• Journal of the Computational Structural Engineering Institute of Korea
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• v.36 no.5
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• pp.307-314
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• 2023

This study aims to evaluate the blast performance of shear and flexure failure modes of reinforced concrete columns using finite-element analyses. To accomplish this goal, finite-element models of flexure- and shear-governed columns were developed and validated using previous experimental results. A blast simulation model was developed using a coupling-modeling method, and the modeling method was applied to the validated-column models. Blast responses were obtained for various blast loading scenarios, and the blast performance was determined using limits based on ductility and axial loading capacity.

• Transactions of the Korean Society of Mechanical Engineers
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• v.9 no.5
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• pp.606-612
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• 1985

On the stability of the Beck's column with a tip mass, the influence of the characteristics of the springs at the fixed end of the column are studied. The equations of motion and boundary conditions of this system are established by using the Hamiton's principle. On the evaluation of the stability of the column, t he effect of the shear deformation and rotatory inertial is considered in calculation. For the maintenance of the stability of the column, it is proved that the constant of the translational spring at the fixed end must be very large while th magnitude of the constant of the rotational spring at the fixed end has no effect. When the constants of the springs at the fixed end are small, it is also proved that the influence of the moment of inertial of the tip mass on the stability of the column are decreased and for the translational spring the degree of the decrease is more and more. Therefore it is found that the characteristics of the springs at the fixed end are very effective elements for the stability of the column when the columns subjected to a compressive follower force are designed.

• Journal of the Korean Geotechnical Society
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• v.31 no.9
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• pp.5-15
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• 2015

In this study, rainfall-induced slope stability and coupling effect are investigated using hydro-mechanical finite element model. This model is developed by formulating constitutive and coupled balance equations and is verified by comparing the numerical results with field matric suction. The homogeneous soil layer (soil column) and soil slope are modeled by this model, and the results of variation in matric suction, mean effective stress, porosity, displacement, factor of safety are compared with those of staggered analysis. It is found that the vertical and horizontal displacement from coupling analysis considering change in porosity is larger than that of staggered analysis. The displacement and matric suction from coupling analysis by rainfall infiltration can affect slope instability, which shows a progressive failure behavior. The lowest factor of safety is observed under short-term rainfall. This results confirm the fact that coupling analysis is needed to design soil slope under severe rain condition.

• Steel and Composite Structures
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• v.28 no.4
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• pp.471-482
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• 2018

The composite reinforced concrete and steel (RCS) structural systems have larger structural lateral stiffness, higher inherent structural damping, and faster construction speed than either traditional reinforcement concrete or steel structures. In this paper, four RCS subassemblies with or without the RC slab designed following a strong column-weak beam philosophy were constructed and tested under reversed-cyclic loading. Parameters including the width of slab and composite effect of the RC slab and beam were explored. The test results showed that all specimens performed in a ductile manner with plastic hinges formed in the beam ends near the column faces. The seismic responses of composite connections are influenced significantly by different width of slabs. Compared with that of the steel beam without the RC slab, it was found that the load carrying capacity of composite connections with the RC slab increased by 30% on average, and strength degradation, energy dissipation also had better performance, while the ductility of that were almost the same. Furthermore, the contribution of connection deformation to the overall specimen displacement was analyzed and compared. It decreased approximately 10% due to the coupling effect in the columns and beams with the RC slab. Based on the test result, some suggestions are presented for the design of composite RCS joints.

• Clinical and Experimental Reproductive Medicine
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• v.17 no.1
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• pp.71-80
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• 1990

New immunoassay systems for the detection of anti-sperm antibodies were developed. For this, sperm surface protein was purified by the immunoaffinity column prepared by the coupling of rabbit anti-human IgG antibodies to Sepharose-4B. Fraction eluted by tris-HCI buffer containing SDS showed a single band having molecular weight of about 60KD on electrophoresis. Enzyme HRP labelled goat anti-human IgG and chemiluminescence aminobutylethyl-isoluminol(ABEI) labelled rabbit anti-human IgG were used for ELISA and CIA, respectively. These two labelled conjugate bound well with human IgG. When serum dilution curves were made to titrate positive serums, two kinds of curves with steep and sluggish slopes were obtained Serum samples were categorized into 3 groups: positive, weak positive and negative based on slope of curve and O.D. values at 1:160 dilution of serum. When ELISA and CIA were compared to conventional method Kibrick test by the determinations of 62 male serums with different diagnosis, the results of ELISA and CIA agreed well, but both disagreed with that of Kibrick test. This study showed that purified sperm surface antigen can be used to develope solid-phase immunoassay systems such as ELISA and CIA which may eliminate the problems encounted the immobilization of living sperm in other tests.

• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1810-1818
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• 2015

For the synthesis of various pharmaceuticals, chiral alcohols are useful intermediates. Among them, (R)-ethyl-4-chloro-3-hydroxybutanoate ((R)-ECHB) is an important building block for the synthesis of L-carnitine. (R)-ECHB is produced from ethyl-4-chloro-3-oxobutanoate (ECOB) by a reductase-mediated, enantioselective reduction reaction. The Saccharomyces cerevisiae YOL151W reductase that is expressed in Escherichia coli cells exhibited an enantioselective reduction reaction toward ECOB. By virtue of the C-terminal His-tag, the YOL151W reductase was purified from the cell-free extract using Ni²⁺-NTA column chromatography and immobilized onto Ni²⁺-magnetic microparticles. The physical properties of the immobilized reductase (Imm-Red) were measured using electron microscopy, a magnetic property measurement system, and a zeta potential system; the average size of the particles was approximately 1 μm and the saturated magnetic value was 31.76 emu/g. A neodymium magnet was used to recover the immobilized enzyme within 2 min. The Imm-Red showed an optimum temperature at 45℃ and an optimum pH at 6.0. In addition, Bacillus megaterium glucose dehydrogenase (GDH) was produced in the E. coli cells and was used in the coupling reaction to regenerate the NADPH cofactor. The reduction/oxidation coupling reaction composed of the Imm-Red and GDH converted 20 mM ECOB exclusively into (R)-ECHB with an e.e._p value of 98%.

• Analytical Science and Technology
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• v.8 no.4
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• pp.539-543
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• 1995

The on-line SPE-HPLC coupling system was developed for the efficient separation and determination of trace pesticides, such as phenoxyacetic acids and esters, and triazines in aqueous solutions. By using the developed SPE-HPLC on-line system, the band broadening usually observed in single precolumn switching mode was greatly reduced, consequently, the quantitative determination of trace pesticides could be achieved, Besides, since most of the analytes preconcentrated by SPE column could be injected directly into HPLC system, the limit of detection can be improved down to ppt level.

• Archives of Pharmacal Research
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• v.23 no.5
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• pp.467-470
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• 2000

Five furanocoumarins including a new one were isolated from the root of Angelica dahurica by repeated silica gel column chromatography. Their chemical structures were determined to be isoimperatorin (1), oxypeucedanin hydrate-3"-butyl ether (2), imperatorin (3), knidilin (4), and oxypeucedanin hydrate (5). This represents the first study in which the compound 2 has been isolated and identified, The long-range coupling ($^{5}J$) in the $^1H$-NMR spectrum observed in the linear furanocoumarin skeleton was also investigated in detail.

• Journal of the Korean Society of Tobacco Science
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• v.4 no.2
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• pp.67-73
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• 1982

Essential oil components from tobacco powder were investigated as flavouring agent. The essential oil was isolated from tobacco powder by a simple distillation /extraction method The extracted essential oil was fractionated into basic, acidic and neutral groups. And the neutral group of essential oil was separated by column chromatography into 10 fractions. Above groups and fractions were tested for tobacco aroma and smoke aroma. The neutral group except most nonpolar fraction displays good flavouring properties which make them highly suitable for improving the flavour and aroma of tobacco and tobacco smoke. The most nonpolar fraction from neutral group was carefully investigated using preparative column, thin layer and gas chromatography ailed by GC/MS coupling. The major subfraction was identified as hydrocarbons on the basis of the IR spectrum. The 58 hydrocarbon components were identified by their mass spectra and was chromatographic retention times.

• Steel and Composite Structures
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• v.21 no.5
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• pp.1121-1144
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• 2016

This paper presents a displacement-based finite element procedure for second-order distributed plasticity analysis of planar steel frames with semi-rigid beam-to-column connections under static loadings. A partially strain-hardening elastic-plastic beam-column element, which directly takes into account geometric nonlinearity, gradual yielding of material, and flexibility of semi-rigid connections, is proposed. The second-order effects and distributed plasticity are considered by dividing the member into several sub-elements and meshing the cross-section into several fibers. A new nonlinear solution procedure based on the combination of the Newton-Raphson equilibrium iterative algorithm and the constant work method for adjusting the incremental load factor is proposed for solving nonlinear equilibrium equations. The nonlinear inelastic behavior predicted by the proposed program compares well with previous studies. Coupling effects of three primary sources of nonlinearity, geometric imperfections, and residual stress are investigated and discussed in this paper.