• 한국버섯학회지
• /
• 제7권4호
• /
• pp.187-192
• /
• 2009

주로 고온에서 재배되는 왕송이는 온도제어기를 사용하지 않고 재배할 수 있는 잇점이 있다. 여름철 냉방시설없이 재배할 수 있는 품종을 개발하고자 국내외에서 수집된 왕송이균을 교잡하여 새로운 품종 '백련'을 육성하였다. 백련 왕송이의 균배양온도는 $25^{\circ}C$~$30^{\circ}C$이며 자실체 발생온도와 생육온도 거의 동일하다. 다만 배양완료 후 복토를 해야하기 때문에 2차 균사생장기간이 있어 초발이소요일수가 다소 늦다. 하지만 복토를 하기 때문에 주야간 온도차이에도 버섯발생이 용이하고 습도관리가 쉬워 여름철 재배가 유리하다. 버섯의 모양은 순백색에 전형적인 우산형으로 강한 다발성을 가지며 대가 긴편이다. 특히, 병재배보다는 봉지재배를 할 경우 다발성은 더욱 강해지면 자실체의 크기도 증가한다. 적정배지는 포플라 40%+미송 40%+미강 20%를 혼합한 배지이며 평균수량은 병재배시 101g이나 봉지재배에서는 더욱 증가할 것으로 사료된다.

• 한국토양비료학회지
• /
• 제45권1호
• /
• pp.9-15
• /
• 2012

This study was performed to produce basic data for silty reclaimed tidal lands and to develop techniques of environmentally-friendly utilization in agricultural system. We chose the two sites in Saemangeum reclaimed tidal lands, one (Site I) has been treated with cultivating green manure and conducting the desalinization process through submergence since April, 2007 and the other (Site II) has been under natural condition without artificial treatment. In situ and ex situ physic-chemical properties were determined and comparisons were made for soil profiles examined at these two sites in April 2009. Surface soil of Site I had lower EC and higher field saturated hydraulic conductivity than those of Site II, uncultivated land. Especially, exchangeable sodium content was lowest in Site I Ap1 layer than in other layers. This is probably due to flooding desalination and green manure cultivation. Besides, Ap1 and A2 layers of soil profile in Site I showed brighter soil color and more root observation than those of Site II. This is probably due to green manure cultivation. By the large, for high cash upland crops and intensive agricultural use of silty reclaimed tidal land, site-specific soil ripening such as flooding desalination and green manure cultivation could be useful.

• 한국세라믹학회지
• /
• 제48권4호
• /
• pp.303-306
• /
• 2011

[ $V_2O_5$ ]doped Karrooite pigments were synthesized by the solid state method to get stabilized brown pigment in oxidation and reduction atmosphere. Optimum substitution condition and limited dopant with $V_2O_5$ for Karrooite pigment was investigated. With calcination at $1250^{\circ}C{\sim}1400^{\circ}C$, compositions were designed varying $V_2O_5$ molar ratio by increasing 0.02mole to the formula $Mg_1-xTi_2-xM_{2x}O_5$(x = 0.01~0.09 mole). Synthesized pigments were analyzed by XRD, Raman spectroscopy and UV-vis. When $V_2O_5$ was doped from 0.01 to 0.05 mole, single phase of Karrooite was observed at temperature $1300^{\circ}C$ and soaking time 4h by Raman spectroscopy. However, it was found that excess $VO_2$ peak appeared with 0.07 and 0.09 mole of $V_2O_5$ doped to $MgTi_2O_5$. This result indicated that the maximum limit of solid solution is 0.05 mole $V_2O_5$. Karrooite pigments were applied as a ceramic pigment to achieve brown colors in lime magnesia glaze and lime barium graze at both of oxidation and reduction atmosphere. CIE color coordinates are $L^*$ = 40.34, $a^*$ = 9.94, $b^*$ = 21.40 in lime magnesia glaze.

