• Journal of Life Science
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• v.4 no.4
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• pp.170-175
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• 1994

The glucose transport across the mammalian plasma membranes is carried out by members of two distinct gene families, $Na^+$/glucose to transporter (SGLT) and glucose transporters (GLUTs). The energy requiring SGLT utilizes the sodium gradient to transport glucose and galactose against the concentration gradient. The energy independent transport (Facilitative transport) of glucose down the concentration gradient is mediated by the members of GLUTs. The facilitated transport of glucose is saturable, sterospecific and bidirectional across the membrane. To date, 6 kinds of isoforms of facilitative glucose transporters are found. These proteins are expressed in a tissue and cell specific manner, and shows distinct properties that reflect their specific functional roles.

• Journal of Mechanical Science and Technology
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• v.16 no.12
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• pp.1710-1718
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• 2002

The effect of fuel concentration gradient on the propagation characteristics of tribrachial (or triple) flames has been investigated experimentally in both two-dimensional and axisymmetric counterflows. The gradient at the stoichiometric location was controlled by the equivalence ratios at the two nozzles; one of which is maintained rich, while the other lean. Results show that the displacement speed of tribrachial flames in the two-dimensional counterflow decreases with fuel concentration gradient and has much larger speed than the maximum speed predicted previously in two-dimensional mixing layers. From an analogy with premixed flame propagation, this excessively large displacement speed can be attributed to the flame propagation with respect to burnt gas. Corresponding maximum speed in the limit of small mixture fraction gradient was estimated and the curvefit of the experimental data substantiates this limiting speed. As mixture fraction gradient approaches zero, a transition occurs, such that the propagation speed of tribrachial flame approaches stoichiometric laminar burning velocity with respect to burnt gas. Similar results have been obtained for tribrachial flames propagating in axisymmetric counterflow.

• Proceedings of the KSME Conference
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• 2000.04b
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• pp.422-427
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• 2000

Propagation characteristics of tribrachial flames have been investigated experimentally in both two-dimensional and axisymmetric counterflows. Mixture fraction gradient at stoichiometric location is controlled by varying equivalence ratios at the two nozzles, one of which maintains rich while the other lean premixture. Tribrachial flames propagating through these mixtures are investigated. The propagation speed of tribrachial flames in two-dimensional counterflow decreases with fuel concentration gradient and has much higher speed than the maximum speed predicted previously in two-dimensional mixing layers. From an analogy with premixed flame propagation, this excessively large propagation speed can be attributed to the tribrachial flame propagating with respect to burnt gas. Corresponding maximum speed in the limit of small mixture fraction gradient is estimated and extrapolated experimental results substantiate this limiting speed. As mixture fraction gradient approaches zero, a transition in propagation characteristics occurs, such that the propagation speed of tribrachial flame approaches stoichiometric laminar burning velocity with respect to burnt gas. Similar behavior has been obtained for tribrachial flames propagating in axisymmetric counterflow.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.12
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• pp.1115-1122
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• 2008

This paper presents a new microchip which can separate motile sperm by chemotaxis. The microchip was developed to create longitudinal concentration gradient in the microchannel due to diffusion. Linearly good concentration gradient of chemoattractant was generated without any fluid control devices. In sperm separation experiment with the developed microchip, mouse sperm was used as sample and acetylcholine was selected as chemoattractant. Human tubal fluid (HTF), buffer solution, was introduced into the microchannel of the microchip and attractants diluted in ratio of 1, 1/2, 1/4, 1/8, 1/16, 1/32 and 1/64 including control (DI water) were dropped in each outlet by $2\;{\mu}l$ volume with micropippet. After 5min, $1\;{\mu}l$ sperm solution was dropped into inlet of the chip. After 10 min, when sperms reached to the outlet by chemotaxis, we counted sperms in each outlet by using microscopy. Consequently, we could separate progressive motile sperm with the new microchip. In the experiment, the most sperms were isolated at the outlet dropped with 1/16 diluted solution. The optimal concentration gradient to induce chemotaxis was about 0.625 mg/ml/mm.

• Journal of Korean Society of Water and Wastewater
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• v.20 no.3
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• pp.443-450
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• 2006

For understanding sludge concentration profile as a function of time, sludge was sampled at each sampling port. When sludge concentration was 3g/L, the vertical sludge concentration distribution was similar to that of 2g/L of sludge concentration. During the early stage of sludge settling, sludge concentration increased remarkably as the sludge interface height in batch column became lower. The higher sludge concentration became, the worse sludge setteability became. Also, the type of sludge settling was influenced with sludge concentration gradient in batch column. In the same concentration, the greater sludge concentration gradient was, the faster sludge interface settled down. And the changing sludge concentrations in a batch settling or a continuous settling were simulated by using the equation of sludge interface height change model.

