• Archives of Pharmacal Research
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• v.29 no.9
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• pp.752-756
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• 2006

Artemisia princeps Pampanini, which is called Ssajuarissuk in Korean (SS-1), was fermented with lactic acid bacteria (LAB) and their passive cutaneous anaphylaxis reaction-inhibitory activity was investigated. Of these fermented agents, SS-1 extract fermented with Bifidobacterium infantis K-525 (F-SS-1) most effectively inhibited the release of ${\beta}$-hexosamindase from RBL-2H3 cells induced IgE. In IgE-induced RBL-2H3 cells, F-SS-1 inhibited proinflammatory cytokines IL-6 and $TNF-{\alpha}$ mRNA expression. Oral administration of SS-1 and F-SS-1 to mice inhibited passive cutaneous anaphylaxis (PCA) reaction induced by IgE and scratching behaviors induced by compound 48/80. The inhibitory activity of F-SS-1 against scratching behaviors was more effective than that of SS-1. These findings suggest that the fermentation of SS-1 with LAB can increase its antiallergic activity.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.18 no.1
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• pp.135-153
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• 2005

Herbal mixture water extract of (Phellodendron amurense, Scuellaria baiklensis, Spphora flavescens, Lithospermum erythrorhizon, Mellaphis chinesis, Alumite, Zanthoxylum schinifolium, Glycyrrhiza uralensis), which exhibit several beneficial effects including acne and skin diseases, was tested for anti-microbial activity and anti-inflammation effects. The herbal mixture extract showed antimicrobial activity against Stapylococcus epidermis, Propionibacterium acne, and Malassezia furfur. The growth of Stapylococcus epidermis, Propionibacterium acne, and Malassezia furfur was inhibited allergy and LPS induced cyclooxygenase-2(COX-2)gene expression in RAW24.7macrophage. The results indicated th ear swelling and histamine release induced by compound 48/80 were dose-dependently reduced, ranging 11-38% and 11-56%, respectively. Furthermore the extract inhibited the expression of LPS-induced COX-2 proteins and mRNAs without an appreciable cytotoxic effects on RAW264.7 cells. The cytotoxicity of the extract using M7T assay showed the cytotoxicity of 7 and 18% against L929 cell line. Based on these results, it is concluded that the herbal mixture water extract can be applied to the acne and skin diseases therapy.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.2 s.33
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• pp.142-150
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• 2007

Objectives : This study was carried out to investigate the latest study tendency in mouse model of skin pruritus published since 2005 and to arrange various experimental methods. Methods : We examined the journal(such as Experimental dermatology and British journal of dermatology) and in Pubmed since 2005, regarded pruritus and scratching behavior model as key words. Results and Conclusion : 1. BALB/c, hairless, NC/Nga and ICR mice were the most used in scratching behavior model. According to scratching-induced agents. we need to select experimental mice. 2. There are various methods inducing skin pruritus; by cohabitation with NC/Nga mice having severe skin lesions, by injection intradermally(or subcutaneously) with agent inducing inflammation, by inducing contact dermatitis with TNCB, TNFB, destruction of skin barrier and by transgenic mice. 3. Injection intradermally(or subcutaneously) with agent inducing inflammation is the most used out of methods inducing skin pruritus. Compound 48/80, histamine, substance P and others(chloroquine, serotonin, carrageenin, TPA etc.) were included in agents inducing inflammation and pruritus in skin pruritus model. 4. Video camera, SCLABA system, MicroAct and acoustic evaluation system were included in evaluation methods of scratching behavior mouse model.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.5
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• pp.1188-1193
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• 2003

Sogunjung-tang(SGJT) has been used for the treatment of general weakness, digestive organ disease and so on. This study was carried out for the purpose of knowing the inhibitory effect on the Type I hypersensitivity and Inflammatory reactions of SGJT. The reasults were obtained as follows: SGJT(0.1, 0.5, 1, 2mg/g) concentration of dependently inhibited compound48/80 induced anaphylaxis reaction in mice. SGJT(2mg/g) also inhibited permeability of evans blue into peritoneal cavity in mice. SGJT reduced IgE, CRP, WBC and Platelets on egg albumin induced hypersensitivity. but serum NO was grown. According to above results, SGJT may be beneficial in the type I hypersensitivity and Inflammatory reactions by inhibition of histamine release from mast cells.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.5
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• pp.1270-1275
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• 2003

The purpose of this research was to investigate the effects of Kamihyungbangjihwang-tang (KHT) on immediate-type allergic reaction. KHT was composed of hyungbangjihwangtang (HJT) and Rubus coreanus Miquel (RC) and Aspalathus linear is (AL). KHT and HJT (500 mg/kg) inhibited the systemic anaphylaxis induced by compound 48/80 and inhibited the passive cutaneous anaphylaxis (PCA) induced by anti-dinitrophenyl (DNP)-IgE and DNP-human serum albumin (HSA) in vivo. In addition, KHT, HJT, RC and AL inhibited the release of histamine and increased the release of cAMP from rat peritoneal mast cells. The anti-allergic action of KHT was more potent than those of HJT. These results indicate that KHT may be useful for the prevention and treatment of type I allergy related disease via inhibition of histamine release from mast cells.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.5
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• pp.1293-1298
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• 2003

HGJT has been used for the treatment of general weakness, digestive organ disease and so on. This study was carried out for the purpose of knowing the inhibitory effect on the Type I hypersensitivity and Inflammatory reactions of Hwanggigunjungtang(HGJT). The reasults were obtained as follows: HGJT(0.1. 0.5. 1, 2mg/g) concentration of dependently inhibited compound48/80 induced anaphylaxis reaction in mice. HGJT(2mg/g) also inhibited permeability of evans blue into peritoneal cavity in mice. HGJT reduced IgE, CRP. WBC and Platelets on egg albumin induced hypersensitivity. But serum NO was grown. According to above results, HGJT may be beneficial in the type I hypersensitivity and Inflammatory reactions by inhibition of histamine release from mast cells.

