• Title/Summary/Keyword: Continuous flow system

Search Result 506, Processing Time 0.031 seconds

Continuos-Flow culture of Hepatocytes in Sugar-derivatized poly (lactide-co-glycolide) Scaffolds Prepared by Gas-foaming/salt-leaching Method

  • Yun, Jun-Jin;Park, Tae-Gwan
    • 한국생물공학회:학술대회논문집
    • /
    • 2000.04a
    • /
    • pp.141-144
    • /
    • 2000
  • Highly open porous polymer matrices are required for high density cell seeding, efficient nutrient, and oxygen supply to the cells cultured in the three dimensional matrices. However, there are severe problems of mass transfer limitations within the cell/scaffolds culture system. Thus we hypothesize that continuos-flow culture conditioning of cells with the scaffolds may improve the cell viability and the differentiated function. In this study, we fabricated porous PLGA scaffolds by using gas-foaming/salt-leaching method as previous described. Viscous PLGA gel paste contains ammonium bicarbonate particulates, acting as a gas-foaming agent as well as a salt-leaching porogen, were cast into Teflon mold and dried. Ammonium bicarbonate salt upon contact to an acidic aqueous solution evloves gaseous ammonia and carbon dioxide by itself. And we conjugated galactose moiety [AGA; $N-(aminobuty1)-O-{\beta}-D-galactopyranosyl-(1{\rightarrow}4)-D-glucoamide]$ to the terminal end group of a PLGA to increase the cell adhesion and matain the differentiated function of hepatocytes. Cell-seeded scaffolds were secured in a flow bioreactor chamber and exposed to continuous flow at 5 ml/min. As a result of our study, the high yield of hepatocytes attachment was accomplished by increasing the concentration of PLGA-AGA conjugate in polymer scaffolds and cells in the scaffolds under continuos flow condition maintained a high level of viability and albumin secretion rate of cultured hepatocytes showed a higher level that of control groups.

  • PDF

Phenomenological Damping Flow Modeling and Performance Evaluation for a Continuous Damping Control Damper Using MR Fluid (MR 유체를 이용한 연속 감쇠력 가변형 댐퍼를 위한 감쇠유동의 현상학적 모델링과 성능평가)

  • Park, Jae-Woo;Jung, Young-Dae
    • Journal of the Korean Society for Precision Engineering
    • /
    • v.25 no.3
    • /
    • pp.73-82
    • /
    • 2008
  • Recently MR CDC damper has been applied to semi-active suspension control system gradually. Compared to former hydraulic CDC damper, it has rapid time response performance as well as simple internal structure and wide range of damping force. In order to develop control logic algorithm which enables to take maximum advantage of unique characteristics of MR CDC damper, it is inevitable to perform a thorough investigation into its nonlinear performance. In many previous researches, MR fluid model was either simply assumed as Bingham Plastic, or a phenomenological model based on experiment was established instead to predict damping performance of MR CDC damper. These experimental flow model which is not based on flow analysis but intentionally built to fit damping characteristics, may lead to totally different results in case of different configuration or structure of MR CDC damper. In this study, a generalized flow formula from mathematical flow model of MR fluid for annular orifice is derived to analyze and predict damping characteristics when current is excited at piston valve.

An Analysis of the Relationship between Rainfall and Recession Hydrograph for Base Flow Separation (기저유출 분리를 위한 강우와 감수곡선간의 상관해석)

  • 이원환;김재한
    • Water for future
    • /
    • v.18 no.1
    • /
    • pp.85-94
    • /
    • 1985
  • A method is developed for the separation of the major base flow in a river hydrograph combining the numerical techniques and the empirical methods. The linearized Boussinesq equation and the storage function are used to obtain the base flow recession. The shape of base flow curve made by the recharge of the groundwater table aquifer resulting from rainfall in determined by the Singh and Stall's graphical method, and the continuous from for the curve is approximated by the multiple and polynomial regression. this procedure was successfully tested for the separation of base flow and the establishment of hydrograph in a natural watershed. It was found that the direct numerical method applied to the homogeneous linear second order ordinary differential equation system is not suited to obtain the recession curve, and the case that the loss is generated in the partially penetrating stream can not be solved by the method of this study.

