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New Insights for Febrile Urinary Tract Infection (Acute Pyelonephritis) in Children

  • Lee, Kyung-Yil
    • Childhood Kidney Diseases
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    • v.20 no.2
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    • pp.37-44
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    • 2016
  • Although asymptomatic bacteriuria, cystitis, and acute pyelonephritis (APN) have been categorized as urinary tract infections (UTIs), the immunopathogenesis of each disease is different. APN shows an age predilection; the majority of children (over 70-80%) with APN are under 1-2 years of age, with a male predominance. After 1-2 years of age, female predominance has been reported. This finding suggests that the immature immune state of infancy may be associated with the pathogenesis of APN. Escherichia coli is the most common etiologic agent; other uropathogens associated with UTIs originate from the host and comprise normal flora that are continuously altered by environmental factors. Therefore, uropathogens may have characteristics different from those of extraneous bacterial pathogens. Although antibiotic-resistant uropathogens, including extended-spectrum beta-lactamase-producing strains, are increasing in Korea and worldwide, treatment failure is rare in immune-competent children. The immunopathogenesis of APN remains unknown. Intact bacteria may not be the causative substances in renal cell injury; rather, smaller substances produced during bacterial replication may be responsible for renal cell injury and scarring. Moreover, substances from host cells such as proinflammatory cytokines may be involved in renal cell injury. A dimercaptosuccinic acid scan is used to detect the site of bacterial replication in the renal parenchyma, and may be influenced by the size of the focus and the stage of APN. Traditional aggressive studies used to identify vesicoureteral reflux after the first episode of APN have been modified because of rare cases of chronic kidney disease in patients with recurrent UTI.

Characterization of Complemented Mutants in Pseudomonas fluorescens and Cloning of the DNA Region Related in Antibiotic Biosynthesis (길항세균 Pseudomonas fluorescens의 Complemented Mutant에 대한 특성조사에 및 길항물질 유전자 Cloning)

  • Kim, Young;Cho, Yong-Sup
    • Korean Journal Plant Pathology
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    • v.10 no.3
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    • pp.151-156
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    • 1994
  • Pseudomonas fluorescens produces the antibiotic, 2,4-diacetylphloroglucinol (Phl), which promotes plant growth by inhibiting bacteria and fungi. Cosmids (genomic library) were mobilized into Phl-nonproducing mutants through the triparental matings with pRK2013 as the helper plasmid at the frequency of 8.37$\times$10-4. Complemented mutants that showed antibiotic activity were selected among about 2,000 transconjugants. The complemented mutants were confirmed by acquired drug resistances (kanamycin and tetracycline). The antibiotic substances of wild type and complemented mutants showed the most excellent anti-bacterial activity. Inhibitory effects of complemented P. fluorescens against phytopathogenic fungi were equal to the parental strain. Complemented mutant and wild type of P. fluorescens were causal microbes of fungal morphological abnormalities. Complemented mutants in potato dextrose agar supplemented with bromothymol blue also showed restoration of glucose utilization as wild type. Plasmids of complemented mutants were isolated from transconjugant sand transformed into competent cells of E. coli DH5$\alpha$. The plamid DNA was reisolated from transformed E. coli DH5$\alpha$.

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A Study on the Inspection and Certification System of Organic Production of Agricultural Products and Foodstuffs in EU - Germany - (EU의 유기농식품 검인증시스템 연구 -독일을 중심으로-)

  • Yoo, Duck-Ki
    • Korean Journal of Organic Agriculture
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    • v.16 no.1
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    • pp.59-89
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    • 2008
  • The Council Regulation (EEC) No 2092/91 of 24 Juni 1991 on organic production of agricultural products and indications referring thereto on agricultural products and foodstuffs. This Regulation describes the legal framework for agricultural products and foodstuffs obtained organically. The Regulation establishes a harmonized framework for the production, labelling and inspection of agricultural products and foodstuffs in order to increase consumer confidence in such products and ensure fair competition between producers. This article presents a study about inspection and certification system of organic production of agricultural products and foodstuffs in Germany. In order to guarantee respect for the rules of production, the Regulation provides for an inspection system to ensure that operators who produce, prepare or store organic products or import them from third countries notify the competent private and public authorities in the Member States of their activities. These inspection authorities must, at the very least, ensure application of the minimum inspection and precautionary measures laid down in Annex III to the Regulation. For the production of meat, the Regulation states that the Member States must guarantee the traceability of products throughout the production, processing and preparation chain.

