• 생명과학회지
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• 제13권2호
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• pp.143-149
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• 2003

This experiment was undertaken to investigate proper conditions for protoplast isolation and regeneration from the mycelia of Lyophyllum ulmnrium. Protoplast isolation and regeneration are influenced by a variety of factors such as enzyme, osmotic stabilizer, reaction time and age of mycelia. A combination of Novozyme 234 (10mg/ml) and cellulase Onozuka R-10 (10 mg/ml) with 0.6 M $MgSO_4$ was most effective for isolation of the protoplasts. The optimum reaction time of the mycelia with the lytic enzymes was 3.5~4 hours at $28^{\circ}C$ in shaking condition at 120 strokes per min. High yield of the protoplasts were obtained from its 4~5 days old mycelia on complete agar media. Its protoplasts were regenerated to normal hyphae. Regeneration media with 0.6 M sucrose were proper for regeneration of the protoplasts. Their regeneration frequency on complete agar media was 2.3~2.7%.

• Structural Engineering and Mechanics
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• 제51권2호
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• pp.277-297
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• 2014

Since the isolation bearings undergo large displacements in base-isolated structures, impact with adjacent structures is inevitable. Therefore, in this investigation, the effect of impact on seismic response of isolated structures mounted on double concave friction pendulum (DCFP) bearings subjected to near field ground motions is considered. A non-linear viscoelastic model of collision is used to simulate structural pounding more accurately. 2-, 4- and 8-story base-isolated buildings adjacent to fixed-base structures are modeled and the coupled differential equations of motion related to these isolated systems are solved in the MATLAB environment using the SIMULINK toolbox. The variation of seismic responses such as base shear, displacement in the isolation system and superstructure (top floor) is computed to study the impact condition. Also, the effects of variation of system parameters: isolation period, superstructure period, size of seismic gap between two structures, radius of curvature of the sliding surface and friction coefficient of isolator are contemplated in this study. It is concluded that the normalized base shear, bearing and top floor displacement increase due to impact with adjacent structure. When the distance between two structures decreases, the base shear and displacement increase comparing to no impact condition. Besides, the increase in friction coefficient difference also causes the normalized base shear and displacement in isolation system and superstructure increase in comparison with bi-linear hysteretic behavior of base isolation system. Totally, the comparison of results indicates that the changes in values of friction coefficient have more significant effects on 2-story building than 4- and 8-story buildings.

• 한국지반공학회논문집
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• 제34권1호
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• pp.5-16
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• 2018

본 연구에서는 지반에서 1차적인 면진작용을 할 수 있는 지반형 지진격리장치의 내진성능을 평가하였다. 테프론과 제강슬래그를 이용하여 지반형 지진격리장치를 조성한 후 그 위에 모형 상부구조물을 설치하고 1-G 진동대 실험을 수행하였다. 다양한 수준의 입력지진파에 대해 응답가속도와 응답스펙트럼을 분석하였으며, 지진격리장치가 없는 고정기초 구조물과 결과를 비교하였다. 연구결과, 제강슬래그형 지진격리장치가 가장 가속도 저감효과가 좋았으며, 테프론형 지진격리장치는, 중 약진 조건에서는 가속도 저감효과가 크게 없고 강진조건에서는 가속도 저감효과가 좋았다. 입력파가 상부질량(Mass)으로 전달되면서, 고정기초 구조물의 응답스펙트럼은 입력지진파에 비해 단주기영역에서 증폭하고, 테프론과 제강슬래그를 이용한 지진격리장치가 있는 구조물의 응답스펙트럼은 입력지진파에 비해 장주기 영역에서 증폭하였다. 이러한 주기특성 변화와 재료간의 마찰특성이 가속도 저감효과에 영향을 준 것으로 판단된다.

• 대한의생명과학회지
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• 제20권3호
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• pp.180-184
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• 2014

Mitochondria play a crucial role in many essential biological events by way of the electron transport chains and intermembrane proteins that they contain. Abnormalities in the mitochondria are strongly correlated with the development of diseases such as atherosclerosis, cancer, and diabetes. However, the study of mitochondria has been referred to as 'labor-intensive' because of the difficulty in isolating the organelles from their various sources, which can include cultured cells and tissues. Multiple companies provide mitochondria isolation kits, and it is possible for investigators to use different kits and apply different protocols depending on the source of the mitochondria. Therefore, we focused on producing an isolation buffer that could be applied to both cultured cells and tissues, and optimized an isolation protocol that could be used with both. Specifically, we adjusted the buffer condition that can be applied to human cervical cancer cells, fibroblasts, and tissues such as mouse liver and spleen. We also optimized the protocol to improve the efficacy and efficiency of the steps involved in the isolation of mitochondria. These methodological improvements may contribute to advanced research by allowing investigators to overcome the difficulties involved in isolation of mitochondria from biological samples.

