• 제목/요약/키워드: Conventional Methods

검색결과 9,189건 처리시간 0.036초

Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction

  • Ahn, Jong Gyun;Choi, Seong Yeol;Kim, Dong Soo;Kim, Ki Hwan
    • Clinical and Experimental Pediatrics
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    • 제55권11호
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    • pp.424-429
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    • 2012
  • Purpose: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneumoniae -positive results. Methods: Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Children's Hospital in Korea between March 2009 and June 2010. For identification and serotype determination of pneumococci from the NP secretions, the secretions were evaluated via multiplex PCR technique with 35 serotype-specific primers arranged in 8 multiplex PCR sets and conventional bacteriological culture technique. Results: Among the results for 793 samples that underwent both bacterial culture and PCR analysis for pneumococcal detection, 153 (19.3%) results obtained by PCR and 81 (10.2%) results obtained by conventional culture technique were positive for S. pneumoniae. The predominant serotypes observed, in order of decreasing frequency, were 19A (23%), 6A/B (16%), 19F (11%), 15B/C (5%), 15A (5%), and 11A (4%); further, 26% of the isolates were non-typeable. Conclusion: As opposed to conventional bacteriological tests, PCR analysis can accurately and rapidly identify pneumococcal serotypes.

새로운 기관튜브 고정기구 적용이 비계획적 발관과 구강·안면 피부 통합성에 미치는 효과 (Effects on Unplanned Extubation, Oral Mucosa, and Facial Skin Integrity of New Method to Secure Endotracheal Tube)

  • 김정숙;이은숙;박진향
    • 임상간호연구
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    • 제15권3호
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    • pp.49-59
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    • 2009
  • Purpose: Examination was done of the effects on unplanned extubation, oral mucosa, and facial skin integrity in patients with oral intubation secured with a new method using Multifix Endo II. Methods: A prospective quasai-experimental design was used. Data were collected during a 3-week EICU admission period between Jun. 1 and Nov. 30. Two hundreds seventeen (conventional taping group: 105, Multifix Endo II group: 112) orally intubated adults were enrolled in the study at the EICU of the C University Hospital. Results: The incidence of unplanned extubation (${\chi}^2=11.580$, p=0.03) and scores for the facial skin integrity impairment of the Mutifix Endo II group (t=5.28, p=.000) were significantly lower than those of the conventional taping group even though the scores for oral mucosa impairment were not different. The nurse's evaluation of the clinical effectiveness of the two methods including convenience, comfort, safety, and satisfaction were also higher for the new securement method compared to the conventional taping group. Conclusion: The securement method using Mutifix Endo II was more effective than the conventional method in the prevention of unplanned extubation and maintenance facial skin integrity.

