• Journal of Ginseng Research
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• 제44권3호
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• pp.442-452
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• 2020

Backgroud: Sleep deprivation (SD) impairs learning and memory by inhibiting hippocampal functioning at molecular and cellular levels. Abnormal autophagy and apoptosis are closely associated with neurodegeneration in the central nervous system. This study is aimed to explore the alleviative effect and the underlying molecular mechanism of stem-leaf saponins of Panax notoginseng (SLSP) on the abnormal neuronal autophagy and apoptosis in hippocampus of mice with impaired learning and memory induced by SD. Methods: Mouse spatial learning and memory were assessed by Morris water maze test. Neuronal morphological changes were observed by Nissl staining. Autophagosome formation was examined by transmission electron microscopy, immunofluorescent staining, acridine orange staining, and transient transfection of the tf-LC3 plasmid. Apoptotic event was analyzed by flow cytometry after PI/annexin V staining. The expression or activation of autophagy and apoptosis-related proteins were detected by Western blotting assay. Results: SLSP was shown to improve the spatial learning and memory of mice after SD for 48 h, accomanied with restrained excessive autophage and apoptosis, whereas enhanced activation of phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway in hippocampal neurons. Meanwhile, it improved the aberrant autophagy and apoptosis induced by rapamycin and re-activated phosphoinositide 3-kinase/Akt/mammalian target of rapamycin signaling transduction in HT-22 cells, a hippocampal neuronal cell line. Conclusion: SLSP could alleviate cognitive impairment induced by SD, which was achieved probably through suppressing the abnormal autophagy and apoptosis of hippocampal neurons. The findings may contribute to the clinical application of SLSP in the prevention or therapy of neurological disorders associated with SD.

• 생명과학회지
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• 제25권1호
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• pp.53-61
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• 2015

본 연구에서 우리는 들깻잎(Perilla frutescens)으로부터 U937 세포에 대해 apoptosis를 유도하는 광과민성 물질을 분리하기 위해 연구를 시작하였다. 들깻잎은 한국의 대중적인 쌈채소로 바타민, GABA, 미네랄 등이 풍부하다고 알려져 있다. 건조한 들깻잎을 메탄올로 추출하여 다양한 크로마토그래피법을 이용하여 광과민성 물질을 순수 분리하였다. 순수 분리한 광과민성 물질을 PL9443으로 명명하고, 구조동정을 위하여 1D-NMR, 2D-NMR, FAB-mass spectroscopy 분석을 실시하였다. 순수 분리한 PL9443 compound의 흡광도를 측정한 결과 410 nm에서 최대 흡수를 보였고, 330, 410, 680 nm에서 흡수 peak를 나타내었다. PL9443 compound는 분자량 620.7의 pheophorbide a ethyl ester로 동정하였다. 구조동정한 물질은 porphyrin환을 갖는 구조에서 Mg이온이 탈리된 pheophorbide 유도체화합물이었다. PL9443 compound의 처리에 따른 U937 세포의 형태학적 변화를 관찰한 결과, 광조건에서 apoptosis의 전형적인 현상인 apoptotic body와 소낭구조물이 형성되는 것을 확인할 수 있었다. 또한 PL9443 compound를 0.08 nM로 처리하였을 때 99%의 caspase-3/7 activity가 나타났고, 세포손상 역시 광의존적으로 나타내는 것을 확인하였다. PL9443 compound를 처리하고 광을 조사한 처리구와 광을 조사하지 않은 처리구의 세포로부터 DNA를 분리하여 전기영동을 실시한 결과 광을 조사한 곳에서 DNA ladder현상을 관찰할 수 있었다. 따라서 PL9443 compound는 광의존적 apoptosis 유도에 의한 세포손상을 일으키는 것을 확인하였다.

• 대한본초학회지
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• 제22권2호
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• pp.25-33
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• 2007

