• Title/Summary/Keyword: Content element analysis

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A Commensal Thermophile, Symbiobacterium toebii: Distribution, Characterization, and Genome Analysis

  • Bae Jin-Woo;Kim Kwang;Song Jae Jun;Ha Jae Seok;Kim Joong-Jae;Kang Gwan-Tae;Kim Mi-Hwa;Hong Seung-Pyo;Sung Moon-Hee
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2001.11a
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    • pp.46-53
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    • 2001
  • A commensal thermophile, Symbiobacterium toebii, isolated from hay compost (toebii) in Korea commensally interacted with a thermophilic Geobacillus toebii sp. nov., which was a new species within the genus Geobacillus on the basis of the phenotypic traits and molecular systematic data. S. toebii required the crude extracts and/or culture supernatant of the Geobacillus toebii for axenic growth and could grow on the temperature between 45 and $70^{\circ}C$ (optimum: $60^{\circ}C$; 2.4 h doubling time) and pH 6.0 and 9.0 (optimum: pH 7.5). The G+C content of the genomic DNA was $65 mol\%$, and the major quinones were MK-6 and MK-7. A phylogenetic analysis of its 16S rDNA sequence indicated that Symbiobacterium toebii was closely related with solely reported Symbiobacterium thermophilum. The presence of the commensal thermophile 16S rDNA and accumulation of indole in all the enriched cultures indicate that Symbiobacterium toebii is widely distributed in the various soils. The genome of S. toebii constituted a circular chromosome of 3,280,275 base pairs and there was not an extra-chromosomal element (ECE). It contained about 4,107 predicted coding sequences. Of these protein coding genes, about $45.6\%$ was encoded well-known proteins and annotated the functional assignment of 1,874 open reading frames (ORFs), and the rest predicted to have unknown functions. The genes encoding thermostable tyrosine phenol-lyase and tryptophan indole-lyase were cloned from the genomic DNA of S. toebii and the enzymatic production of L-tyrosine and L-tryptophan was carried out with two thermostable enzymes overexpressed in recombinant E. coli.

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Numerical Analysis of the Change in Groundwater System with Tunnel Excavation in Discontinuous Rock Mass (불연속 암반에서의 터널굴착에 따른 지하수체계 변화에 대한 수치해석적 연구)

  • Park, Jung-Wook;Son, Bong-Ki;Lee, Chung-In;Song, Jae-Joon
    • Tunnel and Underground Space
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    • v.18 no.1
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    • pp.44-57
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    • 2008
  • In this study, a 2D finite-element analysis, using the SEEP/W program, was carried out to estimate the amount of groundwater flawing into a tunnel, as well as the groundwater tables around wetland areas during and after a tunnel excavation through rock mass. Four sites along the Wonhyo-tunnel in Cheonseong Mountain (Gyeongnam, Korea) were analysed, where the model damain of the tunnel included both wetland and fault zone. The anisotropy of the hydraulic conductivities of the rock mass was calculated using the DFN model, and then used as an input parameter for the cantinuum model. Parametric study on the influencing factors was perofrmed to minimize uncertainties in the hydraulic properties. Moreover, the volumetric water content and hydraulic conductivity functions were applied ta the model to reflect the ability of a medium ta store and transport water under both saturated and unsaturated conditions. The conductivity of fault zone was assumed ta be $10^{-5}m/sec\;or\;10^{-6}m/sec$ and the conductivity of grouting zone was assumed as 1/10, 1/50 or 1/100 of the conductivity of rock mass. Totally $6{\sim}8$ cases of transient flow simulation were peformed at each site. The hydraulic conductivities of fault zone showed a significant influence on groundwater inflow when the fault zone crossed the tunnel. Also, groundwater table around wetland maintained in case that the hydraulic conductivity of grouting zone was reduced ta be less than 1/50 of the hydraulic conductivity of rock mass.

