• 약학회지
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• 제52권6호
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• pp.426-433
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• 2008

The interaction of cadmium (II) ion with protocatechuic acid ($H_3PA$) and ethylenediaminetetraacetic acid (EDTA) were investigated in aqueous solution at different pH. The protocatechuic acid and EDTA/cadmium stochiometries for cadmium (II) binding have been determined by UV-vis spectrophotometric method. The complexation of Cd (II) ion with protocatechuic acid was formed in solution. Among the two potential sites of chelation present in the protocatechuic acid structure, the carboxylic function presents higher complexation power toward Cd (II). 1 : 1 Cd (II)-complex had a maximum absorbance and showed the bathochromic shift of the long-wavelength band of the UV-vis spectra in the alkaline pH when interacted with protocatechuic acid in 0.2 M $NH_3$ - 0.2 M ${NH_4}Cl$ (pH 8.0) buffer. These results suggest that Cd $({H_2}PA)^+$ complex has the optimal condition of chelation in buffer solution at 64.22 ${\mu}M$ protocatechuic acid (A=1.01455).

• Macromolecular Research
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• 제14권3호
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• pp.267-271
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• 2006

Polyelectrolyte complex films were prepared with two compounds, chitosan and poly(ethylene glycol)-monosuccinate, using a casting in order to synthesize a polyelectrolyte complex film with various mole ratios of chitosan and poly(ethylene glycol)-monosuccinate. The solution properties of isolated PEC were investigated for the effects of FTIR, pH value, Brookfield viscosity and cell viability assay using MTT staining. The PEC films were evaluated for mechanical properties by typical stress-strain curve, far thermal properties by DSC and TGA and for surface morphology Properties by SEM. Furthermore, the surface resistance, moisture regain and water content of the films were characterized. The solution properties were affected by several factors including the chitosan content in the PEC, the mixing ratio of PEG and chitosan, and pH. Several PEC in acidic conditions exhibited film formation under appropriate conditions of mixing ratio and chitosan concentration in the mixing process. These PEC films were found to have sufficiently flexible and stable properties due to their hydrophilic structure, which was farmed by the oppositely charged interaction between PEG-MS and chitosan matrix. The results showed the potential applicability of chitosan and poly(ethylene glycol)-monosuccinate films as a biocompatible polymer.

• Biomolecules & Therapeutics
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• 제29권5호
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• pp.527-535
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• 2021

Sclerostin (SOST), a regulator of bone formation in osteocytes, inhibits the canonical Wnt signaling by interacting with low-density lipoprotein receptor-related protein 5/6 (LRP5/6) to prevent Wnt binding. Loss-of-function mutations of the SOST gene caused massive bone outgrowth and SOST-null mouse exhibited a high bone density phenotype. Therefore, SOST has been suggested as a promising therapeutic target for osteoporosis. A few previous studies with X-ray crystallography identified the binding interfaces between LRP6 and SOST, but there are limitations in these studies as they used truncated SOST protein or SOST peptide. Here, we analyzed the conformational dynamics of SOST-LRP6 E1E2 complex using hydrogen/deuterium exchange mass spectrometry (HDX-MS). We examined the effect of the C-terminal tail of SOST on LRP6 conformation upon complex formation. HDX-MS analysis suggested a new potential binding interface for the C-terminal region of SOST that was missing from the previous crystal structure of the SOST-LRP6 E1E2 complex.

• 한국실내디자인학회:학술대회논문집
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• 한국실내디자인학회 2008년도 춘계학술발표대회 논문집
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• pp.116-122
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• 2008

