• Korean Journal of Health Education and Promotion
• /
• v.16 no.1
• /
• pp.137-148
• /
• 1999

The necessity of school health promoting projects is well known to many researchers, however, there is a lack of research on the development of strategies for school health programs and the evaluation of the effectiveness of the programs. The results of our previous study in 1996 identified poor eyesight as one of the several health problems among elementary school children in urban areas. The main objective of this study is to develop, implement, and evaluate vision care for elementary school children. The research method utilized a quasi experimental design using non-equivalent group, pre-post comparison. The sample consisted of all second grade children in two elementary schools in Kyung-giProvince. In order to prevent a contamination effect of the vision care program, one school was selected as a control and the other school was selected as a treatment group. The experimental school had 467 children and the control school had 402 children. The visual acuity of children in both group was measured and a survey questionnaire was sent out to parents on the knowledge, attitude, and behavior related to vision care before the program was implemented. The children of the experimental school received the following programs; separate education on proper nutrition and care of eyesight for children and parents, daily eye exercises using video tape, and an essay and poster contest on healthy eyesight. The eye exercise program lasted from March, 1997 to November, 1997. On November, 1997, visual acuity was measured again from children in both schools. The data was analyzed by using SAS statistical package. The results indicate that the children in the experimental group had significantly (p〈0.001) better eyesight than the children in the control group. Also, some of the behavior highly related to the poor eyesight are poor maternal eyesight and watching TV from a short distance. In conclusion, one of the significance of this project is that the vision care program was developed after the need assessment. It will be encouraged in the future that more school health program should be developed after a need assessment. Also, inclusion of family members was important in health promoting projects within a school setting since healthy behavior needs to be reinforced at horne as well as at school.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.38 no.2
• /
• pp.118-128
• /
• 2005

Surface sediment samples from 31 stations in Jinhae Bay were analyzed to evaluate the pollution by sewage from the spatial distribution of the fecal sterol, coprostanol. The sums of eight sterols (coprostanol, epicholestanol, epicoprostanol, cholesterol, cholestanol, brassicasterol, stigmasterol and $\beta$-sitosterol) were in the range of $2,703-27,154{\cal}ng/{\cal}g$ dry weight. The concentrations of the coprostanol in the sediments, which is a good tracer of sewage-derived organic contamination, were in the range of $76-3,964{\cal}ng/{\cal}g$ dry weight. The levels of coprostanol were much higher (almost one order of magnitude) at stations close to the big cities such as Masan and Jinhae. However, those levels were comparable to or lower than those previously reported in other foreign locations. Some ratios of coprostanol to other sterols and multivariate analysis could provide us with the information on the origin of the sterols being from sewage, plankton, and/or benthos. Those showed sewage dominance at the stations near the cities Masan and Jinhae, plankton dominance at the center of Jinhae Bay and mainly marine fauna origin at the mouth of Jinhae Bay. These results suggest that the contents of sterols and some ratios of these components are quite powerful tracer for the detection of the origin of the organic contaminants in the coastal environments.

• Journal of Korean Society of Environmental Engineers
• /
• v.33 no.7
• /
• pp.501-510
• /
• 2011

In this study, water pollution sources of 14 major tributaries of Han river and characteristics of water quality for each target streams were evaluated based on water quality data in 2007.1-2009.12 (14 data sets) using a statistical package, SPSS-17.0. Cluster analysis over time and space for each stream resulted in 4 groups for the spatial variations in which type and density of pollution sources in the basins showed the greatest impact on grouping. Moreover, cluster analysis for the time variation in which rainfall, temperature and eutrophication were shown to contribute to the clustering, produced 2 groups, from summer to fall (July-Oct.) and from winter to early summer (Nov.-June). Four factors were found as responsible for the data structure explaining 71-90% of the total variance of the data set depending on the streams and they were organic matter, nutrients, bacterial contamination. Factor analysis showed main factors (water pollutants) changed according to the season with different pattern for each stream. This study demonstrated that water quality of each stream could produce useful outcomes when factor and pollution source of basin were evaluated together.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.42 no.5
• /
• pp.442-448
• /
• 2009