Game Analysis for Next Generation User Interface based on Storytelling (차세대 스토리텔링기반 유저 인터페이스를 위한 게임분석)

  • Lee, Dae-Young;Kim, Seon-Ju;Yu, Hui-Beom;Won, Sun-Jin;Sung, Jung-Hwan
    • 한국HCI학회:학술대회논문집
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    • 2008.02b
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    • pp.534-539
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    • 2008
  • Nowadays, UI(User Interface)'s main target area is getting larger from user's convenience oriented interface, to user's satisfaction oriented interface. In this study, we attempted 'Storytelling User Interface' for fulfillment of user's satisfaction. We analyzed UI in games, distinguished and clarified its effectiveness that conveys main contents and special features to its audience. The most important element in storytelling UI is 'Inherent Drama'. This was proved through the game analysis with Blizzard's 'Diablo', Sierra's 'Home world', Eden Games' 'Test Drive Unlimited', EA's 'Black And White', and Sony Entertainment's 'Eye Of Judgment'. And we divided storytelling elements into 3 pieces, Mood Design - that shows story's background, or traits, Natural Control - for user's feeling as a hero in the content, and Flow Directing - using animation, visual design and sound, etc. Via these elements, we can make the best use of background, icon, animation, every structures and flow in games. Finally, we embodied three pieces for putting in practice for effective improving interface.

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Analysis of Science Educational Contents of Singapore, Canada and US Focused on the Integrated Concepts (통합개념을 중심으로 한 싱가포르, 캐나다와 미국의 과학교육과정 내용 요소 분석)

  • Lee, Yoonha;Yoon, Heojeong;Song, Joo-Yeon;Bang, Dami
    • Journal of The Korean Association For Science Education
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    • v.34 no.1
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    • pp.21-32
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    • 2014
  • The feasibility of integrated concepts as a key element in designing integrated science curriculum has been investigated by analysing science contents included in performance expectations stated at different grades. The science curriculum of Singapore and the state of Ontario in Canada, and next generation of science standard (NGSS) were selected. Each of them presents theme, fundamental concepts, and crosscutting concepts, which has the characteristics of integrated concepts proposed in the study. Analysis showed that theme, fundamental concepts, and crosscutting concepts were influenced by the characteristics of each curriculum. In addition, science contents related to integrated concepts at different grades varied with the nature of integrated concepts. Based on results, some suggestions were made. First, the total number of integrated concepts should be considered for designing integrated curriculum. Second, the nature of integrated concepts and science contents associated with the integrated concepts should be considered. The integrated concepts should be vast and deep enough in the meaning to contain various content knowledge of different science domains. Third, it should be considered that how the integrated concepts have to be presented at different grades.

Analysis of Compositions for Effective Utilization of Fish Scales (생선비늘의 유효이용을 위한 성분분석)

  • 전유진;김용태;김세권
    • Journal of Life Science
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    • v.8 no.5
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    • pp.589-597
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    • 1998
  • In order to effectively utilize marine processing by-product such as fish scale, chemical compositions for the scale were analyzed. The selected fishes were gray mullet, Mugil cephalus, living in the sea and carp, Cyprinus carpio in the fresh water, having a lot of scales among the fishes living in seawater and fresh water. And we also investigated the difference in the chemical compositions between gray mullet and carp, depending on both living circumstances. The major components of the scales were found to be crude ash and crude protein which were each about 49% for gray mullet and which were about 20% and 79% for carp, respectively, on the basis of dried scales. The proteins extracted from both scales proved to be collagen through amino acid compositions and SDS polyacrylamide gel electrophoretic patterm. Also this scale collagen was assumed to by Type I collagen because the migration rate of $\alpha$1 and $\alpha$2 subunit of the collagen were almost the same those as calf skin Type I collagen. Most of proteins from gray mullet was collagen, however, the collagen content in proteins from carp was estimated to be only about 53%, on the basis of the ratio of hydroxyproline to protein. The crude ashes of both scales identified to be hydroxyapatite through element compositions and X-ray diffraction analysis. In conclusion, both fishes in different living circumstances were almost similar to in the chemical compositions but chemical contents for crude ash and crude protein.

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Prediction from Linear Regression Equation for Nitrogen Content Measurement in Bentgrasses leaves Using Near Infrared Reflectance Spectroscopy (근적외선 분광분석기를 이용한 잔디 생체잎의 질소 함량 측정을 위한 검량식 개발)

