• Title/Summary/Keyword: Complex plant

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Isolation and Optimization of Cultivating Conditions of Alkalophilic Strains for Biodegradation of Azo Dye (Azo 염료의 분해를 위한 호알카리성 균주의 분리 및 배양조건의 최적화)

  • Kim, Jeong-Mog;Chung, Hyun-Chae;Kwon, Oh-Jin
    • KSBB Journal
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    • v.14 no.6
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    • pp.718-723
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    • 1999
  • In order to treat of alkaline dye-processing wastewater, alkalophilic strains biodegrading azo dye, Acid red 1, is isolated from natural system, and optimal culture conditions are examined using response surface analysis, statistical analysis system program. 15 different species which grow in alkaline culture media are isolated from the effluent and river soil discharged from wastewater treatment plant in dye industrial complex. One strain which has the best decolorization efficiency is chosen, and named as AR-1. The result of the examination of carbon, nitrogen and phosphorus sources which have influence on growth and decolorization reveals that optimum carbon, nitrogen and phosphorus sources are 1.0% fructose, 1.0% polypeptone, 1.0% yeast extract and 0.5% $K_2HPO_4$, respectively. In order to optimize of biodegradation conditions of dye by response surface analysis, the characteristics of decolorization and cell growth according to culture temperature and time are monitered. The result shows that the one is optimum 34.77$^{\circ}C$ for 12.97 hours; the other at 34.73$^{\circ}C$ for 12.96 hours. While, optimal conditions of culture that satisfy both cell growth and decolorization are the temperatures from 32.86$^{\circ}C$ to 36.36$^{\circ}C$ and the period of 10.96 to 15.75 hours, respectively.

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Distribution Characteristics of Polycyclic Aromatic Hydrocarbons (PAHs) in Soils in Jeju City of Jeju Island, Korea (제주시 토양 중 다환방향족탄화수소류(PAHs)의 분포 특성)

  • Jin, Yu-Kyoung;Lee, Min-Gyu;Kam, Sang-Kyu
    • Journal of Environmental Science International
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    • v.15 no.5
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    • pp.405-415
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    • 2006
  • Sixteen soil samples around six areas (residental area, traffic area, power plant area, incineration area and factory area) where the stationary and mobile sources of polycyclic aromatic hydrocarbons (PAHs) are estimated to be emitted in Jeju City, were collected during Feburuary to March, 2004, and analyzed for 16 PAHs recommended by US EPA as primary pollutants to investigate their distribution characteristics. The concentrations of total PAHs (t-PAHs) and total carcinogenic PAHs $(t-PAH_{CARC})$ in soils of Jeju City were in the range of $21.7\sim264.2ng/g$ on a dry weight basis with a mean value of 87.2 ng/g and $6.3\sim118.0ng/g$ with a mean value of 33.4 ng/g, respectively. The concentrations of t-PAHs were low in comparison with those in soils of other domestic and foreign countries. The mean concentrations of t-PAHs and $(t-PAH_{CARC})$ with area decreased in the following sequences: traffic area> incineration area > factory area > power generation area > harbor area enli residental area. The correlation between t-PAHs and $(t-PAH_{CARC})$ were very high $(\gamma^2=0.9701)$, indicating that $(t-PAH_{CARC})$ concentration increases in proportion with t-PAHs. Comparing the distribution ratio of ring PAHs with area among 16 PAHs, it decreased in the order of 4-ring > 5-ring > 6-ring > 3-ring > 2-ring in all the areas except for harbor area. whitens for harbor area it was similar among 3-, 4- and 5-ring with high value. Low and no correlations between t-PAHs and soil compositions (organic matter content and particle size distribution) were observed, which is considered to be caused by the complex factors, such as the loading and characteristics of PAHs and diverse soil environment change, etc. From the examination of the three PAH origin indices, such as LMW/HMW (low molecular weight $2\sim3$ ring PAHs over high molecular weight $4\sim6$ ring PAHs), phenanthrene/anthracene ratio and fluoranthene/pyrene ratio, it can be concluded that the soil PAH contaminations were ascribed to strong pyrogenic origin in ail areas except for harbor area and to both pyrogenic and petrogenic origins.

