• Title/Summary/Keyword: Congo

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Decolorization of a Sulfonated Azo Dye, Congo Red, by Staphylococcus sp. EY-3

  • PARK, EUN-HEE;JANG, MOON-SUN;CHA, IN-HO;CHOI, YONG-LARK;CHO, YOUNG-SU;KIM, CHEORL-HO;LEE, YOUNG-CHOON
    • Journal of Microbiology and Biotechnology
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    • v.15 no.1
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    • pp.221-225
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    • 2005
  • A Staphylococcus sp. EY-3 with the capability of decolorizing Congo Red was isolated from soil at an effluent treatment plant of a textile and dyeing industry. This strain was able to almost completely decolorize a high concentration of Congo Red in 48 h under aerobic conditions. Optimal color removal (more than 96%) was achieved at 30- 40oC, and no noticeable effects of different pH values (5.5- 8.0) on decolorization were observed. This strain also exhibited a remarkable decolorization capability against azo dyes under aerobic conditions, even at a high concentration (dyes 1 g/l) of dye. The metabolic product of Congo Red degradation by this strain was identified by gas chromatography with mass selective detection (GC/MSD) to be an amine derivative benzidine.

Comparison of Dyes for Easy Detection of Extracellular Cellulases in Fungi

  • Yoon, Ji-Hwan;Park, Ji-Eun;Suh, Dong-Yeon;Hong, Seung-Beom;Ko, Seung-Ju;Kim, Seong-Hwan
    • Mycobiology
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    • v.35 no.1
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    • pp.21-24
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    • 2007
  • To evaluate which dye is effective in a plate assay for detecting extracellular cellulase activity produced by fungi, four chromogenic dyes including remazol brilliant blue, phenol red, congo red, and tryphan blue, were compared using chromagepic media. For the comparison, 19 fungal species belonging to three phyla, ascomycota, basidiomycota, and zygomycota were inoculated onto yeast nitrogen-based media containing different carbon substrates such as cellulose (carboxylmethyl and avicel types) and cellobiose labeled with each of the four dyes. Overall, the formation of clear zone on agar media resulting from the degradation of the substrates by the enzymes secreted from the test fungi was most apparent with media containing congo red. The detection frequency of cellulase activity was also most high on congo red-supplemented media. The results of this study showed that congo red is better dye than other three dyes in, a plate assay for, fungal enzyme detection.

Effect of Chemical Structures of Congo Red and Benzopurpurine 4B on the Dyeing Property and Lightfastness of Cotton Fabric (Congo Red와 Benzopurpurine 4B의 화학구조가 면직물의 염색성 및 광퇴색성에 미치는 영향)

  • 이영희;박준명;김경환
    • Textile Coloration and Finishing
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    • v.2 no.1
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    • pp.8-13
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    • 1990
  • The fading of dyed material by light has long been subject of investigation, yet surprisingly little is known of the fundamental photochemical reactions, because of mainly the complex nature of dye-fibre system. The effect of the chemical structure of dye on lightfastness has been mostly studied when there is substituents on the place satisfied Hammett rule. Therefore, in this investigation the effectiveness of chemical structures of Congo Red and Benzopurpurine 4B unsatisfied Hammett rule on dyeing property and lightfastness of cotton was studied. The results obtained from this study were as follows; 1. Highly polar solvents showed hypsochromic shift. 2. Adsorption isotherm curves of the two dyes were Freundlich type. And Congo Red showed good dyeing property in comparision with Benzopurpurine 4B. 3. The type of the FR curve of the two dyes was first-ordr curve. And the CF curves of the two dyes showed normal fading state during irradiation. 4. During irradiation, the Hue was changed on the part of yellowish. The Value was increasing and the Chroma was decreasing. 5. Benzopurpurine 4B showed good lightfastness in comparision with Congo Red.