  • PDF

Reduced Switch Count Topology of Current Flow Control Apparatus for MTDC Grids

  • Diab, Hatem Yassin;Marei, Mostafa Ibrahim;Tennakoon, Sarath B.
    • Journal of Power Electronics
    • /
    • v.16 no.5
    • /
    • pp.1743-1751
    • /
    • 2016
  • The increasing demand for high voltage DC grids resulting from the continuous installation of offshore wind farms in the North Sea has led to the concept of multi-terminal direct current (MTDC) grids, which face some challenges. Power (current) flow control is a challenge that must be addressed to realize a reliable operation of MTDC grids. This paper presents a reduced switch count topology of a current flow controller (CFC) for power flow and current limiting applications in MTDC grids. A simple control system based on hysteresis band current control is proposed for the CFC. The theory of operation and control of the CFC are demonstrated. The key features of the proposed controller, including cable current balancing, cable current limiting, and current nulling, are illustrated. An MTDC grid is simulated using MATLAB/SIMULINK software to evaluate the steady state and dynamic performance of the proposed CFC topology. Furthermore, a low power prototype is built for a CFC to experimentally validate its performance using rapid control prototyping. Simulation and experimental studies indicate the fast dynamic response and precise results of the proposed topology. Furthermore, the proposed controller offers a real solution for power flow challenges in MTDC grids.

Evaluation of 0ff-gas Characteristics in Vitrification Process of ion-Exchange Resin

  • Park, S. C.;Kim, H. S.;K. H. Yang;C. H. Yun;T. W. Hwang;S. W. Shin
    • Nuclear Engineering and Technology
    • /
    • v.33 no.1
    • /
    • pp.83-92
    • /
    • 2001
  • The properties of off-gas generated from vitrification process of ion-exchange resin were characterized. Theoretical composition and flow rate of the off-gas were calculated based on chemical composition of resin and it's burning condition inside CCM. The calculated off-gas flow rate was 67.9Nm$^3$/h at the burning rate of 40kg/h. And the composition of off-gas was avaluated as $CO_2$(41.4%), steam(40.0%), $O_2$(13.3%), NO(3.6%), and SO$_2$(1.6%) in order. Then, actual flow rate and composition of off-gas were measured during pilot-scale demonstration tests and the results were compared with theoretical values. The actual flow rate of off-gas was about 1.6 times higher than theoretical one. The difference between theoretical and actual flow rates was caused by the in-leakage of air to the system, and the in-leakage rate was evaluated as 36.3Nm$^3$/h. Because of continuous change in the combustion parameters inside CCM, during demonstration tests, the concentration of toxic gases showed wide fluctuation. However, the concentration of CO, a barometer of incompleteness of combustion inside CCM, was stabilized soon. The result showed quasi-equilibrium state was achieved two hours after feeding of resin.

  • PDF

Development of Long Term Flow Duration Curves in 4 River Basins for the Management of Total Maximum Daily Loads (수질오염총량관리를 위한 4대강수계 장기유황곡선 작성방안)

  • Park, Jun Dae;Oh, Seung Young
    • Journal of Korean Society on Water Environment
    • /
    • v.29 no.3
    • /
    • pp.343-353
    • /
    • 2013
  • Flow duration curve (FDC) can be developed by linking the daily flow data of stream flow monitoring network to 8-day interval flow data of the unit watersheds for the management of Total Maximum Daily Loads. This study investigated the applicable method for the development of long term FDC with the selection of the stream flow reference sites, and suggested the development of the FDC in 4 river basins. Out of 142 unit watersheds in 4 river basins, 107 unit watersheds were shown to estimate daily flow data for the unit watersheds from 2006 to 2010. Short term FDC could be developed in 64 unit watersheds (45%) and long term FDC in 43 unit watersheds (30%), while other 35 unit watersheds (25%) were revealed to have difficulties in the development of FDC itself. Limits in the development of the long term FDC includes no stream monitoring sites in certain unit watersheds, short duration of stream flow data set and missing data by abnormal water level measurements on the stream flow monitoring sites. To improve these limits, it is necessary to install new monitoring sites in the required areas, to keep up continuous monitoring and make normal water level observations on the stream flow monitoring sites, and to build up a special management system to enhance data reliability. The development of long term FDC for the unit watersheds can be established appropriately with the normal and durable measurement on the selected reference sites in the stream flow monitoring network.

