• Title/Summary/Keyword: Continuous flow

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In vitro Glutathione Production using Mixed Cells in an Aerated Slurry Bioreactor (혼합세포를 이용한 Aerated Slurry Bioreactor에서의 in vitro Glutathione 생산)

  • Go, Seong-Yeong;Gu, Yun-Mo
    • KSBB Journal
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    • v.14 no.4
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    • pp.445-451
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    • 1999
  • Glutathione production was carried out using mixed cells of E. coli TG1/pDG7 $\alpha$ and bakers yeast in an Aerated Slurry Bioreactor. Glutathione-producing enzymes were stable for 34 hours, yielding 4.6 mM glutathione in suspension reaction. Glutahione production with high density mixed cells was studied as a function of flow rate in an Aereated Slurry Bioreactor. Glutathione concentration was higher than that in suspension reaction for 32 hours at the substrate feeding rate of 5.2 mL/hr with cell recycle in continuous Aerated Slurry Bioreactor. It was for 42 hours at 2.6 mL/hr and 22 hours at 5.2 mL/hr without cell recycle. Glutahione productivity was 25.7 mg/g wet $cell{\cdot}hr$ at the substrate feeding rate of 10.4 mL/hr with cell recycle, but 5.28 mg/g wet $cell{\cdot}hr$ at 5.2 mL/hr and 1.65 mg/g wet $cell{\cdot}hr$ at 2.6 mL/hr without cell recycle. Effective production time increased from 25 to 45 hours, by using a surfactant, tween 80. As a purfing gas, nitrogen was tested instead of air to avoid a possible oxidizing effect on glutathione-producing enzymes, resulting in the increase of effective production time to 40 hours.

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Biofilter Model for Robust Biofilter Design: 1. Adsorption Behavior of the Media of Biofilter (강인한 바이오필터설계를 위한 바이오필터모델: 1. 바이오필터 담체의 흡착거동)

  • Lee, Eun Ju;Seo, Kyo Seong;Jeon, Wui-Sook;Lim, Kwang-Hee
    • Korean Chemical Engineering Research
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    • v.50 no.1
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    • pp.149-154
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    • 2012
  • The adsorption and desorption behavior of biofilter-medium was investigated on the performance of an adsorption column. Continuous flow-isothermal adsorption experiments were performed to treat waste air containing such a VOC as ethanol under the same condition of > 90% relative humidity as the condition of the feed to a biofilter process. In case of feeding waste air containing ethanol of 1,000 ppmv (or 2,050 mg ethanol/$m^3$) to the adsorption system at the rate of 2 L/min, the onsets of its breakthrough and reaching the state of dynamic equilibrium at the exit had been delayed 10 and 3 times, respectively, later than those at the 1st stage sampling port. Moreover, in case of 2,000 ppmv (or 4,100 mg ethanol/$m^3$), they had been delayed 9 and 3 times, respectively. Thus, regardless of feeding concentration, the ratios of delaying period were observed to be quite consistent each other at the exit of the adsorption column. With regard to the period of desorption, the ratios of delaying period were consistent each other to be 1.5 for both cases. In addition, the effect of microbial activity and sterilization-process was studied on adsorption equilibrium. The ethanol concentration in the vapor phase of vials packed with sterilized granular activated carbon (GAC) was quite consistent to that with unsterilized GAC. However, the ethanol concentrations in the vapor phase of vials packed with unsterilized compost and the unsterilized mixture of GAC and compost were higher than those with sterilized compost and the sterilized mixture of GAC and compost, respectively.

