• Title/Summary/Keyword: Contamination process

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Effects of a Soaking-Fermentation-Drying Process on the Isoflavone and ${\gamma}$-Aminobutyric acid Contents of Soybean

  • Kim, Tae-Jin;Sung, Chang-Hyun;Kim, Young-Jin;Jung, Byung-Moon;Kim, Eung-Ryool;Choi, Won-Sun;Jung, Hoo-Kil;Chun, Ho-Nam;Kim, Woo-Jung;Yoo, Sang-Ho
    • Food Science and Biotechnology
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    • v.16 no.1
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    • pp.83-89
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    • 2007
  • In our study, lactic acid bacteria (LAB)-fermented whey solutions were applied in the soybean soaking process to minimize bacterial contamination and to enrich the biologically functional components of isoflavone and $\gamma$-aminobutyric acid (GABA). Among the 11 LAB tested, Bifidobacteria infantis and a mixed culture (Lactobacillus acidophilus, Bifidobacteria lactis, and Streptococcus thermophilus; ABT-3) displaying the greatest $\beta$-glucosidase activity were selected to produce improved biologically functional soybean preparations. In the soybean soaking processing (without water spraying), the LAB-cultured 10% whey solution was used to soak and to ferment the soybeans and the fermented soybeans were finally dried by heat-blowing at $55^{\circ}C$. The processing conditions used in this study demonstrated that the final soybean product had a reduced contamination by aerobic and coliform bacteria, compared to raw soybeans, likely due to the decrease in pH during LAB fermentation. The aglycone content of the isoflavone increased up to 44.6 mg per 100 g of dried soybean by the processing method, or approximately 8-9 times as much as their initial content. The GABA contents in the processed samples increased as the processing time of soaking-fermentation proceeded as well. The soybean sample that fermented by ABT-3 culture for 24 hr showed the greatest increase in GABA content (23.95 to 97.79 mg/100 g), probably as a result of the activity of glutamate decarboxylases (GAD) released from the soybean or produced by LAB during the soaking process.

Characterization of Salmonella species from poultry slaughterhouses in South Korea: carry-over transmission of Salmonella Thompson ST292 in slaughtering process

  • Yewon Cheong;Jun Bong Lee;Se Kye Kim;Jang Won Yoon
    • Journal of Veterinary Science
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    • v.25 no.3
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    • pp.39.1-39.11
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    • 2024
  • Importance: Salmonella outbreaks linked to poultry meat have been reported continuously worldwide. Therefore, Salmonella contamination of poultry meats in slaughterhouses is one of the critical control points for reducing disease outbreaks in humans. Objective: This study examined the carry-over contamination of Salmonella species through the entire slaughtering process in South Korea. Methods: From 2018 to 2019, 1,097 samples were collected from the nine slaughterhouses distributed nationwide. One hundred and seventeen isolates of Salmonella species were identified using the invA gene-specific polymerase chain reaction, as described previously. The serotype, phylogeny, and antimicrobial resistance of isolates were examined. Results: Among the 117 isolates, 93 were serotyped into Salmonella Mbandaka (n = 36 isolates, 30.8%), Salmonella Thompson (n = 33, 28.2%), and Salmonella Infantis (n = 24, 20.5%). Interestingly, allelic profiling showed that all S. Mbandaka isolates belonged to the lineage of the sequence type (ST) 413, whereas all S. Thompson isolates were ST292. Moreover, almost all S. Thompson isolates (97.0%, 32/33 isolates) belonging to ST292 were multidrug-resistant and possessed the major virulence genes whose products are required for full virulence. Both serotypes were distributed widely throughout the slaughtering process. Pulsed-field gel electrophoretic analysis demonstrated that seven S. Infantis showed 100% identities in their phylogenetic relatedness, indicating that they were sequentially transmitted along the slaughtering processes. Conclusions and Relevance: This study provides more evidence of the carry-over transmission of Salmonella species during the slaughtering processes. ST292 S. Thompson is a potential pathogenic clone of Salmonella species possibly associated with foodborne outbreaks in South Korea.

Investigation of Microbial Contamination in Liriope platyphylla at Post Harvest Environments (맥문동(Liriope platyphylla) 수확 후 처리 환경의 위생지표세균 및 병원성 미생물 오염도 조사)

  • Kim, Yeon Rok;Ha, Ji-Hyoung;Kim, Se-Ri;Park, Young Chun;Kim, Kyeong Cheol;Kim, Won-Il;Ryu, Song Hee;Kim, Hwang-Yong
    • Journal of Food Hygiene and Safety
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    • v.31 no.2
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    • pp.99-106
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    • 2016
  • This study was aimed to assess microbiological contamination level of Liriope platyphylla farms (A, B, and C) located in Cheongyang, Chungnam province. Specimens sampled from those farms and L. platyphylla tuberous roots were assessed for sanitary indication bacteria such as total aerobic bacteria, coliforms, and Escherichia coli and pathogenic bacteria such as Escherichia coli O157:H7, Listeria monocytogenes, Salmonella spp., Staphylococcus aureus and Bacillus cereus, quantitatively and qualitatively. As a result, those farms are not contaminated by E. coli O157:H7, L. monocytogenes, Salmonella spp., at all. And S. aureus was only found qualitatively from workers' gloves at a farm. As a whole, those farms (soil, harvest container, harvester, cleanser, washing water and tray) were maintained in a low level of microbiological contamination. However a cleanser was contaminated by coliforms ($4.35log\;CFU/100cm^2$), and it is required to improve farm hygiene. Microbiological contamination level of L. platyphylla tuberous root was decreased in the postharvest process including washing and drying.

