• 대한원격탐사학회지
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• 제38권6_1호
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• pp.1191-1205
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• 2022

복잡하고 광범위한 원인을 가진 대기오염물질 중 particulate matter (PM)은 입자의 크기에 따라 분류된다. 그 중 PM_2.5는 그 크기가 매우 작아 사람이 흡입하면 인간의 호흡기나 심혈관에 질병을 유발할 수 있다. 이러한 위험에 대비하기 위해서는 국가 중심의 관리와 사전에 예방할 수 있는 모니터링 및 예측이 중요하다. 본 연구는 고농도 미세먼지의 발생이 잦은 서울시의 PM_2.5를 local data assimilation and prediction system (LDAPS) 기상 관련 인자 15가지와 aerosol optical depth (AOD), 화학인자 4가지를 독립변수로 하여 앙상블 모델 두 가지 random forest (RF)와 extreme gradient boosting (XGB)로 예측하고자 하였다. 예측에 사용된 두 모델의 성능 평가와 인자 중요도 평가를 수행하였으며, 계절별 모델 분석도 수행하였다. 예측 정확도 결과, RF가 R² = 0.85, XGB가 R² = 0.91의 높은 예측 정확도를 보이며 XGB가 RF보다 PM_2.5 예측에 적합한 모델임을 확인하였다. 계절별 모델 분석 결과, 봄에 농도가 높은 관측 값과 비교하여 예측 수행이 잘 되었다고 할 수 있다. 본 연구는 다양한 인자를 이용하여 서울시의 PM_2.5를 예측하였고, 좋은 성능을 보이는 앙상블 기반의 PM_2.5 예측 모델을 구축하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제26권6호
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• pp.427-438
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• 2022

Pyroptosis, a form of cell death associated with inflammation, is known to be involved in diabetic nephropathy (DN), and discoid domain receptor 1 (DDR1), an inflammatory regulatory protein, is reported to be associated with diabetes. However, the mechanism underlying DDR1 regulation and pyroptosis in DN remains unknown. We aimed to investigate the effect of DDR1 on renal tubular epithelial cell pyroptosis and the mechanism underlying DN. In this study, we used high glucose (HG)-treated HK-2 cells and rats with a single intraperitoneal injection of streptozotocin as DN models. Subsequently, the expression of pyroptosis-related proteins (cleaved caspase-1, GSDMD-N, Interleukin-1β [IL-1β], and interleukin-18 [IL-18]), DDR1, phosphorylated NF-κB (p-NF-κB), and NLR family pyrin domain-containing 3 (NLRP3) inflammasomes were determined through Western blotting. IL-1β and IL-18 levels were determined using ELISA. The rate of pyroptosis was assessed by propidium iodide (PI) staining. The results revealed upregulated expression of pyroptosisrelated proteins and increased concentration of IL-1β and IL-18, accompanied by DDR1, p-NF-κB, and NLRP3 upregulation in DN rat kidney tissues and HG-treated HK-2 cells. Moreover, DDR1 knockdown in the background of HG treatment resulted in inhibited expression of pyroptosis-related proteins and attenuation of IL-1β and IL-18 production and PI-positive cell frequency via the NF-κB/NLRP3 pathway in HK-2 cells. However, NLRP3 overexpression reversed the effect of DDR1 knockdown on pyroptosis. In conclusion, we demonstrated that DDR1 may be associated with pyroptosis, and DDR1 knockdown inhibited HG-induced renal tubular epithelial cell pyroptosis. The NF-κB/NLRP3 pathway is probably involved in the underlying mechanism of these findings.

• 자원환경지질
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• 제55권3호
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• pp.281-293
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• 2022