Experimental Thermal Analysis of Hydraulic System in a Special Vehicle (특장차량 유압시스템 내 열적 특성 분석)

  • Choi, Yu Hyun;Lee, Sang Ho
    • Journal of the Korean Society of Mechanical Technology
    • /
    • v.13 no.4
    • /
    • pp.85-91
    • /
    • 2011
  • Experimental analysis has been carried out to investigate thermal characteristics of hydraulic system in special vehicles. Hydraulic system performance is largely influenced by oil temperature, and there are considerable performance decline and malfunctions in the system for high temperature conditions caused by heavy load and continuous operation. Transient oil temperature and pressure variation are analyzed and heat generation rates in the several main system parts are compared for various flow rates. With the start of system operation oil temperature gradually increases, and viscosity deceases by about 70% as temperature increases from $20^{\circ}C$ to $80^{\circ}C$. Operation pressure in the hydraulic system decreases with oil temperature, and its variation rate becomes less steep as oil temperature increases. Heat generation rate in hydraulic pump also depends on the oil temperature, and it reaches maximum near $50^{\circ}C$. These results in this study can be applied to optimal design of efficient hydraulic system in special vehicles.

On The Security of RFID-based Monitoring Mechanism for Retail Inventory Management

  • Chen, Yu Yi;Jan, Jinn Ke;Tsai, Meng Lin;Ku, Chun Ching;Huang, Der Chen
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • v.6 no.2
    • /
    • pp.515-528
    • /
    • 2012
  • The aim of this article is to provide a study on the issue of inventory inaccuracy and to show the manner in which RFID technology can improve the inventory management performance. The objective of inventory control is to monitor the stock flow of merchandises in order to understand the operating profit and loss. A proper mechanism of inventory control could be made to help the profitability. As RFID is applied to inventory control, it can improve efficiency, enhance accuracy and achieve security. In this paper, we introduce the evolution of different mechanisms of inventory control with RFID system-counting method, collect-all method, and continuous monitoring method. As for improving the accuracy of inventory check during business hours, continuous monitoring is the solution. We introduce the infrastructure of the RFID inventory management system based on M2M architecture can make the inventory be efficiently monitored with instant warnings.

Numerical Study of Effect of counter-pulsation on Hemodynamic Response in the ECLS (체외생명구조장치에서 역박동 방법이 혈류역학 응답에 미치는 영향에 대한 수치적 연구)

  • Kim, In-Su;Lim, Ki-Moo;Choi, Seoung-Wook;Jun, Hyung-Min;Shim, Eun-Bo
    • Proceedings of the KSME Conference
    • /
    • 2008.11a
    • /
    • pp.1660-1664
    • /
    • 2008
  • Extra-corporeal Life Support System (ECLS) is the device used in emergency cases to substitute a extracorporeal circulation in open heart surgery, cardiac arrest or in acute cardiopulmonary failure. To obtain the effect of counter-pulsation on hemodynamic response in the ECLS quantitatively, we developed cardiovascular model which consists of 12 compartment model of heldt et al. and 3 compartment model of Schreiner et al. based on windkessel approximation. We compared coronary perfusion, arterial pulse pressure, cardiac output, and left ventricular pressure-volume diagram according to flow configuration such as counter-pulsation, copulsation, and continous flow. When counter-pulsation was applied, 5% higher coronary perfusion, 26% lower pulse pressure, and 2% higher cardiac output than copulsation condition were calculated. We conclude that counter-pulsation configuration in the ECLS is hemodynamically more stable than copulsation and influences the positive effect to recover ventricles.

  • PDF

A Lip-reading Algorithm Using Optical Flow and Properties of Articulatory Phonation (광류와 조음 발성 특성을 이용한 립리딩 알고리즘)

  • Lee, Mi Ae
    • Journal of Korea Multimedia Society
    • /
    • v.21 no.7
    • /
    • pp.745-754
    • /
    • 2018
  • Language is an essential tool for verbal and emotional communication among human beings, enabling them to engage in social interactions. Although a majority of hearing-impaired people can speak; however, they are unable to receive feedback on their pronunciation most of them can speak. However, they do not receive feedback on their pronunciation. This results in impaired communication owing to incorrect pronunciation, which causes difficulties in their social interactions. If hearing-impaired people could receive continuous feedback on their pronunciation and phonation through lip-reading training, they could communicate more effectively with people without hearing disabilities, anytime and anywhere, without the use of sign language. In this study, the mouth area is detected from videos of learners speaking monosyllabic words. The grayscale information of the detected mouth area is used to estimate a velocity vector using Optical Flow. This information is then quantified as feature values to classify vowels. Subsequently, a system is proposed that classifies monosyllables by algebraic computation of geometric feature values of lips using the characteristics of articulatory phonation. Additionally, the system provides feedback by evaluating the comparison between the information which is obtained from the sample categories and experimental results.