• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.58-58
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• 2003

The envelope glycoprotein of HIV, gp41, mediates the membrane fusion with human cells. The extracellular domain of gp41 has two helical regions. The N-terminus helical region (N-helix) forms trimeric coiled coil, interacts with the C-terminus helical region (C-helix) of gp41 to form a stable helical bundle structure. In this study, we have shown that the N-helix of gp41 has membrane interacting and disrupting abilities. It was localized into the interface of the lipidic phase and head group of the membrane. In contrast, the N-helix region with membrane fusion defective mutations could not bind to membrane. In addition, the N-helix bound on the membrane was released from the membrane by the C-helix, and the complex of the N- and C-helix did not interact with membrane. These results suggested that the membrane binding ability of the N-helix is necessary for the fusion activity of gp41, and such property is possibly controlled by the C-helm.

• 대한족부족관절학회지
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• 제25권2호
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• pp.80-88
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• 2021

Chronic Achilles tendon rupture is likely to result in functional impairment in gait and sports activity. The presence of a large defect secondary to retraction of the tendon ends, atrophy of the calf muscles, and vulnerable vascularity of the soft tissue envelope make it a challenging problem to treat. Surgical reconstruction aims to restore the length and tension of the gastrocnemius-soleus complex. Various surgical treatment options have been described, depending on several factors, including residual gap size after scar tissue removal, remaining tissue quality, and vascularity. Despite good results being reported, there is a lack of high-level, evidence-based clinical guidelines available to select the first-line surgical procedure. This paper overviews the current available surgical options for patients with chronic Achilles tendon rupture.

• 대한인간공학회지
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• 제34권4호
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• pp.313-326
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• 2015

Objective: The objective of this research is to quantitatively analyze muscle activities of arm and shoulder, according to direction in various types of one-handed manual material handling, based on surface electromyography. Background: Workers in industrial sites frequently carry out one-handed manual material handling using arm and shoulder muscles. Therefore, chronic load and accumulated fatigue occur to arm and shoulder muscles, which becomes a main cause of upper arm and shoulder musculoskeletal disorders. The shoulder muscles have widely range of motion, and complex interactions take place among various muscles including rotator cuff muscles. In this regard, research on interactions among should muscles, according to such various dynamic motions, is required. Method: Ten male subjects in their 20s participated in this research. This research considered upward, downward, leftward, rightward, forward and backward directions and fourteen muscles around arm and shoulder (biceps brachii and trapezius, etc.) as independent variables. The mean muscle activity was set as the dependent variable. This research extracted $4^{th}{\sim}7^{th}$ repetition signals according to ten times of repetitive muscle contraction, and analyzed the muscle activity concerned using the envelope detection technique. Results: The mean muscle activity of upward direction was analyzed highly statistically significant. The reason is that the effect of gravity works to arm and shoulder muscles. Also, it is conjectured that deformation of coracoacromial ligament was caused, and its contact pressure increased, due mainly to the shoulder flexion, and therefore load was analyzed high. Muscle activity was analyzed significantly low, according to concentric ballistic motion used in the concentric contraction phase by storing elastic energy in the eccentric contraction phase with a motion to bring the weight to the front of subject's body as to downward, leftward and backward directions. Because, elbow joint's flexion-extension motions mainly occurred, biceps brachii was analyzed high muscle activity as the prime mover. Conclusion: The information on the quantitative load of muscles can be applied to ergonomic work design for one-handed manual material handling to minimize muscle load. Application: This research has effectively identified muscle activity according to dynamic contraction by applying an envelope detection technique. The results can be used for ergonomic work design to minimize muscle load during the one-handed manual material handling, according to each direction. The research results are expected to be used for musculoskeletal disorder prevention and physiotherapy in the rehabilitation medical field, based on the muscle load of arm and shoulder in various directions.