A Study on the Expansion of the Function of the Archives to the Agency Archives (기관 아카이브로의 기록관 기능 확대 방안 연구)

  • Ju, Hyun-Mi;Kim, Ik-Han
    • Journal of Korean Society of Archives and Records Management
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    • v.18 no.1
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    • pp.129-154
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    • 2018
  • It is time to prepare for the imminent development from the field of records management through decentralization to the records management of the new age in accordance with the flow of decentralization. To overcome a centralized record management system, more archives should be established to realize autonomous and decentralized records management. In accordance with the shift to a full-scale electronic record management environment, the appropriateness and effectiveness of the three-phase system of processing-archival-permanent record management based on physical transfer should be reviewed in terms of transfer cost and work efficiency. The archives should function as institutional archives to carry out the continuous volume record management and the autonomous record management at the institution level. This study examined the possibilities and implications of the archives to expand their functions as archives of institutions for the decentralization of record management and information governance orientation. In addition, the study diversified the types of records management institutions as a way to accomplish this and determined a way to design the functions of archives that integrate the current-end-end-end records management. At each level, institutions should set up archives based on their circumstances and aim at information governance at the level of each archive. Moreover, each archive level should establish a horizontal network to govern record information management.

Opto-Electrochemical Sensing Device Based on Long-Period Grating Coated with Boron-Doped Diamond Thin Film

  • Bogdanowicz, Robert;Sobaszek, Michał;Ficek, Mateusz;Gnyba, Marcin;Ryl, Jacek;Siuzdak, Katarzyna;Bock, Wojtek J.;Smietana, Mateusz
    • Journal of the Optical Society of Korea
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    • v.19 no.6
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    • pp.705-710
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    • 2015
  • The fabrication process of thin boron-doped nanocrystalline diamond (B-NCD) microelectrodes on fused silica single mode optical fiber cladding has been investigated. The B-NCD films were deposited on the fibers using Microwave Plasma Assisted Chemical Vapor Deposition (MW PA CVD) at glass substrate temperature of 475 ℃. We have obtained homogenous, continuous and polycrystalline surface morphology with high sp3 content in B-NCD films and mean grain size in the range of 100-250 nm. The films deposited on the glass reference samples exhibit high refractive index (n=2.05 at λ=550 nm) and low extinction coefficient. Furthermore, cyclic voltammograms (CV) were recorded to determine the electrochemical window and reaction reversibility at the B-NCD fiber-based electrode. CV measurements in aqueous media consisting of 5 mM K3[Fe(CN)6] in 0.5 M Na2SO4 demonstrated a width of the electrochemical window up to 1.03 V and relatively fast kinetics expressed by a redox peak splitting below 500 mV. Moreover, thanks to high-n B-NCD overlay, the coated fibers can be also used for enhancing the sensitivity of long-period gratings (LPGs) induced in the fiber. The LPG is capable of measuring variations in refractive index of the surrounding liquid by tracing the shift in resonance appearing in the transmitted spectrum. Possible combined CV and LPG-based measurements are discussed in this work.

A Study on the Sequence Analysis Technique of Urban Landscape Color and Urban Color Characteristics in accordance with Spatial Openness - Focusing on the View of the Daegu Monorail - (도시 경관색채의 시퀀스 분석기법과 공간 개방도에 따른 도시색채 특성연구 - 대구광역시 지상철 조망을 중심으로 -)

  • Koo, Min-Ah
    • Journal of the Korean Institute of Landscape Architecture
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    • v.44 no.6
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    • pp.120-136
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    • 2016
  • This study, views the color of scenery not as a static state, but rather as a continuous sequence of perceptions that incorporates the concept of time. This study derived techniques to quantitatively analyze the flow and data from this sequence. By utilizing this, urban color trends can be based on openness. This is very close to what would be experienced by an actual viewer: it extracted color data and visual amount from frames at 2-second intervals by shooting a video of the color sequence of the city as seen from both the left and right sides from the inside of the monorail (line 3 of the Daegu urban railway). These images were classified by color group, brightness, chroma, high chroma distribution derived techniques such as openness of space, brightness level, clarity level, high-chroma distribution and code, advantage of visual amount, dominant factor exposure, hot and cold color image and dynamic of sequence rhythm. During the derived sequence, the data determines the openness in the visual amount of sky and it was found that the tendency of the colors of the city was opening regression analysis. The more colorful the city is opening the brightness is lowered, the chroma increased slightly, cold colors significantly increased, which also had a very deep relationship with Lynch enclosed proportion, color change of the city trends through the actual scenery could grasp in more detail.