Objectives : Oriental pear was used as treatment of asthma, control of blood pressure, diabetes in oriental medicine. The aim of this study was to observe the effects of Phenolic compound extracted from pear and herbal drugs to treat asthma. Methods : In order to study the effect of oral administration of phenolic compound extracted from pear and herbal drugs(Platycodon grandiflorum, Prunus armeniaca) on allergic asthma, mice were pre-treated by oral administration of the solution before antigen sensitization four times for 8 days. 2 days later, mice were actively sensitized with a subcutaneous injection of ovalbumin and 13 days later, they were provoked with ovalbumin aerosols. The animals were divided into four groups; Saline, orally administered saline. PC-A, orally administered Phenolic compound extracted from pear peel 10mg/kg/ml. PC-B, orally administered Phenolic compound extracted from pear peel and flesh 10mg/kg/ml. PC-C, orally administered pear 10m/kg/ml, Platycodon grandiflorum 24.4 mg/kg/ml and Prunus armeniaca 33.3 mg/kg/ml. Serum level of IgE, IL-4, cell numbers in the bronchoalveolar lavage fluid(BALF), and in vitro isometric contractile responses of the isolated tracheal smooth muscle(TSM) to acetylcholine(ACh, $0.1-1000{\mu}M$), KCl were measured. Results : Contractile responses of TSM to ACh were decreased in PC-A group at Ach 0.1, 0.3, 1 ${\mu}M$, decreased in PC-B at 0.1 ${\mu}M$ and decreased in PC-C at 0.1, 0.3, 1, 10, 30 ${\mu}M$. The maximal contractile response of TSM to KCl was decreased in PC-C group, The cell numbers of eosinophil in BALF were decreased in PC-C group, and those of macrophages in BALF were decreased in PC-A and PC-C group. Interleukin-4 in BALF was decreased in PC-A, PC-B, PC-C group. Conclusion : Based on the above results it is assumed that oral administration of phenolic compound extracted from pear and herbal drugs can help the treatment of deficiency allergic Asthma.

• 한국식품과학회지
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• 제39권1호
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• pp.77-82
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• 2007

쑥갓의 항산화 활성을 알아보기 위해 쑥갓 총 메탄올추출물의 아질산염 소거작용과 SOD 유사활성을 측정하였다. 아질산염 소거작용과 SOD 유사활성을 가지고 있는 총 메탄올 추출물로부터 클로로포름, n-부탄올 및 물 분획물을 제조하여 각각의 활성을 측정하였다. pH가 1.5일 때의 클로로포름 분획물의 $IC_{50}$값은 39ppm으로 가장 우수한 아질산염 소거작용을 보였으며, vitamin C와 chlorogenic acid의 $IC_{50}$값이 15ppm과 36ppm일 때 클로로포름 분획물은 양성대조군인 chlorogenic acid와 동등한 소거작용을 보였다. 아질산염 소거작용에 대하여 유의성 있게 높은 활성을 나타낸 클로로포름 분획물을 silica gel 컬럼크로마토그래피와 sephadex LH-20 컬럼크로마토그래피를 이용하여 분획의 활성성분인 compound I을 분리하였다. 분리된 compound를 $^{1}H-NMR$과 $^{13}C-NMR$ spectral data를 통하여 구조를 동정한 결과 compound I은 ${\beta}-sitosterol-O-{\beta}-D-glucoside$임을 확인하였다. Compound I과 그 aglycone인 ${\beta}-sitosterol$의 아질산염 소거작용과 SOD 유사활성은 다소 차이가 있었다. 양성대조군으로 사용한 vitamin C와 chlorogenic acid의 $IC_{50}$값이 pH 1.5에서 각각 15ppm과 36ppm일때 compound I의 $IC_{50}$값이 335ppm, ${\beta}-sitosterol$의 $IC_{50}$값이 41ppm으로 가수분해된 aglycone이 아질산염 소거작용이 훨씬 높았다. SOD 유사활성의 경우 vitamin C와 chlorogenic acid의 $EC_{50}$ 값은 38ppm, 449ppm으로 이와 비교시 ${\beta}-sitosterol$과 배당체의 $EC_{50}$ 값은 1,291ppm과 2,000ppm 이상으로 나타났다.

• 분석과학
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• 제24권2호
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• pp.135-141
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• 2011

세포외 기질의 주요 단백질 구성요소들을 가수분해시켜 재편성(turnover)과 재형성(remodeling)에 핵심 역할을 하는 효소가 matrix metalloproteinases (MMPs)이다. MMPs 중에서 MMP-2와 MMP-9는 catalytic domain이 fibronectin-like domain에 의해 hemopexin-like domain 부위와 떨어져 있는 점이 다른 MMP들과 다르다. MMP-9 억제제의 개발로 간암전이를 막을 수 있다고 보고되고 있다. Hovenia dulcis Thunberg에서 MMP-9의 활성을 저해하는 물질을 분리 정제하였고, 분리된 물질에 대한 MMP-9의 활성억제 여부를 확인하였다. Ethyl acetate (EA)에 의해 용출 분리된 두개의 화합물(화합물 A, 화합물 B)과 MeOH로 용출 분리된 한 개의 화합물(화합물 C)에서 MMP-9의 활성에 저해를 보였고, $^1H$와 $^{13}C$ NMR, GC-MS 그리고 IR로 이들의 구조를 분석하였다. 화합물 A는 hydroxyl기와 methoxyl기로 치환된 벤젠고리를 함유하는 화합물로 catechine 계열로 추정되었으며, 화합물 B와 C는 hydroxyl기와 methoxyl기로 치환된 벤젠 고리와 분자내 carbonyl기를 갖고 있는 nobiletin 계열의 화합물로 추정되었다. 그리고 화합물 A는 MMP-9 활성을 1.0%농도에서 76% 억제하였으며, 화합물 B는 동일한 농도에서 66% 억제하는 것으로 나타났다. 그리고 화합물 C는 1.0%농도에서 71% 억제하는 것으로 나타나 화합물 A가 MMP-9의 활성 저해능이 가장 좋은 것으로 나타났다.

