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Effect of SPR Chip with Nano-structured Surface on Sensitivity in SPR Sensor (나노형상을 가진 표면플라즈몬공명 센서칩의 감도 개선 효과)

  • Cho, Yong-Jin;Kim, Chul-Jin;Kim, Namsoo;Kim, Chong-Tai;Kim, Tae-Eun;Kim, Hyo-Sop;Kim, Jae-Ho
    • Food Engineering Progress
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    • v.14 no.1
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    • pp.49-53
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    • 2010
  • Surface plasmon resonance (SPR) which is utilized in thin film refractometry-based sensors has been concerned on measurement of physical, chemical and biological quantities because of its high sensitivity and label-free feature. In this paper, an application of SPR to detection of alcohol content in wine and liquor was investigated. The result showed that SPR sensor had high potential to evaluate alcohol content. Nevertheless, food industry may need SPR sensor with higher sensitivity. Herein, we introduced a nano-technique into fabrication of SPR chip to enhance SPR sensitivity. Using Langmuir-Blodgett (LB) method, gold film with nano-structured surface was devised. In order to make a new SPR chip, firstly, a single layer of nano-scaled silica particles adhered to plain surface of gold film. Thereafter, gold was deposited on the template by an e-beam evaporator. Finally, the nano-structured surface with basin-like shape was obtained after removing the silica particles by sonication. In this study, two types of silica particles, or 130 nm and 300 nm, were used as template beads and sensitivity of the new SPR chip was tested with ethanol solution, respectively. Applying the new developed SPR sensor to a model food of alcoholic beverage, the sensitivity showed improvement of 95% over the conventional one.

Dietary phosphorus deficiency impaired growth, intestinal digestion and absorption function of meat ducks

  • Xu, Huimin;Dai, Shujun;Zhang, Keying;Ding, Xuemei;Bai, Shiping;Wang, Jianping;Peng, Huanwei;Zeng, Qiufeng
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.12
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    • pp.1897-1906
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    • 2019
  • Objective: An experiment was conducted to investigate the effects of dietary non-phytate phosphorus (nPP) deficiency on intestinal pH value, digestive enzyme activity, morphology, nutrient utilization, and gene expression of NaPi-IIb in meat ducks from 1 to 21 d of age. Methods: A total of 525 one-d-old Cherry Valley ducklings were fed diets (with 7 pens of 15 ducklings, or 105 total ducklings, on each diet) with five levels of nPP (0.22%, 0.34%, 0.40%, 0.46%, or 0.58%) for 21 d in a completely randomized design. Five experimental diets contained a constant calcium (Ca) content of approximately 0.9%. Body weight (BW), body weight gain (BWG), feed intake (FI), and feed to gain ratio (F:G) were measured at 14 and 21 d of age. Ducks were sampled for duodenum and jejunum digestion and absorption function on 14 and 21 d. Nutrient utilization was assessed using 25- to 27-d-old ducks. Results: The results showed ducks fed 0.22% nPP had lower (p<0.05) growth performance and nutrient utilization and higher (p<0.05) serum Ca content and alkaline phosphatase (ALP) activity. When dietary nPP levels were increased, BW (d 14 and 21), BWG and FI (all intervals), and the serum phosphorus (P) content linearly and quadratically increased (p<0.05); and the jejunal pH value (d 14), duodenal muscle layer thickness (d 14), excreta dry matter, crude protein, energy, Ca and total P utilization linearly increased (p<0.05); however, the serum ALP activity, jejunal $Na^+-K^+$-ATPase activity, and duodenal NaPi-IIb mRNA level (d 21) linearly decreased (p<0.05). Conclusion: The results indicated that ducks aged from 1 to 21 d fed diets with 0.22% nPP had poor growth performance related to poor intestinal digestion and absorption ability; but when fed diets with 0.40%, 0.46%, and 0.58% nPP, ducks presented a better growth performance, intestinal digestion and absorption function.