10 MV 이상 고에너지 치료 시 발생되는 광중성자의 영향 (The Effect of Photoneutron Dose in High Energy Radiotherapy)

  • 박병석;안종호;권동열;서정민;송기원
    • 대한방사선치료학회지
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    • 제25권1호
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    • pp.9-14
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    • 2013
  • 목 적: 10 MV 이상 고에너지 방사선 치료 시 광핵반응을 통해 광중성자가 발생된다. 광중성자는 방사선하중계수가 X선 보다 커 작은 선량에도 인체에 미치는 영향이 크므로 정확한 선량계산 및 고려가 필요하다. 이에 광자선 에너지 크기와 치료방법에 따른 광중성자의 선량변화를 공간적인 측면에서 비교 분석하였다. 대상 및 방법: 광자선의 에너지 크기에 따른 광중성자의 선량 변화를 측정하고자 동일한 치료부위의 환자를 대상으로 치료 계획을 10 MV와 15 MV conventional plan으로 각각 만들었다. 그리고 치료방법에 따른 차이에 대해 측정하고자 10 MV conventional plan과 10 MV IMRT plan을 각각 만들었다. 검출기의 위치는 광자선원으로부터 isocenter까지의 거리를 100 cm으로 정한 기준점을 $^3He$비례계수기의 중심점에 위치시키고 광중성자 선량을 측정하였다. 또한 중심점을 기준으로 couch의 longitudinal 방향으로 상방, 하방 50 cm 방향으로 각각 $^3He$비례계수기를 위치시켜 위치변화에 따른 선량변화를 측정하여 상용프로그램을 이용하여 분석하였다. 결 과: 에너지 크기에 따른 광중성자의 평균 누적선량은 10 MV, 15 MV conventional RT시 각각 $220.27{\mu}Sv$, $526.61{\mu}Sv$로 15 MV conventional RT가 평균 2.39배의 선량이 증가하였다. 동일한 에너지의 conventional RT와 IMRT의 광중성자의 평균 누적선량은 Conventional RT, IMRT 시 각각 $220.27{\mu}Sv$, $308.27{\mu}Sv$로 IMRT가 1.40배의 선량이 증가하였다. 측정위치에 따른 광중성자의 누적선량은 Conventional RT시 point 2가 3보다 10 MV에서는 약 7.1%, 15 MV에서는 3.0%로 유의하게 높게 측정되었다. 결 론: 고에너지 방사선 치료 시 광중성자에 의한 불필요한 선량을 줄이기 위해 에너지의 선택, 치료 방법의 결정 및 환자의 position을 고려해야 할 것이다. 또한 광중성자의 선량 데이터를 체계화하여 전산화계획 프로그램에 적용되는 방안을 모색하여야 할 것이다. 이는 환자에게 불필요한 선량을 최소화하고 방사선 치료로 인한 2차 암 발생확률과 부작용을 감소시킬 수 있을 것으로 사료된다.

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보완대체의학의 정의 변화와 국내 관련 연구 분석 (Changing Definitions of Complementary and Alternative Medicine and Related Research Analysis in Korea)

  • 이태형;김남일;맹웅재
    • 한국한의학연구원논문집
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    • 제16권2호
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    • pp.43-55
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    • 2010
  • Objective : There is a growing interest on Complementary and Alternative Medicine(CAM). But the term 'CAM' often causes confusion, for the perception of CAM is determined by how the relationship between CAM and Conventional Medicine is established. Methods : In this paper, we researched the concept of CAM through the change in the terminology and definition of CAM throughout history. Furthermore, we also analyzed related papers in Korea. Results : International studies show that the relationship between Conventional Medicine and CAM has become more cooperative. Meanwhile, in Korea, Korean Medicine is categorized as a type of Conventional Medicine, not CAM. Conclusion : Though Korean Medicine is included in Conventional Medicine in Korea, the research methodology is still restricted in ways of bio-medical science. Further study on the original research method of Korean Medicine is needed.

Bulk건조에서 주맥건조방법이 잎담배 이화학성에 미치는 영향 (Effect of Stem Drying Method during Curing Process on Physico-chemical Properties in Bulk Cured Leaves)

  • 이철환;이병철;진정의
    • 한국연초학회지
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    • 제22권2호
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    • pp.133-137
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    • 2000
  • The bulk curing experiment to improve the quality of flue-cured leaves were carried out to evaluate relationship between the modified(3 step-up) drying and conventional drying method in bulk curing process. Modified drying method was somewhat higher values in yellowing color index of cured leaves, and less brittle than those in conventional drying program. As to the chemical properties, there was no difference in chemical component levels in cured leaves between the modified and the conventional methods, while the major chemical compounds in relation to aromatic essential oil of cured leaves showed mostly higher level in the modified method than that in conventional drying method. Additionally 3 step-up drying method increased the tobacco quality by 2 % in price per kg compared with conventional drying method.

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흉, 복부 전산화 단층촬영이 정상인 외상 환자에게 척추 단순촬영이 필요한가? (Is There a Need for Conventional Spine Radiographs Following a Negative Chest and Abdominal CT in Trauma Patients?)

