• YAKHAK HOEJI
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• v.31 no.2
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• pp.116-125
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• 1987

It is still difficult and time consuming to obtain cDNA sequences that contain the entire nucleotide sequence of the corresponding mRNA. A rapid and high efficient cloning method to obtain full-length cDNA segments is thus developed. The cloning procedure described here consists of the construction of oligo(dT)-tailed vector primer using pWR34 plasmid, polyadenylation of mRNA-cDNA heteroduplex using terminal deoxytransferase, and replacement of MRNA strand with DNA by RNase H and DNA polymerase I. The restriction endonuclease analysis shows that the size of inserted-cDNA is in the range of 1.5~4.0 kb long suggesting that most of cloned cDNA are full-length or nearly full-length cDNA. The plasmid-DNA recombinants obtained were 4$\times$$10^5$~$10^{6}$ per $\mu\textrm{g}$ of rat liver poly (A$^+$)mRNA, which is 4 to 10 fold higher cloning efficiency in comparison to the presently used methods for full-length cDNA cloning. The results indicate that the described cloning system is much simpler, less time consuming, and very efficient cloning method to construct a cDNA library.

• Journal of Broadcast Engineering
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• v.18 no.1
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• pp.98-105
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• 2013

Patterns for generating and consuming contents are various in these days from conventional broadcasting contents to UCC. There are many researches on developing recommendation engines based on user's profile for providing desired contents. In this paper we propose a contents recommendation system using not only user's profile but other's profiles in closed user group of the social network based on patterns for user's consuming contents. The proposed recommendation agent update user's profile using usage history and other's profiles related to the user in the closed user group.

• Journal of the Korean Society of Safety
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• v.31 no.1
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• pp.41-47
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• 2016

The rolling stock is composed of several cars. In order to operate in combination, it is necessary to connect the device, called coupler, between the rolling stocks. When the collision occurs between cars, couplers should be able to absorb the shock. Urban railway has used only rubber absorbers. But recently, the hydraulic buffer has been considered in general railway. In order to know the performance of the buffer it should be conducted to experiments. But whenever this combination change, we should experiments to know a lot of the dynamic behavior of each coupler. These experiments are generally replaced by the simulation, since a lot of time and cost consuming. The quasi-static map of hydraulic buffer obtained by the experiments is required for the simulation. However, the experiments for obtaining such a quasi-static map is costly and time consuming. In this paper, it proposes a method for deriving the quasi-static map of hydraulic buffer from the theoretical model.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.5
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• pp.1164-1170
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• 2010

IPTV hasn't fulfilled the expectations that it will substitute existing digital broadcasting service and serve new type of services such as interactive and multi-channels. Today's IPTV service just seems to change existing service platform to new one. In this situation, Open IPTV service has been on the rise, which make it possible for consumers to choice contents regardless of contents providers. However, the integration of variety service platforms such as rights protection solution is essential to provide Open IPTV service. In this paper, authoring and consuming system for package contents based on MPEG-21 Multimedia Framework which is developed for distributing media resources.

• Journal of The Korean Association of Information Education
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• v.23 no.5
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• pp.461-467
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• 2019

As the convergence solutions become more common, the use of Arduino and Raspberry Pi boards has been increasing. These control boards has to be executed under power blackout. In this environment, we take advantage of solar power system to overcome the power out. In this paper, we poposed a effficient power control strategy. Our experimental device is a DSLR shooting device executed based a predesigned interval time. The control module of our experimental device is the compound system of Raspberry Pi and Arduino boards. Arduino board send the force signals to wake up Raspberry Pi. We developed a new control strategy algorithm for the efficient use of solar power energy. In this paper, we mesured the efficiency of solar enery consuming of our system. We programmed a control system to send DSLR shooting signals. In experimentals, we ensured a stable consuming of electricity during 10 days. In the end, it was found that the consumption power of the Raspberry was reduced by about 81% when the Aduino was combined to save power.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.487-488
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• 2007

Fault section detection and service restoration is very important in ungrounded distribution system. Techniques currently used to track down faults are time consuming and cumbersome. A new scheme is developed based on communication technology, and the simulation result shows that the method can satisfy the requirement proposed.

• Journal of Korea Multimedia Society
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• v.13 no.10
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• pp.1413-1422
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• 2010

With the emergence of digital TV broadcasting, various channels are provided to a TV audience. But it is getting hard for the audience to find his or her preferred TV programs due to the huge number of TV channels. In order to mitigate the difficulty, TV broadcasting companies provide an electronic program guide (EPG), which is a digital guide to scheduled broadcast TV programs. However, it results in the information overload problem and the time-consuming problem since the number of TV channels and programs is gradually on the increase. In this paper, we design and develop a channel filtering system, which recommends a small number of channels by filtering TV channels based on the watching pattern of the TV audience. The channel filtering system does not require the replacement or upgrade of existing TV or set-top box. In addition, it increases usability by skipping the channels that broadcast the audience's non-preferred TV programs while the TV audience presses the channel up/down button.

• 제어로봇시스템학회:학술대회논문집
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• 2002.10a
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• pp.120.6-120
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• 2002

This paper presents an application of rule based expert system to GDS. Expert system is a computer-based system that uses knowledge, facts, and reasoning techniques to solve problems that normally require the abilities of human experts. Expert system can be classified as synthesis expert system, classification expert system or combination of these. The Expert system of GDS belongs to the synthesis type of expert system. GDS is a graphic design environment that automates complex and time consuming processes in the design phase of the grating production. There are methodical procedures in the design phase of gratings and each process needs the know-how of a skillful designer. User has to know...

• Journal of the Korean Society for Precision Engineering
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• v.16 no.7
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• pp.210-218
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• 1999

Sheet deposition type rapid prototyping system have many advantages : high build speed, low operating cost, large part size, no phase change, no need for support generation, and forth. However, those sheet deposition type rapid prototyping system require an additional post processing to remove excessive material attached to the part. This post processing is time consuming and labor intensive. Moreover, it is difficult for those system to fabricate parts with hollow cores and internal cavities. A new sheet deposition type rapid prototyping system that minimizes the post processing is proposed. The proposed system automatically removes excessive material in a peeling-off process between two cutting processes. In this way, the proposed system can reduce the post-processing time and cost as well as the limitation of the feasible geometric shapes in the conventional sheet deposition system.

• Journal of the Korea Institute of Military Science and Technology
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• v.16 no.4
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• pp.501-506
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• 2013

This research is to develop a simulation framework to gauge the mission effectiveness of the rotorcraft system that will be a part of Korean future combat system. The rotorcraft system comprises of many subsystems that are very time-consuming and tedious to model. Each subsystem and its characteristics have been modeled using component-based modeling techniques, which enhances its reusability. The entire system is then constructed from the individually modeled component, which significantly reduces the modelling time. The mission effectiveness of the rotorcraft system is simulated using the developed models, and the output indicates that the methodology proposed in this study is useful, which will be suitable for the modeling and simulation of Korean future combat system.