• 한국운동역학회지
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• 제16권3호
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• pp.1-7
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• 2006

The Primary type of swinging motion in human movement is that which is characteristic of a pendulum. The two types of pendulums are identified as simple and compound. A simple pendulum consist of a small body suspended by a relatively long cord. Its total mass is contained within the bob. The cord is not considered to have mass. A compound pendulum, on the other hand, is any pendulum such as the human body swinging by hands from a horizontal bar. Therefore a compound pendulum depicts important motions that are harmonic, periodic, and oscillatory. In this paper one discusses and compares two algorithms of Newton's method(F = m a) and Euler's method (M = $I{\times}{\alpha}$) in compound pendulum. Through exercise model such as human body with weight(m = 50 kg), body length(L = 1.5m), and center of gravity ($L_c$ = 0.4119L) from proximal end swinging by hands from a horizontal bar, one finds kinematic variables(angle displacement / velocity / acceleration), and simulates kinematic variables by changing body lengths and body mass. BSP by Clauser et al.(1969) & Chandler et al.(1975) is used to find moment of inertia of the compound pendulum. The radius of gyration about center of gravity (CoG) is $k_c\;=\;K_c{\times}L$ (단, k= radius of gyration, K= radius of gyration /segment length), and then moment of inertia about center of gravity(CoG) becomes $I_c\;=\;m\;k_c^2$. Finally, moment of inertia about Z-axis by parallel theorem becomes $I_o\;=\;I_c\;+\;m\;k^2$. The two-order ordinary differential equations of models are solved by ND function of numeric analysis method in Mathematica5.1. The results are as follows; First, The complexity of Newton's method is much more complex than that of Euler's method Second, one could be find kinematic variables according to changing body lengths(L = 1.3 / 1.7 m) and periods are increased by body length increment(L = 1.3 / 1.5 / 1.7 m). Third, one could be find that periods are not changing by means of changing mass(m = 50 / 55 / 60 kg). Conclusively, one is intended to meditate the possibility of applying a compound pendulum to sports(balling, golf, gymnastics and so on) necessary swinging motions. Further improvements to the study could be to apply Euler's method to real motions and one would be able to develop the simulator.

• 청정기술
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• 제23권4호
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• pp.378-387
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• 2017

영구적 난연성기를 함유한 에폭시 수지를 얻기 위하여, 본 연구에서는 디하이드록시를 함유한 인 화합물[10-(2,5-dihydroxyphenyl)-9,10-dihydro-9-oxa-10-phospha phenanthrene-10-oxide, DOPO-HQ]과 디하이드록시를 함유한 실리콘 화합물(polydimethylsiloxane, hydroxyl terminated, PDMS)를 경화하지 않은 에폭시 프리폴리머(diglycidyl ether of bisphenol A, DGEBA)와 반응시킨 다음, 경화제(4,4-diaminodiphenylmethane, DDM)를 사용하여 경화반응 시킨 에폭시 수지를 제조하였다. 제조된 에폭시 수지의 각종 특성을 적외선 분광분석기(FTIR), 시차주사열량기(DSC), 열중량 분석기(TGA), 한계산소지수(LOI)/수직 연소 시험(UL 94-V test), 인장 및 충격 시험을 이용하여 분석하였다. 그리고 사용한 인 및 실리콘 화합물의 함량이 에폭시 경화물의 열적/기계적 성질 및 난연성에 미치는 영향을 조사하였다. 인 및 실리콘 성분이 함유된 에폭시 수지의 열적 및 기계적 물성이 단순 에폭시 수지의 물성에 비교하여 크게 향상되었다는 것을 알 수 있었다. 그리고 인 및 실리콘을 함유한 모든 에폭시 수지는 29.9 ~ 31.8%의 LOI 및 V-0 수준의 UL 94-V로 난연성 기준(LOI: 30% 이상, UL 94-V: V-0)을 통과한 반면, 단순 에폭시 수지는 LOI: 21.4% 및 UL 94-V: no rating으로 난연성 기준에 크게 못 미치는 것을 알 수 있었다.