  • 오성찬
    • Journal of Trauma and Injury
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    • 제22권1호
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    • pp.24-28
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    • 2009
  • Purpose: This was conducted to assess the need for conventional radiographs of the thoracolumbar spine for routine screening of trauma patients who revealed no spinal trauma on chest and abdominal computed tomography (CT). Methods: We performed a retrospective review of the medical records of trauma patients who underwent conventional radiographs of the thoracolumbar spine following a chest and abdominal CT that revealed no spinal trauma. Results: Two hundred seventy-five trauma patients underwent conventional radiographs of the thoracolumbar spine following a chest and abdominal CT that revealed no spinal trauma. In 274 of the cases, the thoracolumbar spine series was also negative. Conclusion: CT of the chest and abdomen is an adequate evaluation of the thoracolumbar spine in trauma patients who require routine thoracolumbar spine screening, making subsequent conventional radiographs of the thoracolumbar spine unnecessary.

요 세포 검사의 진단적 가치; 액상세포검사와 고식적 방법의 비교 (Diagnostic Value of Urine Cytology in 236 cases; a Comparison of Liquid-Based Preparation and Conventional Cytospin Method)

  • 이선;박정희;도성임;김윤화;이주희;장성구;박용구
    • 대한세포병리학회지
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    • 제18권2호
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    • pp.119-125
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    • 2007
  • Urine cytology is an important screening tool for urinary tract neoplasms. Liquid-based preparation methods, such as $ThinPrep^{(R)}$, have been introduced for non-gynecological samples. We aimed to assess the diagnostic accuracy of liquid-based preparations in urine cytology by comparing the results of the conventional Cytospin preparation method for the same samples. A total of 236 cases subject to urine cytology were enrolled in this study from January 2005 to December 2005. All cases were subjected to cystoscopy and if a malignancy was suspected, a biopsy was performed. Urine cytology slides were made using the $ThinPrep^{(R)}$ preparation method and the conventional Cytospin and/or direct smear method from the individual samples. The results of urine cytology were compared with the final cystoscopic or histological diagnoses. We analyzed the sensitivity, specificity, positive predictive value, negative predictive value and accuracy of both cytology preparation methods. A total of 236 slides made using the liquid based method were satisfactory for slide quality, whereas 5 slides (2.1%) prepared by conventional methods were unsatisfactory because of air-drying, a thick smear, or a bloody or inflammatory background. The $ThinPrep^{(R)}$ method showed 53.1% sensitivity, 92.6% specificity, a 92,6% positive predictive value, a 94.1% negative predictive value and 85,6% accuracy, while the conventional method showed 51% sensitivity, 98.4% specificity, a 92.6% positive predictive value, a 98.4% negative predictive value and 88,6% accuracy. Although the diagnostic values were equivalent between the use of the two methods, the quality of the cytology slides and the time consumed during the microscopic examination for a diagnosis were superior for the $ThinPrep^{(R)}$ method than for the conventional method. In conclusion, our limited studies have shown that the use of the liquid based preparation method is beneficial to improve the quality of slides and reduce the duration for a microscopic examination, but did not show better sensitivity, accuracy and predictive values.

농법에 따른 박쥐의 서식지 이용 특성 및 지표종으로써의 활용 가능성 고찰 (Habitat Use Characteristics and Possibility of Bats as Indicator Species of Farming Methods)

  • 전영신;김성철;임춘우;정철운
    • 한국환경과학회지
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    • 제32권10호
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    • pp.685-692
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    • 2023
  • This study was conducted in organic and conventional farming areas to understand the activity and habitat use patterns of bats according to farming methods. A total of 521 bat sounds were recorded, of which approximately 90% sounds were of Pipistrellus abramus. The activity of bats according to farming methods was significantly higher in organic farming areas. Bat activity was the highest for approximately an hour after sunset, and gradually decreased. Buzz calls in bats were also significantly higher in organic farming areas, and buzz calls were identified only in Pipistrellus abramus. Our results suggest that the activity of bats was significantly higher in organic farming areas, and the differences in activity and habitat use of bats according to farming methods show that bats can be used as an indicator species of farming methods.