연백, 연단, 밀타승으로 대표되는 납계열 안료의 대기환경에 따른 열화특성을 규명하였다. 자외선에 노출된 채색시편들은 모두 색차가 4~31로 어두워졌으나 성분에 변화는 없었다. CO₂는 시편의 색상 뿐 아니라 성분 변화에 큰 영향을 주지 못하였다. 반면 NO₂에 노출된 시편들은 색변화가 크게 나타나고 Lead nitrate(Pb(NO₃)₂)와 같은 2차 생성물이 확인되었다. 연백과 연단은 NO₂에 의한 색차가 각각 2와 10으로 시험 전에 비해 어두워졌으나 밀타승은 색차가 5이고 시험 전에 비해 밝게 변했다. 이를 통해 CO₂와 NO₂가 동일한 농도일 경우 CO₂에 비해 NO₂가 안료 보존에 영향력 있는 대기환경 인자임을 확인할 수 있다. 미세먼지에 존재하는 염의 영향을 보기 위해 5% NaCl(in distilled water)를 제조하여 염수 분무 시험을 실시하였다. 그 결과 연백, 연단, 밀타승 모두 시편 색변화와 함께 표면에 2차 생성물이 형성되었다. 이로 인해 아교도막이 박락되거나 hole이 형성되었으며, 생성물을 중심으로 색상이 변하였다. 또한 염수분무 후 XRD 피크 강도와 결정도가 함께 낮아진 것을 볼 수 있다. 염수분무 후 시료의 성분분석 결과 연백의 경우 Litharge가, 밀타승의 경우 Litharge와 Minium이 새로 형성되었다. 이를 통해 채색안료의 보존에 영향력 있는 대기환경 인자는 자외선, NO₂, 수용성 염임을 확인하였고 특히 납계열 안료 중 밀타승의 안정성이 다른 안료에 비해 낮은 것을 알 수 있었다.

• Geomechanics and Engineering
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• 제25권3호
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• pp.207-219
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• 2021

Several additives are used to enhance the geotechnical properties (e.g., shear wave velocity, shear modulus) of soils to provide sustainable, economical and eco-friendly solutions in geotechnical and geo-environmental engineering. In this study, piezoelectric ring actuators are used to measure the shear wave velocity of unreinforced, fiber, cemented, and fiber reinforced cemented Toyoura sand. One dimensional oedometer tests are performed on medium dense specimens of Toyoura sand-cement-fiber-silica flour mixtures with different percentages of silica flour (0-42%), fiber and cement (e.g., 0-3%) additives. The experimental results indicate that behavior of the mixtures is significantly affected by the concentration of silica flour, fiber and cement additives. Results show that with the addition of 1-3% of PVA fibers, the shear wave velocity increases by only 1-3%. However, the addition of 1-4% of cement increases the shear wave velocity by 8-35%. 10.5-21% increase of silica flour reduces the shear wave velocity by 2-5% but adding 28-42% silica flour significantly reduces the shear wave velocity by 12-31%. In addition, the combined effect of cement and fibers was also found and with only 2% cement and 1% fiber, the shear wave velocity increase was found to be approximately 24% and with only 3% cement and 3% fibers this increased to 35%. The results from this study for the normalized shear modulus and normalized mean effective stress agree well with previous findings on pure Toyoura sand, Toyoura silty sand, fiber reinforced, fiber reinforced cemented Toyoura sand. Any variations are likely due to the difference in stress history (i.e., isotropic versus anisotropic consolidation) and the measurement method. In addition, these small discrepancies could be attributed to several other factors. The potential factors include the difference in specimen sizes, test devices, methods of analysis for the measurement of arrival time, the use of an appropriate Ko to convert the vertical stresses into mean effective stress, and sample preparation techniques. Lastly, it was investigated that there is a robust inverse relationship between α factor and 𝞫₀ exponent. It was found that less compressible soils exhibit higher 𝜶 factors and lower 𝞫₀ exponents.

• 대한본초학회지
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• 제36권3호
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• pp.47-53
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• 2021

Objectives : Currently, the alopecia is one of the most emotionally stressful syndromes in human life. Human hair dermal papilla cells (HDPCs) play an essential role in controlling hair growth and in regulating hair cycle. We performed MTT assay, cell cycle, and western blot to determine the effects of essential oil from Coicis Semen (ECS) on hair growth in HDPCs. Methods : We monitored cell proliferations by MTT assay in HDPCs. After setting up the safe and effective concentration range to be treated ECS, cell cycle analysis was performed using flow cytometry. Also, the protein expression of hair growth-related factors such as insulin like growth factor-1 (IGF-1), Wnt, extracellular signal-regulated kinase (ERK), serine/threonine-specific protein kinase (Akt) in HDPCs was determined by western blot. Results : As results, cell proliferation was increased in ECS group compared to dimethyl sulfoxide (DMSO) group and minoxidil (MNXD) group. Cell number of ECS group was more decrease in sub G1 phase than cell number of DMSO group. Also, cell number of ECS group increased compared to cell number of DMSO group in G1 phase. Protein expression of ECS group was higher than protein expression of DMSO group on related hair growth factors (IGF-1, Wnt, ERK, Akt). Conclusion : As mentioned above, ECS increased cell proliferation and the protein expression of IGF-1, Wnt, ERK, and Akt. These results suggest that ECS could be used as a potential material for the treatment of alopecia by increasing the proliferation of HDPCs.