  • Cha, Jung-Hoon;Kim, Kyung-Duck;Park, Dae-Sup
    • Asian Journal of Turfgrass Science
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    • v.23 no.1
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    • pp.77-90
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    • 2009
  • Near Infrared Reflectance Spectroscopy(NIRS) is a quick, accurate, and non-destructive method to measure multiple nutrient components in plant leaves. This study was to acquire a liner regression equation by evaluating the nutrient contents of 'CY2' creeping bentgrass rapidly and accurately using NIRS. In particular, nitrogen fertility is a primary element to keep maintaining good quality of turfgrass. Nitrogen, moisture, carbohydrate, and starch were assessed and analyzed from 'CY2' creeping bentgrass clippings. A linear regression equation was obtained from accessing NIRS values from NIR spectrophotometer(NIR system, Model XDS, XM-1100 series, FOSS, Sweden) programmed with WinISI III project manager v1.50e and ISIscan(R) (Infrasoft International) and calibrated with laboratory values via chemical analysis from an authorized institute. The equation was formulated as MPLS(modified partial least squares) analyzing laboratory values and mathematically pre-treated spectra. The accuracy of the acquired equation was confirmed with SEP(standard error of prediction), which indicated as correlation coefficient($r^2$) and prediction error of sample unacquainted, followed by the verification of model equation of real values and these monitoring results. As results of monitoring, $r^2$ of nitrogen, moisture, and carbohydrate in 'CY2' creeping bentgrass was 0.840, 0.904, and 0.944, respectively. SEP was 0.066, 1.868, and 0.601, respectively. After outlier treatment, $r^2$ was 0.892, 0.925, and 0.971, while SEP was 0.052, 1.577, and 0.394, respectively, which totally showed a high correlation. However, $r^2$ of starch was 0.464, which appeared a low correlation. Thereof, the verified equation appearing higher $r^2$ of nitrogen, moisture, and carbohydrate showed its higher accuracy of prediction model, which finally could be put into practical use for turf management system.

The Analysis of Statistics and Scientific Inquiries Types in Korea Astronomy and Space Science Institute Q&A Service (한국천문연구원 질문상자의 통계 및 과학탐구 질문유형 분석)

  • Yim In Sung;Sung Hyun Il;Sohn Sangmo;Ahn Young Sook;Kim Bong Gyu;Choe Seung-Urn
    • Journal of the Korean earth science society
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    • v.26 no.6
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    • pp.551-559
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    • 2005
  • The Q&A service of the official Korea Astronomy and Space Science Institute (KASI) webpage was installed in 2000 and have been actively used since then. In this paper, we analyze the questions asked through the Q&A service and the number of inquiries with the aid of statistical methods. We also study the contents of the questions. Specifically, we have created statistics of questions and inquiries that go monthly and yearly, and have developed categories to analyze the characteristics of questions in regards to their cognitive aspects. Each question is categorized into two elements based on their recognitive aspect: science knowledge or science study. Each element also has sub-categories that help readers understand the characteristics of the questions. For the analysis, we used a sample consisted of questions from July to December, 2004. Through this study, we achieved a better understanding of the questions asked by the Q&A service. We are planning to improve the quality of the Q&A service by extending the size of the FAQ(frequently asked questions). Throughout this study, we find that the number of questions are increasing with time, and the overall quality of the questions is improving. As we expect the number of people using our Q&A service to increase and the questions to get more difficult to answer, development of improved content is required.

Characteristics of Ti-SPAC as Fluidizing Phase Photocatalyst (Ti-구형활성탄의 유동상 광촉매 특성 평가)

  • Lee, Joon-Jae;Suh, Jeong-Kwon;Hong, Ji-Sook;Park, Jin-Won;Lee, Jung-Min
    • Korean Chemical Engineering Research
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    • v.44 no.4
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    • pp.375-381
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    • 2006
  • In this sturdy, spherical activated carbon(SPAC) contained $TiO_2$ was made by ion-exchanged treatment and heat treatment for applying fluidizing bed system. The ion-exchange resin was treated by $TiCl_3$ aqueous solution. The treated resin and raw resin were heat-treated under nitrogen condition to convert into Ti-SPAC. During the heat-treatment, burn-off weight amounts and the element were measured by means of TGA and TGA/MS, individually. The physicochemical properties of Ti-SPAC was characterized by means of XRD, SEM, EDS, BET, EPMA, ESR, intensity and titanium content. The Ti-SPAC had spherical shape with diameter size about $350{\mu}m{\sim}400{\mu}m$ and $617m^2/g$ specific surface area. Structure of $TiO_2$ in Ti-SPAC was anatase and rutile form. Also, $TiO_2$ on SPAC were found that the $TiO_2$ were uniformly distributed through EPMA analysis. Moreover, the Ti-SPAC showed indirect photocatalyst activity estimation through ESR analysis, characteristics of photocatalyst potentially. Over all results, Ti-SPAC was used in fluidizing bed UV/photocatalyst system to remove HA(Humic Acid). That results were HA removal efficiency was about 70% and Ti-SPAC intensity was preserved during reaction. Ti-SPAC showed practical possibility as photocatalyst in fluidizing bed system.

