• Title/Summary/Keyword: Continuous Culture

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Studies on the Biological Treatment of Waste Water from Acetaldehyde Plant (아세트 알데히드(특수산업) 폐수의 생물학적 처리)

  • 정기택;서승교;송형익;박임동;방광웅
    • Korean Journal of Microbiology
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    • v.25 no.4
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    • pp.333-338
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    • 1987
  • In order to establish the biological treatment system which can be used for treatment of waste aster from acetaldehyde plant, it was investigated optimum nutrient requirements and growth conditions by mixed culture of Micrococcus roseus AW-6, Micrococcus luteus AW-22, Microbacterium lacticum AW-38 and Microbacterium laevaniformans AW-41 as well as the effect of coagulants and neutralization reagents. Also, it was carried out the continuous culture as well as batch culture to treat the waste water by mixed culture of these strains. The COD removal rate was reached to maximum state for 96hrs culture at pH7.0 and $30^{\circ}C$ NaOH as the neutralization reagents was the most effective, but the coagulants had no effect on the COD remonal rate and the optimum dilution times for treatment were 10 fold. The COD removal rate was also increased by supplimenting 200 ppm $NH_{2}NO_{3}$, 50 ppm $KH_{2}PO_{4}$, 15 ppm $CaCl_{2}$ and 1 ppm $MgSO_{4} \cdot 7H_{2}O $ as additional nutrients. The removal rate coefficient $K_{1}$ on the batch culture was $4.5\times 10^{-6}$, and the detention time for BOD removal rate of 85% was approximately 45hrs. The COD of waste water was reduced to 15% of its initial value by the continuous culture. The COD and BOD of the effluents were to be about 60 ppm and 40 ppm, respectively, and final pH was 7.0.

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A Study on the Development of the Safety Culture Inspection Standards and Process in Korea Railway (우리나라 철도안전문화 측정기준 및 프로세스 개발 연구)

  • Lee, Jong-Seock;Han, Kee-Youl;Heo, Nam-Gyu;Kim, Chan-Soo
    • Proceedings of the KSR Conference
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    • 2011.10a
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    • pp.2548-2556
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    • 2011
  • The safety culture of an organization is the product of individual and group values, attitudes, perceptions, competencies, and patterns of behaviour that determine the commitment to, and the style and proficiency of, an organization's health and safety management. A good safety culture includes effective, appropriate safety management systems; strong safety leadership & commitment from management; participation and involvement of the workforce; and organizational learning and continuous improvement. This paper will introduce the safety culture inspection standards and process in Korea Railway. The main purpose is to get a better understanding of safety culture and to develop measuring tool. First of all we developed the composition factor of safety culture and the question set. And we prepared the base of computerization of safety culture measurement by developing of evaluation standards and weighted value.

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The effect of Organizational Culture on Organizational Justice and Job Performance

  • Moon Jun Kim;Lee Soowook
    • International journal of advanced smart convergence
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    • v.13 no.2
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    • pp.154-165
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    • 2024
  • The purpose of this study is to investigate the relationship between organizational culture (innovation culture, relationship culture, hierarchy culture, task culture) as perceived by organizational members and its impact on organizational justice and job performance. This contributes to providing additional data for the revitalization and development of the organizational system for efficient management and operation measures included in the organization's sustainable management. To this end, the hypothesis established through the traditional process of quantitative research was tested as follows. First, organizational culture showed a positive effect on organizational justice. Second, organizational culture had a positive (+) effect on job performance. Third, organizational justice was significantly analyzed in terms of job performance. In other words, the importance of systematic re-establishment and continuous implementation of organizational culture (innovation culture, relationship culture, hierarchy culture, task culture) and organizational justice consistent with organizational characteristics was emphasized in order to improve job performance, which is the result of organizational competitiveness. In addition, it is the aspect of drawing practical implications for strategic human resource management and human resource development to systematically improve it.

A Study on the Intention of Continuous use of MOOC Applying Self-Determination Theory and Learning Flow Theory : Focused on Differences between Korean and Chinese Culture (자기결정성이론과 학습몰입이론을 적용한 MOOC 지속사용의도에 관한 연구 : 한·중 문화차이 분석)

