• Microbiology and Biotechnology Letters
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• v.17 no.5
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• pp.481-488
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• 1989

A serum-free medium that could be used for the large-scale culture of mouse hybridoma to produce monoclonal antibodies was developed. The medium was based on a 1:1 mixture of Iscove's Modified Dulbecco's Medium and Ham's F-12, supplemented with insulin 10$\mu\textrm{g}$/$m\ell$, transferrin 10$\mu\textrm{g}$/$m\ell$, ethanolamine 10$\mu$M and selenium 30nM (designated EBM (enriched basal medium) with the supplements). The effect of various supplements of steroid hormones, vitamins, lipid and mineral salts was investigated and their optimal concentration was determined to replace fetal calf serum (PCS). These components were added respectively and then added by way of two or three combination to discern of which component combination was effective to the culture of hybridoma. As a result, serum-free medium KM3 (EBM with BSA 100$\mu\textrm{g}$/$m\ell$, mineral cocktail and 0.05% PEG) was deter-mined. The hybridoma Alps 25-3 cultured in this medium showed almost the same growth rate as in medium added with 2% fetal bovine serum. However, the antibody concentration from KM3 cultures was 80% of that obtained from culture with FCS. KM3 was also examined for the culture of other mouse hybridomas, KW, A4W & HCGK, and it was confirmed that it could support the growth of these hybridomas and the production of monoclonal antibodies.

• Journal of Astronomy and Space Sciences
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• v.28 no.2
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• pp.123-132
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• 2011

It is generally believed that the occurrence of a magnetic storm depends upon the solar wind conditions, particularly the southward interplanetary magnetic field (IMF) component. To understand the relationship between solar wind parameters and magnetic storms, variations in magnetic field polarity and solar wind parameters during magnetic storms are examined. A total of 156 storms during the period of 1997~2003 are used. According to the interplanetary driver, magnetic storms are divided into three types, which are coronal mass ejection (CME)-driven storms, co-rotating interaction region (CIR)-driven storms, and complicated type storms. Complicated types were not included in this study. For this purpose, the manner in which the direction change of IMF $B_y$ and $B_z$ components (in geocentric solar magnetospheric coordinate system coordinate) during the main phase is related with the development of the storm is examined. The time-integrated solar wind parameters are compared with the time-integrated disturbance storm time (Dst) index during the main phase of each magnetic storm. The time lag with the storm size is also investigated. Some results are worth noting: CME-driven storms, under steady conditions of $B_z$ < 0, represent more than half of the storms in number. That is, it is found that the average number of storms for negative sign of IMF $B_z$ (T1~T4) is high, at 56.4%, 53.0%, and 63.7% in each storm category, respectively. However, for the CIR-driven storms, the percentage of moderate storms is only 29.2%, while the number of intense storms is more than half (60.0%) under the $B_z$ < 0 condition. It is found that the correlation is highest between the time-integrated IMF $B_z$ and the time-integrated Dst index for the CME-driven storms. On the other hand, for the CIR-driven storms, a high correlation is found, with the correlation coefficient being 0.93, between time-integrated Dst index and time-integrated solar wind speed, while a low correlation, 0.51, is found between timeintegrated $B_z$ and time-integrated Dst index. The relationship between storm size and time lag in terms of hours from $B_z$ minimum to Dst minimum values is investigated. For the CME-driven storms, time lag of 26% of moderate storms is one hour, whereas time lag of 33% of moderate storms is two hours for the CIR-driven storms. The average values of solar wind parameters for the CME and CIR-driven storms are also examined. The average values of ${\mid}Dst_{min}{\mid}$ and ${\mid}B_{zmin}{\mid}$ for the CME-driven storms are higher than those of CIR-driven storms, while the average value of temperature is lower.

• Journal of the Korean Society of Food Culture
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• v.20 no.4
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• pp.451-458
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• 2005