As a model house has attempted a role change from a conceptual perspective as a housing cultural center starting from the 90ties, it began to direct the change into a high end building. In particular, large construction companies have recently invested on an unimagined scale, unlike in the past, in building a model house and pursued the construction of new attractions of cities as well as the upgrade of a brand image by hiring and implementing the designs of celebrity architects or designers. In addition, they have been in pursuit of a change by enhancing the satisfaction and pride of residents by offering spaces and programs for various cultural arts for apartment residents or potential customers and having them participate in those programs. This study aims to expound on the possibility of the utilization of a housing cultural center as a local complex community center by analyzing the spatial organization and the operational program of a housing cultural center appearing since the 90ties. With this aim, the study analyzed the cases of a complex community center and the program and the operational method of a housing cultural center within the case study in order to propose the possibilities for the utilization of a local complex community center. The elevation of the cultural and economic level of citizens and a rapid social change are shown as various demands for welfare, and the subsequent social physical change has led to the expansion of a space as a complex community. A housing cultural center is also directed to grow as a complex community center from the perspective of diverse demands and participations of consumers, and prepares a field for a broad spectrum of communications from the perspective of housing and cities.

• 동의생리병리학회지
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• 제35권5호
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• pp.193-198
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• 2021

The purpose of this study is to observe the effect of Cordyceps Militaris(CM) complex extract treatment on patients diagnosed with rheumatoid arthritis(RA). We reviewed medical records of 9 rheumatoid arthritis patients who visited the pain & rehabilitation center, Daejeon Korean Medicine Hospital of Daejeon University and were treated with CM complex extract from October 14, 2019 to October 14, 2020. We analyzed the records of DAS 28(Disease Activity Score 28), CDAI(Clinical Disease Activity Index), SDAI(Simplified Disease Activity Index), VAS(Visual analogue Scale), and KHAQ-D(Korean Health Assesment Questionnaire-Disability Index) before and after treatment to evaluate the effects of CM complex extract on RA. As a result, the scores of DAS 28(p<0.05), CDAI(p<0.01), SDAI(p<0.01) and VAS(p<0.01) were statistically significantly decreased, after 8 weeks of taking CM complex extract in RA patients. In conclusion, CM complex extract treatment may have potential effects of reducing disease activity and pain and improving quality of life in patients diagnosed with RA.

• 한국자기공명학회논문지
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• 제19권2호
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• pp.67-73
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• 2015

The microcystin is a cyclic heptapeptide from metabolites of cyanobacteria in the genera mycrocystis, anabaeba as a result of eutrophication. It has been known that microcystin-LR is a potent inhibitor of the catalytic subunits of protein phosphatase-1 (PP-1) as well as powerful tumor promoter. The active site of microcystin actually has two metal ions $Fe^{2+}/Zn^{2+}$ close to the nucleophilic portion of PP-1-microcystin complex. We report the isolation and purification of this microcystin-LR from cyanobacteria (blue-green algae) obtained from Daechung Dam in Chung-cheong Do, Korea. Microcystin-LR was extracted from solid-phase extraction (SPE) sample preparation using a CN cartridge. The cyanobacteria extract was purified to obtain microcystin-LR by HPLC method and identified by LC/MS. The detail structural studies that can elucidate the possible role of monovalent and divalent metal ions in PP-1-microcystin complexation were carried out by utilizing molecular dynamics. Conformational changes in metal binding for ligands were monitored by molecular dynamic computation and potential of mean force (PMF) using the method of the free energy perturbation. The microcystin-metal binding PMF simulation results exhibit that microcystin can have very stable binding free energy of -10.95 kcal/mol by adopting the $Mg^{2+}$ ion at broad geometrical distribution of $0.5{\sim}4.5{\AA}$, and show that the $K^+$ ion can form a stable metal complex rather than other monovalent alkali metal ions.

• 복식문화연구
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• 제15권2호
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• pp.193-202
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• 2007

The purpose of this study was to find out effects of textile motifs and the application methods on wearer's image perception. A survey was conducted to total of 255 male and female university students who are residing in Daejeon and Chungnam province. The stimuli were composed of 2 level tones(dark and light), 3 level complexity(simple, medial and complex), 3 patterns(polka dot, stripe and check) and the 2 way of stimuli application methods(fabric and garment). The instrument for measuring preference of stimuli consisted of 4 items, encouraging, preference, purchasing and popularity. The instrument for measuring image of stimuli consisted 24 pair items. Factor analysis for the adjective pair images(24 inquiries) about the textile patterns which were used in this study was performed. It resulted as three factors which are attraction, salience, and potential. Attraction, salience, and potency dimensions showed the most significant interaction effects of application methods and patterns. And tone and application method effected attraction and salience, tones and patterns effected attraction, tones and complex effected salience. Application methods and patterns effected potential and patterns and complex effected salience. The preferences toward stimuli, it resulted only interaction of tones and patterns affected the preferences('total preference' and 'purchasing'). Pearson's product-moment correlation analysis carried out to find out the relation of images of clothing and preferences. As a result, salience was significant relation with attraction and potency. In correlation between image of textile pattern and preference, attraction is most significant relation with the preference. The results of the study could be used for the marketing strategies of the motif in fashion product.