Antioxidant activity of partially characterized polysaccharides from the edible mushroom Pleurotus djamor var. roseus

  • Raman, Jegadeesh;Sivakumar, Archana;Lakshmanan, Hariprasath;Raaman, Nanjian;Shin, Hyun-Jae
    • Journal of Mushroom
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    • v.19 no.3
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    • pp.140-149
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    • 2021
  • Mushroom-derived polysaccharides, which are the primary bioactive constituents, are beneficial for human health. Polysaccharides have immuno-modulation, antitumor, and antioxidant properties. Additionally, they have antiviral properties and protect against chronic radiation stress. In this study, high yield water-soluble polysaccharides were obtained from Pleurotus djamor var. roseus basidiocarps. The crude polysaccharide (CP) was extracted sequentially by hot water and ethanol precipitation. The yield of the brown CPs was 5.6% dw. Diethylaminoethyl cellulose and Sepharose-6B column chromatography of CPs generated several fractions. Total glucan content was determined in all the fractions. The F1 fraction displayed the highest sugar content and was considered as a purified polysaccharide (PP). The total glucan and β-glucan content in the four fractions ranged between 76.85-2.95% and 75.08-1.46%, respectively. The yield of the PPs was 300 mg, and it was obtained as a white powder. The PPs were characterized by Fourier-transform infrared spectroscopy (FTIR) and thin-layer chromatography. The FTIR spectral details confirmed the presence of a xylopentose polysaccharide. The antioxidant activity of the PPs was evaluated using in vitro 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay and superoxide radical scavenging assay. The PPs showed strong DPPH free radical and superoxide anion radical scavenging activities in a dose-dependent manner. Purified PPs free of phenolics, protein, and carbohydrates were mainly responsible for the radical scavenging activity. The data suggest the potential of PPs as natural antioxidants.

Suggestion and Evaluation for Prediction Method of Landslide Occurrence using SWAT Model and Climate Change Data: Case Study of Jungsan-ri Region in Mt. Jiri National Park (SWAT model과 기후변화 자료를 이용한 산사태 예측 기법 제안과 평가: 지리산 국립공원 중산리 일대 사례연구)

  • Kim, Jisu;Kim, Minseok;Cho, Youngchan;Oh, Hyunjoo;Lee, Choonoh
    • Journal of Soil and Groundwater Environment
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    • v.26 no.6
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    • pp.106-117
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    • 2021
  • The purpose of this study is prediction of landslide occurrence reflecting the subsurface flow characteristics within the soil layer in the future due to climate change in a large scale watershed. To do this, we considered the infinite slope stability theory to evaluate the landslide occurrence with predicted soil moisture content by SWAT model based on monitored data (rainfall-soil moisture-discharge). The correlation between the SWAT model and the monitoring data was performed using the coefficient of determination (R2) and the model's efficiency index (Nash and Sutcliffe model efficiency; NSE) and, an accuracy analysis of landslide prediction was performed using auROC (area under Receiver Operating Curve) analysis. In results comparing with the calculated discharge-soil moisture content by SWAT model vs. actual observation data, R2 was 0.9 and NSE was 0.91 in discharge and, R2 was 0.7 and NSE was 0.79 in soil moisture, respectively. As a result of performing infinite slope stability analysis in the area where landslides occurred in the past based on simulated data (SWAT analysis result of 0.7~0.8), AuROC showed 0.98, indicating that the suggested prediction method was resonable. Based on this, as a result of predicting the characteristics of landslide occurrence by 2050 using climate change scenario (RCP 8.5) data, it was calculated that four landslides could occur with a soil moisture content of more than 75% and rainfall over 250 mm/day during simulation. Although this study needs to be evaluated in various regions because of a case study, it was possible to determine the possibility of prediction through modeling of subsurface flow mechanism, one of the most important attributes in landslide occurrence.

Preliminary Research of the Sedimentary Environment in Bupyeng Reservoir Region, Soyang Lake in Chuncheon - Focus on Sentinel-2 Satellite Images and in-situ data - (춘천시 소양호 상류 부평지구의 퇴적환경에 대한 선행연구 - 현장조사와 위성영상자료를 중심으로 -)