For the storaging and frying stability in soybean oil, the kind of treating antioxidants were mixed tocopherol and phosphatidyl choline(PC), phosphatidyl ethanolamine(PE), phosphatidyl inpsitol(PI), phosphatidyl serine(PS), phosphatidic acid(PA), phosphatidyl glycerol(PG), treating amounts were 0.03%, 0.05%(w/w), respectively. Acid value(AV), peroxide value(POV) and oxidative stability index(OSI) were determined during storage period at $50^{\circ}C$ in soybean oil. Antioxidation effect at frying condition was determined through changes of smoke point. The antioxidation effect according to AV change was $PA>PC>PI{\geq}PG>PS{\geq}PE$, preventing effect about POV change was PA>PG>PC>PS>PE>PI and preventing effect about OSI decreasing was PI>PC>PA>PG>PS>PE. At the result, antioxidation effect of individual phospholipid components in soybean oil was appeared different result according to determination code. Determination result of preventing effect of SP decreasing according to frying at $180^{\circ}C$ during 20 hours was PA>PC>PG>PE>PI>PS. But treating effect of mixed tocopherol in preventing effect about AV, POV was not. In the case of storage at $50^{\circ}C$ in soybean oil, mixed tocopherol was affected as a kind of pro-oxidant. In OSI had some antioxidation effect, preventing effect of SP decreasing had not. Treatment of mixed tocopherol in soybean oil was undesirable as frying oil.

• Journal of the Korean Institute of Gas
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• v.22 no.2
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• pp.46-51
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• 2018

Hydrogen sulfide from landfill and sewage treatment plant is a major odor component and causes many civil petitions. Rapidly developing industries release hydrogen sulfide, an odorous gas, to the atmosphere. This study aims to develop a $10{\mu}mol/mol$ concentration level hydrogen sulfide primary standard gas for odor measurement. The hydrogen sulfide gas was prepared at a nominal concentration of $10{\mu}mol/mol$ in nitrogen using the gravimetric method described in ISO 6142. Replicate standard gases were produced in 4 aluminium cylinders, and their concentrations were verified by GC-AED. The uncertainty of production was less than 0.50 %, and the variation of the 4 replicates was 0.22 %. The wall adsorption of hydrogen sulfide in cylinders was 0.10 % at 1500 psi, and the concentration was estimated to be long-term stable for one year. The relative expanded uncertainty of the preparation consistency, adsorption and long-term stability of this hydrogen sulfide standard gas was less than 1.05 % (95 % of confidence level, k=2).

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.11 no.3
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• pp.290-294
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• 2018

Modern warfare exhibit a NCW (Network Centric Warfare) aspect through quick situation awareness and Command and Control. And Tactical messages operate on various tactical data links (TDLs). For monitoring TDL messages processed by data link processor(DLP), message analysers are generally developed as a component during system development. In addition, in order to forward TDL messages processed by DLP to the message anayzer, the interface messages between DLP and message analysers should be defined interface control document(ICD). We propose the methods using UDP or TCP packets provided by TDL layers that are delivered to DLP without communicating directly with DLP. Depending on TDL message standards and Interface Control Documents(ICDs), we design the message analyzer which communicates with data link processor using internal messages. Using known header field information from the TDL application standard such as JREAP-C, we can quickly filter candidate packets against collected packets and use the full data size information contained in the headers to verify the completed message. Because the methods proposed in this paper do not communicate directly with DLP, the methods proposed in this paper are enable to analyze the TDL application messages such as JREAP-C or Link-K without constraints in existing methods that should be reflected in the system configuration and ICD.

• Korean Journal of Food Science and Technology
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• v.39 no.3
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• pp.309-314
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• 2007

The physicochemical and sensory characteristics of rice wine, Yakju, made with different additions of red yeast rice (0, 10, 20, 30, and 40%) were investigated. The pH of the rice wine decreased and the total acidity increased with greater additions of red yeast rice. Furthermore, the total sugar content increased and the alcohol content decreased with increasing amounts of red yeast rice. The color L-value diminished and the color a- and b-values increased with increasing amounts of red yeast rice. Among the volatile flavor compounds, esters, aldehydes, alcohols, and acids were the most important aroma-active compounds identified in the rice wine. The sample with the 10% red yeast rice addition had the highest ester content, which is a primary component in fruit fragrance. The highest overall acceptability values in the sensory test for color, flavor, taste, and overall favorite were 5.10, 5.75, 5.15, 4.65, and 4.50, respectively, with the addition of 10% red yeast rice.

• Korean Journal of Food Science and Technology
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• v.50 no.5
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• pp.492-498
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• 2018

The current study investigated the effect of rice cultivars (Samkwang and Seolgaeng) and milling methods on various characteristics of rice bread. In dry milling, all component characteristics, except the water solubility index, were higher. In terms of the morphology of starch, Samkwang exhibited an angular shape, while Seolgaeng exhibited a round shape. Wet milled flour yielded fine polygonal starch compared to dry milled flour. In regard to the quality characteristics of bread, both wet milled Samkwang and dry milled Seolgaeng showed closely similar specific volumes of 2.90 mL/g and 2.86 mL/g, respectively. Hardness was in the range of 555.50 - 597.29 g for Seolgaeng and 776.78 - 823.06 g for Samkwang. Thus, although the flour characteristics were different, dry milled Seolgaeng, the rice flour of which is economical to produce and has baking characteristics similar to that of wheat flour, appears to be preferable.

