• Title/Summary/Keyword: Community engineering

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Molecular Analysis of the Microorganisms in a Thermophilic CSTR used for Continuous Biohydrogen Production (연속수소생성에 사용되는 고온 CSTR 내의 미생물의 분자적 분석)

  • Oh, You-Kwan;Park, Sung-Hoon;Ahn, Yeong-Hee
    • KSBB Journal
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    • v.20 no.6
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    • pp.431-437
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    • 2005
  • Molecular methods were employed to investigate microorganisms in a thermophilic continuous stirred tank reactor(CSTR) used for continuous $H_2$ production. The reactor was inoculated with heat-treated anaerobic sludge and fed with a glucose-based medium. Denaturing gradient gel electrophoresis showed dynamic changes of bacterial populations in the reactor during 43 days of operation. Gas composition was constant from approximately 14 days but population shift still occurred. Populations affiliated with Fervidobactrium gondwanens and Thermoanaerobacterium thermosaccharolyticum were dominant on 21 and 41 days, respectively. Keeping pH of the medium at 5.0 could suppress methanogenic activity that was detected during initial operation period. $CH_4$ and mcrA detected in the samples obtained from the reactor or inoculum suggested the heat treatment condition employed in this study is not enough to remove methanogens in the inoculum. PCR using primer sets specific to 4 main orders of methanogens suggested that major $H_2$-consuming methanogens in the CSTR belong to the order Methanobacteriales.

Thermophilic Biohydrogen Production from Glucose with a Long-term Operation of CSTR (CSTR의 장기운전을 통한 포도당으로부터의 고온 수소생산)

  • Ahn, Yeong-Hee;Oh, You-Kwan;Park, Sung-Hoon
    • KSBB Journal
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    • v.20 no.6
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    • pp.425-430
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    • 2005
  • Thermophilic $H_2$ was produced for 1 year using a bench-scale continuous stirred tank reactor(CSTR). The CSTR was inoculated with anaerobically digested sludge after heat treatment and fed with a glucose-based medium. The reactor showed relatively short start-up period(30 days) and high maximal $H_2$ yield(2.4 mol $H_2/mol$ glucose). Keeping pH 5.0 or less suppressed methanogenic activity. Bacteria affiliated with Thermoanaerobacterium thermosaccharolyticum kept being dominant from approximately 40 days as determined by DGGE. Environmental perturbation(pH or temperature) caused the decrease of biomass concentration in the reactor and the instability of reactor performance, $H_2$ production rate and $H_2$ yield. The unstable performance was accompanied with high concentration of lactate in the effluent. Taken together, the poor recovery of CSTR after perturbations could be partly explained by low biomass concentration and/or metabolic shift of the major population in the CSTR.

Effect of Reducing the Odor of Food Wastes Using Effective Microorganism (EM) (유용미생물을 활용한 음식물쓰레기의 악취저감 효과)

  • Kim, Ha-Na;Yim, Bongbeen;Kim, Sun-Tae
    • Journal of Korean Society of Environmental Engineers
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    • v.38 no.4
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    • pp.162-168
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    • 2016
  • The aim was to investigate the effect of reducing the odorous and complex odor released during the decomposition of food wastes using effective microorganism (EM) as a function of time at $20^{\circ}C$ and $35^{\circ}C$. The variation of total microbial counts and dominant species counts in EM and leachate produced during food wastes decomposition was also observed. In general, the cumulative concentration of sulfur compounds ($H_2S$, $CH_3SH$) and complex odor released during food wastes decomposition increased with increasing elapsed time. The nitrogen compounds ($NH_3$, trimethyl amine), however, was not observed in all samples. The addition of EM in food wastes resulted in the reduction of concentration of sulfur compounds and complex odor, in spite of the increase of $CH_3CHO$ concentration. The dominant microbial species detected in EM were Lactobacillus species(Lactobacillus rhamnosus and Lactobacillus casei). In the leachate produced during food wastes decomposition, however, the various microbial community alternative to that detected in EM was observed. The EM could be potentially useful as a tools for reducing odor induced from the food waste decomposition process.

