• Journal of Microbiology and Biotechnology
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• 제19권8호
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• pp.781-786
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• 2009

Small interfering synthetic double-stranded RNA (siRNA) was applied to suppress the expression of the human cytotoxic-T-Iymphocyte antigen 4-immunoglobulin (hCTLA4Ig) gene transformed in transgenic rice cell cultures. The sequence of the 21-nucleotide siRNA was deliberately designed and synthesized with overhangs to inactivate the expression of hCTLA4Ig. The chemically synthesized siRNA duplex was combined with polyethyleneimine (PEl) at a mass ratio of 1:10 (0.33 ${\mu}g$ siRNA:3.3 ${\mu}g$ PEl) to produce complexes. The siRNA complexes (siRNA+PEI) were labeled with Cy3 in order to subsequently confirm the delivery by fluorescent microscopy. In addition, the cells were treated with sonoporation at 40 kHz and 419W for 90 s to improve the delivery. The siRNA complexes alone inhibited the expression of hCTLA4Ig to 45% compared with control. The siRNA complexes delivered with sonoporation downregulated the production of hCTLA4Ig to 73%. Therefore, we concluded that the delivery of siRNA complexes into plant cells could be enhanced successfully by sonoporation.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1113-1119
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• 2007

Human bone marrow-derived mesenchymal stem cells (hBMMSCs) must differentiate into osteogenic cells to allow for successful bone regeneration. In this study, we investigated the effects of different combinations of three soluble osteogenic differentiation-inducing factors [L-ascorbic acid (AC), ${\beta}$-glycerophosphate (${\beta}G$), and bone morphogenic protein-2 (BMP-2)] and the presence of a hydroxyapatite (HA) substrate on hBMMSC osteogenic differentiation in vitro. hBMMSCs were cultured in medium containing various combinations of the soluble factors on culture plates with or without HA coating. After 7 days of culture, alkaline phosphatase (ALP) activity, calcium deposition, and osteoprotegerin (OPG) and osteopontin (OPN) expression were measured. The effects of individual and combined factors were evaluated using a factorial analysis method. BMP-2 predominantly affected expression of early markers of osteogenic differentiation (ALP and OPG). HA had the highest positive effect on OPN expression and calcium deposition. The interaction between AC, ${\beta}G$, and HA had the second highest positive effect on ALP activity.

• Animal cells and systems
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• 제8권1호
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• pp.49-55
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• 2004

In vertebrates, multisubunit cofactors regulate gene expression through interacting with cell-type- and gene-specific DNA-binding proteins in a chromatin-selective manner. ADD1/SREBP1c regulates fatty acid metabolism and insulin-dependent gene expression through binding to SRE and E-box motif with dual DNA binding specificity. Although its transcriptional and post-translational regulation has been extensively studied, its regulation by interacting proteins is not well understood. To identify cellular proteins that associate with nuclear form of ADD1/SEBP1c, we employed the GST pull-down system with Hela cell nuclei extract. In this study, we demonstrated that Ku proteins interact specifically with ADD1/SREP1c protein. GST pull-down combined with peptide sequencing analysis revealed that Ku80 binds to ADD1/SREBP1c in vitro. Additionally, western blot analysis showed that Ku70, a heterodimerizing partner of Ku80, also associates with ADD1/SREBP1c. Furthermore, co-transfection of Ku70/Ku80 with ADD1/SREBP1c enhanced the transcriptional activity of ADD1/SREBP1c. Taken together, these results suggest that the Ku proteins might be involved in the lipogenic and/or adipogenic gene expression through interacting with ADD1/SREBP1c.

• 제어로봇시스템학회논문지
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• 제19권6호
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• pp.513-519
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• 2013

The current vision-based approaches for emotion recognition, such as facial expression analysis, have many technical limitations in real circumstances, and are not suitable for applications that use them solely in practical environments. In this paper, we propose an approach for emotion recognition by combining extrinsic representations and intrinsic activities among the natural responses of humans which are given specific imuli for inducing emotional states. The intrinsic activities can be used to compensate the uncertainty of extrinsic representations of emotional states. This combination is done by using PRMs (Probabilistic Relational Models) which are extent version of bayesian networks and are learned by greedy-search algorithms and expectation-maximization algorithms. Previous research of facial expression-related extrinsic emotion features and physiological signal-based intrinsic emotion features are combined into the attributes of the PRMs in the emotion recognition domain. The maximum likelihood estimation with the given dependency structure and estimated parameter set is used to classify the label of the target emotional states.

• Journal of Plant Biotechnology
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• 제4권2호
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• pp.71-76
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• 2002

In order to achieve repetitive somatic embryogenesis in cacao (Theobroma cacao L.), callus derived from floral tissues were continuously cultured in a medium containing 2,4-D. In 5% of the explants, repetitive somatic embryogenesis was observed after 8 weeks and maintained in a globular stage for several weeks. This is the first report showing repetitive somatic embryogenesis in cacao. The calli were bombarded with a plasmid containing $\beta$-glucuronidase (gus) as reporter gene. Two week old calli showed the high average number of cells expressing the us gene. The effect of osmotic agents (mannitol, sorbitol and sucrose) on gene expression was evaluated. Pre-treatment during 16 h with 0.25 M mannitol revealed an improvement in gene expression. The potential utilization of the repetitive embryogenesis, combined with osmotic treatment, is discussed as an alternative to achieve stable transgenic cacao plants.