  • Kim, GeonYoung;Kim, Dain;Kim, TaeHun;Lee, JinHo;Jang, YoSep;Choi, HyunJin;Shim, WonJae;Park, SungJae;Lee, Chang-Wook
    • Korean Journal of Remote Sensing
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    • v.34 no.6_1
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    • pp.1119-1130
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    • 2018
  • Soyang Lake has been contributing to economic growth by preventing flood damage in the metropolitan area, the water level in the middle to upper flow of lake has been greatly decreased due to the drought in 2015. In order to restore the existing flow rate, Bupyungbo has been built in Bupyeong-ri, Shin Nam-myeon, Inje-gun to cause artificial changes on the sedimentary environment of Bupyeong freshwater region. Therefore, this study intends to confirm the changes of sedimentary environment since Bupyeongbo has been utilized. For this study, we used the Sentinel-2 satellite image data periodically to measure the dimension of water according to the volume of water kept near Bupyung district and analyzed the particle size and the percentage of water content of the sediments through field study. The Sentnel-2 satellite images showed us how the water surface has been changed and that during the period from September 2017 to October 2018, the minimum and maximum area of water surface was observed in June 2018 and in January 2018, respectively. In addition, we find that the smaller being the particle size, the higher having the water content and that there is higher the correlation between the water content and the grain size of the sediment layer. Hereafter, if we will acquire the drone images at Bupyung district, we expect that we will be able to measure the distribution of sediments in the same area according to different time periods and observe various kinds of sediment through field work.

Preparation and Characterization of Polysulfone Membranes Using PVP as an Additive (폴리비닐피롤리돈 첨가제를 이용한 폴리설폰막의 제조 및 특성 분석)

  • Lee, Jin Young;Lee, Kune Woo;Han, Myeong-Jin;Park, So-Jin
    • Applied Chemistry for Engineering
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    • v.22 no.3
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    • pp.277-285
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    • 2011
  • Polysulfone (PSf) membranes were prepared via the phase inversion process. Polyvinylpyrrolidone (PVP) was added as a nonsolvent additive in the casting solution containing a mixture of PSf and n-methylpyrrolidone. The added PVP played a role of enhancing liquid-liquid phase separation of the casting solution, and significantly reduced the solution fluidity. When prepared via the diffusion-induced process using water as a precipitation nonsolvent, the solidified membranes revealed a typical asymmetric structure irrespective of the addition of PVP. With 5 wt% PVP content, the finger-like cavities were more developed in the membrane sublayer compared to that of the membranes prepared without PVP. In contrast, with more than 10 wt% of PVP, the formation of finger-like cavities was suppressed, and the thickness of polymer nodule layer was increased. The surface porosity was also increased with the PSf content in the casting solution. The water permeability curve as a function of PVP addition revealed the inflection point. The maximum water permeability for 12 wt% PSf membrane was obtained with 5 wt% PVP content, and that for 18 wt% PSf membrane with 15 wt% PVP.

Physical Properties of Major Bedrocks in Chungju-Goesan Area as Aggregates (충주-괴산일대에서 산출되는 주요 기반암의 골재로서의 물성특징)

  • Byoung-Woon You;Jaehyung Yu
    • Economic and Environmental Geology
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    • v.55 no.6
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    • pp.649-659
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    • 2022
  • This study examined the granite, quartzite, phyllite, schist, and gneiss as aggregate resources among the original rock distributed in the Chungju-Goesan area. The granite distributed in the study area is mainly composed of Jurassic biotite granite, and the quartzite layer is from the Daehyangsan quartzite Formation distributed on the upper part of the Gyemyeongsan Formation and the Hyangsan-ri dolomitic limestone Formation. In addition, phyllite is pophyrytic phyllite-schist from the Hwanggangri Formation of the Okcheon group, schist is chlorite schist, from the Munjuri Formation of the Okcheon group, and gneiss is porphyroblastic gneiss which is the upper part of the Seochangri Formation. Aggregate quality evaluation factors of these rocks included fineness modulus, absorption, unit weight, absolute dry density, solid content, porosity, resistance to abrasion, and soundness. In the case of granite, it was found to be partially unsatisfactory in terms of unit weight, solid content, porosity, and resistance to abrasion. Gneiss was found to be out of the standard values in resistance to abrasion and schist in porosity and solid content. As for the overall quality of aggregate resources, it was analyzed that quartzite, gneiss, and phyllite showed excellent quality. Aggregate quality tests are performed simply for each rock, but the rock may vary depending on the morphology of the mineral. Therefore, when analyzing and utilizing the quality evaluation of aggregate resources, it will be possible to use them more efficiently if the rock-mineralological research is performed together.

