• 한국건설관리학회:학술대회논문집
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• 한국건설관리학회 2008년도 정기학술발표대회 논문집
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• pp.725-728
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• 2008

플랜트 건설공사의 경우 최근 매우 복잡화, 전문화, 대형화되고 있고, 이에 따라 지속적으로 첨단기술접목과 함께 고부가가치 창출이 예상되고 있어 더욱 고도화된 사업관리계획과 더불어 전문인력 양성, 지식정보의 공유가 필수적으로 성장의 기본 요건으로 자리매김하고 있는 추세이다. 이에 따라 본 연구는 플랜트 건설산업의 경쟁력 강화를 위하여 관련 현황을 분석하고, 기업의 특성에 맞춘 유연한 Knowledge Management System(KMS) prototype을 구축, 웹 환경기반의 Portal에 연동한 시스템을 제시하고자 한다.

• Plant Biotechnology Reports
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• 제5권4호
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• pp.309-315
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• 2011

Rice grown in anaerobic waterlogged soil accumulates ammonium as a major source of nitrogen (N). We have compared the physiological symptoms of rice seedlings subjected to N-starvation stress with those receiving sufficient N, based on measurements of shoot/root length and weight and an analysis of protein expression patterns. N starvation marginally increased root growth but notably decreased shoot biomass. N uptake was reduced by >50% in the roots and shoots of N-starved seedlings. To better understand the mechanism of N starvation in rice, we performed a comparative proteome analysis of proteins isolated from rice leaves. Twenty-five differentially expressed proteins were analyzed by matrixassisted laser desorption/ionization time-of-flight (TOF) mass spectrometry and electron spray ionization quadrupole TOF. Functional analysis of the N-starvation response proteins suggested their involvement in protein synthesis and fate, metabolism, and defense. These results indicate that these proteins may play important roles in regulating the plant's complex adaptation responses for N use during N starvation. The proteins may be useful for further characterization of protein function in plant N nutrition.

• 한국지역지리학회지
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• 제4권1호
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• pp.77-98
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• 1998

본 연구는 제조업 중에서도 지역성장 및 연관산업에 대한 파급효과가 가장 큰 부문인 자동차 산업을 대상으로 새로운 조립공장 입지가 지역경제의 성장 및 발전에 미치는 영향을 경계지리학적 관점에서 논의했다. 우리 나라의 대표적 신산업지구로 발전하고 있는 광양만권의 율촌 산업단지를 사례로, 한국 최대의 자동차 조림기업인 현대자동차의 새로운 조립공장 입지가 광주 전남의 자동차산업 전반에 어떠한 영향을 미칠 것인지, 그리고 보다 미시적 수준에서 광양만권에 어떠한 파급효과를 초래할 것인지 탐색해보고자 했다. 노동집약적 특성과 자본 및 기술집약적 특성을 동시에 지니는 자동차 조립 및 부품생산 부문을 대상으로 첫째, 조립부문-부품생산부문의 공간적 비공간적 연계 특성을 규명하고 둘께, 광주 전남지역 자동차산업의 현 주소 및 이에 대한 신설 조립공장의 파급효과를 분석하며 셋째, 신설 조립공장 입지에 따른 광양만권 역내의 부품산업 발전 가능성을 전망함으로써 지역개발의 파급효과를 극대화할 수 있는 방안을 모색해보고자 하는 것이 본 연구의 목적이다.

• 대한의생명과학회지
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• 제23권2호
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• pp.64-72
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• 2017

The formation of brown colonies due to phenol oxidase activity on classic agar media containing natural material extracts of Helianthus annuus or on medium containing L-3,4-dihydroxyphenylalanine has been used to identify Cryptococcus species complex. In this study, various natural materials were used to develop a modified medium and to identify five major serotypes of Cryptococcus species complex. Serotypes A, D, and A/D were pigmented on medium using Perilla frutescens var. japonica Hara (PerJ agar) after a three-day incubation. Serotypes B and C were pigmented on PerJ agar after four- and five-day incubations, respectively. Growth time and pigmentation of the five serotypes occurred more rapidly on PerJ agar than on the other media. In addition, colony morphology, size, and pigmentation were specific by serotype. In conclusion, PerJ agar should be used in clinic settings to identify Cryptococcus species complex and its serotypes rapidly.