  • Jin, Qiuxiang;Chi, Yong Duk;Gim, Gwangyong
    • Journal of Information Technology Services
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    • v.17 no.1
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    • pp.121-134
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    • 2018
  • Massive Open Online Course (MOOC) is online education that anyone can register for free and has internet access. MOOC is also called Education Revolution and is spreading rapidly all over the world. Although recent MOOC high-quality classes may enhance the value of MOOC, MOOC learning still needs much research. Since MOOC has low learning completion rate and continuous use rate, various studies on the reasons that learners give up at the beginning of learning have not been tried yet This research is studied for the continuous intention of use of MOOC applying self-determination theory and learning flow theory based on technology acceptance model. In particular, the research are conducted for cultural difference in continuous usage of MOOC between Korean and Chinese. The research results show that self-determination theory applying perceived autonomy, perceived competence, and perceived relatedness and learning flow is useful to explain continuous use of MOOC. The research also shows that Hofstede theory works well in explaining the cultural difference between Korea and China in continuous usage of MOOC. The result shows that korean is more influenced by perceived external motivation like perceived usefulness and chinese is more influenced by internal motivation like learning flow in continuous use of MOOC.

Improved Production of Live Cells of Lactobacillus rhamnosus by Continuous Cultivation using Glucose-yeast Extract Medium

  • Ling Liew Siew;Mohamad Rosfarizan;Rahim Raha Abdul;Wan Ho Yin;Ariff Arbakariya Bin
    • Journal of Microbiology
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    • v.44 no.4
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    • pp.439-446
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    • 2006
  • In this study, the growth kinetics of Lactobacillus rhamnosus and lactic acid production in continuous culture were assessed at a range of dilution rates $(0.05 h^{-1}\;to\;0.40h^{-1})$ using a 2L stirred tank fermenter with a working volume of 600ml. Unstructured models, predicated on the Monod and Luedeking-Piret equations, were employed to simulate the growth of the bacterium, glucose consumption, and lactic acid production at different dilution rates in continuous cultures. The maximum specific growth rate of L. rhamnosus, ${\mu}_{max}$, was estimated at $0.40h^{-1}$I, and the Monod cell growth saturation constant, Ks, at approximately 0.25g/L. Maximum cell viability $(1.3{\times}10^{10}CFU/ml)$ was achieved in the dilution rate range of $D=0.28h^{-1}\;to\;0.35h^{-1}$. Both maximum viable cell yield and productivity were achieved at $D=0.35h^{-1}$. The continuous cultivation of L. rhamnosus at $D=0.35h^{-1}$ resulted in substantial improvements in cell productivity, of 267% (viable cell count) that achieved via batch cultivation.

Application of Information Technologies for Lifelong Learning

  • Poplavskyi, Mykhailo;Bondar, Ihor
    • International Journal of Computer Science & Network Security
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    • v.21 no.6
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    • pp.304-311
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    • 2021
  • The relevance of the research involves outlining the need for modern professionals to acquire new competencies. In the conditions of rapid civilizational progress, in order to meet the requirements of the labor market in the knowledge society, there is a readiness for continuous training as an indicator of professional success. The purpose of the research is to identify the impact of various forms of application of information technologies for lifelong learning in order to provide the continuous self-development of each person without cultural or age restrictions and on the basis of rapid digital progress. A high level (96%) of need of the adult population in continuing education with the use of digital technologies has been established. The most effective ways to implement the concept of "lifelong learning" have been identified (educational camps, lifelong learning, mass open online courses, Makerspace activities, portfolio use, use of emoji, casual game, scientific research with iVR game, implementation of digital games, work in scientific cafes). 2 basic objectives of continuing professional education for adults have been outlined (continuous improvement of qualifications and obtaining new qualifications). The features of ICT application in adult education have been investigated by using the following methods, namely: flexibility in terms of easy access to ideas, solving various problems, orientation approach, functional learning, group or individual learning, integration of leisure, personal and professional activities, gamification. The advantages of application of information technologies for continuous education (economic, time, and adaptive) have been revealed. The concept of continuous adult learning in the context of digitalization has been concluded. The research provides a description of the structural principles of the concept of additional education; a system of information requests of the applicant, as well as basic technologies for lifelong learning. The research indicates the lack of comprehensive research in the relevant field. The practical significance of the research results lies in the possibility of using the obtained results for a wider acquaintance of the adult population with the importance of the application of lifelong learning for professional activities and the introduction of methods for its implementation in the educational policy of the state.

