• Title/Summary/Keyword: Colorimetric method

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Camparision and Analysis about the Weighted-Ordinate Colorimetric Method and the Direct Colorimetric Method of Halftone Dot Printing Colors (망점색인쇄물의 분광측색법과 자극치직독측색법에 대한 비교분석)

  • SangNamLee
    • Journal of the Korean Graphic Arts Communication Society
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    • v.6 no.1
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    • pp.13-39
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    • 1988
  • Test colors are halftone dot printed at the rate of 10% halftone dot area by process inksblack, cyan, magenta and yellow ink. Reproduced colors are measured by the weighted-ordinate colorimetric method and the direct colorimetric method. The weighted-ordinate colorimetric method compare favorably with the direct colorimetric method, because the former has good regular changes of tristimuIus values, chromacity coordinates, excitation purities, value functions and has smaller errors of dominant and complementary wavelengths than the latter. All test colors classify with the Munsell renotation.tation.

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A Study for the Calculation of the Surface Chloride on the concrete by using Colorimetric method (비색판별법을 이용한 콘크리트의 표면염화물량 산정)

  • 이진우;이문환;서치호
    • Proceedings of the Korea Concrete Institute Conference
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    • 2001.11a
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    • pp.815-820
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    • 2001
  • This study is to set a new standard for using of the colorimetric method through grasping the character of the colorimetric method, and measuring the chloride concentration at the place of the change of color. Also, to predict chloride concentration around rebar and time reaching limit chloride concentration through measuring the chloride concentration of concrete surface by the colorimetric method and this study presents the new program of concrete degradation and diagnosis of the durability by salt damage. First the use of the colorimetric method was examinated, second the chloride concentration through slicing concrete submerged in artificial seawater for 6 months was measured. And the chloride concentration at the place of the change of color was calculated by using colorimetric method. Finally, the cloride concentration of the concrete surface was calculated.

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Application of Colorimetric Method for Evaluation of Apparent Chloride Diffusion Coefficient of Concrete (콘크리트 중 겉보기 염소이온 확산계수 추정을 위한 비색법의 적용)

  • 문한영;김홍삼;최두선;오세민
    • Proceedings of the Korea Concrete Institute Conference
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    • 2003.11a
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    • pp.541-544
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    • 2003
  • Chloride diffusivity is one of the important properties of concrete affecting the durability of a structure. The diffusivity for porous materials is determined conventionally by immersion in a solution. However, this method is complicate and time-consuming, often requiring months or years to obtain results. Thus, the application of colorimetric method to estimate the apparent diffusivity of chloride ion was verified in this study. The result reveals that the apparent diffusivity of chloride ion can be predicted to use colorimetric method. Additionally the colorimetric method is capable to predict the profile of chloride ion.

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Analysis of Dimethylamine and Trimethylamine in Fishes by Gas Chromatography

  • Oh, Myung-Cheol;Oh, Chang-Kyung;Kim, Sung-Hong;Kim, Soo-Hyun
    • Preventive Nutrition and Food Science
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    • v.2 no.3
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    • pp.197-201
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    • 1997
  • To develop a rapid analytical method of dimethylamine(DMA) and trimethylamine(TMA) in fish, the contents of DMA and TMA in squid(Illex illecebrosus and Sepiell maindroni), cod(Gadus marcrocephalus) and plaice (Paralichthys olivaceus) by gas chromatographic (GC) and colorimetric method were determined. Recoveries for DMA in fish were 86.8~102.5% by GC and 74.2~94.5% by colorimetric method, while those for TMA were 93.0~101.1% by GC and 62.9~117.5% by colorimetric method. The contents of DMA and TMA in fish by GC were 29.7~325.3mg/kg and 145.6~356.0 mg/kg, respectively, and these by colorimetric method were 20.0~241.2mg/kg and 139.1~304.3mg/kg, respectively. The analysis of DMA and TMA in fishes by GC after the solvent extraction was simpler and faster and showed better recovery than colorimetric method.

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Rapid Analytical Method of Nitrite and Nitrate in Fish by Ion Chromatography

  • Oh, Myung-Cheol;Oh, Chang-Kyung;Kim, Soo-Hyun
    • Preventive Nutrition and Food Science
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    • v.1 no.1
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    • pp.1-5
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    • 1996
  • Rapid analytical method was investigated to determine precursors of N-nitrosamine such an nitrite and nitrate in squid(Illex illecebrosus and Sepiell maindroni), codfish(Gadus marcrocephalus)and flatfish(Paralichthys olivaceus) by ion chromatography(IC) and colorimetric methods. Recoveries of nitrite and nitrate in fish tissues were 89~98.7% and 94.1~99.8% for IC, and 98.4~103.7% and 67.7~102.2% for colori-metric method, respectively. Using IC, nitrite was not detected and nitrate was 0.89~1.23mg/kg, while using colorimetric method, nitrite and nitrate were NO~0.08mg/kg and 0.3~0.42mg/kg, respectively. Therefore, the IC method showed better recoveries, and was applicable to extract nitrite and nitrate simultaneously, and is simpler compared with colorimetric method in analyzing nitrite and nitrate from fish tissues.

