• Title/Summary/Keyword: Column decomposing

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Antioxygenic and Peroxide-decomposing Activities of Antarctic Krill Lipids (Krill 지질(脂質)의 항산화성(抗酸化性)과 과산화물(過酸化物) 분해작용(分解作用))

  • Lee, Jong-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.3
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    • pp.326-333
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    • 1984
  • The oxidation rate of krill lipids is very slow and no peroxides are accumulated even after long storage. By means of various chromatographic techniques and mass spectrophotometry, the primary antioxidant has been identified as ${\alpha}$-tocopherol. The phospholipid fractions did not show any antioxidative activity but peroxide-decomposing properties of total lipids depended upon the phospholipid contents. The peroxide-decomposing activities of phospholipids were due to the presence of polar materials generated during the storage. The most peroxide-decomposing fractions of oxidized krill lecithin by DEAE-cellulose column chromatography was low-molecule fraction (mean molecular weight: 182) and high-molecule fraction (mean molecular weight: 1942) was the next. The separation of peroxide-decomposing properties from low-molecule fraction was achieved by partitioning between chloroform and methanol/water. The methanol/water fraction showed strong peroxide-decomposing activities and main component of this fraction was assumed hydroxyamine compounds derived from choline.

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Storey-based stability analysis of multi-storey unbraced frames

  • Liu, Y.;Xu, L.
    • Structural Engineering and Mechanics
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    • v.19 no.6
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    • pp.679-705
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    • 2005
  • This paper presents a practical method to evaluate the effective length factors for columns in multi-storey unbraced frames based on the concept of storey-based elastic buckling by means of decomposing a multi-storey frame into a series of single-storey partially-restrained (PR) frames. The lateral stiffness of the multi-storey unbraced frame is derived and expressed as the product of the lateral stiffness of each storey. Thus, the stability analysis for the multi-storey frame is conducted by investigating the lateral stability of each individual storey, which is facilitated through decomposing the multi-storey frame into a series of single-storey PR frames and applying the storey-based stability analysis proposed by the authors (Xu and Liu 2002) for each single-storey PR frame. Prior to introducing decomposition approaches, the end rotational stiffness of an axially load column is derived and rotational stiffness interaction between the upper and lower columns is investigated. Three decomposition approaches, characterized by means of distributing beam-to-column rotational-restraining stiffness between the upper and lower columns, are proposed. The procedure of calculating storey-based column effective length factors is presented. Numerical examples are then given to illustrate the effectiveness of the proposed procedure.

Optimized Geometric LDPC Codes with Quasi-Cyclic Structure

  • Jiang, Xueqin;Lee, Moon Ho;Gao, Shangce;Wu, Yun
    • Journal of Communications and Networks
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    • v.16 no.3
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    • pp.249-257
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    • 2014
  • This paper presents methods to the construction of regular and irregular low-density parity-check (LDPC) codes based on Euclidean geometries over the Galois field. Codes constructed by these methods have quasi-cyclic (QC) structure and large girth. By decomposing hyperplanes in Euclidean geometry, the proposed irregular LDPC codes have flexible column/row weights. Therefore, the degree distributions of proposed irregular LDPC codes can be optimized by technologies like the curve fitting in the extrinsic information transfer (EXIT) charts. Simulation results show that the proposed codes perform very well with an iterative decoding over the AWGN channel.

Peptide Production from the Washing Liquid of the Fish Paste of Alaska pollak (Theragria chalcogramma) by Immobilized Enzyme (고정화 효소를 이용한 명태고기풀 수세액으로부터 Peptide 생산에 관한 연구)

  • SHIN Suk-U;SUETSUNA Kunio
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.3
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    • pp.466-472
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    • 1997
  • Peptides separated from fish paste washing liquid of an Alaska pollak (Theragria chalcogramma) were purified and characterized. The fish paste washing liquid (supernatant) was separated by centrifugation of fish paste homogenate. The fish paste washing liquid of $0.5\%$ concentration was hydrolyzed for 24 hour at $50^{\circ}C$ by immobilized protease in bioreactor and decomposing liquid of protein having $50\%$ decomposing rate (OPA method) was obtained. The crude peptide fractions were obtained from this liquid by Dowex 50w $(H^+)$ column chromatograpy. Purified peptides (SP-fraction peptides) were fractionated by using SP-Sepadex C-25 $(H^+)$ column chromatography. Molecular weights and amino acid compositions of these peptides were estimated by Sephadex G-50 column chromatography and HPLC, respectively. when the washed peptides was eluated with $0.6\~0.9\%\;and\;1.2\~2.0\%$ of NaCl, peptides composed of weakly basic amino acids and strongly basic amino acid were respectively eluted. Molecular weights of each peptide fractions showed the broad distribution from 1,000 Da to 3,000 Da in the order of SP-4>SP-3>SP-2>SP-1. Peptides contained a large quantity of glycine, arginine, glutamic acid, and alanine in the washed peptide and its SP-tractions, respectively.

