, and AML-2 cells (human leukemia cells), but was not cytotoxic to Caki or Hela cells. Fragmentation of DNA, a marker of apoptosis, was also confirmed in the leukemia cell lines, but not in the other cells. CopA3-induced apoptosis in leukemia cells was mediated by apoptosis inducing factor (AIF), indicating induction of a caspase-independent signaling pathway.

• 대한수학회보
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• 제55권6호
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• pp.1667-1690
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• 2018

We consider the minimum order of a graph G with a given lazy cop number $c_L(G)$. Sullivan, Townsend and Werzanski [7] showed that the minimum order of a connected graph with lazy cop number 3 is 9 and $k_3{\square}k_3$ is the unique graph on nine vertices which requires three lazy cops. They conjectured that for a graph G on n vertices with ${\Delta}(G){\geq}n-k^2$, $c_L(G){\leq}k$. We proved that the conjecture is true for k = 4. Furthermore, we showed that the Petersen graph is the unique connected graph G on 10 vertices with ${\Delta}(G){\leq}3$ having lazy cop number 3 and the minimum order of a connected graph with lazy cop number 4 is 16.

• 한국운동역학회지
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• 제20권4호
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• pp.479-486
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• 2010

The purpose of this study was to determine the effects of high-heeled shoe on the quiet standing and gait balance. Twenty women (mean height: $161.6{\pm}3.3\;cm$, mean body mass: $53.8{\pm}6.3\;kg$, mean age: $23.8{\pm}2.7$ yrs..) who were without history or complain of lower limb pain took part in this study. They were asked to stand quietly on a force platform for 30 sec and walk on it at their preferred walking speed (mean speed $3.14{\pm}0.5\;km/hr$.) with wearing three different high-heeled shoe, 3, 7, 9 cm high for collecting data. Data were randomly recorded to collect two trials for quiet standing and five trials for walking The parameters to have been analyzed for comparison between three conditions of the height of high-heeled shoe were COP(Center of Pressure) range, COP velocity, sway area, and free moment on the static balance and COP range, COP velocity, and free moment on the dynamic balance. In this study, high-heel height affected on the COP range and velocity in the ante-posterior direction during walking, dynamic balance, but didn't affect on the quiet standing, static balance.

• 한국균학회지
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• 제35권1호
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• pp.1-10
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• 2007

세포벽은 진균류의 생존과 삼투안전성 유지에 필수적인 구조체로, 세포부착성 단백질이나 가수분해효소 등과 같은 생물학적 활성을 갖는 다양한 단백질이 결합하거나 그 속에 자리잡고 작용할 수 있게 한다. 최근 효모류인 Saccharomyces cerevisiae와 사상균인 Aspergillus nidulans에서 세포 내 단백질 분비 소낭의 하나인 COPI 소낭을 구성하는 ${\alpha}-COP$ 단백질에 돌연변이가 일어날 경우, 온도의존적 삼투감수성이 나타나는 것으로 밝혀졌다. 이러한 사실은 ${\alpha}-COP$이 베타-1,3-글루칸 합성효소 복합체를 구성하는 단백질과 세포벽 단백질의 이송과정에 중요한 역할을 함으로써 세포벽 안정성 유지에 기여함을 시사하는 것이다. 본 총설에서는 세포 내 단백질 이송기구 중에서도 COPI 소낭을 구성하는 ${\alpha}-COP$과 균류의 세포벽 형성과정과의 관계에 대하여 기술하는 한편, 단백질 분비기구에 결손이 생긴 돌연변 이주를 이용한 세포벽 합성기작에 대한 기초 및 응용연구의 가능성에 대하여 검토하여 보았다.

• 한국운동역학회지
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• 제22권3호
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• pp.261-268
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• 2012

The purpose of this study was to qualitatively analyze pressure intensity and the center of pressure(COP) trajectory according to shoe type. Subjects were ten first-year female university students. The EMED-AT 25/D(Novel, Germany) was used to measure pressure intensity and COP trajectory. The COP Excursion Index(CPEI) was used for within subject test design. Independent variables were bare feet and six types of shoes. Dependent variables were center of pressure trajectory and pressure intensity. Barefeet and five toed shoes had a similar pressure intensity and COP trajectory. COP trajectory for all other shoe types showed a medial wobble at the heel. Pressure intensity for all other shoe types was related to the structure of the shoes. In conclusion, different shoe types can not only affect gait, but they can also influence foot deformities, pain, and dysfunction.