• 한국콘텐츠학회논문지
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• 제14권9호
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• pp.476-483
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• 2014

본 논문은 국내 표정 연구에 적합한 얼굴 표정 이미지를 제작하는 것에 목적을 두었다. 이를 위해서 1980년대 태생의 한국인의 표준 형상에 FACS-Action Unit을 결합하여, KSFI(Korean Standard Facial Image) AU set를 제작하였다. KSFI의 객관성을 확보하기 위해 6가지 기본 감성(슬픔, 행복, 혐오, 공포, 화남, 놀람) 이미지를 제작하여, 감성 별 인식 정확률과 얼굴 요소의 감성인식 기여도를 평가하였다. 실험 결과, 정확률이 높은 행복, 놀람, 슬픔, 분노의 이미지의 경우 주로 눈과 입의 얼굴 요소를 통해 감성을 판단하였다. 이러한 연구 결과를 통해 본 연구에서는 표정 이미지의 AU 변경할 수 있는 KSFI 콘텐츠를 제안하였다. 향후 KSFI가 감성 인식률 향상에 기여할 수 있는 학습 콘텐츠로서의 역할을 할 수 있을 것으로 사료된다.

• 한국실내디자인학회논문집
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• 제15권2호
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• pp.81-90
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• 2006

This treatise makes clear not only a structural methodology of 'the conceptual blending theory' using in a linguistics could be applied to the similar process in a hybrid design but also both methods include the same meaning construction in the sense of a mutual educational system. Both methods have something in common that they are fundamentally focused on a metaphorical expression which implies poetic messages to be concentrated on a formal structure. Therefore, the purpose of this treatise is to examine the structural characteristics in the hybrid design in order to analyze the rhetorical expressions(metaphor, allegory) as symbols of communication. One of the those is intertextuality that expresses metaphorically by blending and borrowing codes and another is hypertextual space where various texts twines around each other making brand-new and diverse organizations, as the combined allegory with a number of hidden expression. Ultimately, it is important that this approach could verify whether it deals the illogical present state of a spacial form or structure with a kind of mechanism of a 'conceptual blending theory' or not.

• Journal of Microbiology and Biotechnology
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• 제10권5호
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• pp.690-693
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• 2000

Efficient intron deletion with the correct splicing of the two exons of the human proinsulin gene was accomplished by a novel stepwise method using genomic DNA [5]. The two exons were separately amplified in two steps, using the second step primers that incorporated additional bases complementary to the other exon. The fragments were combined in a third PCR reaction. Cloning and sequencing of the PCR product demonstrated the correct splicing of the two exons. Expression studies, using the pET9a vector, revealed a protein band with the correct size with respect to human proinsulin as confirmed by SDS-PAGe and Western blot. Proinsulin concentration was estimated to be around 200 mg per liter culture, expressed as inclusion bodies. Protein secretion to the culture medium and periplasmic space was achieved by cloning in the pEZZ18 vector.

• 한국의류학회지
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• 제29권11호
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• pp.1444-1453
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• 2005

In this study, I tried to analyze the characteristics of modem punk fashion by applying characteristics of modem cultural phenomena. The method of this study is the qualitative study: I analyzed collections from 1996 to 2003 by suggesting an analysis frame based on bibliological studies and applying internet photo materials to the analysis frame. As a result, modem punk fashion has the following characteristics. First, it tends to promote things that are entirely different from the previous expression methods by using the overlapping of texts combined with things that are heterogeneous. Second, the scope of its decoration has been expanded with the aid of the expression method, in which the objects that had not been used as decoration previously have been transformed into media. Third, the appearance of non-mainstream group who has suggested the anti-mainstream thought that violates the uniformed beauty of punk group has expanded the scope of classes that accept the punk fashion. Lastly, clothes, wigs and accessories have been commercialized through the permanent modification that used to signify resistance and disgust such as the expression methods of image replicas using tattoo, piercing, and transformation of hair. In doing so, punk fashion has been generalized and popularized. Such modem punk fashion reflects the change of society such as social transformation that is occupied by images thanks to its complexity and pluralism and the development of public media.

• 비교문화연구
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• 제33권
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• pp.263-286
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• 2013

Is this a right way in politics that attitude of Japanese scholars to separate Natsume Soseki from the expansionism of pre-war Japan to protect 'sanctity'? Nowadays, most Japanese scholars are regarded to share the desire that minimize the memory of the behavior of Japanese Imperialism in East Asia, such as Korea, China, etc. Furthermore, 'the desire to minimize' inescapably concluded in avoidance, concealment, at last the temptation of deliberate misleading. Until now, the controversy about the Natsume Soseki's travel to Korea and Manchuria has repeated in defence and criticism surrounding the self-awareness and recognition of others of Natsume Soseki, making the expression in a record of Natsume's travel as the subject of study, for example, the degrading expression about Chosun people and scorn for Chinese and Russian. This paper will investigate that Natsume's travel is the political practice which is combined with the desire for the empire, focusing on the political context in the action of journey of Natsume and its contents other than the expression itself.