The Improvement Effect of Pinus densiflora Forest Disturbed by Human Trampling in the Solbat Neighborhood Park, Gangbuk-gu, Seoul (서울시 강북구 솔밭근린공원 소나무림 답압 피해 개선사업 효과 연구)

  • Kwon, Ki-Young;Han, Bong-Ho;Park, Seok-Cheol;Choi, Jin-Woo
    • Journal of the Korean Institute of Landscape Architecture
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    • v.40 no.5
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    • pp.148-159
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    • 2012
  • The purpose of this study is to validate the effect of improvement such measures as fence installation or planting of bush and herbaceous plants taken from Pinus densiflora forest in Solbat Neighborhood Park in Seoul, which was damaged by stamping. The study was conducted in 2005 and 2010 in order to analyze changes in planting types, planting structure of Pinus densiflora forest, soil hardness, cross-sectional structure of soil, and physicochemical characteristics of soil. It was also measured by the growth of the branches and the diameter of Pinus densiflora, comparing before and after the improvement to study the effect of restoring Pinus densiflora forest damaged by stamping. When it comes to a change in planting type, Pinus densiflora forest without underlay was reduced from 48.5% in 2005 to 6.8% in 2010. Pinus densiflora forest with bush and herbaceous plants was increased dramatically from 7.4% to 46.8%. Regarding planting structure, in most area of the subject site, Pinus densiflora forest without under layer was transformed into the one with bush and herbaceous plants including Rhododendron mucronulatum, Rhododendron schippenbachii, Hemerocallis fulva, Aceriphyllum rossii, Hosta plantaginea growing in a wide area. The soil in the Solbat Neighborhood Park was very stiff with soil hardness of $54.8kg/cm^2$ in average. After the improvement efforts made in the Park in 2010, the soil hardness was mostly less than $4kg/cm^2$, being in a good condition with little influence on the growth of plants. When it comes to the cross-sectional structure of soil, litter layer didn't exist in 2005 because of stamping and the organic matter layer was only 1.0cm thick, which provided an unfavorable condition for plant growth. However, after improvement, litter layer was formed up to 3.0cm and thickness of the organic matter layer also went up to 1.5~8.0cm in 2010 because the damage from stamping was reduced. Concerning the physicochemical characteristic of soil, in 2005 soil showed pH 5.76~6.70, organic matter content 7.15~10.55%, and available phosphorus 9.38~26.47mg/kg, having no big problems as a soil environment for growth of Pinus densiflora. 15 trees of Pinus densiflora were selected to see branch growth and it was found that the branches tended to grow better after improvement. 70 trees of Pinus densiflora from various grades of soil hardness also were selected to identify changes of diameter growth. In most cases, it was analyzed that Pinus densiflora grew better after improvement. After conducting this study, it was validated that such measures as fence installation or planting of bush and herbaceous plants to restore Pinus densiflora Forest damaged by stamping were effective in improving growth of Pinus densiflora.

Spatial Distribution of Transparent Exopolymer Particles(TEP) and Their Relation to Carbon Species in the Euphotic Layer of the Northern East Sea (동해 북부해역 유광층에서 TEP 분포와 이산화탄소 인자와의 상호관련성)

  • Jeon, Hyun-Duck;Rho, Tae-Keun;Lee, Tong-Sup
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.17 no.2
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    • pp.33-44
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    • 2012
  • Transparent exopolymer particles (TEP) are formed by aggregation of polysaccharide products excreted by phytoplankton and have sticky character like gel. They play important role in the production of marine snow in water column. To study the distribution pattern of TEP concentration and its role in carbon cycle in the surface ocean, we measured pH, Total alkalinity (TA), and chlorophyll-a in addition to physical characteristics of seawater within the surface water column. TEP concentrations ranged from nearly undetectable values to $338{\mu}g\;Xeq\;l^{-1}$. They were considerably lower than previously reported values from costal sites, but showed similar values observed in other oceanic region during phytoplankton bloom periods. The spatial distribution of TEP concentrations were similar to those of chlorophyll-a, which indicate that the production of TEP were closely related to phytoplankton. Calculated total dissolved inorganic carbon ($TCO_2$) from the pH and TA was normalized to 35 psu of salinity ($NTCO_2$) and showed negative linear relationship with temperature. Biological drawdown of $NTCO_2$ ($NTCO_{2bio}$) was estimated from the difference between theoretical $NTCO_2$ values and observed $NTCO_2$. In the warm region located south of $40^{\circ}N$ along the $132.5^{\circ}N$ meridional lines, $NTCO_{2bio}$ showed negative value and TEP concentrations were high. This suggested that negative $NTCO_{2bio}$ may be attributed to the biological processes. At the stations located between 44 and $46^{\circ}N$, TEP concentrations showed high concentration at the chlorophyll-a maximum layer within the water column while they showed low concentration in the surface layer. Carbon content of TEP constituted about 40% of $NTCO_{2bio}$ at the chlorophylla maximum layer. In this study, we could not observe any positive and negative relationship between TEP concentration and $NTCO_2$ or pH. It is obvious that we should consider the importance of TEP in the biological carbon cycling processes within surface layer.