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Decoloration of Polycyclic Aromatic Dyes by Mushroom Fungi (버섯균에 의한 염료의 탈색)

  • Sancheti, Sandesh;Sancheti, Shruti;Seo, Sung-Yum
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.73-79
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    • 2009
  • As waste-water disposal plants and oxidative biodegradation for the removal of waste polyaromatic dyes are proved to be ineffective due to the chemical stability of dyes, we studied various strains of mushroom fungi for the removal of these dyes. 100 fungi were isolated from the mushroom samples of 230 species collected in Korea. The growth medium containing a dye (Bromophenol Blue, Congo Red, or Methylene Blue) was inoculated to 10% and incubated for 7 days without shaking. The six strains which removed dyes effectively were selected for further studies with respect to removal of polycyclic aromatic dyes. For all strains, the rate of decoloration of dyes was increasing with Methylene Blue, Bromophenol Blue and Congo Red. The rate of decoloration was higher with stationary culture than with shaking culture. Adsorption of the dyes was the highest with Congo Red.

Evaluation of Foliar Uptake of Eight Fungicides Using a New Measuring Tool, Congo Red Method

  • Yu, Ju-Hyun;Lim, He-Kyoung;Cho, Kwang-Yun;Kim, Jeong-Han
    • Journal of Applied Biological Chemistry
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    • v.44 no.1
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    • pp.27-31
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    • 2001
  • Using a new evaluation method, Congo red method, the foliar uptakes of eight fungicides were measured. Among the fungicides tested, fenarimol, myclobutanil, edifenphos, isoprothiolane, and tricyclazole were found to be penetrable (11.0~42.8%), and were continuously absorbed into the leaf regardless of their formulation types. Procymidone, dimethomorpb, and ethaboxan were seldomly absorbed into plant leaf. The uptakes of dimethomorph and ethaboxam were facilitated (18.1~22.7%) in the presence of Koremul-OE-20, as an activator, showing plateau phase at 12h after application. Foliar uptake of fenarimol and myclobutanil into cucumber plant by spray application was larger than that by dropping application, while observed opposite in the cases of dimethomorph and ethaboxam containing activator. Congo red method in conjunction with spraying application used in the present study produced the clear results on foliar uptake of fungicides and therefore proved to be better than the conventional method in which the dropping application of pesticide has been used.

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The Influence of Electrolytes on the Color Fastness Properties of Congo Red on Cotton Fabric (Congo Red로 염색한 면직물의 견뢰성에 미치는 전해질의 영향)

  • Lee, Young-Hee;Kim, Kwang-Oh;Park, Joon-Myung;Sung, Woo-Kyung;Kim, Kyung-Hwan
    • Textile Coloration and Finishing
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    • v.3 no.3
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    • pp.15-22
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    • 1991
  • The effects of electrolytes on color fastness properties of cotton fabric with Congo Red have been studied at $90^{\circ}C$. Each dyeing carried with $10 \times 10^{-6}M$ of Congo Red and with various concentration of electrolytes. The results obtained from this study were as follow 1. In the case of none, the fastness to washing became worse, but the fastness to light better with increasing dye concentration. 2. In the case of additing an electrolyte, the fastness to washing became worse and the fastness to light better than the case of none. The samples had become yellower after washing test. Yellowness had been reduced after fade-O-meter test. 3. What kind of electrolytes could not have any effect on the fastness to washing, but they influenced the fastness to light in the order of LiCl

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Decolorization of Dyehouse Effluent and Biodegradation of Congo Red by Bacillus thuringiensis RUN1

  • Olukanni, O.D.;Osuntoki, A.A.;Awotula, A.O.;Kalyani, D.C.;Gbenle, G.O.;Govindwar, S.P.
    • Journal of Microbiology and Biotechnology
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    • v.23 no.6
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    • pp.843-849
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    • 2013
  • A dye-decolorizing bacterium was isolated from a soil sample and identified as Bacillus thuringiensis using 16S rRNA sequencing. The bacterium was able to decolorize three different textile dyes, namely, Reactive blue 13, Reactive red 58, and Reactive yellow 42, and a real dyehouse effluent up to 80-95% within 6 h. Some non-textile industrially important dyes were also decolorized to different extents. Fourier transform infrared spectroscopy and gas chromatography-mass spectrometer analysis of the ethyl acetate extract of Congo red dye and its metabolites showed that the bacterium could degrade it by the asymmetric cleavage of the azo bonds to yield sodium (4-amino-3-diazenylnaphthalene-1-sulfonate) and phenylbenzene. Sodium (4-amino-3-diazenylnaphthalene-1-sulfonate) was further oxidized by the ortho-cleavage pathway to yield 2-(1-amino-2-diazenyl-2-formylvinyl) benzoic acid. There was induction of the activities of laccase and azoreductase during the decolorization of Congo red, which suggests their probable role in the biodegradation. B. thuringiensis was found to be versatile and could be used for industrial effluent biodegradation.

