• Title/Summary/Keyword: Content of chlorophyll

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Toxicity evaluation based on particle size, contact angle and zeta potential of SiO2 and Al2O3 on the growth of green algae

  • Karunakaran, Gopalu;Suriyaprabha, Rangaraj;Rajendran, Venkatachalam;Kannan, Narayanasamy
    • Advances in nano research
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    • v.3 no.4
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    • pp.243-255
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    • 2015
  • In this investigation, ecotoxicity of nano and micro metal oxides, namely silica ($SiO_2$) and alumina ($Al_2O_3$), on the growth of green algae (Porphyridium aerugineum Geitler) is discussed. Effects of nano and micro particles on the growth, chlorophyll content and protein content of algae are analysed using standard protocols. Results indicate that $SiO_2$ nano and micro $SiO_2$ particles are non-toxic to P. aerugineum Geitler up to a concentration of 1000 mg/L. In addition, $Al_2O_3$ microparticles are less toxic to P. aerugineum Geitler, whereas $Al_2O_3$ nanoparticles are found to be highly toxic at 1000 mg/L. Moreover, $Al_2O_3$ nanoparticles decrease the growth, chlorophyll content, and protein content of tested algae. In addition, zeta potential and contact angle are also important in enhancing the toxicity of metal oxide nanoparticles in aquatic environment. This study highlights a new insight into toxicity evaluation of nanoparticles on beneficial aquatic organisms such as algae.

The Changes of Chlorophylls in Blanched and Fermented Chinese Cabbage (배추의 가열과 산 발효에 따른 Chlorophylls의 변화)

  • 김예숙;이혜수
    • Korean journal of food and cookery science
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    • v.1 no.1
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    • pp.27-32
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    • 1985
  • The changes of chlorophylls and pH of cooked water were investigated in blanched and fermented cabbage. In blanched cabbage, pheophytins were increased markedly with heating time. This was related to the changes of pH and the effects of heat. One minute blanching accelerated the formation of chlorophyllides by the activation of chlorophyllase. In fermented cabbage, pH and chlorophylls were decreased rapidly at the beginning of fermentation. At the final of fermentation, all chlorophylls and chlorophyllides were converted to pheophytins ana pheophorbides, and the content of pheophytins was higher than the content of pheophorbides. The content of pheophorbides in fermented cabbage was higher than that in blanched cabbage.

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Isolation of Chlorophyll Derivatives and $\beta$-Carotene from Mustard Leaf and Their Antioxidative Activities on the Lipid Autoxidation (갓에서 Chlorophyll 유도체 및 $\beta$-Carotene의 분리와 이들의 항산화 작용)

  • 송은승;전영수;최홍식
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.3
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    • pp.377-381
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    • 2001
  • Chlorophyll a and b, pheophytin a and b and $\beta$-carotene, crude chlorophylls and carotenoids (CCC) extracts of mustard leaf kimchi were isolated by DEAE-sepharose CL-6B and Sepharose CL-6B colume and TLC. The effects of chlorophyll a and b, pheophytin a and b and $\beta$-carotene on linoleic acid autoxidation were examined by the determination of peroxide value and conjugated dienoic acid content. Among them, chlorophyll a showed greater antioxidative activity than others, followed by chlorophyll b, pheophytin a, pheophytin b and $\beta$-carotene. Degradation of pheophytin b was observed to be slower than others and $\beta$-carotene showed highest degradation level during the autoxidation reaction of linoleic acid.

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Changes of Chlorophyll and their Derivative Contents during Storage of Chinese Cabbage, Leafy Radish and Leaf Mustard Kimchi (배추, 열무 및 갓김치 저장 중의 Chlorophyll 및 그 유도체의 함량변화)