• 한국조경학회지
• /
• 제35권1호
• /
• pp.88-93
• /
• 2007

Since the 1990s, the application of Kentucky bluegrass cultivars has increased in newly-constructed Korean golf courses as opposed to the previously-used zoysiagrass. However, there have been few reports studying the selection and characterization of these Kentucky bluegrass cultivars under Korean weather conditions. A total of 12 Kentucky bluegrass cultivars were tested for their adaptability in environmental stresses during summer conditions in Korea in order to select the best Kentucky bluegrass cultivars for use in Korean golf courses. This study found that two Kentucky bluegrass cultivars ('Midnight' and 'Bluestone') maintained their green throughout the summer season and had a high degree of root density, as compared to the other cultivars tested. These two cultivars also had a good rate of coverage in the early growth period. These characteristics make them suitable for application on sports fields and golf courses, which receive many divots and the frequent replacement of turfgrass sod caused by both biotic and abiotic stress. Two other cultivars, the 'Ginney' and 'Nuglade', also displayed good visual quality and high rate of coverage under summer conditions. In conclusion, the 'Midnight' and 'Bluestone' cultivars performed well in areas including the maintenance of their green color, the number of roots and the rate of coverage during the summer months. These characteristics are necessary for golf courses sports fields, which receive many divots and requirethe frequent replacement of turfgrass. further research on a range of Kentucky bluegrass cultivars, including a sensitivity test for many pathogens and recovery rates from a variety of stresses, is required in the near future.

• 한국버섯학회지
• /
• 제4권3호
• /
• pp.106-110
• /
• 2006

우수한 약리기능성을 보이는 검은비늘버섯의 병재배에 적합한 새로운 품종 진황을 교잡육종법으로 육성하였다. 진황은 포플라톱밥을 이용한 병제배와 폐면을 이용한 상자재배가 모두 가능하여 재배농가에서 생산이 용이하나, 약용 또는 기능성버섯으로서의 인지도가 낮으므로 소비 확대를 위한 약리효과의 홍보가 필요하다. 진황 검은비늘버섯의 주요특성은 다음과 같다. 가. 균사배양 최적용도는 $25^{\circ}C$ 자실체발생 및 생육온도는 $15 {\sim}18^{\circ}C$이다. 나. 자실체의 색깔은 황갈색이며 갓표면에 흰색~갈색의 인피가 덮혀 있으며 점성이 있다. 다. 병당 유효개채수가 평균 26개로 많고 대도 굵어 품질이 양호하다.

• 한국미생물·생명공학회지
• /
• 제40권4호
• /
• pp.303-309
• /
• 2012

Keratinases are of particular interest because of their action on insoluble keratins and generally on a broad range of protein substrates. Alkalophilic and neutrophilic actinomycete strains isolated from different soil samples, rich in keratinaceous substances were screened for keratinolytic activity. An alkalophilic isolate, TBG-S13A5, was found to possess good keratinolytic activity and was able to utilize feather as the sole carbon and nitrogen source. TBG-S13A5 exhibited an off-white aerial mass color with a rectus-flexibilis type of spore chain. The morphological, microscopical and biochemical characters were comparable with that of Streptomyces albidoflavus. Fatty acid methyl ester profiling (FAME) and 16S rDNA sequence analysis confirmed its identity as a strain of S. albidoflavus. Under submerged fermentation conditions, maximum protease production was recorded on the $5^{th}$ day of incubation at $30^{\circ}C$, using basal broth of pH 9.0 with 0.25% (w/v) white chicken feather. This strain could affect feather degradation when the initial pH was 8 and above and maximum protease production was recorded when the initial pH was around 10.5. The effectiveness of the crude enzyme in destaining and leather dehairing were also demonstrated.

