• Animal cells and systems
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• 제6권4호
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• pp.327-333
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• 2002

Among the silk glands in the black widow spider Latrodectus mactans, the ampullate one is the most predominant gland in both sexes, and is com-posed of three functional parts - excretory duct, storage ampulla and convoluted tail regions. This experiment was performed using mechanical pulling stimulation with electric motor equipment to reveal a correlation between silk usage and silk producing system in this poisonous spider. The mature secretory products in glandular epithelium are closely packed and appear as electron-opaque spherical vesicles. A part of the vesicles with fine fibrillar paracrystalline texture seems to store some proteins which will function at the time of final assembly into fibrils. Most of the secretory silk products which originated from the rough endoplasmic reticula of the glandular epithelial cells are grown by fusion with surrounding small vesi-cles. However, the Golgi complex does not seem to play an important role in this process of secretion. According to progressive maturation of secre-tory silk product, these granules are progressively filled with a fine fibrillar material, and thus appear much more electron-dense than those of earlier states. When the secretory product is extruded from the glandular cavity, the epithelium is rapidly changed to a thinner layer of tall columnar cells with less definitive cell membranes. After extruding there ave a few secre-tory droplets within these cells, thus causing this region to stain much lighter.

• Applied Microscopy
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• 제14권2호
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• pp.29-37
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• 1984

난소절제한 흰쥐에서 $17{\beta}$-estradiol과 progesterone을 장기간 주사햐여 자궁상피의 형태적 변화를 전자현미경으로 관찰하였다. 난소절제 후 자궁상피는 막성구조물과 지방구 기타의 구조물을 포함하는 vacuole의 출현이 특이적으로 나타났다. 상피는 낮은 입방형이며 세포 유리면에 소수의 짧은 microvilli를 볼 수 있었다. Estradiol투여시 상피는 높은 윈주형을 나타내며, 분비계는 비교적 잘 발달되고, 세포 유리면에 소수의 긴 microvilli도 관찰되었다. Progesterone 처리시의 상피는 세포 첨단부에 다수의 vacuole과 핵하부에 다수의 지방구의 집적이 관찰되었다. 이상의 결과로 보아 자궁상피는 형태학적 및 기능적인 상태에서 estrogen과 Progesterone 양자에 의해 변화를 받으며 이들 호르몬은 상피세포에 대해 독특한 영향을 나타낸다고 생각된다.

• 대한수의학회지
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• 제38권3호
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• pp.450-454
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• 1998

We have studied Meckel's diverticulum(MD) of the duck(Anas platyrhynchos platyrhyncos, Linne) by histological and immunohistochemical methods. Because MD were first observed in 2 weeks after hatching, tissues were taken from 2 weeks to 32 weeks after hatching groups. MD were observed as like any parts of intestinal tract that composed with simple columnar epithelium and numerous mucosal gland especially, cecum except that many lymphocytes were exist in this study. Also a number of bovine chromogranin(BCG)-, serotonin, and somatostatin(SOM)-immunoreactive cells were observed in epithelium and submucosal gland in this study, so it could be suggest that the MD of the duck serve as some digestive and lymphatic functions.

• Applied Microscopy
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• 제15권2호
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• pp.80-88
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• 1985

The tentacular epithelial cells of Cipangopaludina chinensis were studied with electron microscope. The tentacular epithelium consists of columnar supporting cells, numerous glandular cells and ciliated cells. Glandular cells are classified into three types; type I (mucous metachromasia cell), type II(mucous goblet cell) and type III. Ciliated cells are subdivided into two types; type I ciliated cell has cilia with typical axoneme(9+2), and type II ciliated cell has cilia with unusual axoneme.

• Anatomy and Cell Biology
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• 제51권4호
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• pp.299-301
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• 2018

Killian-Jamieson diverticulum is a permanent protrusion of anterolateral proximal esophagus through anatomically weak muscular gap, known as Killian-Jamieson area, into adjacent area. During a routine educational dissection, we found a well-defined lateral diverticulum just inferior to the transverse fibers of the cricopharyngeus muscle in a Korean male cadaver. It had a dimension of $1.8{\times}1.4{\times}1.0cm$ with two types of epithelial cells, stratified squamous and simple cuboidal to low-columnar epithelium, and attenuated and haphazardly arranged muscle fibers. No epithelial dysplasia or malignant transformation was identified except ulcerative changes. Although Killian-Jamieson diverticulum is a very rare disease, clinicopathological aspects should be considered.

• Animal cells and systems
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• 제3권3호
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• pp.253-258
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• 1999

The present study describes histochemical properties of the mucosubstances in the pedal sole of abalone, in relation to two main additional functions known to date. Only neutral mucosubstances were demonstrated in the lateral epithelium of the pedal sole of the abalone, however, two distinct types of cells, one containing neutral and the other containing acidic mucosubstances, were identified in the ventral epithelium. The epithelial mucocytes distributed throughout both epithelial and subepithelial glands located in the ventral subepithelial region showed a mixture of neutral and acidic mucosubstances. However, acidic mucosubstances were found in larger amounts than neutral ones in epithelial mucocytes, and vice versa in subepithelial glands. Among these acidic mucosubstances, sulphated substances were dominant. Lesser amounts of carboxylated substances also occurred together with low levels of neuramic and sialic acid. Collectively, the notable morphological differences between abalone and other gastropoda were observed in the ventral surface of the foot, which showed a tall columnar epithelium, peripherally concentrated mucocytes, and subepithelial glands. Mucosubstances are presumably produced by these cells and may also play a role in clinging to some substances to support abalones in addition to the main functions of lubrication and protection.

