• Title/Summary/Keyword: Condition of isolation

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Experimental study of reverberation time in ship's public area (선박의 공용구역 잔향시간의 실험적 연구)

  • Kim, Taemoo;Choi, Choongyoung;Park, Nojun;Park, JeanHyung;Kwun, Hyuk
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2013.04a
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    • pp.242-242
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    • 2013
  • Recently, importance of working environment is increased in commercial vessel and offshore structure. Marine facility design and ambient environment condition are influenced to enhancing human performance and reducing human errors. Consequently, the quality of accommodation where offshore facility crews sleep, eat and relax will influence their job performance and overall sense of comport and well-being. Therefore, adequate acoustic isolation between adjacent spaces is normally required to achieve satisfactory internal noise levels, acoustic privacy and speech intelligibility. In this study, the reverberation time is investigated in the public areas where there are not provided the information of material's noise reduction coefficient (NRC). The experiment of reverberation time is rarely performed for the various type public areas in a marine structure. Therefore, the reverberation time in a vessel is investigate in order to evaluate the room's noise reduction coefficient (NRC) in a public area.

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Isolation of pseudomonas sp. S-47 and its degradation of 4-chlorobenzoic acid

  • Seo, Dong-In;Lim, Jai-Yun;Kim, Young-Chang;Min, Kyung-Hee;Kim, Chi-Kyung
    • Journal of Microbiology
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    • v.35 no.3
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    • pp.188-192
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    • 1997
  • The strain of S-47 degrading 4-chlorobenzoic acid (4CBA) was isolated from Ulsan chemical industrial complex by enrichment cultivation with 1 mM 4CBA. The strain was Gram-negative rod and grew optimally at 30.deg.C and pH 7 under aerobic condition, so that the organism was identified as a species of Pseudomonas. Pseudomonas sp. S-47 degraded 4-chlorobenzoic acid to produce a yellow-colored meta-cleavage product, which was confirmed to be 5-chloro-2-hydroxymuconic semialdehyde (5C-2HMS) by UV-visible spectrophotometry. 5C-3HMS was proved trometry. This means that Pseudomonas sp. S-47 degraded 4CBA via 4-chlorocatechol to 5C-2HMS by meta-cleavage reaction and then to 5C-2HMA by 5C-2HMS dehydrogenase.

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Analysis and simulation of PT ferroresonance (PT철공진의 원인분석 및 현상모의)

  • Kang, Yong-Cheol;Lee, Byung-Eun;Zheng, Tai-Ying;Jang, Sung-Il;Kim, Yong-Gyun
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.42_43
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    • 2009
  • Ferroresonance is a resonance condition between a nonlinear iron core of a potential transformer (PT) and a capacitance. It can lead to PT voltages several times the normal equipment ratings, and cause the isolation broken and damage to equipments near the PT. This paper proposes a analysis method of PT ferroresonance in the time domain. Based on the simplified equivalent circuit, a differential equation was found and the flux was calculated when ferroresonance generates. The forced response and natural response were also analyzed. The performance of proposed analysis method was verified with the EMTP-RV generated data. The method can help analysis chaotic ferroresonance and periodic ferroresonance.

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Isolation, Identification and Optimal Cultrul Condition of Antioxidant Producing Bacterium Isolated from the Marine Sources

  • Kim, Man-Chul;Heo, Moon-Soo
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.343-346
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    • 2005
  • The isolated strain, SC2-1 was Gram-positive, catalase positive, facultatively anaerobic, oxidase negative, motile and small rods. The strain utilized sucrose, dextrose, fructose, mannitol and maltose as a sole carbon and energy source and sodium chloride required for the bacteria growth. The radical scavenging activity of the culture supernatants was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. This bacterium was identified based on cellular fatty acids analysis and 16S rDNA sequencing then named Exiguobacterium sp. SC2-1. The optimum culture conditions for production of antioxidant were $25^{\circ}C,$ pH 7.8 and NaCl concentration were 4%. The modified optimal medium compositions were maltose 2.5% (w/v), yeast extract 1.5% (w/v) and $KH_2PO_4$ 0.05% (w/v). Free radical scavenging activity of under optimal culture conditions were 93%.

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효모 세포벽 분해효소 생산균의 탐색 및 효소생산 최적조건의 조사

  • Cha, Seong-Kwan;Choi, Hea-Suk;Kim, Wang-June;Yoon, Suk-Hoo;Kim, Young-Bae
    • Microbiology and Biotechnology Letters
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    • v.24 no.2
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    • pp.143-148
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    • 1996
  • Thousand actinomycetes and 50 soil samples were used for the isolation of microorganisms producing yeast cell wall lytic enzymes. Among 493 strains producing large clear zones on autolysed washed yeast (AWY), 117 strains were selected on living yeast cell agar plates. With the method of lytic activity, one strain (St-1702) was selected, which was temporarily identified as Streptomyces eurythermus. The optimal condition for enzyme production of this strain was partially determined as follows: incubation of the strain for 3 days at 30$\circ$C in the medium containing 2% freeze dried yeast cell, 1% glucose, 1% K$_{2}$HPO$_{4}$, 0.01% MgSO$_{4}$'7H$_{2}$O, 0.5% peptone, and 0.2% (NH$_{4}$)$_{2}$CO$_{3}$ with pH 7.0. The protoplast formation of yeast by using the enzyme produced by this strain was compared with commercial enzymes.