  • 이종호;김경업;이용숙;김성희;정효숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.5
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    • pp.852-857
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    • 1998
  • Three kinds of kimchi using Chinese cabbage, leafy radish and mustard leaf were prepared by conventional method and stored at 5$^{\circ}C$ or 2$0^{\circ}C$ for 13 dyas. During storage at both temperatures, changes of the amounts of salt and ascorbic acid, pH and total acidity were determined, and the relationship of the decomposition of chlorophylls with the production of their derivaties was studied. At both storage temperatures, salt concentration of Chinese cabbage kimchi(3.7%), leafy radish kimchi(3.6%), mustard leaf kimchi(3.5%) was relatively constant during the entire storage period. However, pH and total acidity wre fluctuating with the remarkable changes during 3 days of storage. Ascorbic acid content was slowly decreased during the storage period and the decompositin rate of ascorbic and was greater at 2$0^{\circ}C$ than 5$^{\circ}C$. Among the kinds of kimchi tested, mustard leaf kimchi with the slow decomposition rate of ascorbic acid contained relatively high ascorbic acid content, while leafy radish kimchi contained the lowest content. At both storage temperatures, the production of pheophytin and pheophorbide from decomposition of chlorophyll was least in mustard leaf kimchi, but similar production rates in leafy radish and Chinese cabbage kimchi were observed.

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Effect of Addition of Egg Yolk Lecithin on the Lipid Oxidation of a Water/canola Oil Emulsion (달걀 노른자위 레시틴의 첨가가 물/카놀라 기름 에멀션의 지방질 산화에 미치는 영향)

  • Choe, Jeesu;Choe, Eunok
    • Korean Journal of Food Science and Technology
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    • v.47 no.5
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    • pp.561-566
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    • 2015
  • Effect of the addition of egg yolk lecithin at a concentration of 350 mg/kg on iron-catalyzed autoxidation and chlorophyll-photosensitized oxidation of a water/canola oil emulsion (W/O) during storage at $25^{\circ}C$ was studied based on headspace oxygen consumption and hydroperoxide production. Changes in the phospholipid (PL) composition of the emulsion were determined by high performance liquid chromatography. Headspace oxygen consumption and hydroperoxide content of the emulsion increased with storage time, and addition of egg yolk lecithin did not have any significant effect on these parameters during iron-catalyzed autoxidation and chlorophyll-photosensitized oxidation of the emulsion. PL content of the emulsion decreased during both oxidations, and the degradation rate was higher during autoxidation than during photosensitized oxidation. Phosphatidylcholine content ratio tended to increase during autoxidation. The results suggest that egg yolk lecithin in canola oil emulsion behaves differently during iron-catalyzed autoxidation and chlorophyll-photosensitized oxidation.

Growth Responses of Petunia as Affected by Several Pollutants in Vitro (Petunia 조직배양시 몇가지 공해물질이 생장반응에 미치는 영향)

  • 백기엽;최성렬;이재욱;최주견
    • Journal of Plant Biology
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    • v.27 no.3
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    • pp.139-147
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    • 1984
  • This in vitro study was employed to clarify the effects of several pollutants i.e. $SO_2$, fluoride, cadmium(Cd), aluminum(Al) and NaCl, on the organogenesis and growth responses of shoot-tip, stem and multiple-buds segments derived from hypocotyl or cotyledon culture of petunia seedlings. ${Na_2}{SO_3}$levels of more than 200$\mu{g}$/ml had significantly reduced organogenesis, growth, and chlorophyll content. The injuries caused by ${Na_2}{SO_3}$, concentration of more than 400$\mu{g}$/ml were alleviated by increasing hydrogenion concentration of medium, indicating some relationship between two factors. Organogenesis was not affected by the fluoride concentration up to 100ppm in the media, but the growth and chlorophyll content were greatly reduced by the fluoride. The effect of Cd depended on the explant sources used for the culture; 1.0ppm was effective for fresh weight increase in shoot tip culture, and 3.0ppm in stem segments culture. Organogenesis and growth were greatly reduced by more than 10.0 Cd treatment. Growth and formation of shoots were better with Na conc. of 0.3% compared to control, but those of roots were inhibited. Na concentration goes over 1.0%, organogenesis and subsequent growth were inhibited, and chlorophyll synthesis was drastically reduced. Chlorophyll content was increased on the medium supplemented with Al 50$\mu{g}$/ml compared to control. However the formation and growth of shoots were greatly inhibited with more than 400$\mu{g}$/ml and roots were not produced at all.