• Journal of Korean Society of Water and Wastewater
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• v.35 no.6
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• pp.507-516
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• 2021

In this study, the machine learning which has been widely used in prediction algorithms recently was used. the research point was the CD(chudong) point which was a representative point of Daecheong Lake. Chlorophyll-a(Chl-a) concentration was used as a target variable for algae prediction. to predict the Chl-a concentration, a data set of water quality and quantity factors was consisted. we performed algorithms about random forest and gradient boosting with Python. to perform the algorithms, at first the correlation analysis between Chl-a and water quality and quantity data was studied. we extracted ten factors of high importance for water quality and quantity data. as a result of the algorithm performance index, the gradient boosting showed that RMSE was 2.72 mg/m³ and MSE was 7.40 mg/m³ and R² was 0.66. as a result of the residual analysis, the analysis result of gradient boosting was excellent. as a result of the algorithm execution, the gradient boosting algorithm was excellent. the gradient boosting algorithm was also excellent with 2.44 mg/m³ of RMSE in the machine learning hyperparameter adjustment result.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.5 no.2
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• pp.111-121
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• 1993

Double-diffusive convection during solidification process of the binary mixture was studied numerically. Enthalpy method and finite element method were implemented in the analysis. Calculation carried out for $R{\alpha}_T=10^3-10^4$ and $R{\alpha}_T=0-10^5$. The results show that the variation of thermal Rayleigh number changes the fields of velocity, temperature and concentration, but the variation of solutal Rayleigh number gives little effects on those. In conclusion, concentration gradient can be negligible compared with temperature gradient in macroscopic point of view, although concentration gradient plays a role in forming dendrite.

• 한국연소학회:학술대회논문집
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• 2007.05a
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• pp.13-18
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• 2007

When triple flames propagated in a diverging channel, the effects of fuel dilution on the lift-off characteristics of triple flames were investigated. A multi-slot burner was used to stabilize the lift-off flame especially at weak fuel concentration gradients. It was reported that there is a maximum propagation velocity at a critical concentration gradient in an open jet regardless of fuel dilution. The enhancement of a diffusion flame affected to increase the propagation velocity around critical concentration gradients. However, the influence of a confined channel on the structure of triple flames according to fuel dilution needs to be investigated compared with an open jet case. This study aimed to examine the effect of a confined channel on the structure and the propagation velocity of the triple flames according to fuel dilution. Lift-off height and propagation velocity of triple flames were investigated by employing three kinds of fuel compositions diluted by nitrogen (0%, 25%, 50% $N_2$), Fuel dilution reduced the propagation velocity of triple flame in a confined channel mainly due to the decrease of flame temperature in premixed branch. Despite the difference in fuel dilution, the propagation velocity has a maximum value at a specific fuel concentration gradient even though the critical concentration gradient increases with fuel dilution. And the critical concentration gradient in a confined channel is larger than that in an open jet due to enhancement of convective diffusion.

• Korean Journal of Animal Reproduction
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• v.12 no.3
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• pp.141-147
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• 1988

These experiments were carried out to develop new techniques for in vitro separation of x-and Y-bearing spermatozoa. The results obtained in these experiments were summarized as follows: 1. Following centrifugation of discontinuous percoll density gradient, populatin of spermatozoa increased progressively from low to high density. The highest concentration of spermatozoa was observed at the 4th fraction which included 36.6% of spermatozoa. 2. As increasing percoll concentration, the higher motility index was obtained and the highest motility index(74.2) was obtained at the 5th fraction. 3. The percentage of B-body bearing spermatozoa following percoll density gradient centrifugation was decreased from 39.7% to 25.6%. 4. The sperm population following chromatography by sephadex gel and percoll density gradient centrifugation was decreased in 1st, 5th and 6th fractions but the reverse was turn for 2nd, 3rd and 7th fractions, and the highest sperm concentration was observed at the 7th fraction which included 37.4% of spermatozoa. 5. Motility index of spermatozoa was increased from 77.6 to 79.4 after the sephadex gel filtration, however it was decreased at all fractions after percoll density gradient centrifugation. The lowest motility index(33.2) was obtained from the 7th fraction. 6. The rate of B-body bearing spermatozoa was shown the trend to decrease by the sephadex gel filtration and the trend was accelerated by the percoll density gradient centrifugation. The lowest percentage of B-body bearing spermatozoa, 12.0% was obtained from the 5th fraction.

• Bulletin of the Korean Chemical Society
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• v.35 no.2
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• pp.365-370
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• 2014

For real-time monitoring of colloidal nanoparticles (NPs) in aqueous media, a light sheet type dark-field microscopy system combined with a microfluidic concentration gradient generator (${\mu}FCGG$-LSDFM) was developed. Various concentrations of colloidal Au NPs were simultaneously generated with the iFCGG and characterized with the LSDFM setup. The number concentrations and hydrodynamic size distributions were measured via particle counting and tracking analysis (PCA and PTA, respectively) approaches. For the 30 nm Au NPs used in this study, the lower detection limit of the LSDFM setup was 3.6 ng/mL, which is about 400 times better than that of optical density measurements under the same ${\mu}FCGG$ system. Additionally, the hydrodynamic diameter distribution of Au NPs was estimated as $39.7{\pm}12.2nm$ with the PTA approach, which agrees well with DLS measurement as well as the manufacturer's specification. We propose this ${\mu}FCGG$-LSDFM setup with features of automatic generation of NP concentration gradient and real-time monitoring of their physicochemical characteristics (e.g., number concentration, and hydrodynamic size distribution) as an important component of future high-throughput screening or high-content analysis platforms of nanotoxicity.