Continuous Ethanol Fermentation Using Membrane Cell Recycle Fermentor (Membrane Cell Recycle Fermentor에 의한 에탄올 연속 발효)

  • 김태석;이석훈;손석민;권윤중;변유량
    • Microbiology and Biotechnology Letters
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    • v.19 no.4
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    • pp.419-427
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    • 1991
  • Ethanol fermentation of glucose by a strain of Saccharomyces cereuisiae was studied in membrane recycle bioreactor, where the fermentation vessel was coupled with cross flow hollow fiber membrane. The cell recycle system controlled backflushing with fresh medium was proven to be effective in alleviating membrane fouling and allowing long term operation of high-cell continuous fermentation. Using 100 g/l initial glucose concentration, the maximum productivity of about 9 5 g/$l \cdot h$ has been achieved at dilution rate 2.5 $h^{-1}$ and bleed stream ratio 0.05 with the corresponding ethanol concentration of 35g/l and glucose conversion of 100%. Increasing the glucose concentration to 200 g/$l \cdot h$ resulted in an increase in ethanol concentration to 48 g/l and productivity to 120 g1l.h. Substrate conversion, however, was only 69%. This productivity was the highest value in the study, and about 38 fold more than that of batch culture and 17 fold more that of single stage continuous culture without cell recycling. No further increase in the productivity was obtained when the glucose concentration was increased reased to 300g/l.

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Color Removal of Culture Broth Containing Hyaluronic by Activated Carbon (활성탄을 이용한 히아루론산 배양액의 유색물질 제거)

  • 윤종원;김덕중
    • KSBB Journal
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    • v.8 no.2
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    • pp.143-149
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    • 1993
  • Activated carbons were used to examine their performance for the separation of undesirable colored materials from culture broth containing hyaluronic acid. Six local samples and a NORIT ROX 08 were tested, whereas the latter was mainly studied under batch and continuous modes. The optimal wavelength for the detection of colored materials was 330nm. The optimal choice of NORIT ROX 08 provided 30% colored residuals with 96% hyaluronic acid recovery of original broth in batch experiments. The nonlinear adsorption behavior of protein and colored materials with activated carbon (C) was correlated by a Langmuir equation to give 18C/24+C and 500C/892+C for protein and colored materials, respectively. It appears that colored materials were composed of 78% protein and 22% glucose residuals on the basis of clearance results. A microscopic study using a scanning electron microscope suggests that regeneration of used activated carbon with 0.1N NaOH and hot water was not satisfactory. The present study proposes that the continuous monitoring of colored materials during purification can be accomplished by Installation of a UV monitor commonly used for continuous detection of protein during the process, as resulted from the significant correlation of color (A330)=0.353protein(mg/ml)+0.1(R=99.7%).

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Kinetic Studies of Lactic Acid Fermentation(Part 4) -Kinetic Stuies on Continuous Cultivation- (유산균발효에 관한 동력학적연구(제4보) -연속배양에 있어서의 동력학적연구-)

  • LEE Keun-Tai;YANG Hyeun-Suk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.15 no.3
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    • pp.179-184
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    • 1982
  • The behavior of continuous flow culture of Lartobacillus bulgricus was investigated by application of Monod's kinetic model. The parameters obtained from Monod's chemostat theory successfully predicted the behavior of the chemostat. Then, it was found that Monod's kinetics were applicable to the growth rate dependence on glucose concentration. Under steady-state condition, the maximum growth rate, saturation constant, and wash out were found to be 0.62/hr, 7.69 g/1, 0.51/hr of continuous culture. And the optimum condition for the highest cell production was 0.41/hr in dilution rate, and at that point the cell production rate was 0.178g/1 hr.

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Continuous Ethanol Fermentation by Immobilized Kluyveromyces marxianus F043 Using Jerusalem Arichoke Powder (돼지감자 분말을 이용한 고정화 Kluyveromyces marxianus sp.의 에탄올 연속발효)

  • 신지현;최언호
    • Microbiology and Biotechnology Letters
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    • v.23 no.3
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    • pp.346-351
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    • 1995
  • To produce ethanol from Jerusalem artichoke powder efficiently, Kluyveromyces marxianus F043 cells were encapsulated in 2% sodium alginate and were cultured in a countinuous reactor to investigate the fermentation properties. Immobilized K. marxianus F043 cells were activated for 48 hours in a fermentor for continuous ethanol production. The culture in a CSTR using a Jerusalem artichoke substrate treated with 2% cellulase showed a decrease in ethanol concentration and an increase in residual saccharide concentration with a increasing dilution rate. Optimum conditions for high ethanol productivity and low residual saccharide output were clarified to be given at a dilution rate of 0.2 h$^{-1}$ and a Jerusalem artichoke medium concentration of 75 g/l. Ethanol productivity of 3.1 g/l-h and saccharide utilization of 62.6% were obtained under the optimum condition. When the fermentation was performed for 3 weeks under these conditions, the effluent medium showed stable ethanol concentrations of 16.3 - 17.9 g/l and viable cells of 6.60-7.16 log cells/ml without contamination. Trace amounts of methyl, n-propyl, iso-butyl, isoamyl alcohols besides ethanol were detected.

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