• Molecular & Cellular Toxicology
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• 제3권4호
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• pp.292-298
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• 2007

Zinc plays indispensable roles in metabolism, including cell growth, apoptosis, proliferation and differentiation. Kidneys are target organs for various regulators of mineral metabolism, and play a key role in zinc balance. To investigate the zinc uptake efficiency, we examined the zinc uptake and accumulation level in vivo and in vitro study. Plasma zinc concentration was peaked out at 1 hr after oral zinc administration. The renal zinc level was peaked out at 12 hr after oral zinc administration, and it was the highest in 40 mg/kg Zn-Asp administrated group in comparison with other groups. In addition, the m-RNA expression level of zinc transporter-1 (ZnT-1), zinc transporter-2 (ZnT-2) and high-affinity L-aspartate transporter (EAAT-3) in Zn-Asp administered group were increased compared with control groups and $ZnSO_4$ group. In order to investigate the intracellular zinc uptake mechanism, we performed the in vitro study by using human embryonic kidney cell line, HEK 293. Intracellular zinc level was peaked out at 3 hr after zinc treatment. In the same way, the mRNA expression level of ZnT-1 and EAAT-3 were increased compared with control group. This study showed that Zn-Asp is effective the zinc uptake into the kidney by increasing the zinc transporter expression.

• 한국게임학회 논문지
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• 제13권5호
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• pp.5-18
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• 2013

온라인 게임에서 게임통화의 순환과 관리는 단지 게임 내의 경제 밸런스 유지뿐만 아니라 상용화 게임으로서의 성공 여부를 판가름 짓는 중요한 요소로 인식되고 있다. 그리고 게임 머니의 발생과 흐름은 게임의 컨텐츠와 재미요소, 기획과 레벨 디자인이라는 요소들과도 밀접한 상관관계를 가지고 있다. 본 연구는 이러한 맥락에서 온라인 게임 경제, 특히 게임 통화관리라는 주제에 대해 이론적이고 실무적인 분석을 통해 다각적인 방향으로 접근을 시도했고 그것이 상용화 정책과 매출에 미치는 연관성에 대해서도 살펴보고 있다. 아울러 본 논문은 향후 '게임통화 관리모델'에 대한 다양한 연구와 접근방법의 유용한 토대로서 기여하고자 하며 게임제작과 관리운영에 실리적으로 활용가능한 연구결과를 이끌어 내고자 했다.

• 융합경영연구
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• 제4권4호
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• pp.29-35
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• 2016

This study has focused on the transfer of infants and Toddlers-protecting responsibilities from families to infants and Toddlers education agencies due to an increase of a double-income family and nuclear family. It is because there is insufficient safe play space from the moment when infants and Toddlers return from infants and Toddlers education agencies to home due to a high crowding of housing and increase of vehicle, and there can be difficulties to take responsibilities of infants and Toddlers safety with a mere recognition and effort of parent. Thus, to suggest a safety education through mutual contact between the communities and agencies supporting parent role in a level of social welfare, its potential has been identified through a frequency analysis on 60 participants of experimental group who have trained a safety education and 60 participants of control group who have not trained a safety education. As a result of frequency analysis, the increase in safety condition in Korea, safety and development of infants and Toddlers, safety of play materials, and safety knowledge on general part of facility safety has detected and the practice of safety and safety consciousness has also identified to be more effective. The results of this study has shown a potential to verify its efficacy if it is verified through an elaborative model of safety education based on parents of young infants and Toddlers family.

• The Korean Journal of Physiology and Pharmacology
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• 제1권1호
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• pp.45-53
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• 1997

In humans and many animal models with chronic progressive renal diseases, angiotensin-converting enzyme (ACE) inhibitor markedly attenuates the progression of nephropathy. Several studies have reported augmented gene expression and redistribution of renal renin in partial nephrectomized rats. Although precise mechanism(s) is not known, the renin-angiotensin system (RAS) may play an important role in the progression of renal diseases. Thus, this study was undertaken to examine the gene expression of renal renin, angiotensinogen, and $AT_1$ subtypes ($AT_{1A}$ and $AT_{1B}$) in rats with diabetic nephropathy, and the influences of lipopolysaccharide (LPS)-induced septicemia on the gene expression. Four weeks after streptozotocin (STZ) treatment (55 mg/kg, i.p.), rats were randomly divided into LPS-treated (1.6 mg/kg, i.p.) and control rats. At 6 hours after LPS treatment, the rats were killed and the kidney was removed from each rat. Northern blot and reverse transcription-polymerase chain reaction (RT-PCR)techniques were used to detect mRNA expression. STZ treatment markedly attenuated body weight gain and significantly increased blood glucose level. Renal renin content (RRC) was significantly decreased in the STZ-treated rats compared to that in control rats. The renal ACE activity between STZ-treated and control rats was not significantly different. Renal renin mRNA level was prominently increased, while angiotensinogen and $AT_{1A}$ mRNA levels were slightly decreased in STZ-treated rats compared to those in controls. $AT_1$B mRNA level did not differ in both groups. Acute LPS treatment did not show any significant changes of mRNA levels of intrarenal RAS components in both groups. These results suggest that intrarenal RAS components were differentially regulated in STZ-treated diabetic rats. Further studies are required to evaluate the relationship between intrarenal RAS and other vasomodulatory systems.

