• Biotechnology and Bioprocess Engineering:BBE
• /
• v.8 no.2
• /
• pp.117-125
• /
• 2003

Experimental and theoretical works were performed for the separation of large polyelectrolyte, such as DNA, in a column packed with gel particles under the influence of an electric field. Since DNA quickly orient in the field direction through the pores, this paper presents how intraparticle convection affects the residence time distribution of DNAs in the column. The concept is further illustrated with examples from solid -liquid systems, for example, from chromatography Showing how the column efficiency is improved by the use of a n electric field. Dimensionless transient mass balance equations were derived, taking into consideration both diffusion and electrophoretic convection. The separation criteria are theoretically studied using two different Peclet numbers in the fluid and solid phases. These criteria were experimentally verified using two different DNAs via electrophoretic mobility measurements. which showed how the separation position of the DNAs varies in the column in relation to the Peg/Pef values of an individual DNA. The residence time distribution was solved by an operator theory and the characteristic method to yield the column response.

• Journal of Korean Port Research
• /
• v.9 no.1
• /
• pp.45-56
• /
• 1995

Investigation of the physical behavior of dredged material disposal in coastal water includes estimations of water column concentration in the receiving water, exposure time, the initial deposition pattern as well as thickness of material at the dumping fields near the estuary area. Calculation based on vertical setting and horizontal advection of single particles ignore the effects of bulk properties of the disposed material, vertical and horizontal diffusion, and material dilution due to the entrainment of ambient water during descent. This paper focuses on the spatial and temporal changes in the dumping fields for the water column and bottom at a hypothetically confined coastal water, where the ambient time-invariant velocity and density profiles are applied, within the initial time period following the instantaneous release of the dredged material. This model accounts the behavior of material after release divided into three phases: convective descent, dynamic collapse and long-term passive dispersion

• 한국전산유체공학회:학술대회논문집
• /
• 2004.03a
• /
• pp.71-76
• /
• 2004

An extension of our recently developed locally linear reconstruction scheme to 2 dimensional incompressible flow solver is presented. The solver is based on a semi-implicit fractional step method in which the convective term is discretized by Adams-Bashforth method and the diffusion term by Crank-Nicolson method. Several numerical examples are tested to demonstrate the mesh type independent accuracy of the solver, which include decaying vortex flow, square cavity flow, and flow around a circular cylinder. The above examples are solved on quadrilateral or hybrid meshes. For all numerical examples, we obtained reasonable results.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.32 no.8
• /
• pp.624-632
• /
• 2008

In this research, we developed new PDMS-glass based microbiochip consisted of the micromixer and microreactor for DNA ligation. The micromixer was composed of a straight channel integrated with nozzles and pillars, and the microreactor was composed of a serpentine channel. We coated the PDMS chip surface with the 0.25wt.% PVP solution to prevent the bubble generation which was caused by the hydrophobicity of the PDMS. The new micomixer was passive type and the mixing was enhanced by a convective diffusion using the nozzle and pillar. The 10.33mm long micromixer showed the good mixing efficiency of 87.7% at 500 l/min flow rate. We could perform the DNA ligation successfully in the microbiochip, and the ligation time was shortened from 4 hours in conventional laboratory method to 5 min in the microbiochip.

• Journal of Ocean Engineering and Technology
• /
• v.11 no.4
• /
• pp.141-151
• /
• 1997

This paper deals with a numerical study of flow-guider applied to controlling current in a bay. Two dimensional numerical model for tidal currents based on the depth averaged equation is developed and standard k-.epsilon. model is adopted to determine the turbulence diffusion. Equations are described in a generalized coordinate system to be implemented by non-staggered grid system and discretized by using finite volume method. Unsteady flow is simulated by fully implicit scheme. Hybrid scheme and central differencing are used to compute the convective terms and source terms, respectively. The tidal current in a rectangular bay is simulated and it gives satisfactory results. The realistic and distinct models of a large structure placed in bay are also exemplified with or without flow-guiders. The simulation results show that the flow-guider gives the residual tidal current in the bay by the different flux with respect to the direction of tidal current.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.19 no.7
• /
• pp.1720-1730
• /
• 1995

Finite-difference analysis was conducted to study the natural convection of a stably stratified salt-water solution in an annulus due to lateral heating. The main purpose of this study is to examine in detail the multi-layered flow structure. Calculation was thus made for R $a_{\eta}$=2*10$^{5}$ and 6.5*10$^{5}$ . Formation of layered flow structure, merging process of layers, the corresponding temperature and concentration distributions, Nusselt number variations with time are examined. Numerical results show that in each layer, the temperature profile looks 'S`-shaped and the concentration profile is uniform due to the convective mixing. The formation of the roll and the layer is governed by natural convection due to the temperature gradient and the merging process of the layer by diffusion of the concentration.ation.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.20 no.12
• /
• pp.3917-3925
• /
• 1996

A numerical analysis on three dimensional turbulent incompressible flows through linear cascades of turbine rotor blades with high turning angles has been performed by using a generalized k-.epsilon. model which is a high Reynolds number form and derived by RNG(renormalized group) method to account for the variation of the rate of strain. A second order upwind scheme is used to suppress numerical diffusion in approximating the convective terms. Body-fitted coordinates are adopted to represent the complex blade geometry accurately. For the case without tip clearance, velocity vectors and static pressure contours are shown to be in good agreement with previous experimental results. For the case with tip clearance, the effects of the passage vortex and tip clearance flow on the total pressure loss as well as their interactions are discussed.

• Journal of the Korean Society of Combustion
• /
• v.3 no.2
• /
• pp.51-63
• /
• 1998

Air-assisted atomizer flames are investigated numerically to study spray structures in nonburning and burning conditions based on experimental data. A PDA is used to measure droplet size, velocity, and number density for both nonburning and burning spray. Computations utilize time-averaged gas-phase equations and $k-{\varepsilon}$ turbulence model for simplicity. The major features of the liquid-phase model are that a SSF approach is used to represent the effect of gas-phase turbulence on droplet trajectories and vaporization, an infinite-diffusion model is employed to represent the transient liquid-phase process. Computation and experiment results show that the droplet acceleration and evaporation proceed quickly in near the atomizer, characterizing high number densities and a strong convective effect. The primary combustion zone, however, is dorminated by the gas phase reaction and exhibits a sheath combustion.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.5 no.5
• /
• pp.332-339
• /
• 2000

Experimental and theoretical works were performed for the separation of large polyelectrolytes such as DNA in the column packed with gel particles under an electric field. This paper shows how intraparticle convection effects the separation of DNAs in the column because DNAs quickly oriented through the pores in the field direction. Dimensionless transient mass balance equations were derived considering diffusion and electrophoretic convection. The separation criteria is theoretically studied using two different Peclet numbers in the fluid and solid phases and these criteria were verified uing two different DNAs by electrophoretic mobilities measured experimentally, showing how the separation position of DNAs varies in the column according to values of Pe(sub)f/Pe(sub)g of individual DNA. Governing equations are simultaneously solved by operator theoretic and characteristic methods to yield the column response.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.7 no.3
• /
• pp.521-527
• /
• 1995

The average particle deposition velocity toward a vertical wafer surface in a vertical airflow chamber was measured by a wafer surface scanner(PMS Model SAS-3600). Polystyrene latex(PSL) spheres with diameters between 0.3 and $0.8{\mu}m$ were used. To examine the effect of the airflow velocity on the deposition velocity, experiments were conducted for three vertical airflow velocities ; 20, 30, 50cm/s. Experimental data of particle deposition velocity were compared with those given by prediction model suggested by Liu and Ahn(1987).