• Journal of Animal Science and Technology
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• 제64권5호
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• pp.985-996
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• 2022

This study investigated the effects of light intensity on growth performance, blood components, eye condition, and carcass characteristics of broiler chickens. Three hundred and fifty-two 1-day-old male chicks were assigned to one of four treatments (four repetitions per treatment, 22 birds per repetition) and reared in a floor pen for 5 weeks. From the second week, chicks were reared under four different levels of light intensity (5, 20, 35, and 50 lx) and the lighting duration was maintained at 18-hours light : 6-hours dark (18L : 6D). The feed intake and body weight were measured weekly. At 35 days of age, 12 birds per treatment were randomly selected for blood sampling, eye measurement, and carcass analysis. There were no significant differences in body weight gain, feed intake, and feed conversion ratio among treatments. Triglyceride levels in the serum were significantly higher in the 5 lx treatment, and creatinine was significantly lower in the 5 lx treatment (p < 0.05). The heterophil : lymphocyte ratios decreased significantly as light intensity increased (p < 0.05); however, other blood cell compositions were not affected by light intensity. Interleukin-6 content was significantly higher in the 5 lx treatment than in other treatments (p < 0.05), but the content of tumor necrosis factor-α was not significantly different among treatments. Serum corticosterone concentration was significantly higher at 5 lx than at 20, 35, and 50 lx (p < 0.05). The corneal diameter was the highest in 5 lx treatment (p < 0.05), and tended to increase as the light intensity decreased. Other eye parameters were not significantly different among treatments, but displayed a tendency to increase as the light intensity decreased. Carcass yield and part yields were not affected by light intensity. Meat quality parameters (pH, color, cooking loss, and water-holding capacity) did not show significant difference among the treatments. The results indicate that a light intensity of 5 lx may increase physiological stress or have a negative effect on broiler welfare, even if the performance and carcass characteristics are not affected. Therefore, a light intensity of 20 lx or above is recommended considering both the growth performance and welfare of broilers.

• 대한본초학회지
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• 제28권4호
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• pp.93-100
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• 2013

Objectives : This study was to evaluate the anti-inflammatory and anti-pruritic effects of WSY-1075 composited with Corni Fructus, Angelica gigantis Radix, Lycii Fructus, Ginseng Radix, Cervi parvum Cornu and Cinnamomi Cortex in rat peritoneal mast cells (RPMCs) and scratching mouse model. Methods : WSY-1075 was prepared by extracting with 30% ethanol. In the present study, we investigated the effect of WSY-1075 on the production of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$) and histamine in rat peritoneal mast cells (RPMCs) activated with phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187, and on the scratching behavior in mice treated with pruriogens. Results : WSY-1075 was not cytotoxic effect in used all concentration. PMA plus A23187 treatment significantly increased TNF-${\alpha}$, IL-$1{\beta}$ and IL-6 production compared with media control in RPMCs. However, TNF-${\alpha}$, $IL1{\beta}$ and IL-6 production increased by PMA plus A23187 treatment were significantly inhibited by WSY-1075 (200 ${\mu}g/mL$ and 400 ${\mu}g/mL$). WSY-1075 also inhibited the histamine release from RPMCs stimulated by compound 48/80, which promotes histamine release. Moreover, WSY-1075 administration had an inhibitory effects on the scratching behavior induced by pruritogen (compound 48/80, histamine, serotonin and substence P) in ICR mice. Conclusion : These results suggest that WSY-1075 administration (200 mg/kg or 400 mg/kg) has the anti-inflammatory and anti-pruritic effects on the activated rat peritoneal mast cell and mouse pruritus. WSY-1075 has a potential use as a composition of medicinal plants for treatment against inflammation- and pruritus-related disease.

