• Title/Summary/Keyword: Conservation science

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Characteristic Analysis on Mixed Filler of Conservation Materials for Stone Cultural Heritage (석조문화재 보존처리에 사용되는 혼합충전제의 특성분석)

  • Song, Chi-Young;Han, Min-Su;Lee, Jang-Jon;Jun, Byung-Kyu;Do, Min-Hwan
    • Journal of Conservation Science
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    • v.25 no.4
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    • pp.439-450
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    • 2009

  • The purpose of this study is to find out characteristics of the fillers made by epoxy resin (L-30) and filling epoxy resin (L-50) with talc, quartz sand and wollastonite with different mixture ratio of 5%, 50%, 80%, 120%, and 150%. The viscometer and colorimeter were used to measure the viscosity and chromaticity of the fillers. Additionally, IC and SEM were used to reveal characteristics of the filler, and checked ultrasonic wave velocity, compressive strength and contact angle to estimate the stability between the filler and stones which are essential for conservation treatments. The filler mixed with the talc had the lowest value in the ultrasonic wave velocity analysis, and its compressive strength decreased as the mixing rate of talc increased. On the other hand, wollastonite had higher values than others in the ultrasonic wave velocity and the compressive strength regardless of epoxy resin type, also, these values increased as mixing rate increased. The properties of the filler, which include the granularity and shape, have influence on characteristics of the stone conservation adhesives. Thus, the filler type, characteristic, and mixture ratio must be considered for effective conservation treatment.

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The Conservation Treatment of the Central Asian Mural Painting(II) -An Investigation on the Pigments for the Mural Painting and of the Plants Used for Making the Original Wall - (중앙아세아벽화(中央亞細亞壁畵) 보존처리(保存處理)(II) - 壁畵(벽화)의 채색(彩色) 안료(顔料) 및 벽체(壁體) 조성(造成)에 사용(使用)된 초재류(草材類) 조사(調査) -)

  • Yi, Yonghee;Yu, Heisun;Kim, Soochul;Kang, Hyungtae;Jo, Yeontae;Aoki, Shigeo;Ohbayashi, Kentaro
    • Conservation Science in Museum
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    • v.4
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    • pp.1-16
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    • 2003

  • For the conservation treatment of the Central Asia mural painting which is to be exhibited in the new museum in Yongsan, we analyzed the pigments used in this mural painting and examined to identify the species of the straw in the wall. We also analyzed the species of the wood of the wooden protective frame and the material of the paper in it, in order to review the material and technique of the conservation treatment performed before the mural painting had been brought to the National Museum of Korea in 1916. The results were as follows: the black pigments of Bon4075 and Bon4078 is carbon(C); the white pigment on the background is gypsum[Ca(SO)4(H2O)2]; the red pigment is lead oxide(Pb3O4) and hematite(Fe2O3) etc. The straw, which had been mixed into the wall to prevent the wall from cracking, was proved to be either wheat straw or oats straw. The wooden protective frame, which protects the mural painting now, was proved to be made of Salix, Populus, Cryptomeria japonica and pine. The paper discovered in the frame was proved to be made of the bark of a mulberry.

  • https://doi.org/10.22790/conservation.2003.4.001 인용 PDF

Relationship among porcine lncRNA TCONS_00010987, miR-323, and leptin receptor based on dual luciferase reporter gene assays and expression patterns

  • Ding, Yueyun;Qian, Li;Wang, Li;Wu, Chaodong;Li, DengTao;Zhang, Xiaodong;Yin, Zongjun;Wang, Yuanlang;Zhang, Wei;Wu, Xudong;Ding, Jian;Yang, Min;Zhang, Liang;Shang, Jinnan;Wang, Chonglong;Gao, Yafei
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.2
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    • pp.219-229
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    • 2020

  • Objective: Considering the physiological and clinical importance of leptin receptor (LEPR) in regulating obesity and the fact that porcine LEPR expression is not known to be controlled by lncRNAs and miRNAs, we aim to characterize this gene as a potential target of SSC-miR-323 and the lncRNA TCONS_00010987. Methods: Bioinformatics analyses revealed that lncRNA TCONS_00010987 and LEPR have SSC-miR-323-binding sites and that LEPR might be a target of lncRNA TCONS_00010987 based on cis prediction. Wild-type and mutant TCONS_00010987-target sequence fragments and wild-type and mutant LEPR 3'-UTR fragments were generated and cloned into pmiRRB-REPORTTM-Control vectors to construct respective recombinant plasmids. HEK293T cells were co-transfected with the SSC-miR-323 mimics or a negative control with constructs harboring the corresponding binding sites and relative luciferase activities were determined. Tissue expression patterns of lncRNA TCONS_00010987, SSC-miR-323, and LEPR in Anqing six-end-white (AQ, the obese breed) and Large White (LW, the lean breed) pigs were detected by real-time quantitative polymerase chain reaction; backfat expression of LEPR protein was detected by western blotting. Results: Target gene fragments were successfully cloned, and the four recombinant vectors were constructed. Compared to the negative control, SSC-miR-323 mimics significantly inhibited luciferase activity from the wild-type TCONS_00010987-target sequence and wild-type LEPR-3'-UTR (p<0.01 for both) but not from the mutant TCONS_00010987-target sequence and mutant LEPR-3'-UTR (p>0.05 for both). Backfat expression levels of TCONS_00010987 and LEPR in AQ pigs were significantly higher than those in LW pigs (p<0.01), whereas levels of SSC-miR-323 in AQ pigs were significantly lower than those in LW pigs (p<0.05). LEPR protein levels in the backfat tissues of AQ pigs were markedly higher than those in LW pigs (p<0.01). Conclusion: LEPR is a potential target of SSC-miR-323, and TCONS_00010987 might act as a sponge for SSC-miR-323 to regulate LEPR expression.

  • https://doi.org/10.5713/ajas.19.0065 인용 PDF KSCI

정오표

  • Lee, Bo-Yeong;Yu, Hyo-Seon;Park, So-Hyeon
    • Journal of Conservation Science
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    • v.34 no.3
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    • pp.245-245
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    • 2018

  • PDF KSCI