Enhancement of Denitrification Capacity of Pseudomonas sp. KY1 through the Optimization of C/N ratio of Liquid Molasses and Nitrate (액상 당밀과 질산성 질소의 C/N 비율에 따른 Pseudomonas sp. KY1의 탈질 능력 및 그 최적비율에 관한 연구)

  • Lee, Kyuyeon;Lee, Byung Sun;Shin, Doyun;Choi, Yongju;Nam, Kyoungphile
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.9
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    • pp.654-659
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    • 2013
  • This study was conducted to identify an optimal ratio of carbon to nitrogen (C/N ratio) for denitrification of nitrate using molasses as an external carbon source. A series of batch and column tests was conducted using an indigenous bacterium Pseudomonas sp. KY1 isolated from a nitrate-contaminated soil. For the initial nitrate-nitrogen concentration of 100 mg-N/L, batch test results indicated that C/N ratio of 3/1 was the optimal ratio with a relatively high pseudo-first-order reaction constant of $0.0263hr^{-1}$. At C/N ratio of 3/1, more than 80% of nitrate-nitrogen concentration of 100 mg-N/L was removed in 100 hrs. Results of column tests with a flow velocity of 0.3 mL/min also indicated that the C/N ratio of 3/1 was optimal for denitrification with minimizing remaining molasses concentrations. After 172 hrs of column operation (35 pore volumes) with an influent nitrate-nitrogen concentration of 100 mg-N/L, the effluent met the drinking water standard (i.e., 10 mg $NO_3$-N/L).

Physical Disturbance Improvement Evaluation and Habitat Suitability Analysis by Stable Channel Design (안정하도 설계에 따른 물리적 교란개선 평가와 서식적합도 분석)

  • Lee, Woong Hee;Choi, Heung Sik
    • Ecology and Resilient Infrastructure
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    • v.3 no.4
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    • pp.285-293
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    • 2016
  • This study conducted the evaluations of stable channel and physical disturbance improvement for optimal construction of river structures by focusing on Wonju River. A stable slope was analyzed sectionally for stable channel design, and in order to satisfy the stable slope, river structure improvement scenarios were deduced. Accordingly, through physical disturbance improvement evaluation for each scenario, the study extracted the optimal scenario for stable channel design and physical disturbance improvements. The changes in physical habitat were analyzed when river structure improvements were made for stable channel design and physical disturbance improvement. Zacco koreanus, an indicator fish of the soundness of the aquatic ecosystem, was selected as a restoration target species by investigating the community characteristics of fish fauna and river environments in the Wonju River. The habitat suitability was investigated by the PHABSIM model with the habitat suitability index of Zacco koreanus. The results of the prediction evaluation showed a slight decrease in habitat suitability and weighted usable area. However, it was not influenced by the improvements in the river structure. The study suggested river structure arrangement methods that can improve physical soundness and safety of Wonju River, and confirmed that there were no effects to the changes in the physical habitat.

Analysis of water quality improvement efficiency using constructed wetland in a coastal reservoir (연안 담수호 수질오염 방지를 위한 인공습지의 수질정화효율 분석)

  • Hong, Jungsun;Maniquiz-Redillas, Marla C.;Ham, Jong-Hwa;Kim, Lee-Hyung
    • Journal of Wetlands Research
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    • v.18 no.3
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    • pp.292-300
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    • 2016
  • Diverse and comprehensive countermeasures were established to prevent water pollution in coastal areas such as constructed wetlands(CW).This study was conducted to assess the water quality improvement through CW constructed along the shoreline of Hwaseong coastal reservoir. The CW is located in Hwaseong-si, Gyeonggi-do and consisted of a forebay and a wetland. The CW was monitored twice during rainy days and 10 times during dry days. The monitoring results indicated that in and out flowrates were highly correlated with COD and TN loads. COD, TN and TP concentrations in the forebay was lower during dry days than rainy days. However, concentration and mass removal efficiencies of COD, TN and TP were greater during rainy days. In addition, the volume flowing into the CW was less compared to the outflow during rainy days indicating that the CW efficiently reduced the runoff volume. The overall pollutant removal efficiency of the CW were at least 50% for TSS, 20 to 35% for TP, and 26 to 94% for TN. The data gathered may be used to improve the pollutant removal efficiency of the system in the future.