• 한국패류학회지
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• 제11권2호
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• pp.135-145
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• 1995

A morphological andk histochimical study on the amterior tintacular antenna of Korean sulg, Incilaria fruhstorferi was conducted under the light microscopic observations. The histological sturctures of the antenna were apparently divided into three parts such as the epithelium, the connective tissues and the muscular layers. The cells forming the antenna were classified into several types on the basis of their morphological and histochemical characteristics. The simple columnar epithelium cotering the whole antenna was composed of supporting cells, sensory neurons and type-a clear cells. The connective tissue was consisted of dispersed large cells, type-b clear cells and 7 types of secretory cills such as type-A, type-B, type-F, thpe-G, type-H, type-J and type-K. The large cells found in the form of group situated only in the stalk of the antenna. The large cells possessed relatively small nuclei as compared with their cytoplasm. The cytoplasm positively reacted upon alcian blue, and the nucleus was PASpositive. The type-a and type-b clear cells which were irregular in shape showed no evident reaction against various stains employed in the present study. The secrtory cells were observed mainly in the connective tissues and in the muscular layers. Histochemical components of the type-A, type-B and K were identified as acid mucopolysaccharides and those of type-F and H were neutral mucopolysaccharides. The muscular layders supporting the epithelium possessed the type-B and F secretory cells which were also observed in the connective tissues.

• Journal of Chest Surgery
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• 제12권1호
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• pp.9-13
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• 1979

A case of bronchogenic cyst arising from an accessory lobe in a 7-month old baby was encountered at Seoul University Hospital. On exploration, right lung consisted of 4 lobes and a huge cyst was confined to an accessory lobe, which had no bronchial connection. The cyst was filled with jelly-like thick mucus; inner surface was glistening and smooth. Microscopically, the cyst was lined with pseudostratified columnar epithelium. Bronchogenic cyst, moreover, which arising from an accessory lobe is very rare and interesting. And so, case report and review of literature was made.

• 생명과학회지
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• 제8권5호
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• pp.582-588
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• 1998

징거미 새우의 정소는 한 쌍의 관으로 되어 있고, 정소의 중앙 후반부에서 기원된 수정관은 제 5보각의 기부에 위치한 생식공까지 연결되어 있다. 수정관은 기부, 나선부, 말단부, 사정관으로 구분되었다. 수정관은 simple columnar ephithelium, high columnar ephithelium, 종주근과 환상근으로 이루어져 있다. AG는 수정관의 말단부에서 사정관에 걸쳐 위치하고 있다. AG의 형태는 불규칙한 세포 덩어리로 되어져 있고, hematoxyline에 약하게 염색되는 염색질과 둥글고 커다란 핵을 가진다. 핵질과 세포질은 염기성 핵막을 경계로 쉽게 구분되나, 세포질과 세포질사이의 경계는 그 구별이 어렵다. 암컷의 복판에 부착된 정포는 호산성기질, 호염기성기질 그리고 호염기성기질에 싸여있는 한 쌍의 정자덩어리로 구성되어 있다. 성숙정자는 압정형태이고, 두부는 가운데 부분이 약간 오목한 원반형이고, 미부는 두부 중앙부 하단의 약간 볼록한 부분에서 연장된 하나의 spike를 이루고 있다. 정자의 크기는 두부의 직경이 약 8 $\mu$m, 미부의 길이가 약 10 $\mu$m였다.

• Applied Microscopy
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• 제15권2호
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• pp.98-110
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• 1985

This study was performed to clarify the morphological structures of the epithelia of the renal papilla, renal pelvis and ureter of the sheep (Ovis aries L.) through the light and scanning electron microscopes, Tissue specimens were taken from the renal papilla (common renal papilla and peripelvic column) and the renal pelvis (pelvis proper and pelvic pouch) of the kidney and the ureter. For the light microscopy, tissue blocks were fixed in 10 % neutral buffered formalin and embedded in paraffin wax, serially sectioned at a thickness of $6{\mu}m$. These sections were stained with hematoxylin-eosin and periodic acid-Schiff reaction. For the scanning electron microscopy, tissue blocks were prefixed in 1% glutaral-dehyde-1.5% paraformaldehyde solution and postfixed in 1% osmium tetroxide solution, dehydrated in graded alcohol, transferred to isoamyl acetate, and then dried by the critical point dryer (Polaron E 3000). These dried tissues were coated with gold and observed with a scanning electron microscope (JSM-35C), The results were as follows: The apex of the common renal papilla was lined with simple columnar epithelium having many microvilli on its luminal surface. Lateral portion of the papilla was lined with stratified epithelium $2{\sim}3$ layers thick, and its superficial cells were microvillar cells having many microvilli. The epithelium lining the peripelvic column was $1{\sim}2$ layers thick. The superficial layer was made of the microvillar cells, but a few microplica cells were appeared in the region near the pelvic pouch. The epithelium of the pelvic pouch was $1{\sim}2$ layered transitional type, and its superficial cells were microplica cells. The epithelia of the pelvis proper and ureter were $4{\sim}6$ layered transitional type, and their superficial cells were typical facet cells existing many round depressions and ridges of cell membranes of the luminal side.