A Study on Effects of Air-delivery Rate upon Drying Rough Rice with Unheated Air. (벼의 자연통풍건조에 있어서 통풍량이 건조에 미치는 영향에 관한 연구)

  • 이상우;정창주
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.16 no.1
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    • pp.3293-3301
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    • 1974
  • An experimental work was conducted by using a laboratory-made model dryer to investigate the effect of the rate of natural forced-air on the drying rate of rough rice which was deposited in the deep-bed. The dryer consisted of 8 cylinderical containers with grain holding screen at their bottoms, each of which having 30cm in diameter and 15cm in height. The containers were sacked vertically with keeping them air-tight by using paper tape during dryer operation. Two separate layers of containers were operated in the same time to have two replications. The moisture contents of grains within each bins after predetermined period of dryer operation were determined indirectly by measuring the weight of the individual containers. The air-rates were maintained at 6 levels, or 5, 8, 10, 15, 18 and 20 millimenters of static head of water. The roomair conditions during dryer operation were maintained in the range of 10-l5$^{\circ}C$ in temperature and 40-60% in relative humidity. The results of the study are summarized as follows: 1. Drying characteristics of the grains in the bottom layers were approximately the same regardless of airdelivery rates, giving the average drying rate as about 0.35 percent per hour after 40-hour drying period, during which moisture content (w. b.) reduced from 24 percent to about 10 percent. 2. After about 40-hour drying period, the mean drying rates increased from 0.163 percent per hour to 0.263 percent per hour as air-flow rates increased from 5mm to 87.16mm of static head of water. In the same time, the moisture differences of grains between lower and upper layers varied from 12.7 percent at the air rate of 5mm of water head to 7.5 percent at the air-flow rate of 20mn of water head. Thus, the greater the air-flow rate was, the more overall improvement in drying performance was. Additionally, from the result of ineffectiveness of drying grain positioned at 70cm depth or above by the air rate of 5mm of static head of water it may be suggested in practical application that the height of grain deposit would be maintained adequately within the limits of air-rates that may be actually delivered. 3. Drying after layer-turning operation was continued for about 30 hours to test the effectiveness of reducing moisture differences in the thick layers. As a result of this layer-turning operation, moisture distribution through layers approached to narrow ranges, giving the moisture range as about 7 percent at air-flow rate of 5mm head of water, about 3 percent at 10mm head about 2 percent at 15mm head, and less than 1 percent at 20mm head. In addition, from the desirable results that drying rate was rapid in the lower layers and dully in the upper layers, layer-turning operation may be very effective in natural air drying with deep-layer grain deposit, especially when the forced air was kept in low rate. 4. Even though the high rate of air delivery is very desirable for deep-layer natural-air drying of rough rice, it can be happened that the required air delivery rate could not be attained because of limitation of power source available on farms. To give a guide line for the practical application, the power required to perform the drying with the specified air rate was analyzed for different sizes of drying bin and is given in Table (5). If a farmer selects a motor of which size is 1 or {{{{1 { 1} over {2 } }}}} H.P. and air-delivery rate which ranges from 8~10mm of head, the diameter of grain bin may be suggested to choose about 2.4m, also power tiller or other moderate size of prime motor may be recommended when the diameter of grain bin is about 5.0m or more for about 120cm grain deposit.

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