• The Plant Pathology Journal
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• v.31 no.2
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• pp.108-114
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• 2015

Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China, and several key pathogenic factors have been isolated. An yeast two-hybrid (Y2H) library is a very useful platform to further unravel novel pathogenic factors in C. lunata. To construct a high-quality full length-expression cDNA library from the C. lunata for application to pathogenesis-related protein-protein interaction screening, total RNA was extracted. The SMART (Switching Mechanism At 5' end of the RNA Transcript) technique was used for cDNA synthesis. Double-stranded cDNA was ligated into the pGADT7-Rec vector with Herring Testes Carrier DNA using homologous recombination method. The ligation mixture was transformed into competent yeast AH109 cells to construct the primary cDNA library. Eventually, a high qualitative library was successfully established according to an evaluation on quality. The transformation efficiency was about $6.39{\times}10^5$ transformants/$3{\mu}g$ pGADT7-Rec. The titer of the primary cDNA library was $2.5{\times}10^8cfu/mL$. The numbers for the cDNA library was $2.46{\times}10^5$. Randomly picked clones show that the recombination rate was 88.24%. Gel electrophoresis results indicated that the fragments ranged from 0.4 kb to 3.0 kb. Melanin synthesis protein Brn1 (1,3,8-hydroxynaphthalene reductase) was used as a "bait" to test the sufficiency of the Y2H library. As a result, a cDNA clone encoding VelB protein that was known to be involved in the regulation of diverse cellular processes, including control of secondary metabolism containing melanin and toxin production in many filamentous fungi was identified. Further study on the exact role of the VelB gene is underway.

• Journal of Fashion Business
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• v.1 no.2
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• pp.65-73
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• 1997

Over the past, the Korean Textile Industry has been able to establish as one of the most competent textile producer of the world. Today, however, with rapidly changing market requirements, the Korean Textile Industry is requested to improve systems for highvalue textile's market. In this regard, the purpose of this study is to propose suggestions for the strategy recommendations in the Korean Textile Design, researching on the highvalue-added Como Textile Design in Italy. As we analyzed them, the bases of their global success are as follows. 1) Participating in the making of the Global Trend in textile design market, Italian design companies lead to move international market influentially. 2) Dividing target depending on the country's textile market, Italian design companies build up their global marketing capability to increase design sale's effect. 3) Organizing and Participating in the international textile design show and fair, Italian companies attempt to set up Italian image creation as the global advertising. 4) Ensuring an adequate supply of human resource, Industry, Labor Unions, Government and Academy have a cooperative relationship to assist each other. In the basis of the above mentioned, we can suggest the strategies to accelate improvement and development in the Korean Textile and Textile Design Industry. 1) It needes to establish a monitoring system for current information in the International Textile Design Industry. 2) It needs to start building up Korean textile companies global marketing capability to produce adaptable design in market's request. 3) It needs to study and to find our traditional motifs continuosly for creating modern design following the recent Design Trend. Finally, It needs to have a close relationship between industry and education to establish human resource for the Korean Textile Industry's future success in the global market.

• Computers and Concrete
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• v.25 no.1
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• pp.67-73
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• 2020

Traditionally used analytical approach to predict the fatigue failure of reinforced concrete (RC) structure is generally conservative and has certain limitations. The nonlinear finite element method (FEM) offers less expensive solution for fatigue analysis with sufficient accuracy. However, the conventional implicit dynamic analysis is very expensive for high level computation. Whereas, an explicit dynamic analysis approach offers a computationally operative modelling to predict true responses of a structural element under periodic loading and might be perfectly matched to accomplish long life fatigue computations. Hence, this study simulates the fatigue behaviour of RC beams with finite element (FE) assemblage presenting a simplified explicit dynamic numerical solution to show computer aided fatigue behaviour of RC beam. A commercial FEM package, ABAQUS has been chosen for this complex modelling. The concrete has been modelled as a 8-node solid element providing competent compression hardening and tension stiffening. The steel reinforcements are simulated as two-node truss elements comprising elasto-plastic stress-strain behaviour. All the possible nonlinearities are duly incorporated. Time domain analysis has been adopted through an automatic Newmark-β time incremental technique. The program consists of twelve RC beams to visualize the real behaviour during fatigue process and to obtain the reliability of the study. Both the numerical and experimental results indicate a redistribution of stresses along the time and damage accumulation of beam which severely affect the serviceability and ultimate capacity of RC beam. The output of the FEM analysis demonstrates good match with the experimental consequences which affirm the efficacy of the computer aided model. The controlled fatigue damage evolution at service fatigue load limits makes the FE model an efficient tool in predicting high cycle fatigue behaviour of RC structures.