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Calculation of the Surface Chloride and Estimation for the Soundness of Embedded Rebar by Using Colorimetric Distinction Method (비색판별법을 이용한 콘크리트의 표면염화물량 산정 및 매립철근의 건전도 평가)

  • Lee, Mun-Hwan;Lee, Jin-Woo
    • Journal of the Korea Concrete Institute
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    • v.15 no.6
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    • pp.794-801
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    • 2003
  • As it is important to measure the degree of the deterioration and predict service life caused by chloride in concrete structure the methods of measuring chloride in the concrete is raised important problems. This study is to set a new standard for using of the colorimetric method through grasping the character of the colorimetric distinction method, and measuring the chloride content at the place discolored. Also, to predict chloride content around embeded bar and time reaching limit chloride concentration through measuring the chloride content of concrete surface by colorimetric distinction method and this study presents the new concept of concrete degradation and diagnosis of the durability by salt damage. According the results, it is possible to use colorimetric distinction method as simplified measurement to measure the fixed quantity of the chloride concentration. What is more, it would make calculation of concrete surface chloride had a wide fluctuation at the general environment extended. Also, it would be make estimating durability of reinforced concrete structures applied to the basic data.

Evaluation of the Antibacterial Effects of Phellinus baumii Extract on Methicillin-Resistant Staphylococcus aureus by Using Broth Microdilution Based on a Colorimetric Method

  • Shin, Kyeong Seob;Jo, Mi Young;Hong, Seung Bok
    • Biomedical Science Letters
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    • v.22 no.4
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    • pp.167-173
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    • 2016
  • The broth microdilution technique used to measure the minimal inhibitory concentration (MIC) of natural compounds against bacteria is problematic: it is difficult to visualize bacterial growth due to the color of the natural compound. Therefore, the use of a colorimetric method with a redox indicator by broth microdilution can simplify it and increase its objectivity. This study evaluated the usefulness of the colorimetric method in measuring the MIC of Phellinus baumii against methicillin-resistant Staphylococcus aureus (MRSA). The inhibition in disc diffusion method was observed from $8,192{\mu}g/mL$ P. baumii in all 10 MRSA isolates examined; however, the MIC ranges of the 10 MRSA isolates was $512{\sim}2,048{\mu}g/mL$ by broth microdilution using a colorimetric method; with the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) indicator. In addition, the MIC of P. baumii by broth microdilution using MTT as indicator yielded excellent results. However, the 2, 3, 5-triphenyltetrazolium chloride (TTC) results could not be determined due to the color of the TTC indicator. The MICs of four antibiotics against MRSA using MTT or TTC were equal to those determined by visual interpretation. In conclusion, to evaluate the antibacterial effects of a natural compound, the broth microdilution technique is considered to be better than the disc diffusion method. Moreover, to resolve the problems caused by the colors of natural compounds, a colorimetric method such as that using MTT may be very valuable.

Evaluation of a Diffusive Sampler for the Measurement of Formaldehyde using Colorimetric Method (흡광광도법을 이용한 포름알데히드 확산측정기의 평가)

  • Yim, Bong-Been;Kim, Sun-Kyu;Jung, Eui-Suk;Kim, Sun-Tae
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.6
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    • pp.606-613
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    • 2005
  • The badge-type diffusive sampler for the measurement of formaldehyde in indoor air using three types of colorimetric methods such as chromotrophic acid(CTA), 3-methyl-2-benzothiazolinone hydrazone(MBTH), and 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole(AHMT) method. The washing of the collection filter with several cleaning solution was effected with satisfactory results, regardless of the types of cleaning solutions. The concentrations of absorbance solution in each colorimetric method were experimentally determined by considering the sampling rates. The variation blank values in each colorimetric method was below 15%. As compared with CTA and AHMT methods, the reproducibility of MBTH method was excellent and was below 10% relative standard deviation. The collected formaldehyde mass and time-weighted concentration had a good correlation (correlation coefficient > 0.93). The limit of detection and limit of quantitation, and minimum sampling time were closely correlated to the sampling rates for the measurement of formaldehyde in each method.

Direct Colorimetric Assay of Microcystin Using Protein Phosphatase

  • Oh, Hee-Mock;Lee, Seog-June;Kim, Jee-Hwan;Park, Chan-Sun;Yoon, Byung-Dae
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.6
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    • pp.418-421
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    • 2000
  • A new direct colorimetric assay of microcystin in water and algal samples is proposed consisting of two procedures as follows: 1) the elimination of phosphorus in the sample and concentration of microcystin using a C(sub)18 cartridge, 2) the detection of the released phosphorus by the ascorbic acid method and determination of protein phosphatase (PP) inhibition by microcystin. The optimum amounts of phosphorylase ${\alpha}$ and PP-1 in 50 ${\mu}$L concentrated sample were 50$\mu\textrm{g}$/50${\mu}$L buffer and 1.0unit/50${\mu}$L buffer, respectively, for the best assay. The pH for the maximum activity of PP-1 was 8. The minimum detectable concentration for this method was about 0.02$\mu\textrm{g}$/L, which is sufficient to meet the proposed guideline level of 1$\mu\textrm{g}$ microcystin/L in drinking water. Consequently, it would seem that the proposed direct colorimetric assay using PP is a rapid, easy, and convenient method for the detection of microcystin in water and algal samples.

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Studies on the Colorimetric Determination of Drugs with Phosphomolybdic Acid. I. Colorimetric Determination of Ampicillin with Phosphomolybdic acid

  • Lee, Wang-Kyu;Yoo, Byung-Tai;Kang, Gil-Jong
    • YAKHAK HOEJI
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    • v.18 no.3
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    • pp.190-196
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    • 1974
  • A new colorimetric method established for the determination of ampicillin was based on the reductions of phosphomolybdic acid by ampicillin to be resutled in a molybdenum blue which showed a mezimal absorbance at 770nm. The pH effects on a molybodenum blue solution was dependent significantly on temeprature, but the wave-length shift was not effected by the molybdenum blue.

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