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Evaluation of Chlorophyll Content, Antioxidant Activity and Phenolic Compounds in the Seedlings of Rice-type Tartary Buckwheat

  • Sharma, Pankaja;Lee, Kooyeon;Park, Cheol Ho
    • Korean Journal of Plant Resources
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    • v.27 no.6
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    • pp.629-634
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    • 2014
  • Rice type tartary buckwheat is used as a substitute for rice in many Asian countries due to its easy dehulling character. The objective of the present study was to determine the chlorophyll, total polyphenol (TP), total flavonoid (TF), antioxidant activity and to quantify the bioactive compounds rutin, quercetin and chlorogenic acid in the seedlings of rice-type tartary buckwheat (RTTB). Young seedlings exhibited higher antioxidant (DPPH radical inhibition) activity in dose dependent manner. TP and TF content were highest ($3017.46{\pm}201.84{\mu}g$ TAE/100 mg dw and $1916.0{\pm}102.95{\mu}g$ QE/100 mg dw respectively) in 3 days after germination (DAG) seedlings compare to 6 and 9 DAG. The contents of rutin and quercetin increased with growing stage of seedlings. However, the chlorogenic acid decreased with increasing growth. Overall, RTTB seedlings can be regarded as a strong source of phenolics and have high possibility for food and nutraceutical application due to their efficient antioxidant properties, higher chlorophyll and phytochemical content.

Characterization of Chlorella vulgaris Mutants Producing High Chlorophyll (클로로필 고생산성 Chlorella vulgaris 변이주의 특성 분석)

  • Park, Hyun-Jin;Kim, Ok Ju;Ha, Ji Min;Choi, Tae O;Lee, Jae-Hwa
    • Microbiology and Biotechnology Letters
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    • v.43 no.3
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    • pp.275-279
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    • 2015
  • Micro-algae are unicellular photosynthetic organisms and produce pigments such as chlorophyll and carotenoid. Chlorella contains a lot of protein and functional components like lipids, chlorophyll and carotenoids. In this study we induced mutants of Chlorella vulgaris (C. vulgaris) through ultraviolet radiation (UV-B) and selected two mutants by pigment (chlorophyll and carotenoids) content. We named the mutants ‘UBM1-2’, ‘UBM2-57’ and they were cultivated for 21-days. Cell growth, dry cell weight, protein content, lipid and pigments content were measured. The results indicated that the mutants displayed slower cell growth, lower dry cell weight and protein content than the wild type. However, for UBM1-2 the lipid content was 21% higher than the wild type. In addition, the mutants’ chlorophyll content was 37% and 89% higher than the wild type and the carotenoids content was 27% and 70% higher than the wild type, respectively.

Comparison of Non-destructive Measuring Methods of Tomato Plant to Detect N, P and Ca Deficient Stresses (토마토 잎의 비파괴 계측에 의한 N, P, Ca 결핍 장해 진단법 비교)

  • 서상룡;류육성;정갑채;성제훈;이성희
    • Journal of Biosystems Engineering
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    • v.25 no.6
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    • pp.517-526
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    • 2000
  • A series of experiments was conducted to evaluate the capability of detecting nutrimental deficient stress of N, P and Ca of a tomato plant using several fast and intact type physiological properties measuring devices - a chlorophyll content meter an infra-red thermometer to measure leaf temperature a chlorophyll fluorescence meter a porometer an optical spectrometer a multi-scan radiometer and a canopy analyzer. to detect N deficient stress a chlorophyll content meter a spectrometer and a multi-scan radiometer were useful and their possibility to detect was estimated as about 50%, 100% and 100% respectively. To detect P deficient stress the infra-red thermometer the porometer and the spectrometer proved their usefulness an their possibility to detect was estimated as about 70%, 70% and 70% respectively. To detect Ca deficient stress an thermometer a porometer a spectrometer and a multi-scan radiometer were useful and their possibility to detect was estimated as about 60%, 70%,80% and 100% respectively. The experiments resulted that use of a spectrometer and a multi-scan radiometer in combination with a chlorophyll content meter an infra-red thermometer and a porometer were desirable to distinguish the nutrimental stress tested in the study.

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