Properties of a Hexane-Degrading Consortium (Hexane 분해 혼합균의 특성)

  • Lee Eun-Hee;Kim Jaisoo;Cho Kyung-Suk
    • Microbiology and Biotechnology Letters
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    • v.33 no.3
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    • pp.215-221
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    • 2005
  • It was characterized the hexane biodegradation and mineralization using a hexane-degrading consortium, and analyzed its bacterial community structure by 16S rDNA PCR-DGGE (denaturing gradient gel electrophoresis). The specific growth rate (${\mu}_{max}$) of the hexane-degrading consortium was 0.2 $h^{-1}$ in mineral salt medium supplemented with hexane as a sole carbon source. The maximum degradation rate ($V_{max}$) and saturation constant ($K_{s}$) of hexane of the consortium are 460 ${\mu}mol{\cdot}g-DCW^{-1}{\cdot}h^{-1}$ and 25.87 mM, respectively. In addition, this consortium could mineralize $49.1{\%}$ of $^{14}C$-hexane to $^{14}CO_2$, and $43.6{\%}$ of $^{14}C$-hexane) was used for the growth of biomass. The clones isolated from the DGGE bands were closely related to the bacteria which were capable of degrading pollutants such as oil, biphenyl, PCE, and waste gases. The hexane-degrading consortium obtained in this study can be applied for the biological treatment of hexane.

Analysis on Statistical Relationship between Groundwater Quality and Geology (지하수 수질과 지질의 통계학적 상관성 분석)

  • Kim, Jong-Tae;Park, Sang-Joo;Kang, Mee-A;Choo, Chang-Oh;Jeong, Gyo-Cheol
    • The Journal of Engineering Geology
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    • v.17 no.3
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    • pp.445-453
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    • 2007
  • The purposes of this study lie on understanding characteristic water-rock interaction mechanisms of groundwater in the Geochang, Hapcheon, and Changryeong areas and establishing correlation between geological factors and groundwater chemistry by statistical tools. Based on the statistical results from the relationship between rock types and groundwater chemistry, fluorine is correlated with Fe and Al in the diorite area, whereas it is correlated with Cl and $NO_3-N$ in the andesite area. Hardness, total solids, and $SO_4$ show that they are closely related to each other in all rock types, especially with highest relationship in sedimentary rocks. Although it is generally acknowledged that TDS increases with depths of aquifer due to the long term water-rock interaction, no remarkable relationship between well depth and water chemistry is found in this study. It appears that hydrogeological system in the study area may be complex or the path of water-rock interaction is inconsistent with well depth.

Stock Price Prediction Using Sentiment Analysis: from "Stock Discussion Room" in Naver (SNS감성 분석을 이용한 주가 방향성 예측: 네이버 주식토론방 데이터를 이용하여)

  • Kim, Myeongjin;Ryu, Jihye;Cha, Dongho;Sim, Min Kyu
    • The Journal of Society for e-Business Studies
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    • v.25 no.4
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    • pp.61-75
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    • 2020
  • The scope of data for understanding or predicting stock prices has been continuously widened from traditional structured format data to unstructured data. This study investigates whether commentary data collected from SNS may affect future stock prices. From "Stock Discussion Room" in Naver, we collect 20 stocks' commentary data for six months, and test whether this data have prediction power with respect to one-hour ahead price direction and price range. Deep neural network such as LSTM and CNN methods are employed to model the predictive relationship. Among the 20 stocks, we find that future price direction can be predicted with higher than the accuracy of 50% in 13 stocks. Also, the future price range can be predicted with higher than the accuracy of 50% in 16 stocks. This study validate that the investors' sentiment reflected in SNS community such as Naver's "Stock Discussion Room" may affect the demand and supply of stocks, thus driving the stock prices.

A Study on Regionalization of Parameters for Sacramento Continuous Rainfall-Runoff Model Using Watershed Characteristics (유역특성인자를 활용한 Sacramento 장기유출모형의 매개변수 지역화 기법 연구)

  • Kim, Tae-Jeong;Jeong, Ga-In;Kim, Ki-Young;Kwon, Hyun-Han
    • Journal of Korea Water Resources Association
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    • v.48 no.10
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    • pp.793-806
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    • 2015
  • The simulation of natural streamflow at ungauged basins is one of the fundamental challenges in hydrology community. The key to runoff simulation in ungauged basins is generally involved with a reliable parameter estimation in a rainfall-runoff model. However, the parameter estimation of the rainfall-runoff model is a complex issue due to an insufficient hydrologic data. This study aims to regionalize the parameters of a continuous rainfall-runoff model in conjunction with a Bayesian statistical technique to consider uncertainty more precisely associated with the parameters. First, this study employed Bayesian Markov Chain Monte Carlo scheme for the estimation of the Sacramento rainfall-runoff model. The Sacramento model is calibrated against observed daily runoff data, and finally, the posterior density function of the parameters is derived. Second, we applied a multiple linear regression model to the set of the parameters with watershed characteristics, to obtain a functional relationship between pairs of variables. The proposed model was also validated with gauged watersheds in accordance with the efficiency criteria such as the Nash-Sutcliffe efficiency, index of agreement and the coefficient of correlation.