• 한국수산과학회지
• /
• 제46권1호
• /
• pp.27-36
• /
• 2013

This study was conducted to develop and characterize sea squirt Halocynthia roretzi sikhae. According to the results for pH, total acidity, lactic acid bacteria, amino nitrogen and sensory evaluation of sea squirt sikhae during fermentation for 6 days at $15^{\circ}C$, the optimum fermentation periods were 4 days for sourness-disliking customers and 5 days for sour-disliking customers. No differences in the proximate compositions of sea squirt sikhaes fermented for 4 days (4D) and for 5 days (5D) were found. There was a difference in the eproximate compositions of commercial seasoned sea squirts, 4D and 5D, sea squirt sikhaes. The results of salinity, total acidity, amino nitrogen and sensory evaluation of two kinds of sikhae suggest that the taste was stronger for 5D than for 4D, both of which were superior to commercial seasoned sea squirts. There was, however, no difference in color of 4D, 5D and commercial seasoned sea squirts. The results of E. coli analyses suggest that sea squirt sikhae is a safe food in terms of sanitation.

• Journal of Microbiology and Biotechnology
• /
• 제24권10호
• /
• pp.1354-1367
• /
• 2014

In the present work, we describe a simple, cheap, and unexplored method for "green" synthesis of silver nanoparticles using cell extracts of the cyanobacterium Anabaena doliolum. An attempt was also made to test the antimicrobial and antitumor activities of the synthesized nanoparticles. Analytical techniques, namely UV-vis spectroscopy, X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), and TEM-selected area electron diffraction, were used to elucidate the formation and characterization of silver-cyanobacterial nanoparticles (Ag-CNPs). Results showed that the original color of the cell extract changed from reddish blue to dark brown after addition of silver nitrate solution (1 mM) within 1 h, suggesting the synthesis of Ag-CNPs. That the formation Ag-CNPs indeed occurred was also evident from the spectroscopic analysis of the reaction mixture, wherein a prominent peak at 420 nm was noted. TEM images revealed well-dispersed, spherical Ag-CNPs with a particle size in the range of 10-50 nm. The X-ray diffraction spectrum suggested a crystalline nature of the Ag-CNPs. FTIR analysis indicated the utilization of a hydroxyl (-OH) group in the formation of Ag-CNPs. Ag-CNPs exhibited strong antibacterial activity against three multidrug-resistant bacteria. Additionally, Ag-CNPs strongly affected the survival of Dalton's lymphoma and human carcinoma colo205 cells at a very low concentration. The Ag-CNPs-induced loss of survival of both cell types may be due to the induction of reactive oxygen species generation and DNA fragmentation, resulting in apoptosis. Properties exhibited by the Ag-CNP suggest that it may be used as a potential antibacterial and antitumor agent.

• Mycobiology
• /
• 제32권2호
• /
• pp.88-94
• /
• 2004

Protoplasts of the wild type strain of Pleurotus osteatus were mutagenized with UV light, and 3,000 colonies were examined for abnormal mycelial and fruiting phenotypes. Forty one strains displayed variant phenotypes in mycelia and fruiting processes. The variant phenotypes were classified into 6 groups: (1) auxotrophic strains, which are incapable of growing on minimal media and can only grow when provided with their specific requirements; (2) abnormal vegetative strains, which grow very slowly on minimal and complete media; (3) primordiumless strains, which fail to develop to the formation of primordia; (4) maturationless strains, which form primordia, but do not form mature fruiting bodies; (5) specifically colored strains, which have Specific bluish grey or bluish white pileus; (6) poorly spored strains, which fail to produce basidiospore or which produce few spores. These variant strains may be useful in genetic breeding programs and for the studies of fungal development and genetics.

• 한국결정성장학회지
• /
• 제19권4호
• /
• pp.165-171
• /
• 2009

기존의 합성방법에 사용되는 백금 도가니의 단점을 보완할 수 있는 알루미나 도가니를 사용하여 천연 및 기존의 합성루비와 유사한 적색의 투명한 결정을 성장시켰다. $PbO-B_2O_3$ 가 흔합된 플럭스를 사용하여 $915{\sim}1350^{\circ}C$의 온도에서 약 240시간 실험 후 최대 9.02 ${\times}$ 6.36 mm 투명한 적색의 결정이 성장되었다. 이 결정을 보석학적 기초검사를 통하여 굴절률, 내포물, 비중 등의 특성과 FT-IR, UV-VIS spectrometer 및 XRD 를 사용하여 기존의 합성루비 및 천연루비의 특성과 비교분석하였다.