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A Numerical Study on the Aerodynamic Characteristics of a Bus-Like Bluff Body - Effect of Turbulence Model and Discretisation Scheme - (버스형상 무딘물체의 공력특성에 관한 수치해석적 고찰 - 난류모델과 이산화법의 영향 -)

  • 김민호;국종영;천인범
    • Transactions of the Korean Society of Automotive Engineers
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    • v.11 no.3
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    • pp.115-123
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    • 2003
  • With the advent of high performance computers and more efficient numerical algorithms, computational fluid dynamics(CFD) has come out as a modem alternative for reducing the use of wind tunnels test in automotive engineering. However, in spite of the fact that many competent researchers have made all their talents in developing turbulence model over since the past dozen or more years, it has been an important impediment in using the CFD effectively to design machinery and to diagnose or to improve engineering problems in the industry since the turbulence model has been acting as the Achilles' tendon in aspect of the reliability even to this time. In this study, Reynolds-averaged Wavier-Stokes equations were solved to simulate an incompressible turbulent flow around a bus-like bluff body near ground plane. In order to investigate the effect of the discretisation schemes and turbulence model on the aerodynamic forces several turbulence models with five convective difference schemes are adopted. From the results of this study, it is clear that choice of turbulence model and discretisation scheme profoundly affects the computational outcome. The results also show that the adoption of RNG $k-\varepsilon$ turbulence model and nonlinear quadratic turbulence model with the second order accurate discretisation scheme predicts fairly well the aerodynamic coefficients.

General Transcription Factors and Embryonic Genome Activation

  • Oqani, Reza K.;Kang, Jung Won;Lin, Tao;Lee, Jae Eun;Jin, Dong-Il
    • Reproductive and Developmental Biology
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    • v.38 no.1
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    • pp.41-52
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    • 2014
  • Embryonic genome activation (EGA) is a highly complex phenomenon that is controlled at various levels. New studies have ascertained some molecular mechanisms that control EGA in several species; it is apparent that these same mechanisms regulate EGA in all species. Protein phosphorylation, DNA methylation and histone modification regulate transcriptional activities, and mechanisms such as ubiquitination, SUMOylation and microRNAs post-transcriptionally regulate development. Each of these regulations is highly dynamic in the early embryo. A better understanding of these regulatory strategies can provide the possibility to improve the reproductive properties in mammals such as pigs, to develop methods of generating high-quality embryos in vitro, and to find markers for selecting developmentally competent embryos.

An Analysis of Mediation Function between Concrete and Abstract of the Model (구체와 추상을 연결하는 모델의 중재기능 분석)

  • Shin Eun Ju;Lee Chong Hee
    • Journal of Educational Research in Mathematics
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    • v.15 no.1
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    • pp.1-19
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    • 2005
  • There have been raised the question that students have been of no interst in mathematcs and incompetent for solving real world problem because students have been recognized mathematcs as abstract knowldege. We research whether students' modeles developed in modeling activity can mediate between concrete and abstract. The analysis of our case study revealed that students' modeles aren't decontextualized abstraction but is located in situated abstraction that is a network connecting between concrete and abstract. Thus, these modeles are a tool mediating between concrete and abstract. Also, students' modeling activities can provide students with the opportunity of being competent for solving real world problem.