Expression of the Floral Repressor miRNA156 is Positively Regulated by the AGAMOUS-like Proteins AGL15 and AGL18

  • Serivichyaswat, Phanu;Ryu, Hak-Seung;Kim, Wanhui;Kim, Soonkap;Chung, Kyung Sook;Kim, Jae Joon;Ahn, Ji Hoon
    • Molecules and Cells
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    • v.38 no.3
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    • pp.259-266
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    • 2015
  • The regulation of flowering time has crucial implications for plant fitness. MicroRNA156 (miR156) represses the floral transition in Arabidopsis thaliana, but the mechanisms regulating its transcription remain unclear. Here, we show that two AGAMOUS-like proteins, AGL15 and AGL18, act as positive regulators of the expression of MIR156. Small RNA northern blot analysis revealed a significant decrease in the levels of mature miR156 in agl15 agl18 double mutants, but not in the single mutants, suggesting that AGL15 and AGL18 co-regulate miR156 expression. Histochemical analysis further indicated that the double mutants showed a reduction in MIR156 promoter strength. The double mutants also showed reduced abundance of pri-miR156a and pri-miR156c, two of the primary transcripts from MIR156 genes. Electrophoretic mobility shift assays demonstrated that AGL15 directly associated with the CArG motifs in the MIR156a/c promoters. AGL18 did not show binding affinity to the CArG motifs, but pull-down and yeast two-hybrid assays showed that AGL18 forms a heterodimer with AGL15. GFP reporter assays and bimolecular fluorescence complementation (BiFC) showed that AGL15 and AGL18 co-localize in the nucleus and confirmed their in vivo interaction. Overexpression of miR156 did not affect the levels of AGL15 and AGL18 transcripts. Taking these data together, we present a model for the transcriptional regulation of MIR156. In this model, AGL15 and AGL18 may form a complex along with other proteins, and bind to the CArG motifs of the promoters of MIR156 to activate the MIR156 expression.

Effect of Environmental Factors on the Growth of Microcystis aeruginosa (Cyanobacteria) in Agricultural Reservoirs (농업용 저수지에서 환경 요인이 Microcystis aeruginosa (cyanobacteria) 성장에 미치는 영향)

  • Kwon, O-Chang;Park, Jung-Won;Chung, Gyu-Young;Lee, Jong-Eun;Seo, Eul-Won
    • Journal of Life Science
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    • v.21 no.8
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    • pp.1183-1189
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    • 2011
  • The present study is aimed at examining the effects of the physico-chemical environmental factors of water systems on water bloom at Homin and Gagok reservoirs in Pungcheon-Myeon, Andong, Gyeongsangbuk-do. The mean water temperature and the contents of chlorophyll-a, total-nitrogen, total-phosphorus and phosphate-phosphorus were higher at the Gagok reservoir. On the other hand, the pH mean value was higher at the Homin reservoir. The mean value of microelements (Na, K, Mg, Fe, Si) was higher at the Gagok reservoir. The cyanobacteria which was considered to be the cause of water bloom at the two reservoirs was Microcystis aeruginosa. It started to grow in May and showed the highest standing crop in August. Between the increase of standing crop of M. aeruginosa and the water quality, correlation values of $Na^+$ (r=-0.910, p<0.05), $Fe^{2+}$ (r=-0.855, p<0.05) and $Si^{4+}$ (r=0.989, p<0.01) at the Homin reservoir increased amount of standing crop. Meanwhile, at the Gagok reservoir, the contents of $Na^+$ (r=-0.776, p<0.05), $Si^{4+}$ (r=0.899, p<0.05) were highly related to the increase of standing crop. Interestingly, Si, which is the limiting factor for diatoms, has a high correlation with standing crop of cyanobacteria. In conclusion, the water blooming is caused not by a simple factor but a synergistic effect due to complex actions including high concentrations of nitrogen and phosphorus ions and many other environmental factors.