Site Application Characteristics of Deep-Site Biopile System for Cleaning Oil-Contaminated Soil/Underground Water (유류오염 토양/지하수 정화를 위해 개발된 DSB(Deep-Site Biopile) System 현장적용특성)

  • Han Seung-Ho;Kong Sung-Ho;Kang Jung-Woo
    • Journal of Soil and Groundwater Environment
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    • v.10 no.2
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    • pp.28-34
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    • 2005
  • The aim of this article is to assess the application characteristics of the site by remediating oil-contaminated area using DSB (Deep-site Biopile) system. In the contaminated area, the soil was composed of penetrable sand and the leaked oil was spread widely (total 7,201 cubic meters) through 2.5 meter deep underground water flow. DSB system was operated for 30 minutes intervals for 24 hours in a day (30 minutes opεration and 30 minutes stop). To check contamination level change in the contaminated area after DSB system was operated, samples were taken. The result from the site shows that BTEX/TPH contamination level was dropped 50% after 30-day operation of DSB system, and that contamination level was dropped below contamination level check standard after 165 days and the remediation was completed. Unlike traditional biological remediation methods DSB system could efficiently process soil and water which were contaminated by high levels of oil compounds.

A Study of Carry Over Contamination in Chematology (이월오염에 대한 연구)

  • Chang, Sang-Wu;Kim, Nam-Yong;Lyu, Jae-Gi;Jung, Dong-Jin;Kim, Gi-You;Park, Yong-Won;Chu, Kyung-Bok
    • Korean Journal of Clinical Laboratory Science
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    • v.37 no.3
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    • pp.178-184
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    • 2005
  • Carry over contamination has been reduced in some systems by flushing the internal and external surfaces of the sample probe with copious amount of diluent. It between specimens should be kept as small as possible. A built-in, continuous-flow wash reservoir, which allows the simultaneous washing of the interior and exterior of the syringe needles, addresses this issue. In addition, residual contamination can further be prevented through the use of efficient needle rinsing procedures. In discrete systems with disposable reaction vessels and measuring cuvets, any carry over is entirely caused by the pipetting system. In analyzers with reuseable cuvets or flow cells, carry over may arise at every point through which high samples pass sequentially. Therefore, disposable sample probe tips can eliminate both the contamination of one sample by another inside the probe and the carry over of in specimen into the specimen in the cup. The results of the applicative carry over experiment studied on 21 items for total protein (TP), albumin (ALB), total bilirubin (TB), alkaline phosphatase (ALP), aspratate aminotranferase (AST), alanine aminotranferase (ALT), gamma glutamyl transferase (GGT), creatinine kinase (CK), lactic dehydrogenase (LD), creatnine (CRE), blood urea nitrogen (BUN), uric acid (UA), total cholesterol (TC), triglyceride (TG), glucose (GLU), amylase (AMY), calcium (CA), inorganic phosphorus (IP), sodium (Na), potassium (K), chloride (CL) tests in chematology were as follows. Evaluation of process performance less than 1% in all tests was very good, but a percentage of ALB, TP, TB, ALP, CRE, UA, TC, GLU, AMY, IP, K, Na, and CL was 0%, implying no carry over. Other tests were ALT(-0.08%), GGT(-0.09%), CK(0.08%), LD(0.06%), BUN(0.12%), TG (-0.06%), and CA(0.89%).

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Examination of Soil Contamination Status and Improvement Strategies within Urban Development Projects (도시개발사업 내 토양 오염 현황과 개선 방안 고찰)