Combination of Colony Formation and Congo Red Reaction for Detecting Intra- and Extra-Cellular Cellulolytic Activities (세포내외 섬유소 분해능 검출을 위한 Colony 형성과 Congo Red반응의 병용)

  • Lee, D.S.;Kim, H.K.;Pack, M.Y.
    • Microbiology and Biotechnology Letters
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    • v.12 no.4
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    • pp.305-309
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    • 1984
  • A selective medium which allows growth of only cellulolytic bacteria was developed. The medium composed of 0.5% carboxymethylcellulose (CMC), 0.005% yeast extract, minerals and agar. Colony formation on this medium indicates overall activities of cellulose utilization. A subsequent test with Congo Red dye could distinguish extracellular cellulolysis from intracellular type.

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Staining Response of KMnO4-Treated Animal Amyloid Proteins to Congo red (과망간산카리움 처리(處理)한 동물(動物) 아밀로이드 단백(蛋白)의 Congo red에 대한 염색반응(染色反應))

  • Kim, Duck Hwan
    • Korean Journal of Agricultural Science
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    • v.13 no.1
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    • pp.139-146
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    • 1986
  • The characteristics of the amyloid proteins were histochemically investigated with the amyloidladen organs from bovine(15 cases), canine(1 case), feline(1 case), and artificially induced rabbit amyloidosis(1 case). The amyloid-laden organs from bovine, canine and experimental rabbit amyloidosis showed potassium-permanganate-senstive reaction, revealing complete disappearance for Congo red affinity, loss of brick-red colored fluorescence and green birefringence. From these findings, the amyloids from bovine, canine and experimentally induced rabbit amyloidosis were thought to be equivalent to amyloid protein A (AA protein). In the present feline case with amyloidosis, however, the amyloid proteins revealed potassium permanganate-resistant reaction, showing unchanged affinity for Congo red, and immunoglobulin was also deposited in the glomeruli of the kidney. From these findings, the amyloid proteins from feline case with amyloidosis were considered to be equivalent to amyloid light chain-related proteins (AL protein).

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West African Pregnancy Experienced Women's Self-Rated Health and Related Factors: Case of Democratic Republic of the Congo (서아프리카 임신경험 여성들의 주관적 건강상태와 관련 요인: 콩고민주공화국 사례를 중심으로)

  • Lee, Hyeong Il;Nam, Eun Woo;Song, Jin Sung
    • Health Policy and Management
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    • v.24 no.1
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    • pp.85-91
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    • 2014
  • Background: This study aims to analyze West African pregnancy experienced women's self-rated health and related factors in Democratic Republic (DR) of the Congo, and provide basic data which can be used in developing maternal and child heath projects and health promotion programs to enhance women's self-rated health in the DR Congo. Methods: A survey was conducted among 400 women, 300 of whom were from Kenge and 100 from Boko in Bandundu Province. Three hundred and fifty-two questionnaires were administered on factors expected to affect the self-rated health, categorized into basic information and economic status of respondents, healthcare service, and illness patterns. The hierarchical logistic regression analysis was performed taking each variable into account. Results: The results show that those who have had nutrition education, work in the office, have no health insurance, or have had no illness for the last one year perceive themselves to be in good health. Conclusion: To improve the self-rated health status and promote maternal and child health in DR Congo, the results suggest that the government or relevant agencies should develop programs to educate people regarding health and nutrition, and policies based on the right status of each country. Systems are also required for healthcare at all social levels along with aspects to avoid illness in daily life.