• 한국산학기술학회논문지
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• 제17권1호
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• pp.159-164
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• 2016

본 논문은 전자식 조정장치 헬리콥터의 비행영역 보호를 위한 하중제안 로직 설계에 대한 결과이다. 헬리콥터는 로터, 동체, 엔진등 구조가 복잡하기 때문에 여러 가지 제약조건에 가지고 비행을 해야 한다. 이 때문에 조종사가 그 제약조건을 고려하여 조종을 하면서 조종사의 작업부하가 증가하고 비행 조종성을 떨어뜨리는 결과가 발생한다. 이러한 헬리콥터의 비행제약조건으로부터 자유롭게 조정 하도록 하여 조종사를 도와주는 기능이 필요하고 본 논문에서는 그 중의 대표적인 제약조건인 하중제약조건에 대한 제한 로직을 전자식 조정장치 헬리콥터에 설계하고자 한다. 하중범위를 벗어나는 비행은 피치 싸이클릭 스틱을 급격하게 입력하여 주로 발생한다. 이때 조종스틱 명령과 피치축 자세명령모델사이에 비행영역제한로직을 추가하여 하중범위를 벗어나지 않도록 하였다. 현재의 하중 값에 대한 자세범위를 동적으로 계산하는 로직을 사용하였고 헬리콥터 모델에 적용하여 시뮬레이션을 통하여 알고리즘을 검증하였다. 시뮬레이션을 통하여 정지비행영역, 전진비행영역에서 하중제한로직을 적용하지 않았을 때와 적용하였을 때를 비교한 결과, 하중제한로직을 적용하지 않았을 때는 하중제한 리미트를 20% 이상 초과하였으나 본 연구에서 제한한 로직을 적용하였을 때는 하중제한 리미트가 초과하지 않음을 확인하였다. 결론적으로 시뮬레이션을 통하여 동적으로 할당하는 제한로직이 헬리콥터 전자식 조정장치 제어기에 적합함을 확인하였다.

• Nuclear Engineering and Technology
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• 제52권7호
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• pp.1571-1578
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• 2020

As a critical material in very/high-temperature gas-cooled reactors, graphite material directly affects the safety of the reactor core structures. Owing to the complex structures of graphite material in reactors, the material typically undergoes complex stress states. It is, therefore, necessary to study its mechanical properties, failure modes, and strength criteria under complex stress states so as to provide guidance for the core structure design. In this study, compressive failure tests were performed for graphite material under the condition of different confining pressures, and the effects of confining pressure on the triaxial compressive strength and Young's modulus of graphite material were studied. More specifically, graphite material based on the fracture surfaces and fracture angles, the graphite specimens were found to exhibit four types of failure modes, i.e., tension failure, shear-tension failure, tension-shear failure and shear failure, with increasing confining pressure. In addition, the Mohr strength envelope of the graphite material was obtained, and different strength criteria were compared. It showed that the parabolic Mohr-Coulomb criterion is more suitable for the strength evaluation for the graphite material.

• 한국전자파학회논문지
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• 제19권12호
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• pp.1339-1349
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• 2008

본 논문에서는 입력 복소 포락선 신호와 m차 고조파 대역의 출력 복소 포락선 신호 사이의 비선형성 관계를 이론적으로 분석하고, 이를 모델링하기 위하여 AM/$AM_m$, AM/$PM_m$을 정의하였다. 제안된 모델을 측정 데이터로부터 추출하는 방안을 제안하였고, InGaP 전력 증폭기에 대하여 기본 주파수 대역과 3차 고조파 대역에서의 신호를 측정하여 제안된 모델 및 기법의 유효성을 검증하였다 또한, 제안된 고조파 신호 대역에 대한 모델을 기반으로 고조파 대역 신호의 선형화를 위한 디지털 사전 왜곡 기법을 제안하였다 디지털 사전 왜곡기에서 비선형역함수 구현을 위해 수치 해석적인 방법과 Look-Up Table(LUT) 방식을 이용하였다. 고조파 신호 선형화를 위한 디지털 사전 왜곡기를 포함하는 송신기를 구현하고 16-QAM과 64-QAM의 입력 신호에 대해서 3차 고조파 출력신호의 스펙트럼과 I/Q 신호 성상도를 측정하였다. 고조파 대역에서 비선형 왜곡에 의해서 의미 없는 신호는 제안된 기법으로 구성된 송신기에 의해 선형화되었으며, 그 결과 각 신호에 대한 Error-Vector Magnitudes(EVM)이 6.4 %와 6.5 %로 측정되었다. 제안된 기법은 향후 SDR과 CR 등의 차세대 다중 대역 송신 시스템에 적용될 수 있을 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제27권6호
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• pp.1098-1105
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• 2017

Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral, and artificial viral vectors. The objective of this study was to establish a potential approach for large-scale production of VSV-G. To this end, VSV-G was cloned with an N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones ($Mut^s$) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, whereas the proliferation was reduced significantly under induction conditions. VSV-G protein was detected in cell lysates by western blot analysis, and the highest expression level was observed at 96 h post induction. VSV-G could also be obtained from the condition medium of yeast protoplasts. Furthermore, VSV-G could be incorporated into Ad293 cells and was able to induce cell fusion, leading to the transfer of cytoplasmic protein. Finally, VSV-G-mediated DNA transfection was assayed by flow cytometry and luciferase measurement. Incubation of VSV-G lysate with the pGL3-control DNA complex increased the luciferase activity in Ad293 and HeLa cells by about 3-fold. Likewise, incubation of VSV-G lysate with the pCMV-DsRed DNA complex improved the transfection efficiency into Ad293 by 10% and into HeLa cells by about 1-fold. In conclusion, these results demonstrate that VSV-G could be produced from P. pastoris with biofunctionalities, demonstrating that large-scale production of the viral glycoprotein is feasible.

• Journal of Microbiology and Biotechnology
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• 제27권12호
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• pp.2199-2210
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• 2017

Soil burial is the most widely used disposal method for infected pig carcasses, but composting has gained attention as an alternative disposal method because pig carcasses can be decomposed rapidly and safely by composting. To understand the pig carcass decomposition process in soil burial and by composting, pilot-scale test systems that simulated soil burial and composting were designed and constructed in the field. The envelope material samples were collected using special sampling devices without disturbance, and bacterial community dynamics were analyzed by high-throughput pyrosequencing for 340 days. Based on the odor gas intensity profiles, it was estimated that the active and advanced decay stages were reached earlier by composting than by soil burial. The dominant bacterial communities in the soil were aerobic and/or facultatively anaerobic gram-negative bacteria such as Pseudomonas, Gelidibacter, Mucilaginibacter, and Brevundimonas. However, the dominant bacteria in the composting system were anaerobic, thermophilic, endospore-forming, and/or halophilic gram-positive bacteria such as Pelotomaculum, Lentibacillus, Clostridium, and Caldicoprobacter. Different dominant bacteria played important roles in the decomposition of pig carcasses in the soil and compost. This study provides useful comparative date for the degradation of pig carcasses in the soil burial and composting systems.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2005년도 제20차 학술대회 논문집
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• pp.52-52
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• 2005

Oogenesis, the gonadosomatic index (GSI), reproductive cycle and first sexual maturation of the female Neptunea (Barbitonia) arthritica cumingii have been investigated by light and electron microscope observations. In the early vitellogenic oocyte, the Golgi complex and mitochondria were involved in the formation of glycogen, lipid droplets and yolk granules. In late vitellogenic oocytes, the rough endoplasmic reticulum and multivesicular bodies were involved in the formation of proteid yolk granules in the cytoplasm. In particular, compared with the results of other gastropods, it is a different result that appearances of cortical granules at the cortical layer and microvilli on the vitelline envelope, which is associated with heterosynthetic vitellogenesis, were not observed in vitellogenic oocytes during oogenesis. A mature yolk granule was composed of three components: main body (central core), superficial layer, and the limiting menbrane, Monthly changes in the gonadosomatic index in females were studied in 2002 and 2003 were closely associated with ovarian developmental phases. Spawning occurred between May and August in 2002 and 2003 and the main spawning occurred between June and July when the seawater temperature rose to approximately 18${\sim}$23${\circ}$C. The female reproductive cycle can be classified into five successive stages: early activestage (Septmber to October), late active stage ( November to February), ripe stage (February to June), partially spawned stage (May to Aygust), and recovery stage (June to August).

• 한국디지털건축인테리어학회논문집
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• 제13권4호
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• pp.25-32
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• 2013

The green building is one of biggest factors to go the goal of energy saving and environmental conservation, reduction of energy consumption, friendly energy technology, recycling of resource, and environmental pollution reduction technology. The purpose of these green buildings realized by the energy-saving technology such as the exterior materials or curtain wall system. The curtain wall system is a element that come to insulated portions of building envelope that results in heat loss. The purpose of this paper is to carried out mock-up tests for exterior wall used in autoclaved lightweight concrete panels in green building practices. Mock-up test execute a mixed process between standard test procedure and complex test procedure based on AAMA 501(American Society for Testing and Materials) and ASTM 283, ASTM 330(American Society for Testing and Materials). In results, tests meet the requirements that grant values in steps of procedures provided on ASTM and AAMA. ALC panel is suitable for a exterior wall product to be gratified thermal cycling performance and structural capacity, deflection(H/200) and lateral displacement(H/50), for curtain walls.