• Journal of the Korea Concrete Institute
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• v.20 no.6
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• pp.697-704
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• 2008

This paper presents an analytical prediction of Nonlinear characteristics of prestressed concrete bridges by strengthened of externally tendon considering construction sequence, using unbonded tendon element and beam-column element based on flexibility method. Unbonded tendon model can represent unbounded tendon behavior in concrete of PSC structures and it can deal with the prestressing transfer of posttensioned structures and calculate prestressed concrete structures more efficiently. This tendon model made up the several nodes and segment, therefore a real tendon of same geometry in the prestressed concrete structure can be simulated the one element. The beam-column element was developed with reinforced concrete material nonlinearities which are based on the smeared crack concept. The fiber hysteresis rule of beam-column element is derived from the uniaxial constitutive relations of concrete and reinforcing steel fibers. The formulation of beam-column element is based on flexibility. Beam-column element and unbonded tendon element were be involved in A computer program, named RCAHEST (Reinforced Concrete Analysis in Higher Evaluation System Technology), that were used the analysis of RC and PSC structures. The proposed numerical method for prestressed concrete structures by strengthened of externally tendon is verified by comparison with reliable experimental results.

• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.545-554
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• 2013

Plasmid isolation of kimchi-derived Weissella cibaria KLC140 revealed six different plasmids. The smallest plasmid, pKW2124, was DNA sequenced and characterized, showing 2,126 bp with a GC content of 36.39% and five putative open reading frames (ORFs). In silico analysis of these ORFs showed ORF1 encodes a putative replication protein similar to rolling circular replication proteins from other lactic acid bacteria. However, a single-stranded intermediate was not detected when S1 nuclease was treated, suggesting it may follow theta replication. Interestingly, the replication initiation site of this plasmid is 100% identical to other plasmids from lactic acid bacteria, suggesting it may function for replication initiation. To construct a surface layer expression vector, pTSLGFP, slpA encoding the surface layer protein from Lactobacillus acidophilus was PCR amplified and fused with the gfp gene, forming a SLGFP fused gene. The plasmid pKW2124 was cloned into the XbaI site of pUC19, forming an Weissella-E. coli shuttle vector pKUW22. NheI-linearized pTSLGFP was ligated into pKUWCAT containing pKUW22 and the chloramphenicol acetyltransferase gene from pEK104, resulting in an 8.6 kb pKWCSLGFP surface layer expression vector. After transformation of this vector into W. cibaria KLC140, a GFP fluorescence signal was detected on the surface of the transformant, substantiating production of SLGFP fused protein and its secretion. This is the first report for construction of a Weissella surface layer expression vector, which may be useful for surface layer production of beneficial proteins in Weissella.

• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.1
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• pp.43-49
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• 2002

We have cloned cDNAs encoding toxin from the spider, Araneus ventricosus, and constructed a recombinant baculovirus expressing the insecticidal toxin. The cDNAs encoding toxin were cloned from the cDNA library of A. ventricosus. Sequence analysis of the cDNAs encoding the toxin of A. ventricosus revealed that the 240 bp cDNA for AvTox-1 and 192 bp cDNA for AvTox-2 have an open reading frame of 80 and 64 amino acid residues, respectively. The deduced protein sequence of the toxin genes of AvTox-1 and AvTox-2 was aligned to that of the snack Anemonia sulcata and scorpion Centruroides limpidus limpidus, respectively. Northern blot analysis indicated that AvTox-2 toxin gene showed a fat body-spe-cific expression pattern at the transcriptional level. Furthermore, we have explored the possibility of improving baculovirus by incorporating the A. vontricosus toxin gene into Bombyx mori nuclear polyhedrosis virus genome under the control of polyhedrin promoter, The AvTox-2 toxin gene was expressed as approximately 5.8 kDa band in the recombinant baculovirus-injected silkworm larvae. Bioassays with the recombinant virus expressing AvTox-2 on 5th instar silkworm larvae demonstrated a decrease in the time to kill $(LT_{50} days)$ compared to wild-type BmNPV-Kl $(LT_{50} 6.72 days)$ in the injection of 10 viruses. These results indicate that A. ventricosus toxin is a novel member of the spider toxin family, suggesting that the toxin gene can be used in recombinant baculoviruses to reduce insect feeding damage and increase the speed of insect kill.