Bacteriological examination of a 12,700 ha area within Jaranman Saryangdo area, located in Jaran bay and Saryangdo island, was conducted with 35 fixed (designated areas and adjacent areas) seawater sampling stations between January 2006 and December 2008. According to results, the geometric mean range and the estimated 90th percentile range of fecal coliform counts in sea water samples collected in the designated area were <1.8-2.4 and <1.8-8.6 MPN/100mL, respectively. The estimated 90th percentile range of fecal coliform counts in sea water samples collected from 6 sampling stations in the adjacent areas were 6.1-34.6 MPN/100mL. Based on these results, bacteriological water quality of the designated areas in the Jaranman. Saryangdo area meet the NSSP (National Shellfish Sanitation Program) guidelines for approved area. The bacteriological sea water quality in Jaranman.Saryangdo area has been shown to be favorable at all investigated sampling stations except for July and August which coincided with heavy rainfall. In fact, the bacteriological water quality was not affected if rainfall was less than 30 mm rainfall. However, the degree of bacteriological contamination increased rapidly and the water quality exceeded approved NSSP guidelines set for rainfall above 60 mm.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.48 no.5
• /
• pp.621-630
• /
• 2015

This study investigated the concentration of total mercury in 17 species of commonly consumed fishes and assessed the risk to human health from provisional tolerable weekly intakes PTWI% as affected by migratory characterization, such as migration and vagility. The measured mean concentrations of total mercury in these 17 species of commonly consumed fishes suggest that mean concentrations of total mercury in 10 species of migratory fishes (largehead hairtail, chub mackerel, Pacific saury, skipjack tuna, Pacific cod, anchovy, Alaska pollack, brown croaker, Japanese Spanish mackerel, yellow croaker and Pacific herring) were low compared to those in 7 species of demersal fishes (red stingray, brown sole, bastard halibut, conger eel, blackmouth angler, rockfish and filefish). Based on the mean concentrations, the PTWI% of total mercury among commonly consumed migratory fishes was 3.393%, which was higher than that of commonly consumed demersal fishes (2.710%).

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.45 no.2
• /
• pp.104-113
• /
• 2012

Crab-like flavorants (CFs) were made from snow crab cooker effluent (SCCE) using response surface methodology (RSM) and reaction flavoring technology (RFT). Type A CF was made from SCCE via RSM, RFT, adding starch syrup, centrifugation, and microfiltration. Type B was made from type A by adding the food additives dimethyl sulfide, ethyl valerate and fish sauce. The stability of the CFs was evaluated in terms of the color values, sensory evaluation, and flavor profiles after storage for 90 days at three different temperatures: 10, 20, and $30^{\circ}C$. The compounds, ethanol and 3-methyl-1-butanol, were considered key components of off-flavor and a decrease in dimethyl-2-vinylpyrazine affected the occurrence of off-flavor. It may be a microbial metabolite arising from contamination and lab-scale micro-filtration. At the lowest temperature ($10^{\circ}C$), the decrease in volatile compounds, such as pyrazines, was not as dramatic as at $20^{\circ}C$ and $30^{\circ}C$ and alcohol formation was prevented or delayed. Therefore, it is necessary to store CFs at < $10^{\circ}C$ with suitable sterilization to preserve volatile flavor compounds and prevent off-flavor from occurring.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.47 no.5
• /
• pp.508-515
• /
• 2014

The contamination status of Vibrio parahaemolyticus in commercially valuable shellfish from the south and west coasts of Korea and the antimicrobial resistance patterns of isolated V. parahaemolyticus were investigated from July through October, 2011. The range of V. parahaemolyticus concentrations in oysters Crassostrea gigas and short neck clams Venerupis philippinarum was <30~290 MPN/100 g and <30~46,000 MPN/100 g, respectively, and greater than 10,000 MPN/100 g of V. parahaemolyticus was detected from 7 of 40 short neck clams. During the survey period, 436 strains of V. parahaemolyticus were isolated (129 from oysters and 307 from short-neck clams) and the antimicrobial resistance patterns of all of the isolates were examined. Antimicrobial resistance against at least one antibiotic was seen in 79.8% of the oyster isolates (103 strains) and 63.8% of the short neck clam isolates (196 strains). The antimicrobial resistance patterns were relatively simple because the antimicrobial resistance of the isolates was simply due to resistance to ampicillin. Only one oyster isolate and three short neck clam isolates showed multiple antibiotic resistance, i.e., resistance against more than four antibiotics.