Dynamic Response Analysis of Pressurized Air Chamber Breakwater Mounted Wave-Power Generation System Utilizing Oscillating Water Column (진동수주형 파력발전 시스템을 탑재한 압축공기 주입식 방파제의 동적거동 해석)

  • Lee, Kwang-Ho;Kim, Do-Sam;Yook, Sung-Min;Jung, Yeong-Hoon;Jung, Ik-Han
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.26 no.4
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    • pp.225-243
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    • 2014
  • As the economic matters are involved, applying the WEC, which is used for controlling waves as well as utilizing the wave energy on existing breakwater, is preferred rather than installing exclusive WEC. This study examines the OWC mounted on a pressurized air chamber floating breakwater regarding the functionality of both breakwater and wave-power generation. In order to verify the performance as a WEC, the velocity of air flow from pressurized air chamber to WEC has to be evaluated properly. Therefore, numerical simulation was implemented based on BEM from linear velocity potential theory as well as Boyle's law with the state equation to analyze pressurized air flow. The validity of the obtained values can be determined by comparing the previous results from numerical analysis and empirically obtained values of different shapes. In the actual numerical analysis, properties of wave deformation around OWC system mounted on fixed type and floating type breakwaters, motions of the structure with air flow velocities are investigated. Since, the wind power generating system can be hybridized on the structure, it is expected to be applied on complex power generation system which generates both wind and wave power energy.

Comparison of Measured and Predicted $^3H$ Concentrations in Environmental Media around the Wolsung Site for the Validation of INDAC Code (주면피폭선량 평가코드(INDAC)의 검증을 위한 월성원전 주면 삼중수소 농도 실측치와 예측치의 비교 평가)

  • Jang, Si-Young;Kim, Chang-Kyu;Rho, Byung-Hwan
    • Journal of Radiation Protection and Research
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    • v.25 no.2
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    • pp.75-80
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    • 2000
  • The predicted results of INDAC code were compared with measured $^3H$ concentrations in air and pine-needle around the Wolsung site. The optimal sets of input data to INDAC were in addition selected by comparing the measured values with the predicted values of INDAC based on various conditions such as the release modes of effluents into the environment, the classification of wind classes, and the consideration of terrain. The predicted $^3H$ concentrations in air and pine-needle were shown to have good agreement with measured values, although there are some limitations such as uncertainties in measured values, complex topology around the site, and the land-sea breeze effects. The assumption on the $^3H$ behavior in vegetables or plants that the ratio of $^3H$ concentration in plant water to $^3H$ concentration in atmospheric water is 1/2 was shown to be conservative in terms of the audit calculation performed by the regulator. It was also found that data sets based on mixed mode and no terrain data were not appropriate for the audit calculation ensuring the compliance with regulations. Thus, if the mixed mode is considered as the release mode of effluents into the environment, meteorological data measured at 58 m height and terrain data should be used to evaluate the atmospheric dispersion factor.

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An Analysis on the Educational Needs for the Smart Farm: Focusing on SMEs in Jeon-nam Area (중소·중견기업의 스마트팜 교육 수요 분석: 전남지역을 중심으로)

  • Hwang, Doo-hee;Park, Geum-Ju
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.1
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    • pp.649-655
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    • 2020
  • This study determined effective educational strategies by investigating and analyzing the related educational demands for SMEs (small and medium-sized enterprises) in the 4th Industrial Revolution based area of smart farms. In order to derive the approprate educational strategies, Importance-Performance Analysis (IPA) and Borich's Needs Assessment Model were conducted based on the smart farm technological field. As a result, the education demand survey showed high demand for production systems and intelligent farm machinery. In detail, Borich's analysis showed the need for pest prevention and diagnosis technology (8.03), network and analysis SW linkage technology (7.83), and intelligent farm worker-agricultural power system-electric energy hybrid technology (7.43). In contrast, smart plant factories (4.09), lighting technology for growth control (4.46) and structure construction technology (4.62) showed low demands. Based on this, the IPA portfolio shows that the network and analysis SW linkage technology and the CAN-based complex center are urgently needed. However, the technology that has already been developed, such as smart factory platform development, growth control lighting technology and structure construction technology, was oversized. Based on these results, it is possible to strategically suggest the customized training programs for industrial sectors of SMEs that reflect the needs for efficiently operating smart farms. This study also provides effective ways to operate the relevant training programs.