• Journal of Institute of Control, Robotics and Systems
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• v.11 no.10
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• pp.851-856
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• 2005

The DNA and protein data of diverse species have been daily discovered and deposited in the public archives according to each established format. Database systems in the public archives provide not only an easy-to-use, flexible interface to the public, but also in silico analysis tools of unidentified sequence data. Of such in silico analysis tools, multiple sequence alignment [1] methods relying on pairwise alignment and Smith-Waterman algorithm [2] enable us to identify unknown DNA, protein sequences or phylogenetic relation among several species. However, in the existing multiple alignment method as the number of sequences increases, the runtime increases exponentially. In order to remedy this problem, we adopted a parallel processing suffix tree algorithm that is able to search for common subsequences at one time without pairwise alignment. Also, the cross-matching subsequences triggering inexact-matching among the searched common subsequences might be produced. So, the cross-matching masking process was suggested in this paper. To identify the function of the clusters generated by suffix tree clustering, BLAST was combined with a clustering tool. Our clustering and annotating tool is summarized as the following steps: (1) construction of suffix tree; (2) masking of cross-matching pairs; (3) clustering of gene sequences and (4) annotating gene clusters by BLAST search. The system was successfully evaluated with 22 gene sequences in the pyrubate pathway of bacteria, clustering 7 clusters and finding out representative common subsequences of each cluster

• Journal of the Korea Concrete Institute
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• v.11 no.5
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• pp.21-31
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• 1999

The companion paper presents an analytical model to predict behaviors of PSC-Beam bridges according to continuity of spans. This paper aims at providing several examples of its application to PSC-Beam bridge. In this regard, many uncertainties affecting to the continuity of spans (such as the ultimate shrinkage strain of slab and girders, the prestressing creep of girders, and the time adopting prestressing force) are analysis in detail. Moreover, to increase the serviceability and to remove th inherent structural defects including the cracking at interior supports, a necessity for the parametric studies of PSC-Beam bridges reflecting the construction sequence is emphasized.

• Tunnel and Underground Space
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• v.6 no.4
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• pp.287-297
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• 1996

Input data play an important role in numerical analyses. However, it is difficult to obtain appropriate data for many reasons. In order to overcome those difficulties, an automatic MPBX(Multi-Position Borehole Extensometer) monitoring system is developed. This system can be applied to the analyses of measured data which include evaluation of the stabiliyt, efficiency of construction and the validity of numerical analyses. At first, the MPBX among field measurement tools is considered in this study. Also, 3-dimension finite element analyses are carried out to verify the influence of geological conditions and excavation sequence on the MPBX displacement. The effect of geological conditons on the displacement can be estimated qualitatively qualitavely from the measured data by plotting displacement vs. depth.

• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.837-842
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• 2013

A cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733 was isolated, characterized, and used for the construction of a cloning vector to engineer Leuconostoc species. pMBLR00 is a rolling circle replication plasmid, containing 3,370 base pairs. Sequence analysis revealed that pMBLR00 has 3 open reading frames: Cop (copy number control protein), Rep (replication protein), and Mob (mobilization protein). pMBLR00 replicates by rolling circle replication, which was confirmed by the presence of a conserved double-stranded origin and single-stranded DNA intermediates. An Escherichia coli-Leuconostoc shuttle vector, pMBLR02, was constructed and was able to replicate in Leuconostoc citreum 95. pMBLR02 could be a useful genetic tool for metabolic engineering and the genetic study of Leuconostoc species.

• Journal of Educational Research in Mathematics
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• v.8 no.2
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• pp.485-494
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• 1998

This study is on the theory of Piaget's reflective abstraction and the mechanism of the development of knowledge and the history of algebra and its application to understand the difficulties that many students have in learning algebra. Piaget considers the development of knowledge as a linear process. The stages in the construction of different forms of knowledge are sequential and each stage begins with reorganization. The reorganization consists of the projection onto a higher level from the lower level and the reflection which reconstructs and reorganizes within a lager system that is transferred by profection. Piaget shows that the mechanisms mediating transitions from one historical period to the next are analogous to those mediating the transition from one psychogenetic stage to the next and characterizes the mechanism as the intra, inter, trans sequence. The historical development of algebra is characterized by three periods, which are intra inter, transoperational. The analysis of the history of algebra by the mechanism explains why the difficulties that students have in learning algebra occur and shows that the roles of teachers are important to help students to overcome the difficulties.

• Plant Resources
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• v.8 no.3
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• pp.181-187
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• 2005

A cinnamoyl CoA reductase (CCR) cDNA (ClCCR) was isolated from tabroot mRNAs of Codonopsis lanceolata by cDNA library construction, and its expression was investigated in relation to abiotic stresses. The ClCCR is 1008 bp in length with an open reading frame (ORF) of 336 amino acids. The deduced amino acid sequence was showed high similarity with cinnamoyl-CoA reductases of P. tremuloides (AAF43141) 87%, F.${\times}$aranassa (AAP46143) 83%, L. album (CAD29427) 80%, E. gunnii (CAA66063) 72%, S. tuberosum (AAN71761) 83%. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was revealed that the ClCCR expression was regulated by abiotic stresses.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.04a
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• pp.66-66
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• 2003

The coleoptera is the most species-rich order of animals. Relatively little is known about Coleoptera genes and genome. We describe here the construction and DNA sequencing of cDNA libraries from Protaetia brevizarsis, a fruit tree pest in Korea. We sequenced and analyzed 3072 ESTs from wholebody of Protaetia brevizarsis larvae. (omitted)