• 대한의생명과학회지
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• 제24권3호
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• pp.183-195
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• 2018

Cryptococcosis, which is caused by the Cryptococcus species complex (including Cryptococcus neoformans and Cryptococcus gattii), is well known as one of the most important medical problems. However, the of the Cryptococcus species complex is still limited to pneumonia and meningitis. In particular, the differences in virulence among the five major serotypes of the Cryptococcus species complex are not fully understood. To elucidate the virulence of the Cryptococcus species complex when it is disseminated hematogenously, rats were infected by different strains of the Cryptococcus species serotype, and their histopathological characteristics were compared after infection. The cumulative mortality ratio of rats infected with serotype B strain was slightly higher than in the other experimental groups. In addition, the average recovery of the Cryptococcus species complex from rats infected with serotype B strain was significantly higher than in the other groups in almost all organ samples except spleen. The recovery of the Cryptococcus species complex was associated with the severity of histopathological lesions, including bleeding, inflammation, and tissue damage in all organs. In rats infected with serotype B strain, the virulence was the most severe, especially in the lungs and liver. These results indicate that the pathophysiology of the Cryptococcus species complex infection differs according to serotype.

• The Plant Pathology Journal
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• 제20권4호
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• pp.258-263
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• 2004

Ripe fruit of pepper (Capsicum annuum) showed resistance to Colletotrichum gloeoporioides, but unripe fruit was susceptible. We previously isolated the PepTLP gene that induced in both unripe and ripe fruit by fungal infection and wound, and only in ripe fruit by jasmonic acid (JA) treatment. To examine further regulation of PepTLP, the action of specific agonist and antagonists of known signaling effector on the .PepTLP expression by fungal infection, wound, and JA was investigated. A similar dephosphorylation event negatively activated all the PepTLP expression in the ripe fruit by fungal infection, wound, and JA. The induction of PepTLP expression by wound is differentially regulated via phosphorylation and dephosphorylation step during pre- and post-ripening, respectively. In addition, the induction of PepTLP expression in the ripe fruit by wound and JA is differentially regulated via dephosphorylation and phosphorylation step, respectively. Only both wound and JA treatment has synergistic effect on the PepTLP expression in the unripe fruit. Both SA and JA treatments on the unripe fruit, and both wound or JA and SA on the ripe fruit could not do any effect on the expression of PepTLP. These results suggest that the induction of PepTLP expression is differentially regulated via complex regulatory system against fungal infection, wound, and JA treatment during pre- and post-ripening of pepper fruit.

• Journal of Plant Biotechnology
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• 제47권4호
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• pp.283-288
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• 2020

Although the evolution of Arabidopsis thaliana and humans diverged approximately 1.6 billion years ago, recent studies have demonstrated that protein function and cellular processes involved in disease response remain remarkably conserved. Particularly, γ-secretase, a multisubunit protein complex that participates in intramembrane proteolysis (RIP) regulation, is also known to mediate the cleavage of more than 80 substrates including the amyloid precursor protein (APP) and the Notch receptor. Although the genes (PS1/2, APH-1, PEN-2, and NCT) coding for the γ-secretase complex components are present in plant genomes, their function remains largely uncharacterized. Given that the deposition of 42 amino acid long amyloid-β peptides (hAβ₄₂) is thought to be one of the main causes of Alzheimer's disease, we aimed to examine the physiological effects of hAβ₄₂ peptides on plants. Interestingly, we found that Arabidopsis protoplast death increased after 24 h of exposure to 3 or 5 µM hAβ₄₂ peptides. Furthermore, transgenic Arabidopsis plants overexpressing the hAβ₄₂ gene exhibited changes in primary root length and silique phyllotaxy. Taken together, our results demonstrate that hAβ₄₂ peptides, a metazoan protein, significantly affect Arabidopsis protoplast viability and plant morphology.

• 식물분류학회지
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• 제43권1호
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• pp.34-45
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• 2013