Conventional and digital impressions for complete-arch implant-supported fixed prostheses: time, implant quantity effect and patient satisfaction

  • Pereira, Ana Larisse Carneiro;Medeiros, Vitoria Ramos;Campos, Maria de Fatima Trindade Pinto;Medeiros, Annie Karoline Bezerra de;Yilmaz, Burak;Carreiro, Adriana da Fonte Porto
    • The Journal of Advanced Prosthodontics
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    • 제14권4호
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    • pp.212-222
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    • 2022
  • PURPOSE. To evaluate and compare the effect of impression type (conventional vs digital) and the number of implants on the time from the impressions to the generation of working casts of mandibular implant-supported fixed completearch frameworks, as well as on patient satisfaction. MATERIALS AND METHODS. 17 participants, 3 or 4 implants, received 2 types of digital impression methods (DI) and conventional (CI). In DI, two techniques were performed: scanning with the scan bodies (SC) and scanning with a device attached to the scan bodies (SD) (BR 10 2019 026265 6). In CI, the making of a solid index (SI) and open-tray impression (OT) were used. The outcomes were used to evaluate the time and the participant satisfaction with conventional and digital impressions. The time was evaluated through the timing of the time obtained in the workflow in the conventional and digital impression. The effect of the number of implants on time was also assessed. Satisfaction was assessed through a questionnaire based on seven. The Wilcoxon test used to identify the statistical difference between the groups in terms of time. The Mann-Whitney test was used to analyze the relationship between the time and the number of implants. Fisher's test was used to assess the patient satisfaction (P<.05). RESULTS. The time with DI was shorter than with CI (DI, $\tilde{x}=02:58$; CI, $\tilde{x}=31:48$) (P<.0001). The arches rehabilitated with 3 implants required shorter digital impression time (3: $\tilde{x}=05:36$; 4: $\tilde{x}=09:16$) (P<.0001). Regarding satisfaction, the DI was more comfortable and pain-free than the CI (P<.005). CONCLUSION. Digital impressions required shorter chair time and had higher patient acceptance than conventional impressions.

움직임 벡터의 1차원 히스토그램을 이용한 비디오 화질 평가 척도 (Video Quality Metric Using One-Dimensional Histograms of Motion Vectors)

  • 한호성;김동오;박래홍;심동규
    • 대한전자공학회논문지SP
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    • 제45권2호
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    • pp.21-28
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    • 2008
  • 본 논문에서는 비디오 화질 평가를 위해 움직임 벡터의 1차원 히스토그램을 비디오의 특징으로 이용하는 새로운 reduced-reference (RR) 평가 방법을 제안하였다. 제안한 화질 평가 방법은 수신단에서 열화 비디오를 재구성하는 대신 비디오 스트림 (video stream)의 파싱 (parsing) 과정에서 움직임 벡터를 직접 얻을 수 있기 때문에 수행시간 면에서 기존의 방법들에 비해 효율적이다. 또한 송신단에서는 입력 비디오 영상 전체에 대해 누적된 움직임 벡터의 1차원 히스토그램을 보내기 때문에 데이터량 측면에서도 효율적이다. 여기서, 기존의 방법들이 영상 한 장씩에 대해서 평가를 했던 것과 달리 제안한 방법에서는 전체 영상에 대해 누적된 움직임 벡터의 1차원 히스토그램을 사용하였다. 히스토그램의 유사도를 측정하기 위해 히스토그램 인터섹션 (histogram intersection)과 히스토그램 파이 (histogram difference)을 사용하였다. 여러 가지 비트율 (bit rate), 영상크기, 프레임율 (frame rate)로 코딩된 비디오 클립 52개에 대해 제안한 방법과 기존의 방법들을 비교하였고, 제안한 방법의 효율성을 기존 방법들과의 비교 실험을 통해 보였으며, 실험 결과를 통해, 제안한 방법이 기존의 방법들보다 mean opinion score (MOS)와 유사함을 보였다.