• 한국의학물리학회지:의학물리
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• 제28권1호
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• pp.16-21
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• 2017

This study assessed the feasibility of a dual-channel (DC) compound method for film dosimetry. The red channel (RC) is usually used to ensure dosimetric quality using a conventional fraction dose because the RC is more accurate at low doses within 3 Gy than is the green channel (GC). However, the RC is prone to rapid degradation of sensitivity at high doses, while degradation of the GC is slow. In this study, the DC compound method combining the RC and GC was explored as a means of providing accurate film dosimetry for high doses. The DC compound method was evaluated at various dose distributions using EBT3 film inserted in a solid-water phantom. Measurements with $10{\times}20cm^2$ radiation field and $60^{\circ}$ dynamic-wedge were done. Dose distributions acquired using the RC and GC were analyzed with root-mean-squares-error (RMSE) and gamma analyses. The DC compound method was used based on the RC after correcting the GC for high doses in the gamma analysis. The RC and GC produced comparatively more accurate RMSE values for low and high doses, respectively. Gamma passing rates with an acceptance criterion of 3%/3 mm revealed that the RC provided rapid reduction in the high dose region, while the GC displayed a gradual decrease. In the whole dose range, the DC compound method had the highest agreement (93%) compared with single channel method using either the RC (80%) or GC (85%). The findings indicate that the use of DC compound method is more appropriate in dosimetric quality assurance for radiotherapy using high doses.

• 한국주조공학회지
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• 제32권1호
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• pp.50-56
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• 2012

Fusion bonding of cast iron and Al alloy is an effective way to improve the properties such as low inertia, high efficiency and corrosion resistance in machinery parts. In case of fusion bonding, intermetallic compound layers are formed at the interface between cast iron and Al alloy interface. It is important to control the intermetallic compound layers for improving bonding strength. The formation behavior of intermetallic compound layer by heat treatment has been investigated. Heat treatment was performed at temperature from $600^{\circ}C$ to $800^{\circ}C$ with $100^{\circ}C$ interval for an hour to investigate the phase transformation during heat treatment. Heat treated specimens were analyzed by using FE-SEM, EPMA and EDS. The EPMA/WDS results revealed that various phases were formed at the interface, which exhibited 4 distinct intermetallic compound layers such as ${\tau}_6-Al_{4.5}FeSi$, ${\tau}_2-Al_3FeSi$, ${\tau}_{11}-Al_5Fe_2Si $and ${\eta}-Al_5Fe_2$. Also, fine precipitation of ${\tau}_1-Al_2Fe_3Si_3$ phase was formed between ${\tau}_{11}$ and ${\eta}$ layer. The phase fraction in intermetallic compound layer was changed by heat treatment temperature. At $600^{\circ}C$, intermetallic compound layer of ${\tau}_6$ phase was mainly formed with increasing heat treatment time. With increasing heat treatment temperature to $800^{\circ}C$, however, ${\tau}_2$ phase was mainly distributed in intermetallic compound layer. ${\tau}_1$ phase was remarkably decreased with increasing heat treatment time and temperature.

• 농약과학회지
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• 제3권1호
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• pp.20-28
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• 1999

Chloronicotinyl 유도체의 기본 골격인 3-pyridylmethylamine의 amine기를 phosphite group으로 치환하여 chloronicotinyl 유도체를 합성하였고, 이들의 살충활성과 살균활성을 확인하였다. 살충활성 검정 결과, 500 ppm 에서 phosphonate에 methyl기와 butyl기가 치환된 compound 4와 2개의 butyl, 2,2,2-trifluorotehtyl, 2-ethylhexyl, phenyl기가 치환된 compound 5, 6, 7, 8이 벼멸구에 대해서 90%의 살충활성을 나타내었다. 그러나 이들 화합물들은 배추좀나방과 두점박이응애에 대해서는 낮은 살충활성을 나타내었으며, phosphonate에 halogen이나 heterocycle이 치환된 compound $10{\sim}21$에서도 벼멸구, 배추좀나방, 담배거세미나방, 두점박이응애, 복숭아혹진딧물에 대하여 거의 살충활성을 나타내지 않았다. Chloronicotinyl 유도체의 벼도열병균, 벼문고병균, 오이잿빛 곰팡이병균, 토마토역병균, 밀녹병균, 보리흰가루병균에 대한 살균활성을 검정한 결과, 10 ppm에서 phosphonate에 butyl기와 4-nitrophenyl기가 치환된 compound 10이 벼도열병균에 대해 86%의 살균활성을 보였다. 인 함유 chloronicotinyl 유도체들 중 일부가 높은 살균활성을 나타낸다는 것은 이 유도체들이 신규 살균제로 개발될 가능성이 있다는 것을 시사하고 있다.