• 한국운동역학회지
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• 제32권3호
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• pp.80-86
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• 2022

Objective: The purpose of this study was to investigate the effects of three rotational jump conditions (standing jump, left rotational jump and right rotational jump) on stability through center of pressure (COP) and EMG variables analysis. Method: A total of 16 college students (age: 24.13 ± 7.17 years, height: 169.24 ± 8.23 cm, weight: 65.65 ± 13.88 kg) participated in this study. The study used wireless two COP plates and wireless eight channel EMG. The analyized variables were 11 variables for COP and RMS for EMG, which were analyzed using one-way analysis of variance with repeated measures according to three rotational jump conditions. Results: Among the COP variables, left rotational jump (LRJ) and right rotational jump (RRJ) were larger than standing jump (SJ) for left and right amplitude, area, total displacement, and average velocity for both feet among the variables of COP, and for area of the left foot, RRJ was larger than that of SJ. Among the EMG variables, there was no statistical difference between the muscle activations, but the muscle activity was significantly higher in the order of RRJ, LRJ, and SJ according to direction of rotation. Conclusion: Although the results of COP and EMG were not consistent through this study, it can be expected that the differences in COP was due to the amount of rotation during rotational jump-landing in the left and right directions, and that the EMG is determined by the lateral movements required for rotation.

• 대한인간공학회지
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• 제36권5호
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• pp.395-409
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• 2017

Objective: The purpose of this study was to optimize the number and positions of foot pressure sensors using the reliability analysis of the center of pressure (COP) in smart shoes. Background: Foot pressure can be different according to foot region, and it is important which region of the foot pressure needs to be measured. Method: Thirty adults (age: $20.5{\pm}1.8years$, body weight: $71.4{\pm}6.5kg$, height: $1.76{\pm}0.04m$) participated in this study. The foot pressure data were collected using the insole of Pedar-X system (Novel GmbH, USA) with a sampling frequency of 100Hz during 1.3m/s speed walking on the treadmill (Instrumented treadmill, Bertec, USA). The intraclass correlation coefficients (ICC) were calculated between the COP positions using 4, 5, 6, 7, 8, and 99 sensors, while one-way repeated measure ANOVA was performed between the standard deviation (SD) of the COP positions. Results: The medio-lateral (M/L) COP position using 99 sensors was positively correlated with the M/L COP positions using 6, 7, and 8 sensors; however, it was not correlated with the M/L COP positions using 4 and 5 sensors during landing phase (1~4%) (p<.05). The antero-posterior (A/P) COP position using 99 sensors was positively correlated with the A/P COP positions using 4, 5, 6, 7, and 8 sensors (p<.05). The SD of the COP position using 99 sensors was smaller than the SD of the M/L COP positions using 4, 5, 6, 7, and 8 sensors (p<.05). Conclusion: Based on our findings, it is desirable to arrange at least 6 sensors in smart shoes. Application: The study of optimizing the number and positions of foot pressure sensors would contribute to developing more effective smart shoes using foot pressure technology.

• The Journal of Korean Physical Therapy
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• 제35권5호
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• pp.156-161
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• 2023

Purpose: This study examined the effects of subjective visual vertical perception and head orientation on static balance control. Methods: The subjects were 25 young and healthy adults. The vertical perception was measured using a subjective visual vertical (SVV), and the Center of pressure (COP) parameter was analyzed by continuously measuring the movement of the COP to determine the changes in static postural control. The group was divided based on a deviation of 3° in SVV (11 of SVV≥3°, 14 of SVV<3°) and measured with different head orientations: front, up, down, left, and right in the upright and tandem positions, respectively. Results: In the upright position, the SVV≥3° group had significantly larger values for all COP parameters (Sway length, Surface, Delta X, Delta Y, and Average speed) compared to the SVV<3° group (p<0.05). In the tandem stance, only the Ellipse Surface value was significantly larger among the COP parameters in the group with SVV≥3° compared to the group with SVV<3°(p<0.05). In contrast, the other COP parameters were not significantly different (p>0.05). The effects of static balance control on the head orientation were not statistically significant (p>0.05), and the interactions between the subjective vertical perception and head orientation were not significant (p>0.05). Conclusion: These results suggest that pathological deviations in SVV are associated with impaired static balance performance. This study can provide a therapeutic rationale for using visuospatial cognitive feedback training to improve the static balance.