• 대한화학회지
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• 제41권11호
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• pp.590-593
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• 1997

Germanium(IV)-Morin 착물에 대한 네모파 양극벗김 전압전류법적 연구를 0.5 M 황산 지지전해질에서 수행하였다. Germanium(IV)-Morin 착물의 피크전위는 - 0.606 V vs. Ag/AgCl에서 나타났다. Germanium(IV)-Morin 착물의 피크전류 크기에 미치는 지지전해질의 황산 농도, Morin의 농도, 축적전위 및 축적시간의 의존성 등에 대하여 논의하였다. 또한, Germanium(IV)-Morin 착물의 피크전류 크기에 영향을 주는 금속 양이온들의 방해효과도 조사하였다. 이 방법을 적용시켜 축적시간을 60초로 하였을 때 Germanium(IV)의 검출한계는 $3.76{\times}10^{-7}M(27 {\mu}g/L)$ 이었으며, 0.4 mg/L($5.5{\times}10^{-6}$ M)의 Germanium(IV) 분석에 대한 상대표준편차(n=8)는 3.2% 이었다.

• Wind and Structures
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• 제20권1호
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• pp.15-36
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• 2015

Characterization of wind flows over a complex terrain, especially mountain-gorge terrain (referred to as the very complex terrain with rolling mountains and deep narrow gorges), is an important issue for design and operation of long-span bridges constructed in this area. In both wind tunnel testing and numerical simulation, a transition section is often used to connect the wind tunnel floor or computational domain bottom and the boundary top of the terrain model in order to generate a smooth flow transition over the edge of the terrain model. Although the transition section plays an important role in simulation of wind field over complex terrain, an appropriate shape needs investigation. In this study, two principles for selecting an appropriate shape of boundary transition section were proposed, and a theoretical curve serving for the mountain-gorge terrain model was derived based on potential flow theory around a circular cylinder. Then a two-dimensional (2-D) simulation was used to compare the flow transition performance between the proposed curved transition section and the traditional ramp transition section in a wind tunnel. Furthermore, the wind velocity field induced by the curved transition section with an equivalent slope of $30^{\circ}$ was investigated in detail, and a parameter called the 'velocity stability factor' was defined; an analytical model for predicting the velocity stability factor was also proposed. The results show that the proposed curved transition section has a better flow transition performance compared with the traditional ramp transition section. The proposed analytical model can also adequately predict the velocity stability factor of the wind field.

• 멤브레인
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• 제13권1호
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• pp.37-46
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• 2003

하전된 멤브레인 미세기공으로 유체가 흐르는 경우는 계면동전기 효과가 작용하게 된다. 비선형 Poisson-Boltzmann 전기장과 흐름에 의해 유발되는 전기장 사이의 정전상호작용을 운동방정식의 외부작용 힘으로 고려하였다. 유한차분법으로 정전위 분포를 우선 산출하고, 이어서 Green 함수로 슬릿형 기공에 대한 Navier-Stokes 식의 해석해를 구하였다. 계면동전기적 유동에 의한 흐름전위를 관련된 물리화학적 인자들의 함수로 유도되는 해석적인 명확한 표현으로 제시하였다. 전기이중층, 표면전위, 그리고 기공벽면의 하전조건의 영향에 따른 유속분포와 흐름전위 변화를 고찰하였다 계산결과, 전기이중층 두께나 표면전위가 증가함에 따라 평균유속은 감소하는 반면에 흐름전위는 증가하였다.