  • Heo, Sujung;Lee, Dong-Kun;Kim, Eunsub;Jeon, Seong-Woo;Jin, Zhiying
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.27 no.3
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    • pp.45-56
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    • 2024
  • Heavy metals emitted from urban development do not decompose in the soil and remain for long periods, continually impacting the environment. Since the mid-1990s, there has been increasing societal concern in South Korea regarding soil contamination, prompting various legislative revisions to reduce pollution. This study utilizes the Environmental Impact Assessment Support System (EIASS) to investigate projects in the metropolitan area that have exceeded the Ministry of Environment's soil contamination concern levels from 1989 to 2022 and to examine improvements in the environmental impact assessment (EIA) process. The results reveal that the average concentrations of nine contaminants-cadmium (Cd), copper (Cu), arsenic (As), mercury (Hg), lead (Pb), hexavalent chromium (Cr6+), zinc (Zn), nickel (Ni), and fluoride (F)-have all increased over the years. Among these, Zn had the highest relative proportion, with 37.5% of the 40 sites exceeding environmental concern levels. Investigation of 19 specific projects at these exceedance sites showed that only 7 had documented analyses of contamination causes and remediation plans, and just one had contracted additional remediation services, though results from these efforts were found to be lacking. Furthermore, since 2019, a significant proportion of these sites were involved in residential developments, likely due to government initiatives in new city development and extensive housing supply plans. This research emphasizes the importance of public disclosure of the processes and outcomes of remediation efforts on historically contaminated soils prior to project development. It discusses improvements to the EIA by reviewing current legislation and international examples. The findings of this study are expected to heighten public awareness about heavy metal contamination and enhance transparency in soil remediation efforts, contributing to sustainable environmental management and development.

Recycling of Wastepaper(XV) -Contamination of Process Water by System Closure- (고지재생연구(제15보) -제지 공정수 폐쇄화에 따른 오염 및 변화에 관한 연구-)

  • 조미선;윤혜정;류정용;신종호;송봉근
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.33 no.2
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    • pp.32-40
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    • 2001
  • It is desirable to reduce the amounts of fresh water and reuse the recycled water in papermaking process. In an attempt to improve the efficiency of recycling water refining treatment and to enhance the productivity of OCC recycling mill, up flow anaerobic sludge blanket(UASB) reactor was developed and introduced to Korea recently. In order to maximize the operating efficiency and minimize the adverse side effects it is imperative to estimate and evaluate the total effects of new system, UASB on the whole OCC recycling process. This study was carried out with a view to investigate the effects of the high temperature and alkalinity of process water on the quality ad productivity of testliner of OCC recycling mill which is equipped with UASB reactor and almost closed. Another object of this study was searching for the best available use of UASB treated process water. The results were as follows; Reuse of UASB treated water characterized with high temp and alkalinity was useful to improve the strength and drainage properties of recycled OCC owing to its good points of promoting OCC disintegration and reducing the calcium hardness of process water. However, it might not be avoidable to induce the increase of dissolved solids in process water in accordance with direct introducing of UASB treated water into the former stage of OCC stock preparation. So it would be advisable to adopt the UASB treated water in the stage of clean, screened OCC stock.

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Nitrate Removal and Recycling Technique (질산 제거 및 재이용 기술)

  • Sim, Sang Jun;Lee, Kyung Hee;Cho, Young Sang
    • Clean Technology
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    • v.3 no.2
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    • pp.31-33
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    • 1997
  • Nitrate contamination in surface water and ground water have increased in Korea. This trend has raised concern because nitrates caused methemoglobinemia in infants. To remove nitrates from waters, various purification processes including ion-exchange, biological denitrification, and chemical denitrification are currently in use for the treatment of water. However, little economically advantageous process exists for the industrial scale treatment of effluents highly polluted with nitrates. A new process has been developed for nitrate and other salts removal from polluted waters. Alumina cement and lime served as precipitating agents to remove nitrate with stirring at basic pH. Decreasing alumina content in alumina cement result in a increasing in nitrate removal yield. Stable removal of nitrate(1000mg/L) was readily achieved by two-stage removal process.

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A Study on Solar Cell Wafer Cleaning using Ozonate Water (오존수를 이용한 태양전지용 웨이퍼의 세정에 관한 연구)

  • Moon, Se-Ho;Chai, Sang-Hoon;Son, Young Su
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.11
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    • pp.43-49
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    • 2013
  • We have studied on ozonate water cleaning mechanisms to apply in manufacturing process of 156 mm silicon wafer which is used in the solar cell fabrication. We have analyzed contamination sources on wafer surface which causes poor quality and performance of products in fabrication process, and examined cleaning process using ozonate water to eliminate it. Using this novel technology particles are removed over 94%, and remained organic materials are removed more over 45%.

Analysis of dismantling process and disposal cost of waste RVCH

  • Younkyu Kim;Sunkyu Park ;TaeWon Seo
    • Nuclear Engineering and Technology
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    • v.55 no.1
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    • pp.45-51
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    • 2023
  • During the operation of a nuclear power plant (NPP), the waste reactor vessel closure head (RVCH) that is replaced owing to design or manufacturing defects is buried in a designated area or temporarily stored in a radiation shielding facility within the NPP. In such cases, storing it for extended periods proves a challenge owing to space constraints in the power plant and a safety risk associated with radiation exposure; therefore, dismantling it quickly and safely is crucial. However, not much research has been done on the dismantling of the RVCH in an operational power plant. This study proposes a dismantling process based on the radioactive contamination level measured for the Kori #1 RVCH, which is currently being discarded and stored, and examines the decontamination and cutting according to this process. In addition, the amount of secondary waste and dismantling cost are evaluated, and the dismantling effect of the reactor closure head is analyzed.