• 한방재활의학과학회지
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• 제32권3호
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• pp.1-11
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• 2022

Objectives This study was designed to evaluate the healing effect of Cordyceps Militaris (CM) on collagen II-induced arthritis rats. Methods Sprague-Dawley rats were randomly divided into 6 groups (normal, control, positive control, CM with low/medium/high dosage each). Type II collagen mixed with complete Freund's adjuvant (with 1:1 v/v) was injected subcutaneously, and the mixture was injected in a same manner one week after the first injection to boost arthritis. Arthritis index, paw thickness and von Frey test were conducted to observe physical changes. hematoxylin and eosin (H&E) staining was performed to observe knee cartilage. The levels of messenger RNA (mRNA) expressions of interleukin (IL)-1𝛽, IL-6, tumor necrosis factor-alpha (TNF-𝛼) in spleen were assessed by real-time polymerase chain reaction. Results Rheumatoid arthritis is an autoimmune disease that occurs on multiple joints and can lead to temporary shape change of bones or organ failure in severe cases. Here, we aimed to determine the effect of CM extract on rheumatoid arthritis by measuring paw thickness, arthritis index, conducting von Frey test and H&E staining, and evaluating the level of IL-1𝛽, IL-6, TNF-𝛼. As a result, paw thickness, arthritis index significantly decreased in low concentration group, hind leg became less sensitive in all expermental groups. Also, histological analysis showed that the damage of knee cartilage was prevented in all experimental groups. The level of mRNA of IL-1𝛽, IL-6, and TNF-𝛼 in spleen was analyzed to decide the effectiveness of CM extract. IL-1𝛽 did not show significant change, but IL-6 and TNF-𝛼 showed significant decrease in at least one of the experimental groups. Conclusions CM showed protective effect on knee tissue destruction and improved the physical conditions of the leg involving arthritis. Also, it showed that CM has anti-inflammatory effect on specific cytokines inducing rheumatoid arthritis. In conclusion, this study demonstrated that the therapeutic potential of CM for the treatment rheumatoid arthritis, and set the foundation for the further studies.

• 생명과학회지
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• 제32권1호
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• pp.23-28
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• 2022

곤충에서 유래한 항균펩타이드 CopA3는 다양한 세포사멸 과정을 차단한다고 알려져 있다. 세균 톡신에 의한 상피세포 세포사멸이나 6-hydroxy dopamine이 야기하는 신경세포 세포사멸 모두를 차단한다. 연구자 등은 최근에 CopA3가 카스파제에 직접 결합하여 그들의 활성형-절단과정을 차단한다고 보고하였다. 하지만 강력한 CopA3의 항세포사멸 효능을 설명하기 위해서는 추가적인 규명이 필요한 실정이다. 본 연구에서는 세포사멸경로의 핵심억제인자인 survivin 발현에 미치는 CopA3의 영향을 확인하였다. 인간 대장상피세포(HT29)에 CopA3를 처리한 뒤 survivin 발현을 추적한 결과, survivin 단백질 양이 유의하게 증가함을 확인하였다. RT-PCR을 통해서 CopA3가 survivin 유전자의 전사를 증가시킴을 확인하였다. 그리고 CopA3 자극이 Sp1 발현을 증가시키는 사실과, Sp1 억제 물질인 tolfenamic acid 처리가 CopA3에 의한 survivin 증가를 차단한다는 결과들을 바탕으로 우리는 CopA3가 Sp1을 통해 survivin 발현을 유도한다는 최종 결론을 도출하였다. 한편 본 연구를 통해서 CopA3의 강력한 항세포사멸 효능을 설명할 수 있는 분자기작을 새롭게 제시하였다고 사려된다.

• 멤브레인
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• 제32권2호
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• pp.163-171
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• 2022

알코올의 탈수를 위하여 에너지 다소비 공정인 증류 공정을 투과증발 막 공정으로 대체하려는 연구가 많이 진행되어져 왔다. 대표적인 투과증발 막인 PDMS 분리막에 대한 시간의존적 분리 거동은 분리 메카니즘의 이해에 매우 중요하다. 따라서 본 연구에서는 50 wt% 에탄올-물 혼합용액에 대하여 50℃에서 막면적 1194 cm²인 PDMS/PSF 분리막 모듈의 시간의존적인 투과증발 분리 거동을 고찰하였다. 총 유속과 에탄올/물 분리계수는 투과증발 시간이 증가함에 따라서 초기에 증가하다가 다시 감소하였다. 초기 분리성능의 증가는 건조한 PDMS 분리막에 에탄올이 용해되는데 시간이 걸리기 때문이며, 후기 분리성능의 감소는 주입 탱크의 에탄올 농도가 시간에 따라서 감소하기 때문에 나타나는 현상이었다. 따라서 본 연구로부터 PDMS 분리막을 통한 에탄올의 투과는 용해-확산 메카니즘에 의해 발생된다는 것이 재확인되었다.