마디풀과의 물여뀌 종집단(Polygonum amphibium L. complex)은 육상 및 수중 환경 모두에 서식할 수 있는 분류군으로, 서식 환경에 따라 다양한 형태 변이를 나타내어 현재까지 많은 분류군들이 기재되어 왔다. 아시아 및 북미산 107개체로부터 측정한 11개 형태형질을 사용하여 주성분분석을 수행한 결과, 본 종집단에서 존재하는 수생형 및 육생형 개체들은 모든 지역집단에서 잎의 형태 및 크기, 엽병의 길이 등에 의해 서로 구분되는 것으로 나타났다. 그러나, 동일 개체군 또는 동일 지역내에서 채집된 수생형과 육생형 개체들은 엽록체 DNA 4개 구간(matK, psbA-trnH IGS, rbcL-accD IGS, trnL-trnF)에서 완전히 동일한 염기서열을 공유하는 것으로 밝혀져 유전적으로는 분기되지 않은 것으로 추정되며, 따라서 본 종집단에서 나타나는 생육형간의 형태적 차이는 서식지 환경에 따른 개체 변이인 것으로 판단된다. 형태분석 및 엽록체 4구간 염기서열 유합자료의 계통분석 결과, 한국, 일본, 중국, 몽골, 극동 러시아 지역 등에 분포하는 아시아산 개체들은 북미지역집단 개체들 및 유럽의 영국산 개체와 형태적, 유전적으로 뚜렷이 구분되는 것으로 밝혀졌으며, 따라서 한반도산 개체들을 포함하는 아시아 지역집단 개체들은 P. amphibium의 하나의 변종(P. amphibium var. amurense Korsh.)으로 인식하는 것이 타당한 것으로 판단된다.

• 식물분류학회지
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• 제39권1호
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• pp.29-34
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• 2009

구절초 무리는 국내에 분포하는 국화속 중 흰색 또는 분홍색의 설상화를 가지는 특징으로 인해 다른 두 종과 구별되는데 외부형태학적 변이로 인하여 다양한 분류학적 견해가 제안되어 온 분류군이다. 본 연구에서는 지금까지 국화속의 종 및 종내 분류군을 인식하기 위해 이용되어 온 형태학적 형질 중 구절초 무리내의 종내 분류군을 인식하기 위한 특징의 일부로서 사용된 바 있는 잎의 두께에 주목하여 구절초 무리에 속하는 13개 집단과 근연종인 산국 및 감국의 4개 집단을 이용하여 잎의 구조적 특징을 확인하였다. 이를 위해 잎의 횡단절편을 제작하여 엽신의 두께와 세포층 수, 세포의 크기를 비교하였다. 잎의 두께를 확인해본 결과 산국, 감국, 구절초 무리 순으로 두꺼운 것으로 나타났으며 세 종 모두 9개 전후의 세포층으로 구성되어 있어 잎의 두께는 세포층 수가 아니라 세포 크기에 따라 달라지는 것으로 밝혀졌다. 구절초 무리 내에서는 집단 간에 잎의 형태 및 두께에서 다양한 변이를 보였는데 가는잎구절초, 바위구절초, 울릉구절초, 한라구절초 등 특히 제한적인 분포를 보이는 분류군들이 전국적인 분포를 보이는 분류군에 비해 상대적으로 두꺼운 잎을 가지는 것이 확인되었고, 이는 각 분류군이 고유의 서식환경에 적응한 결과로 생각된다.

• 한국토양비료학회지
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• 제51권2호
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• pp.128-141
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• 2018

Industrial complex releasing huge amounts of dusts, fumes and wastewater containing heavy metal(loid)s could be a source of heavy metal(loid)s pollution in arable soil. Heavy metal(loid)s pollution in arable soil adversely affect crops safety, subsequently human being. Hence, it is important to accurately assess the heavy metal(loid)s pollution in soil using pollution indices. The objectives of this study are 1) to compare assessment methods of heavy metal(loid)s pollution in arable soils located near industrial complex in Gyeongsang provinces and 2) to determine the relationship between concentration of plant available heavy metal(loid)s and chemical properties of soil. Soil samples were collected from 85 sites of arable lands nearby 10 industrial complex in Gyeongsang provinces. The average total concentration of all heavy metal(loid)s of the studied soils was higher than that of Korean arable soils but did not exceed the warning criteria established by the Soil Environmental Conservation Act of Korea. Only six sites of arable soils for the total concentration of As, Cu and Ni exceeded the warning criteria (As: $25mg\;kg^{-1}$, Cu: $150mg\;kg^{-1}$, Ni: $100mg\;kg^{-1}$). The contamination factor (CF) and geoaccumulation index ($I_{geo}$) of the heavy metal(loid)s in arable soils varied among the sampling sites, and the average values of As and Cd were relatively higher than that of other metals. Results of integrated indices of As and Cd in arable soils located near industrial complex indicated that some arable soils were moderately or heavily polluted. The plant available concentration of heavy metal(loid)s was negatively related to the soil pH and negative charge of soil. Available Cd, Pb, and Zn concentrations had relatively high correlation coefficient with pH and negative charge of soil when compared with other heavy metal(loid)s. Based on the above results, it might be a good soil management to control pH with soil amendments such as lime and compost to reduce phytoavailability of heavy metal(loid)s in arable soil located near industrial complex.