• 한국정보통신학회논문지
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• 제26권10호
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• pp.1567-1570
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• 2022

This paper, we presents a RF protection technique of antenna switch by improving the power handling capability in worst case environment mode for mobile phone applications without critical payment of circuit performances such as insertion loss, isolation and ACBV (AC breakdown voltage). By applying a additional capacitive path located in front of the antenna in cell-phone, it performs the effective reduction of input power in high voltage standing wave ratio (VSWR) condition. Under the all-path off condition which causes a high VSWR, it achieved 37.7dBm power handling level as high as 5.7dB compared to that of conventional one at 2GHz. In addition, insertion loss and isolation performances were 0.31dB and 42.72dB at 2 GHz, respectively which were almost similar to that of the conventional circuit. The proposed antenna switch was fabricated in 130nm CMOS SOI technology.

• 대한전기학회논문지:시스템및제어부문D
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• 제54권2호
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• pp.81-86
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• 2005

In this paper we consider an optimal configuration problem for redundant inertial sensors which have uncertainty such as misalignment, scale factor error. The optimal configuration problem is treated from the viewpoint of navigation accuracy. We propose a necessary and sufficient condition for the optimal configuration of redundant sensors with no uncertainty, and a sufficient condition for the optimal configuration of redundant sensors with uncertainty. Finally we propose a condition for the optimal configuration based both navigation performance and FDI(fault detection and isolation).

• KSBB Journal
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• 제14권1호
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• pp.71-75
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• 1999

인 축적능이 우수한 균주를 자연계로부터 위노그라드스키 칼럼을 이용해 분리하여 Acinetobacter 속으로 동정하였고, Aci-netobacter CW3로 망명하였다. 인 축적균주의 배양초기조건은 $20^{\circ}C$. pH 7, 200rpm, 18.5mg $FO_4-P/\ell$가 최적임을 보였다. Acinetobacter CW3은 회분배양에서는 최적조건에서 12시간만에 인의 제거가 완료되었다. 이 균주는 호기조건에서는 인을 균주 내부에 과량 축적하고 첨가조건에서 인을 다시 방출함을 보였다.

• 한국정밀공학회지
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• 제21권10호
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• pp.137-146
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• 2004

This is the second of two companion papers concerned with the analysis and design of a pneumatic vibration isolation system. The properties of the system are clarified by observation of the transmissibility surface calculated by the models and algorithm developed in the first paper of this research. It Is shown that the nonlinear model proposed in this research is more closer to experimental results than the linear model that have been used in previous studies. The design optimization of the major design variables that affect the performance of the system is achieved by using the condition for attenuation, disturbance rejection and maximum damping in resonance peak. The design space search method is adopted for the optimization of the orifice area. The models, transmissibility calculation algorithms and design optimization techniques developed in this research are shown to be greatly helpful to the optimal design of the pneumatic vibration isolation system by experiment.

• 한국태양에너지학회 논문집
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• 제31권5호
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• pp.33-40
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• 2011

In this study, it is quantitatively analyzed for thermal Characteristics of isolation-heat paint which has been supplied to a domestic market through experiments and simulations. In the case of experiment using the container box, it shows 8% decrease on cooling energy consumption in a summer season, but shows increase on heating energy consumption. As a result, the analysis has found increase of energy consumption. As a result of holding simulations with meteorological data of domestic major cities, it shows an increase of energy consumption in domestic condition, and it is hard to expect an energy saving from the isolation-heat paint unless greater air-conditioning load.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제23권4호
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• pp.67-75
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• 2017

Purpose: This study is for the facility eligibility and improvement evaluation analysis for the patient care environment of 7 facilities which has national inpatient isolation units. Methods: For the evaluation, first of all, the patient care environment of national inpatient isolation units are scrutinized by the checklist which is used on from 2014 with the 4 fields of criteria : architectural planning and layout, general requirement and condition for operating the negative pressured isolated patient room, HVAC system, and waste water discharging system. Finally, the evaluation results are compared with that of 2014. Results: The result shows that the average value of facility eligibility is 89.3 percent and which is 8.9 percent higher than the value before the MERS occurred. Implications: It is clear that facility remodeling of 7 institutions is being performed continuously, and the result of facility eligibility evaluation is reflected on design of a new 21 national inpatient isolation units those are going to be constructed from 2016. Therefore, it is expected that this study is used as a practical reference to establish the criteria of patient care environment management and safety management for both infectious disease outbreak and general service condition.