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Nitrogen Removal from Synthetic Domestic Wastewater Using the Soil Column (토양컬럼을 이용한 합성하수 중의 질소제거)

  • Cheong, Kyung-Hoon;Lim, Byung-Gab;Choi, Hyung-Il;Park, Sang-Ill;Moon, Ok-Ran
    • Journal of Environmental Science International
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    • v.16 no.6
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    • pp.707-714
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    • 2007
  • A laboratory experiment was performed to investigate nitrogen removal by the soil column. The addition of 20% waste oyster shell to the soil accelerated nitrification in soil column. The $NO_3^--N$ concentration in the effluent decreased with the decrease of HRT(Hydraulic Retention Time). When methanol and glucose added as carbon sources, the average removal rates of T-N(Total Nitrogen) were 82% and 77.9%, respectively. The $NO_3^--N$ removal by methanol supplementation in soil column can likely be attributed to denitrification. In continuous removal of nitrogen using the soil column, the COD(Chemical Oxygen Demand) and $NH_4^+-N$ removed simultaneously in organic matter decomposing column. The greater part of $NH_4^+-N$ was nitrified by the percolated through nitrification column, and the little $NH_4^+-N$ was found in the effluent. The T-N of 87.4% removed at HRT of 36 hrs in denitrfication column. Because of nitrified effluents from nitrification column are low in carbonaceous matter, an external source of carbon is required.

Purification and characterization of versatile peroxidase from Pleurotus ostreatus produced in a rotary draft tube bioreactor (회전식 통풍관 생물반응기로부터 생산된 느타리균의 다목적 과산화효소(VP) 정제 및 특성)

  • Hyo-Cheol Ha
    • Journal of Mushroom
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    • v.21 no.4
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    • pp.209-214
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    • 2023
  • In this study, Pleurotus ostreatus No.42 was cultured in glucose-peptone-yeast-wheat bran medium using a previously reported novel rotary draft tube bioreactor. Versatile peroxidase (VP), a lignin-degrading enzyme, was isolated from a pellet-type mycelium culture grown in the medium for seven days. The VP was purified by sequentially applying ultra-filtration, DEAE-Sepharose CL-6B column, and Mono Q column. SDS-PAGE analysis revealed the molecular weight of VP to be 36.4 KDa with an isoelectric point of 3.65. The amino acid sequence was confirmed as VTCATGQTT. The purified VP was observed to possess the property of not only oxidizing Mn ions but also decomposing veratryl alcohol, a non-phenolic compound. The catalytic ability of VP is a subject for future research.

Crystallization of a-Amylase and Protease of Aspergillus oryzae from Columm Chromatography (I) (칼럼크로마토그라피에 의한 아스퍼질러스 계통의 .alpha.-아미라제 및 프로테아제의 결정화 1)

  • 서항원
    • Korean Journal of Microbiology
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    • v.9 no.4
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    • pp.163-168
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    • 1971
  • Neutral protease which was obtained from a genus of Aspergilli as the crystal form were investigated for their purification and properties. The results of biochemical and enzymatic studies for their purification and properties in this enzyme were as follows. 1) On the wheat media containing 70%-water and $CaCo_{3}$, Aspergilus oryzae S.H.W. 131 is satisfactorily grown under the basic optimum conditions temperature $27^{\circ}C$- $30^{\circ}C$at relative humidity 100% for three days. 2) The enzyme solution extracted with water is successively purified through the passing on column of Asmti-177N for decolorization of it. And ion exchanger such as DEAAE Sphadex A-50 or Shepadex G-100 and fraction collector is necessary for the sepearte treatments of this enzyme. After washing it with organic solvents as aceton-EtOH, etc., it should be dried on the vacuum dryer at $40^{\circ}C$) The protease activity is determined by the amounts of amino acids, tyrosine. 4) The optimum pH of neutral protease is 6.0-8.0. 5) In effectively decomposing with this neutral protease, the optimum temperature is $35^{\circ}C$. 6) It is interesting that the amounts of metal ion affects the activity of neutral protease. For examples, if it were treated with manganic ion, its activity would be more effective than any other that.

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Studies on the Characteristics of Mineral Diluents Affecting the Decomposition of Sumithion in the Dust formulations (Sumithion 분제의 성분 변화에 영향을 미치는 증량제의 특성에 관한 연구)

  • Wuh K. D.;Han S. S.;Keum S. S.;Ahn S. H.;Lee C. N.
    • Korean journal of applied entomology
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    • v.10 no.2
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    • pp.77-83
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    • 1971
  • In order to find out the decomposing factors of Sumithion in mineral diluents, the physical and chemical properties of the diluents such as talcs, bentonites, diatomaceous earth and other clay minerals was studied in relation to the decomposition rate of Sumithion in dust formulations. The total base, moisture adsorption capacity, pH, specific surface, water contents, active $Fe^{++}$, ignition loss and cation exchange capacity were analysed as the properties of mineral diluents, and these properties were correlated with the stability of Sumithion in dust formulations. And in hope of finding out the main factors among those properties of diluents, the decomposed products of Sumithion in dust formulations prepared with standard Sumithion were separated by the methods of column chromatography and investigated by thin layer chromatography. The following results were otbained; 1. The total base, moisture adsorption capacity, specific surface, water contents, active $Fe^{++}$ and cation exchange capacity of mineral diluents were found to be highly effective on the rate of decomposition of Sumithion in dust formulations. 2. Decomposed products of Sumithion in dust formulations were found to be dimethylphosphorothionate, 3-methyl-4-nitro phenol and its derivatives. And one fraction was not dissolved in n-hexane and ethylether, but was soluble in methylalcohol and ethylalcohol. 3. The moat highly correlated properties of diluents with the decomposition rate of Sumithion in dust were found to be the total base and water contents. 4. In regard to the kind of diluents, it was found that the rate of decomposition of Sumithion in dust formulations was higher in order of bentonite, diatomaceous earth, kaolin and talc.

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