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A Stipulation Based Sources Insertion Multilevel Inverter (SBSIMLI) for Waning the Component Count and Separate DC Sources

  • Edwin, Jose S;Titus, S
    • Journal of Electrical Engineering and Technology
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    • v.12 no.4
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    • pp.1519-1528
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    • 2017
  • The paper proposes a well structured, component count waned single phase multilevel inverter (MLI) topology, which drives three different modules viz. Stipulation Based Sources Insertion (SBSI) module, Level Count Increasing (LCI) module and Inter-Linking H-Bridge (ILHB) module. The SBSI module confronts the number of basic sources needed in series/parallel to achieve required magnitude for any particular level. The LCI possesses an offsetting dc source and opuses to increase the number of levels and the ILHB module links the SBSI and LCI modules. A developed Hybrid Pulse Width Modulation (HPWM) strategy has PWM pulses for the switches of LCI module while the switches of the remaining two modules function at fundamental switching frequency. A fifteen level version of the proposed stipulation based sources insertion MLI (SBSIMLI) topology is simulated in MATLAB R2010a and a prototype of the similar specifications is constructed to validate the performance by experimental results. The comparison between the developed SBSIMLI topology and the competent topologies shows many interesting facts.

A Study of Stage setting(image) device based on Animation : Ani-Stage (애니메이션을 이용한 영상무대장치에 대한 연구 : 애니 스테이지(Ani-Stage))

  • Min, Kyung-Mi
    • Cartoon and Animation Studies
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    • s.11
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    • pp.105-120
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    • 2007
  • This treatise has defined afresh the stage setting(image) device with use of animation which is known as virtual studio, cyber studio and all that sort of things as a word 'Ani-Stage' and has also adjusted it's generative background. It will be suggest that the established stage setting device with some confined techniques need to be grafted other various animation techniques together in order to make room for 'Ani-Stage-oriented' and will be offer a device able to make free use of composition angles, simplification of composite process free from limited factors. The purpose of this treatise is that raise up the recognition on ani-stage of the specialistic competent person who playing a role in the sphere of animation and that give a new definition about the stage setting device based on animation.

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In vitro-growth and Gene Expression of Porcine Preantral Follicles Retrieved by Different Protocols

  • Ahn, J.I.;Lee, S.T.;Park, J.H.;Kim, J.Y.;Park, J.H.;Choi, J.K.;Lee, G.;Lee, E.S.;Lim, J.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.7
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    • pp.950-955
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    • 2012
  • This study was conducted to determine how the isolation method of the porcine preantral follicles influenced the following follicular growth in vitro. Mechanical and enzymatical isolations were used for retrieving the follicles from prepubertal porcine ovaries, and in vitro-growth of the follicles and the expression of folliculogenesis-related genes were subsequently monitored. The enzymatic retrieval with collagenase treatment returned more follicles than the mechanical retrieval, while the percentage of morphologically normal follicles was higher with mechanical retrieval than with enzymatic retrieval. After 4 days of culture, mechanically retrieved, preantral follicles yielded more follicles with normal morphology than enzymatically retrieved follicles, which resulted in improved follicular growth. The mRNA expression of FSHR, LHR Cx43, DNMT1 and FGFR2 genes was significantly higher after culture of the follicles retrieved mechanically. These results suggest that mechanical isolation is a better method of isolating porcine preantral follicles that will develop into competent oocytes in in vitro culture.

A Qualitative Study on the Experience of Mothers Sending Their Children to English Kindergarten (자녀를 영어유치원에 보내는 어머니들의 경험에 대한 연구)

  • Yi, Yul-E;Yang, Sung-Eun
    • Korean Journal of Human Ecology
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    • v.18 no.5
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    • pp.985-994
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    • 2009
  • This research is exploratory in considering the unique socio-cultural context of South Korea, where the present government is responding to the demand for English language training. The study asked the question: What do mothers experience when sending their young children to a private English institute, so called English kindergarten, instead of a regular preschool? A qualitative approach was used to analyze the in-depth interviews with 19 mothers who sent their young child to an English kindergarten. Mothers stated that their young child needs to be a competent English speaker. The mothers expected that an English kindergarten would prepare their child better for the elementary school English curriculum than a regular preschool. The study revealed that English kindergartens symbolized the precedence and the privileges of the elite because of their high tuition fees, native-speaker teachers, and small class sizes. The mothers showed a sense of pride and vicarious satisfaction from sending their child to an English kindergarten. However, the mothers recognized that English kindergartens put more emphasis on cognitive learning instead of the social development of children. It was almost impossible for mothers to communicate with the native-speaker teachers about their child. The mothers seemed to overlook their child's struggle to adapt to an English Kindergarten. The findings of the study raise issues concerning the boom of teaching young children English in Korea.