Comparison of Reservoir Sediment in Kum River Basin

  • Hwang, Jong Yeon;Han, Eui Jung;Kim, Tae Seung;Kim, Tae Keun;Yu, Soon Ju;Kim, Shin Jo;Yoon, Young Sam;Chung, Yong Soon
    • Journal of Environmental Impact Assessment
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    • v.8 no.1
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    • pp.107-120
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    • 1999
  • This study was performed to survey the general feature of reservoir sediment in Daecheong, Sapkyo and Kumkang reservoir. For investigations, sediments were sampled at four sites in Dae-Cheong and two sites in SapKyo and Kumkang in June and October. The items for investigation are as follows; water content, loss on ignition(IG), porosity of sediment, contents of element such as hydrogen, nitrogen, carbon, phosphorus contents and nutrient release rates. Loss on ignition was measured to determine the contents of organic substance. And its ranges were determined 2.4~16.2% in Daecheong reservoir, 5.6~27.9% in Sapkyo and 4.8~18.7% in Kumkang reservoir. And, total phosphorus contents in sediments were measured $677{\sim}5,238{\mu}g/g$ in Daecheong, $780{\sim}1,417{\mu}g/g$ in Sapkyo and $604{\sim}1,452{\mu}g/g$ in Kumkang reservoir. And release rates of nutrients were calculated $0.05{\sim}8.63mgP/m^2day$ and $4.99{\sim}36.56mgN/m^2day$ in Daecheong, $1.83{\times}10^{-3}{\sim}3.23{\times}10^{-2}mgP/m^2day$ and $1.97{\sim}.22mgN/m^2day$ in Sapkyo, $8.31{\times}10^{-3}{\sim}6.51{\times}10^{-2}mgP/m^2day$ and $0.89{\sim}4.42mgN/m^2day$ in Kumkang reservoir, respectively. And this study attempted to determine the humus level of sediments. As a result of elemental analysis, C/N ratio was determined 3.0~13.1 in Daecheong reservoir, 6.5~13.0 in Sapkyo and 3.6~12.6 in Kumkang reservoir, respectively. From the elemental analysis, humus levels of reservoirs were changed from mesohumic to oligohumic state in all reservoirs.

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A Case Study of Display Design of Space 'O'sulloc Teahouse' from the Point of Sequence Narrative (시퀀스 내러티브 관점에 따른 '오설록 티하우스' 공간의 제품 전시 디자인 사례 연구)

  • Yang, Hyeon-Jeong;Lee, Hyunsoo
    • Korean Institute of Interior Design Journal
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    • v.23 no.1
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    • pp.61-68
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    • 2014
  • Recently, there have been increasing attempts to pursue and express feelings such as sensibilities, emotions, and impressiveness in commercial spaces. One of such methods is to apply 'storytelling' to space designs. Applicability of storytelling to a space suggests that the contents of a space can be expressed through various mediums. Portraying events and situations through a single time continuity of a story is referred to as 'narrative'. The movement of users and sequence of contents are determined by a narrative. It provides different storytelling and a sense of place to each space through various roles, such as wide association, engraving, and image formation. A narrative can lead users to engage in different perceptions and behaviors even in spaces with the same content. Thus, this study is intended to examine the impact of space marketing in line with design narratives, assuming that narratives of commercial space designs will influence the formation of brand identity. The research methods are as follows. First, the definition of narratives in space design was established by examining narrative architectures. Second, design analysis tools for commercial spaces were established from the perspective of narratives through preceding studies. Third, the design narratives of different shops under the same brand were comparatively analyzed through a case study. To carry out a case study, a commercial space of 'O'sulloc' was selected, and its brand identity was studied from the narrative standpoint. The case study involved interior designs of 7 road shops of 'O'sulloc.' Among the 7 road shops, two of them with the biggest difference in design narratives were selected, and an observation survey was done on the users as a second analysis. Through the observation survey, actual design narrative experience was analyzed in 4 steps of introduction, development, turn, and conclusion. The findings are as follows. The design method of each shop varied, and different design elements were emphasized. Among various elements, the ones that reflect the brand identity of 'O'sulloc' the best were logo, product, and shape. During the process of narratives, the characteristics of each shop and user recognition and behavior varied depending on the degree of emphasis on a particular element. It suggests that space design narratives can influence the formation of brand identity. This study provides ideal directions of developing space designs necessary for forming brand identity from the standpoint of Korean traditional culture modernization. Future studies could discuss the economic feasibility of such designs.