• 로봇학회논문지
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• 제13권3호
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• pp.151-156
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• 2018

Military robots are expected to play an important role in the future battlefield, and will be actively engaged in dangerous, repetitive and difficult tasks. During the robots perform the tasks a human operator controls the robots in a supervisory way. The operator recognizes battlefield situations from remote robots through an interface of the operator control center, and controls them. In the meantime, operator workload, controller interface, robot automation level, and task complexity affect robot operability. In order to assess the robot operability, we have developed ROSim (Robot Operational Simulator) incorporating these operational factors. In this paper, we introduce the results of applying ROSim experimentally to the assessment of reconnaissance robot operability in a battle field. This experimental assessment shows three resulting measurements: operational control workload, operational control capability, mission success rate, and discuss its applicability to the defense robot research and development. It is expected that ROSim can contribute to the design of an operator control center and the design analysis of a human-robot team in the defense robot research and development.

• 가정과삶의질연구
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• 제25권1호
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• pp.29-43
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• 2007

Effectiveness of Long-term Group-counseling in Improving Emotional Intelligence and Prosocial Behavior in Preschooler The purpose of this study was to assess the effectiveness of long-term group-counseling in preventing children's maladjustment at school. for this study, six children were selected for an experimental group and another six for a control group. They were tested using the Emotional Intelligence Rating System and the Prosocial Behavior Skill Situations Measure for Preschool Level. In addition to quantitative analysis, a qualitative analysis was conducted to examine group processes and changes in each child. The experimental group participated insocio-drama, group art therapy, and game play therapy whereas the control group did not receive any treatment. In order to examine the impact of the intervention, pre-program tests and post-program tests were conducted. The results were as follows. First, the children's emotional intelligence was improved with group counseling. There was a significant difference in emotional intelligence between the experimental and the control groups. Second, children's prosocial behavior ability alsoimproved. There was a significant difference in social ability between the experimental and the control groups.

• 운동과학
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• 제26권3호
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• pp.229-237
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• 2017

PURPOSE: This study was to investigate the Glycolysis mediated sarcoplasmic reticulum (SR) $Ca^{2+}$ signal regulates mitochondria $Ca^{2+}$ during skeletal muscle contraction by using glycolysis inhibitor. METHODS: To examine the effect of Glycolysis inhibitor on SR and mitochondria $Ca^{2+}$ content, we used skeletal muscle fiber from gastrocnemius muscle. 2-deoxy glucose and 3-bromo pyruvate used as glycolysis inhibitor, it applied to electrically stimulated muscle contraction experiment. Intracellular $Ca^{2+}$ content, SR, mitochondria $Ca^{2+}$ level and mitochondria membrane potential (MMP) was detected by confocal microscope. Mitochondrial energy metabolism related enzyme, citric acid synthase activity also examined for mitochondrial function during the muscle contraction. RESULTS: Treatment of 2-DG and 3BP decreased the muscle contraction induced SR $Ca^{2+}$ increase however the mitochondria $Ca^{2+}$ level was increased by treatment of inhibitors and showed and overloading as compared with the control group. Glycolysis inhibitor and thapsigargin treatment showed a significant decrease in MPP of skeletal muscle cells compared to the control group. CS activity significantly decreased after pretreatment of glycolysis inhibitor during skeletal muscle contraction. These results suggest that regulation of mitochondrial $Ca^{2+}$ levels by glycolysis is an important factor in mitochondrial energy production during skeletal muscle contraction CONCLUSIONS: These results suggest that mitochondria $Ca^{2+}$ level can be regulated by SR $Ca^{2+}$ level and glycolytic regulation of intraocular $Ca^{2+}$ signal play pivotal role in regulation of mitochondria energy metabolism during the muscle contraction.