A study on the basic design of bypass valve using CAE technology (CAE 기반 바이패스 밸브 기본설계에 대한 연구)

  • Oh, Jae-Won;Min, Cheon-Hong;Cho, Su-Gil;Park, Sang-Hyun;Kang, Kwan-Gu;Kim, Seong-Soon;Hong, Sup;Kim, Hyung-Woo
    • Journal of Advanced Marine Engineering and Technology
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    • v.40 no.7
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    • pp.663-670
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    • 2016
  • This paper introduces the concept of the computer-aided engineering(CAE) design method for a bypass valve in a system that is used for the safe lifting of mineral resources in deep-seabed mining. Although the bypass valve has a simple mechanism, its design is very difficult because of various influencing factors. This equipment, which has a complex design process, should be developed by CAE-based design method. The method can perform the design, design verification, and virtual experiment at the same time. In this study, the CAE-based method for the design of the bypass valve has been developed using fluid dynamics, multi-body dynamics, and optimization method.

Commercialization of Ion Exchange Fiber System for Recovering Valuable Metals in Plating Wastewater (도금 폐수 중 유가 금속 회수를 위한 이온교환섬유의 상용화기술)

  • You, Seong-sik
    • Korean Chemical Engineering Research
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    • v.55 no.4
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    • pp.535-541
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    • 2017
  • On the basis of 200 ppm of Ag and 120 l/h of feed flow rate, we built a pilot plant of an ion exchange fiber system having an double tube type ion exchange chamber with strong base ion exchange fiber (FIVAN A-6) which was designed to replace fibers easily and to eliminate the need for a fixture. The following results were obtained for the double tube type of ion exchange fiber system with an ion exchange capacity of 4.6 meq/g for Ag. The adsorption process was operated in the range of 40~90 l/h after confirming the effect of the flow rate and, pH did not affect formation of complex ion of Ag in the range of pH 7~12. In the case of backwash process, the recovery rate of Ag was tested in the range of 60~120 l/h and comparative experiments were carried out using NaOH, $NH_4Cl$, and NaCl as the chemicals for backwash. Although the desorption time was shortened at higher concentration, the desorption efficiency per mol was lowered. Therefore, it was confirmed that the desorption time and the concentration should be well balanced to operate economically. The desorption pattern of the backwash process is slower than the adsorption process and takes a lot of time. The results showed that the Ag adsorption ratio was 99.5% or more and the Ag recovery ratio was 96% or more, and commercialization was possible.

Development of an 1-Dimensional Dynamic Numerical Model for BTX Removal Process Analysis by Gaseous-Biofilm Filtration (기체상-생물막 여과 공법의 BTX 제거 공정 해석을 위한 1차원 동적 수치모델 개발)

  • Kim, Yeong-Kwan;Choi, Sung-Chan;Kim, Seog-Ku;Lee, Yong-Seok
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.12
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    • pp.689-695
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    • 2015
  • A biofilm filtration for the removal of gaseous pollutants has been recognized as a process with a complex interaction between the gas flow characteristics and the process operating variables. This study aims to develop an one dimensional dynamic numerical model which can be utilized as a tool for the analysis of biofilm filtration process operated in plug flow mode. Since, in a plug flow system, minor environmental changes in a gaseous unit process cause a drastic change in reaction and the interaction between the pollutants is an influencing factor, plug flow system was generalized in developing the model. For facilitation of the model development, dispersion was simplified based on the principles of material balance. Several reactions such as competition, escalation, and control between the pollutants were included in the model. The applicability of the developed model was evaluated by taking the calibration and verification steps on the experimental data performed for the removal of BTX at both low and high flow concentration. The model demonstrated a correlation coefficient ($R^2$) greater than 0.79 under all the experimental conditions except for the case of toluene at high flow condition, which suggested that this model could be used for the generalized gaseous biofilm plug flow filtration system. In addition, this model could be a useful tool in analyzing the design parameters and evaluating process efficiency of the experiments with substantial amount of complexity and diversity.