• Journal of the military operations research society of Korea
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• v.29 no.1
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• pp.88-111
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• 2003

This paper is to suggest strategic options for improving the export performance of the plant and construction industry. The overall objective of this study is to explore the feasibility of a sustained export performance in the plant and overseas construction industry by an analysis of its international competitiveness and technological competence. The empirical work of this study relates to a chosen sample of Korean and non-Korean firms in the plant and construction industry sector. Primary data was collected through a comprehensive questionnaire survey administered to plant and construction firms in twelve countries, including Korea. The actual number of firms for which full information at a sufficient level of disaggregation was obtained was 62, a response rate of 44.6％. Of these, 42 firms were drawn from Korean plant and construction firms and the remaining 20 firms from 11 other countries. The structure of responding firms by industry shows a total of 29 plant exporters and 33 construction firms. Data analysis was carried out using SPSS statistical technique such as Multiple Regression in order to examine the linear relationship among variables. The findings of the study indicate that export success and failure in plant and construction export markets is determined by firm size and by various qualitative variables. The high export volume (export success) of Plant and construction exporters is more strongly influenced by mutual economic cooperation and number of employees than by sales volume and competent knowledge of the plant and construction markets. It was also found that weak political and diplomatic relation between countries, low sales volume and lack of bid experience have an adverse effect and represent serious barriers to exports.

• Clinics in Shoulder and Elbow
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• v.9 no.1
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• pp.73-82
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• 2006

Purpose: The purpose of the present study was to evaluate the diagnostic efficacy of CT arthrography (CTA) in the assessment of various shoulder pathologies, compared with MR arthrography (MRA) and MRI with arthroscopic correlation. Materials and Methods: CTA in 84 patients, MRA in 70 patients, and MRI in 27 patients were obtained. A radiologist interpreted each image for 5 pathologies: Bankart, SLAP, Hill-Sachs lesion, full-thickness, and partial-thickness rotator cuff tear. Detailed arthroscopic reports were compared with CTA, MRA, and MRI. The sensitivity, specificity, predictive values, and accuracy were calculated. The agreement between each diagnostic modality and arthroscopy was calculated. Diagnostic efficacy was assessed by the areas under the receiver operating characteristic (ROC) curves. Results: The diagnostic values of all three imaging groups were comparable to each other for Bankart, SLAP, Hills-Sachs, and full-thickness cuff tear lesions, but those of CTA were lower than MRI and MRA for partial-thickness cuff tears. The areas under the ROC curves for CTA, MRA, and MRI were not significantly different for all pathologies, except for partial-thickness cuff tears. Conclusion: CTA was equally competent to MRA or MRI in demonstrating Bankart, Hill-Sachs lesions, SLAP, and full thickness rotator cuff tears but not as efficient in diagnosing partial thickness rotator cuff tears.

• Journal of Korean society of Dental Hygiene
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• v.14 no.4
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• pp.453-462
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• 2014

Objectives : The purpose of the study is to investigate the education, certification system, and extent of duty of dental hygienist between Korea and five developed countries including United States, Canada, England, Australia, and Japan. Methods : Internet based access to five developed countries was made and the analysis was done for the definition of occupation, main duties, similar occupations, education policy, job descriptions, license certification system, standards of duty, Q & A management, current status of obtaining certification, and scope of work. Results : United States has a two-year associate degree(AS) and a four-year bachelor degree(BS). Canada has two to three years degree and a two-year course is accepted in england and Australia. In the meanwhile, Korea and Japan have two-year and four-year educational courses. The duty of dental hygienists includes the prevention education for dental health and continuing dental health care. Most of the dental hygienists in Korea and Japan play the assistant roles for the dental surgeons. United States has national board examinations including written examinations, practical examinations, and computer assisted examination. Written and practical examinations are also conducted in Korea. England and Australia have the recognized educational organizations for qualification. Conclusions : Problem based and problem solving skills are the most important in dental hygienist education in Korea. The training of highly competent dental hygienists must be done for the improvement of dental hygiene in Korea in the near future.

• Journal of Educational Research in Mathematics
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• v.9 no.1
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• pp.263-278
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• 1999