Removal of Off-flavor from Laminaria Japonica by Treatment Process of Supercritical Carbon Dioxide (초임계 이산화탄소 처리 공정에 의한 다시마 유래 이취성분 제거)

  • Park, Jung-Nam;Kim, Ryoung-Hee;Woo, Hee-Chul;Chun, Byung-Soo
    • Clean Technology
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    • v.18 no.2
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    • pp.191-199
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    • 2012
  • In order to reduce or remove off-flavor and volatile organic compounds (VOCs) from Laminaria japonica effectively, continuous treatment process by supercritical carbon dioxide (SC-$CO_2$) was applied. After freeze-drying, Laminaria japonica powdered with $710{\mu}m$ was used. Experiments were carried out at temperature range from 35 to $55^{\circ}C$, and pressure range from 10 to 25 MPa for evaluation of SC-$CO_2$ treatment effect. Flow rate of carbon dioxide used in this reseach was constantly fixed at 26.81 g/min. Before and after treatment of SC-$CO_2$, off-flavor and VOCs from Laminaria japonica were analyzed by gas chromatography-mass spectrometry detector (GC-MSD). Total 47 VOCs emitted from Laminaria japonica were identified before treatment of SC-$CO_2$, major components of seaweed smell (ordor) in Laminaria japonica were identified as alcohols, aldehydes, ester and acids, ketone, halogenated compounds and hydrocarbon. Off-flavor and VOCs in all experimental conditions was reduced or removed after SC-$CO_2$ treatment. Among the experimental conditions, the highest removal yield was at 25 MPa and $55^{\circ}C$.

Application of Very Short-Term Rainfall Forecasting to Urban Water Simulation using TREC Method (TREC기법을 이용한 초단기 레이더 강우예측의 도시유출 모의 적용)

  • Kim, Jong Pil;Yoon, Sun Kwon;Kim, Gwangseob;Moon, Young Il
    • Journal of Korea Water Resources Association
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    • v.48 no.5
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    • pp.409-423
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    • 2015
  • In this study the very short-term rainfall forecasting and storm water forecasting using the weather radar data were implemented in an urban stream basin. As forecasting time increasing, the very short-term rainfall forecasting results show that the correlation coefficient was decreased and the root mean square error was increased and then the forecasting model accuracy was decreased. However, as a result of the correlation coefficient up to 60-minute forecasting time is maintained 0.5 or higher was obtained. As a result of storm water forecasting in an urban area, the reduction in peak flow and outflow volume with increasing forecasting time occurs, the peak time was analyzed that relatively matched. In the application of storm water forecasting by radar rainfall forecast, the errors has occurred that we determined some of the external factors. In the future, we believed to be necessary to perform that the continuous algorithm improvement such as simulation of rapid generation and disappearance phenomenon by precipitation echo, the improvement of extreme rainfall forecasting in urban areas, and the rainfall-runoff model parameter optimizations. The results of this study, not only urban stream basin, but also we obtained the observed data, and expand the real-time flood alarm system over the ungaged basins. In addition, it is possible to take advantage of development of as multi-sensor based very short-term rainfall forecasting technology.

Permeation Properties of Single Gases ($N_2$, $O_2$, $SF_6$, $CF_4$) through PDMS and PEBAX Membranes (PDMS와 PEBAX 분리막을 통한 단일기체($N_2$, $O_2$, $SF_6$, $CF_4$) 투과 특성)