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Development of Conical Rubber Mount using Compression and Shear Elasticity (압축 및 전단탄성을 이용한 원형 방진 고무 마운트 개발)

  • 김종연;권오병;김영구;김영중
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2002.05a
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    • pp.393-398
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    • 2002
  • Rubber isolator has properties that can adjust easily stiffness and can be formed various shape. Also, it has high damping and is effective about structure-borne noise at high frequency range, So, rubber mount has widely used to isolate vibration at industrial equipment and construction field. However, rubber material is nonlinear and require enough consideration about shape factor whenever it is designed. The purpose of this paper is to develop conical rubber mount using compression and shear elasticity. The first, the dimension of mount is calculated by theoretical analysis considering design condition and static characteristics have been analyzed by FEM method. In addition, the fatigue test of rubber mount is performed to get reliability for product life and dynamic stiffness test is executed to get dynamic magnification factor. Finally, transmissibility test of vibration isolator has been carried out to suggest normal quantity data about vibration isolation.

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Isolation of Sulfotransferase Producing Bacteria from Mouse Intestinal Microflora (생쥐의 장내미생물로부터 새로운 슬포트란스훼라제 생산균의 분리)

  • Kim, Byung-Taek;Kim, Eun-Ha;Kim, Dong-Hyun
    • YAKHAK HOEJI
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    • v.36 no.5
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    • pp.455-459
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    • 1992
  • Haemophilus K-12 producing novel sulfotransferase was isolated from mouce intestinal flora. The enzyme catalyzed the transfer of sulfate group from p-nitrophenylsulfate to phenolic compounds. The optimum medium condition for the sulfotransferase production was 0.2% sucrose, 1% yeast extract, $Na_{2}HPO_4$ and 0.5% NaCl. The enzyme production was induced by donor substrate, but was not by accepters. When p-nitrophenylsulfate was used as a donor substrate, 1-naphthol was best substrate, followed by phenol, p-acetaminophenol and tyramine.

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Study for Fault Diagnosis Methodologies Using Diagnosis for Monopropellant Propulsion System (단일 추진시스템 진단을 통한 고장진단 방법론에 관한 연구)

  • Song, Chang-Hwan;Lee, Young-Jin;Ku, Kyung-Wan;Lee, Kwon-Soon
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.2041_2042
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    • 2009
  • The diagnostic/prognostic problems for condition based maintenance or Prognostics and Health Management has been used. Primary objectives of diagnosis/prognosis are maximizing system availability and minimizing downtime from fault isolation through more effective troubleshooting efforts. Diagnosis aims to detect the onset of failures to improve system performance and reduce life cycle cost by reducing the failure time. The prognosis can reduce operational and support total ownership cost and improve safety of machinery and complex systems. In this Paper, a fault diagnosis methodology has been described using a monopropellant propulsion system model as a test bench.

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Isolation of Higher Alcohol-Producing Yeast as the Flavor Components and Determination of Optimal Culture Conditions

  • Kwon, Dong-Jin;Kim, Wang-June
    • Food Science and Biotechnology
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    • v.14 no.5
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    • pp.576-580
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    • 2005
  • Ten yeast strains affecting doenjang flavor were isolated from soybean fermented foods (traditional meju and doenjang), among which Zygosaccharomyces sp. Y-2-5, showing excellent growth, glucose consumption, pH, and flavor production, was selected. Higher alcohols produced by Zygosaccharomyces sp. Y-2-5 related to flavor were 2-propanol, 1-propanol, 2-methyl-1-propanol, 1-butanol, and 3.3-dimethyl-2-butanol. Optimal culture conditions for Zygosaccharomyces sp. Y-2-5 were 10% (w/v) NaCl, pH 4.0, 3.0% (w/v) glucose concentration, and inoculation time day 0 or 15 doenjang fermentation.

Isolation of Bacillus sp. AIR-5 PRoducing Maltopentaose Forming Amylase and Optimization of Maltopentaose Production (Maltopentaose 생산 균의 분리 및 생산 조건 연구)

  • ;;;;;Atsuo Kimura
    • KSBB Journal
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    • v.16 no.3
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    • pp.246-252
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    • 2001
  • We isolated a bacterium that produces an extracellular maltopentaose(G5)-forming amylase from amylose and soluble starch. The bacterium was identified and assigned as a Bacillus sp. AIR-5. The amylase did not hydrolyze maltose, maltotriose, maltotetraose or maltopentaose. Optimum medium composition for maltopentaose production in flask culture was 2%(w/v) soluble starch, 0.4%(w/v) tryptone, 0.5%(w/v) NaCl, 0.5%(w/v) K$_2$HPO$_4$, and 3 mM CaCl$_2$at pH 8.0, 28$^{\circ}C$. The highest yield for maltopentaose production in this condition was 6.45 g/L and was 32.55% of theoretical yield.

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