• The Journal of the Korea Contents Association
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• v.8 no.3
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• pp.86-96
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• 2008

General development basis of letter system is recognized by formative value in terms of its function and structure. principle of clustered writing is the most significant feature of Hangeul typography as considered that it is based on function and formativeness. Thus, not only by changes with its form but also by its characteristic syllable combination, space structure is made as consonants and vowels are combined in single letter, then the combination develop into word, sentence, paragraph to make second, third space structure character. This character has significant impact on readability that is core function of typography. With this property, space structure character is regarded as very important component of Hangeul typography. First, space structure character of Hangeul typography is reviewed by relating it to visual perception of gestalt psychology and compared square-framed letter and framed latter By applying square-framed letter and framed latter in same sentence, legibility and readability were studied. Researcher has found that space structure character of Hangeul typography has significant impact on its function, and in terms of future design, it is very critical not only for design but also for communication environment as space structure formativeness of Hangeul typography interact with communication that is basic concept.

• Clinical and Experimental Pediatrics
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• v.51 no.3
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• pp.225-232
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• 2008

The mission of National Health Screening Program for Infant and Children is to promote and improve the health, education, and well-being of infants, children, families, and communities. Although the term 'diagnosis' usually relates to pathology, a similar diagnostic approach applies to the child seen primarily for health supervision. In the case of health, diagnosis determines the selection of appropriate health promoting and preventive interventions, whether medical, dental, nutritional, educational, or psychosocial. Components of the diagnostic process in health supervision include the health 'interview'; assessment of physiological, emotional, cognitive, and social development (including critical developmental milestones); physical examination; screening procedures; and evaluation of strengths and issues. Open and informed communication between the health professional and the family remains the most significant component of both health diagnosis and health promotion. Families complete medical history forms at their health supervision visit. Family-friendly questionnaires, checklists, and surveys that are appropriate for the child's age are additional tools to improve and update data gathering. This type of information helps initiate and inform discussions between the family and the health professional. This article provides a comprehensive review of current National Health Screening Program for Infant and Children in Korea.

• Earthquakes and Structures
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• v.19 no.3
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• pp.157-174
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• 2020

The in-situ pushover test differs from the shake-table test because it is performed outdoors and thus its size is not restricted by space, which allows us to test a full-size building. However, to build a new full-size building for the test is not economical, consequently scholars around the world usually make scale structures or full-scale component units to be tested in the laboratory. However, if in-situ pushover tests can be performed on full-size structures, then the seismic behaviors of buildings during earthquakes can be grasped. In view of this, this study conducts two in-situ pushover tests of reinforced concrete (RC) buildings. One is a masonry-infilled RC building with openings (the openings ratio of masonry infill wall is between 24% and 51%) and the other is an RC building without masonry infill. These two in-situ pushover tests adopt obsolescent RC buildings, which will be demolished, to conduct experiment and successfully obtain seismic capacity curves of the buildings. The test results are available for the development or verification of a seismic evaluation model. This paper uses ASCE 41-17 as the main evaluation model and is accompanied by a simplified pushover analysis, which can predict the seismic capacity curves of low-rise buildings in Taiwan. The predicted maximum base shear values for masonry-infilled RC buildings with openings and for RC buildings without masonry infill are, respectively, 69.69% and 87.33% of the test values. The predicted initial stiffness values are 41.04% and 100.49% of the test values, respectively. It can be seen that the ASCE 41-17 evaluation model is reasonable for the RC building without masonry infill walls. In contrast, the analysis result for the masonry infilled RC building with openings is more conservative than the test value because the ASCE 41-17 evaluation model is limited to masonry infill walls with an openings ratio not exceeding 40%. This study suggests using ASCE 41-17's unreinforced masonry wall evaluation model to simulate a masonry infill wall with an openings ratio greater than 40%. After correction, the predicted maximum base shear values of the masonry infilled RC building with openings is 82.60% of the test values and the predicted initial stiffness value is 67.13% of the test value. Therefore, the proposed method in this study can predict the seismic behavior of a masonry infilled RC frame with large openings.