• The Journal of Pediatrics of Korean Medicine
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• v.20 no.1
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• pp.69-83
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• 2006

We investigated the effect of Sungmagalkun-tang (SGT) on the allergy. We conformed compound 48/80-induced active systemic anaphylatic shock, anti-dinitrophenyl IgE-mediated passive cutaneous anaphylaxis and ovalbumin-induced anaphylatic shock. Also observed IL-4 and GM-CSF mRNA expression in ovalbumin-induced allergic lung tissue and RBL-2H3. Histamine release is measured in RBL-2H3. SGT inhibited active systemic anaphylatic shock, passive cutaneous anaphylaxis and ovalbumin-induced anaphylatic shock by oral administration. We observed that SGT was concentration-dependently reduced IL-4 and GM-CSF mRNA expression in ovalbumin-induced allergic lung tissue and RBL-2H3 by SGT. In addition, SGT reduced histamine release in RBL-2H3. These results indicate that SGT has anti-histamic effect and controls IL-4 and GM-CSF mRNA expression on allergy.

• Advances in Traditional Medicine
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• v.8 no.1
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• pp.24-31
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• 2008

In the present investigation, we studied the effect of alcoholic extract of Moringa oleifera (M. oleifera) seed kernels on various experimental models of bronchial asthma. Significant (P < 0. 05) increase in preconvulsion time was observed due to pretreatment with M. oleifera when the guinea pigs were exposed to either acetylcholine (Ach) or histamine aerosol. This bronchodilating effect of M. oleifera was comparable to ketotifen fumarate. Spasmolytic effect of M. oleifera was also observed by dose dependent inhibition of ideal contractions induced by Ach, 5HT, histamine and $BaCl_2$. Alcoholic extract of M. oleifera produced significant dose dependent protection by egg albumin and compound 48/80 induced mast cell degranulation. Pretreatment with alcoholic extract of M. oleifera also decreased carrageenan induced rat paw edema, which was comparable to that of standard diclofenac sodium. Minimum inhibitory concentration for alcoholic extract of M. oleifera was low as compared to cold-water extract and hot water extract when antimicrobial activity was tested against various respiratory pathogens like Escherichia coli (E. coli), Staphylococus aureus (S. aureus) and pseudomonas aeruginosa (P. aeruginosa). Our data suggest that antiasthmatic activity of M. oleifera seed kernels may be due to its bronchodilator, anti-inflammatory, mast cell stabilization and antimicrobial activity.

• The Korea Journal of Herbology
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• v.27 no.5
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• pp.37-44
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• 2012

Objectives : The present study was undertaken to determine whether the ethanol extact of Citri Pericarpium (CP, Pericarp of Citrus unshiu Markovich, Rutaceae) is effective against atopic dermatitis according to it's storage period. Methods : To evaluate antiallergic effect of CP and OCP (Old Citri Pericarpium) evaluated in vivo their inhibitory effects against passive cutaenous anaphylaxis (PCA) reaction induced by IgE-antigen complex and scratching behaviors by compound 48/80. The Anti-atopic effects were measured by contact dermatitis, prurient animal model and PCA reaction. Contact dermatitis in mice as a model of the Type IV reaction caused by Oxazolone. Results : The results showed that anti-pruritus effects, analgesic effects of CP was depends on its hesperetin contents. And It also showed that keep longer in storage appeared to be higher in hesperetin contents. Both CP and OCP(Old CP) have a dose-dependent analgesic action in acetic acid induced writhing syndrome. OCP Potently inhibited PCA reaction in mice, although OCP weakly inhibited in long term contact dermatitis model in mice. Conclusions : These results suggest that the Proportional to the storage period, Citri Pericarpium possesses analgesic effects and anti-allergic effects.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.15 no.1
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• pp.31-49
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• 2002

This Experimental study was done to research effects of Fagopyrum esculentum Moench(Me-mil) Extract on the anti-allergic effects. The results were obtained as follows : 1. All concentrations of Fagopyrum esculentum Moench Extract(Flours and seeds) inhibit histamine release under the vascular permeability response to intracutanenous injection. The result is proportion to concentration. But, rutin can't get considerable result. 2. All concentrations of Fagopyrum esculentum Moench Extract inhibit histamine release under the vascular permeability response to intraperitoneal injection of SD Rat in comparison with Diphenhydramine which is typical anti-histamine drug. 3. All concentrations of Fagopyrum esculentum Moench Extract inhibit histamine release under the vascular permeability response to per-oral during the three or five days. 4. In the result of quantification of histamine induced Compound $\frac{48}{80}$, Flours and seeds of Fagopyrum esculentum Moench Extract inhibit histamine release. 5. Among the fracination of Fagopyrum esculentum Moench Extract, $CHCl_3$ fraction inhibit histamine release effectively. 6. In the result of genetic manifestative inhibition about the Human mast cell treated PMA and A23187, Fagopyrum esculentum Moench Extract effect in the IL-4 and TNF-${\alpha}$ except IL-5. According to the above results, it is suggested that Fagopyrum esculentum Moench(Me-mil) Extract has anti-allergic effect.