  • Kim, Hanbyul;Lee, Minwoo;Park, Wankeun;Lee, Soonjae;Lee, Hyunkyung;Lee, Sanghyup
    • Membrane Journal
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    • v.22 no.3
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    • pp.201-207
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    • 2012
  • In this study, we investigated permeation of single gas ($N_2$, $O_2$, $CF_4$, and $SF_6$) through flat sheet membrane composed of PDMS (poly-dimethylsiloxane) and PEBAX (polyether block amides). Gas permeation experiment was performed with various feed pressure. Permeability was estimated using permeation flux measured by continuous-flow technique. The permeability of gases except $SF_6$ in PDMS were decreased with the upstream pressure increased. $SF_6$ is much more permeable than $CF_4$, which is due to higher critical temperature of $SF_6$. The permeability decreased in the following order: $O_2$ > $N_2$ > $SF_6$ > $CF_4$. On the other hand, the permeability of gases in PEBAX followed the order: $O_2$ > $N_2$ > $CF_4$ > $SF_6$ which are opposite of the order of kinematic diameter (${\AA}$)($SF_6$ > $CF_4$ > $N_2$ > $O_2$). The $SF_6/CF_4$ pure gas selectivity in PDMS was 2.1 at 0.7 MPa.

A Study of Expressway Tollbooth Metering Effect (고속도로 영업소 미터링 효과에 관한 연구)

  • Im, Jin-Won;Yoon, Jae-Yong;Lee, Eui-Eun;Kim, Kwan-Min
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.10 no.4
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    • pp.1-10
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    • 2011
  • According to the worldwide efforts to reduce greenhouse gases consequent upon climatic change, the field of road traffic is also making diverse efforts to reduce the emissions of greenhouse gases. Among these, the exhaust gases from vehicles, the so-called main culprit of the greenhouse gases will take place the more as delay and tie-up of vehicles ever take place. Accordingly, as a scheme for reducing the delay & tie-up of vehicles, it's possible to bring up the idea of supply of new facilities and management of the existing facilities; recently, a lot more focus is being put on the management of the existing facilities due to enormous amounts of construction cost. In the midst of growing concern for traffic demand management policy, it's about the time we should do research on the tollbooth metering on the expressway whose research is almost non-existent home and abroad. As a traffic demand management policy coming to happen in case of the management of pay expressway like Japan and Korea, this research analyzed the contents of tollbooth metering, its effect and its subsequent convenience. Especially as a tool for effect analysis, this research made an analysis using VISSIM-a micro-simulation tool. As the tollbooth metering promoted, as a part of green traffic promotion strategy, is expected to contribute to improvement in traffic flow and reduction in carbon emissions, etc. It seems that there needs to be continuous research work on the management plan & revitalization plan for maximization of its effect later as well.

Development of a 2 Dimensional Numerical Landscape Evolution Model on a Geological Time Scale (2차원 지질시간 규모 수치지형발달모형의 개발)

  • Byun, Jong-Min;Kim, Jong-Wook
    • Journal of the Korean Geographical Society
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    • v.46 no.6
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    • pp.673-692
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    • 2011
  • Advances in computer technology have enabled us to develop and use numerical landscape evolution models (NLEMs) for exploring the dynamics of geomorphic system from a variety of viewpoints which previously could have not been taken. However, as of yet there have been no trials using or developing NLEMs in Korea. The purpose of this research is to develop a 2 dimensional NLEM on a geological time scale and evaluate its usefulness. The newly developed NLEM (ND-NLEM) treats bedrock weathering as one of the major geomorphic processes and attempts to simulate the thickness of soil. As such it is possible to model the weathering-limited as well as the transport-limited environment on hillslopes. Moreover the ND-NLEM includes not only slow and continuous mass transport like soil creep, but also rapid and discrete mass transport like landslides. Bedrock incision is simulated in the ND-NLEM where fluvial transport capacity is large enough to move all channel bed loads, such that ND-NLEM can model the detachment-limited environment. Furthermore the ND-NLEM adopts the D-infinity algorithm when routing flows in the model domain, so it reduces distortion due to the use of the steepest descent slope flow direction algorithm. In the experiments to evaluate the usefulness of the ND-NLEM, characteristics of the channel network observed from the model results were similar to those of the case study area for comparison, and the hypsometry curve log during the experiment showed rational evidence of landscape evolution. Therefore, the ND-NLEM is shown to be useful for simulating landscape evolution on a geological time scale.