• 한국조경학회지
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• 제37권2호
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• pp.47-61
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• 2009

본 연구에서는 어린이놀이시설의 안전관리를 합리적으로 하기 위해 현재 적용되고 있는 어린이놀이시설 안전관리 관련법규를 조사 분석하여 문제점을 밝히고 개선방안을 제안하는데 목적이 있다. 연구를 위해 어린이놀이시설 안전관리의 기본법인 "어린이놀이시설 안전관리법" 및 "품질경영 및 공산품안전관리법"과 "도시공원 및 녹지 등에 관한 법률", "주택법", "아동복지법", "영유아보육법", "건설기술관리법" 등을 분석대상으로 하여 첫째, 어린이놀이시설 안전관리와 관련된 법률의 조항을 도출하여 분석하고, 둘째, 조경산업의 활동영역과 일치하게 어린이놀이시설의 제품개발, 설계, 시공, 유지관리의 전체적인 흐름에 기초하여 단계별로 법규의 적용 특성과 불합리한 문제점을 밝혀냈다. 마지막으로 바람직한 어린이놀이시설 안전관리체계를 운영하기 위한 개선방안을 제시하였으며, 연구의 결과는 다음과 같다. 1. 분석 결과 "어린이놀이시설 안전관리법"을 제정하고 안전검사기준 및 안전인증기준을 준비하면서 전문성이 있는 조경분야의 참여가 부족하였고, 소량 다품종의 특성을 갖는 어린이놀이시설의 특성과 어린이놀이행태에 대한 고려가 미흡하였다. 또한, 안전인증의 정기검사기간이 짧고, 사소한 변화에도 재인증을 받아야 하는 불합리한 점이 나타났으며, 제품검사와 공장심사의 중복과 수출입 놀이시설의 안전인증 중복, 설치검사의 "건설기술관리법"에 의한 품질관리 및 감리규정과 중복, 기 설치된 놀이시설의 설치검사 소급적용, 안전검사기관의 부족이라는 문제점이 도출되었다. 2. 바람직한 어린이놀이시설 안전관리를 위해 "어린이놀이시설 안전관리법"을 유지 발전시키고 관련법규와 상호조정을 통하여 종합적인 어린이놀이시설 안전관리체계의 구성이 필요하다. 또한, 안전인증 검사 기준을 우리나라의 실정에 맞도록 정비하는 동시에 국제적인 수준에 부합되도록 해야 하며, 안전인증 검사의 절차 방법을 합리화하여 안전인증 및 검사절차가 중복되거나 과다한 비용이 소모되지 않도록 하고 안전검사기관의 전문성 향상 및 안전검사기관의 확대가 필요하다. 향후 종합적인 어린이놀이시설 안전관리체계를 발전시키기 위해서는 현장에서 발생하는 문제점을 분석하여 관련법규의 재정비 및 법조항의 개정에 관한 심층적인 연구가 필요하다.

• Archives of Pharmacal Research
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• 제23권6호
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• pp.620-625
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• 2000

The mechanisms of liver injury from cold storage and reperfusion are not completely under-stood. The aim of the present study was to investigate whether the inactivation of Kupffer cells (KCs) by gadolinium chloride ($GdCl_3$) modulates ischemia-reperfusion injury in the rat liver. Hepatic function was assessed using an isolated perfused rat liver model. In livers subjected to cold storage at $4^{\circ}C$ in University of Wisconsin solution for 24 hrs and to 20 min rewarm-ing ischemia, oxygen uptake was markedly decreased, Kupffer cell phagocytosis was stimulated, releases of purine nucleoside phosphorylase and lactate dehydrogenase were increased as compared with control livers. Pretreatment of rats with $GdCl_3$) , a selective KC toxicant, suppressed kupffer cell activity, and reduced the grade of hepatic injury induced by ischemia-reperfusion. While the initial mixed function oxidation of 7-ethoxycoumarin was not different from that found in the control livers, the subsequent conjugation of its meta-bolite to sulfate and glucuronide esters was suppressed by ischemia-reperfusion, CdCl$_3$restored sulfation and glucuronidation capacities to the level of the control liver. Our findings suggest that Kupffer cells could play an important role in cold/warm ischemia-reperfusion hepatic injury.

• The Korean Journal of Physiology
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• 제27권1호
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• pp.37-49
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• 1993

Ventilatory responses to inhaled $CO_2$ were measured during continuous negative pressure breathing (CNPB) in awake dogs. End expiratory lung volume (EELV) decreased linearly with pressure level during CNPB (correlation coefficient= 0.81, p<0.005) during air breathing. When CNPB was applied during 5% $CO_2$ inhalation, the decrease in EELV was not significantly different (p<0.5) from that during air breathing. As a result of a lowered EELV, tidal volume ($V_T$) significantly decreased by 22% and breathing frequency ($f_B$) increased by 68% in the steady state during air breathing (p<0.0001). These responses were similar during 5% $CO_2$ inhalation, thus the $CO_2$ response curve measured during CNPB shifted upward without a change in sensitivity (p>0.05). These results indicate additive effects of CNPB and $CO_2$ inhalation. The degree of hyperventilation during CNPB at eupnea was estimated to be 63% of that during control ventilation and was significantly greater than zero (p<0.0001), which suggests an alveolar hyperventilation due to CNPB. These results suggest that the mechanical alterations associated with n decrease in lung volume could play an important role in ventilatory control independently of chemical regulation of breathing. Thus, exercise hyperpnea, which is associated with a lowered functional residual capacity (FRC), may in part be explained by this mechanical stimulation of breathing.