Computer with various powerful functions has profound potential for mathematics instruction and learning. As computer technology progress, its applicability to mathematics education become more comprehensive. Not only its functional development but various psychological positions also changed the way computer technology utilized in mathematics education. In behaviorist's perspective, computer viewed as a teaching machine and constructivist viewed computer as microworld where students could explore various mathematical contents. Both theoretical positions emphasized individual aspect of learning because behaviorist tried to individualize learning using computer and constructivist focused on the process of individual construction. But learning is not only a individual event but also a social event. Therefore we must take social aspect into account. This is especially important when it comes to computer based learning. So far, mathematics loaming with computer weighed individual aspect of loaming. Even in microworld environment, learning should be mediated by teacher and collaborative learning activities. In this aspect, the roles of teacher and peers are very important and socio-cultural perspective sheds light on the computer based learning. In socio-cultural perspective, the idea of scaffold is very important in learning and students gradually internalize the social dimension and scaffolding is gradually faded. And in the zone of proximal development, teacher and more competent peers guide students to formulate their own understanding. In sum, we must take following points into account. First of all, computer should not be viewed as a medium for individualized teaming. That is, interaction with computer should be catalyst for collaborative activities with peers. So, exploration in computer environment has to be followed by small group activities including small group discussion. Secondly, regardless of the role that computer would play, teacher should play a crucial role in computer based learning. This does not mean teacher should direct every steps in learning process. Teacher's intervention should help student construct actively. Thirdly, it is needed to conceptualize computer in learning situation as medium. This would affect learning situation and result in the change of pre-service and in-service teacher training. Computer to be used effectively in mathematics classroom, researches on assessment of computer based learning are needed.

• Health Policy and Management
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• v.23 no.3
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• pp.224-232
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• 2013

Background: This study was a comparative analysis of the major curriculum for 2-year, 3-year, and 4-year colleges of the department of health administration in Korea. Methods: Among 45 Korean colleges registered with the department of health administration as of 2012, finally 25 colleges were selected by establishing the department of health administration more than 5 years ago, excluding government's financial support, and taking regional locations into account. Results: First, generally 2-year colleges was not the relationships between organizational philosophy/purposes and major course in comparison to those of 3-year or 4-year colleges. Second, the composition of credits in the major course appears to be higher for 3-year, 2-year, and 4-year colleges, respectively. Third, subjects related to basic medicine and medical records were included more often in 2-year, 3-year, and 4-year colleges, respectively, and health policy issues were covered more in 4-year, 3-year, and 2-year colleges, respectively. Fourth, the number of students per full-time professor in 2-year colleges was much higher than that in 3-year and 4-year colleges, and the employment rate of graduates for 4-year colleges was found to be higher than that for 2-year and 3-year colleges. Conclusion: Although this study has limitations with regard to the selection of research subjects, it provides baseline data for establishing the health administration required for training competent future professionals.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.10a
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• pp.31-50
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• 2000

Almost all currently available retroviral vectors based on murine leukemia virus (MLV) contain one or more viral coding sequences Because these sequences are also present in the packaging genome, it has been suggested that homologous recombination may occur between the same nucleotide sequence in the packaging genome and the vector, resulting in the production of replication competent retrovirus (RCR). Up until now, it has been difficult to completely remove viral coding sequences since some were thought to be involved in the optimum function of the retroviral vector. For example, the gag coding sequence present in almost all available retroviral vectors has been believed to be necessary for efficient viral packaging, while the pol coding sequence present in the highly efficient vector MFG has been thought to be involved in achieving the high levels of gene e(pression. However, we have now developed a series of reroviral vectors that are absent of any retroviral coding sequences but produce even higher levels of gene expression without compromising viral titer. In these vectors the intron and exon sequences from heterologous cellular or viral genes are present, When compared to the well blown MLV-based vectors, some of these newly developed vectors have been shown to produce significantly higher levels of gene expression for a longer period. In an experimental system that can maximize the production of RCR, our newly constructed vectors produced an absence of RCR. These vectors should prove to be safer than other currently available retroviral vectors containing one or more viral coding sequences

• Archives of Reconstructive Microsurgery
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• v.22 no.1
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• pp.7-12
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• 2013

Purpose: Breast reconstruction with lower abdominal tissue can produce the best outcome with acceptable rates of long-term complication. However, for cases in which sufficient abdominal tissue is not available, an superior gluteal artery perforator (SGAP) flap can be considered as the next option for autologous breast reconstruction. Materials and Methods: Among a total of 63 women who underwent breast reconstruction with free autologous tissue transfer from July 2010 to April 2011, SGAP flap was performed for four patients. In two cases, patients did not have enough abdominal tissue for sizable breast reconstruction. In another case, the patient had a long abdominal scar due to donor hepatectomy of liver transplantation. In the last case, which was a revisional case after radiation necrosis of a previous pedicled transverse rectus abdominis musculocutaneous (TRAM) flap, a large amount of healthy skin and soft tissue was needed. SGAP flap was elevated in lateral decubitus position. The internal mammary vessels were used for recipient vessels in all cases. Results: Breast reconstruction was performed successfully in all four cases without flap loss. Donor site complication was not observed, except for one case of seroma. The shape of the reconstructed breast was satisfactory in all patients. Conclusion: SGAP flap is an excellent alternative option for the TRAM or deep inferior epigastric artery perforator flap for breast reconstruction. In terms of narrower width, harder consistency of soft tissue, and shorter pedicle, it is clear that the SGAP flap is less competent than the TRAM flap. However, in cases where abdominal tissue is not available, SGAP flap is the only way of providing a large amount of healthy tissue.