• Environmental Engineering Research
• /
• v.14 no.4
• /
• pp.250-254
• /
• 2009

Contamination of microcystins, a family of heptapeptide hepatotoxins, in eutrophic water bodies is a worldwide problem. Due to their poisoning effects on animals and humans, there is a requirement to characterize and quantify all microcystins present in a sample. As microcystins are, for most part, intracellular toxins produced by some genera of cyanobacteria, lysing cyanobacterial cells to release all microcystins is considered an important step. To date, although many cell lysis methods have been used, little work has been conducted comparing the results of those different methods. In this study, various methods for cell lysis and toxin extraction from the cell lysates were investigated, including sonication, bead beating, freeze/thaw, lyophilization and lysing with TritonX-100 surfactant. It was found that lyophilization, followed by extraction with 75% methanol, was the most effective for extracting toxins from Microcystis aeruginosa cells. Another important step prior to the analysis is removing impurities and concentrating the target analyte. For these purposes, a C18 Sep-Pak solid phase extraction cartridge was used, with the percentage of the eluent methanol also evaluated. As a result, methanol percentages higher than 75% appeared to be the best eluting solvent in terms of microcystin-leucine-arginine (MC-LR) recovery efficiency for the further chromatographic and mass spectrometric analyses.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.25-25
• /
• 2018

The Fukushima Daiichi Nuclear Power Plant accident in March 2011 caused severe radioactive contamination in the surrounding environment. Since the accident, much attention has been paid to the biological and genetic consequences of organism inhabiting the contaminated area. The effect of radiation exposure on genetic mutation rates is little known, especially for low doses and in situ conditions. Evaluating DNA mutation by low levels of radiation dose is difficult due to the rare mutation event and lack of sequence information before the accident. In this study, correlations with air dose levels and somatic DNA mutation rates were evaluated using Next Generation Sequencer for the clonal plant, Phyllostachys edulis. This bamboo is known to spread an identical clone throughout Japan, and it has the advantage that we can compare genetic mutation rate among identical clone growing different air dose levels. We collected 94 samples of P. edulis from 14 sites with air dose rates from $0.04{\sim}7.80{\mu}Gy/h$. Their clonal identity was confirmed by analysis using 24 microsatellite markers, and then, sequences among samples were compared by MIG sequence. The sequence data were obtained from 2,718 loci. About ~200,000 bp sequence (80 bp X 2,718 loci) were obtained for each sample, and this corresponds to about 0.01% of the genome sequence of P. edulis. In these sequences, 442 loci showed polymorphism patterns including recent origin mutation, old mutation, and sequence errors. The number of mutations per sample ranged from 0 to 13, and did not correlate with air dose levels. This result indicated that DNA mutations have not accumulated in P. edulis living in the air doses levels less than $10{\mu}Gy/h$. Our study also suggests that mutation rates can be assessed by selecting an appropriate experimental approach and analyzing with next generation sequencer.

• Applied Biological Chemistry
• /
• v.22 no.1
• /
• pp.18-23
• /
• 1979

To prepare the objective quality control index of red pepper powder, the relationships among the color appearance, capsanthin red pigment and the contamination of coliform bacteria were studied and summarized as followings; 1. Visual method by human eyes was inadequate to grade the quality of red pepper powder, because of the different personal color evaluation. 2. Grading upon capsanthin contents are well agreed with the color appearance of the red pepper powder. Therefore, color appearance can be correlated with the capsanthin content. 3. Color appearance of the red pepper can be numerically expressed with Hunter-value a/b; Capsanthin $content(mg/g-red pepper)=0.257{\times}10^{0.703}(a/b)$ and it can be used as an index of the quality control of red pepper powder. 4. There was no distinct correlation between the comtamination of coliform bacteria and the color value.