• 제목/요약/키워드: Color and fluorescence

검색결과 169건 처리시간 0.038초

Comparison of Clinical Characteristics of Fluorescence in Quantitative Light-Induced Fluorescence Images according to the Maturation Level of Dental Plaque

  • Jung, Eun-Ha;Oh, Hye-Young
    • 치위생과학회지
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    • 제21권4호
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    • pp.219-226
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    • 2021
  • Background: Proper detection and management of dental plaque are essential for individual oral health. We aimed to evaluate the maturation level of dental plaque using a two-tone disclosing agent and to compare it with the fluorescence of dental plaque on the quantitative light-induced fluorescence (QLF) image to obtain primary data for the development of a new dental plaque scoring system. Methods: Twenty-eight subjects who consented to participate after understanding the purpose of the study were screened. The images of the anterior teeth were obtained using the QLF device. Subsequently, dental plaque was stained with a two-tone disclosing solution and a photograph was obtained with a digital single-lens reflex (DSLR) camera. The staining scores were assigned as follows: 0 for no staining, 1 for pink staining, and 2 for blue staining. The marked points on the DSLR images were selected for RGB color analysis. The relationship between dental plaque maturation and the red/green (R/G) ratio was evaluated using Spearman's rank correlation. Additionally, different red fluorescence values according to dental plaque accumulation were assessed using one-way analysis of variance followed by Scheffe's post-hoc test to identify statistically significant differences between the groups. Results: A comparison of the intensity of red fluorescence according to the maturation of the two-tone stained dental plaque confirmed that R/G ratio was higher in the QLF images with dental plaque maturation (p<0.001). Correlation analysis between the stained dental plaque and the red fluorescence intensity in the QLF image confirmed an excellent positive correlation (p<0.001). Conclusion: A new plaque scoring system can be developed based on the results of the present study. In addition, these study results may also help in dental plaque management in the clinical setting.

Indocyanine green 형광조영제의 형광발현을 위한 농도 범위 분석 (Concentration Range Analysis for Fluorescence Expression of Indocyanine Green)

  • 김용재;이다애;윤기철;김광기
    • 한국멀티미디어학회논문지
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    • 제22권12호
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    • pp.1339-1346
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    • 2019
  • In the characteristic of the brain malignant, the blood vessels and tumors have the same color and shape, and the boundary distinction is not clear, Therefore, it is difficult to observe the naked eye. Because of the high invasiveness, the risk of recurrence is high. Therefore, complete resection of the tumor is essential. The method for distinguishing the boundary between blood vessels and tumors is a fluorescence contrast method using indocyanine green (ICG), a fluorescence contrast agent. In ICG, the concentration range analysis is very important because the fluorescence expression state varies depending on the concentration. However, since the analysis result of the fluorescence expression condition is insufficient according to the current concentration, this paper proposes by analyzing the initial protocol of the concentration range. 780 nm infrared light was irradiated to the ICG sample to observe the fluorescence expression through a near infrared (NIR) camera. The wavelength is measured by using a spectrum instrument (ocean view) to observe the fluorescence expression wavelength of 811nm. As a result of analyzing the mol concentration according to each sample, the fluorescence expression range was found to be 16.15-0.16M and the optimum fluorescence concentration on the brightest part was found to be 3.23-0.81M.

열 활성 지연 형광(TADF) 재료의 특허 분석 (The Patent Analysis of Thermally Activated Delayed Fluorescence Materials)

  • 조대성;성민재;김민호;최승철
    • 반도체디스플레이기술학회지
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    • 제18권1호
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    • pp.105-111
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    • 2019
  • The TADF (Thermally Activated Delayed Fluorescence)-based OLED patents were analyzed and 4410 of patents were selected at the first step. And 975 patents were screened at second step. Finally, 39 key patents were selected. Patent qualitative analysis was performed in these patents to find which of the four property (lifetime, efficiency, color purity, driving voltage) of TADF was improved. Also, the variation of the hosts and dopants in patented TADF material were surveyed and their combination was analyzed. According to the analysis of the variation and the combination, some of TADF compounds were used as an assistant dopant to transfer energy. In addition, it tended to transfer energy by forming exciplex that shows TADF characteristics. These were similar to the mechanism of the introduced hyper fluorescence and could solve the inherent TADF problems. Finally, patent citation network was illustrated to visualize the patent citations and citations relationship of the major applicants in the current TADF-based OLED technology. The leading patent applicant organization was revealed as Idemitsu Kosan, Semiconductor Energy Laboratory, UDC, Princeton University, Merck and Nippon Steel & Sumikin Chemical, which had lots of reference patents 559, 524, 477, 310, 258, and 167, respectively.

고강도/고분자량 폴리에틸렌 섬유의 적색 초소수성 형광염료 염색 (Dyeing of High Strength and High Molecular Weight Polyethylene Fiber Using Super Hydrophobic Red Fluorescence Dyes)

  • 김태건;이준헌;박지훈;김태경
    • 한국염색가공학회지
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    • 제30권4호
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    • pp.237-244
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    • 2018
  • Three super hydrophobic red fluorescence dyes were selected to dye high molecular weight polyethylene fiber. Their absorbance and emission spectra were obtained and Stokes' shift was measured. Fluorescence emission strength of the dyes on the fiber was investigated and therefore Fluoro Red 3 was determined as the best one among those three dyes in this experiment. Dyeing properties and fluorescence intensities were investigated using the Fluoro Red 3 on high molecular weight polyethylene fiber at various dyeing conditions. The optimum concentration of a dispersing agent was appeared at 10wt% in aqueous solution. The best dyeing was obtained at $125^{\circ}C$ for 1 hour. The color fastnesses to the washing and rubbing were as high as ratings 4~5, however, the fastness to light was exhibited ratings 2~3.

Fluorescence Quenching Causes Systematic Dye Bias in Microarray Experiments Using Cyanine Dye

  • Jeon, Ho-Sang;Choi, Sang-Dun
    • Genomics & Informatics
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    • 제5권3호
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    • pp.113-117
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    • 2007
  • The development of microarray technology has facilitated the understanding of gene expression profiles. Despite its convenience, the cause of dye-bias that confounds data interpretation in dual-color DNA microarray experiments is not well known. In order to economize time and money, it is necessary to identify the cause of dye bias, since designing dye-swaps to reduce the dye-specific bias tends to be very expensive. Hence, we sought to determine the reliable cause of systematic dye bias after treating murine macrophage RAW 264.7 cells with 2-keto-3-deoxyoctonate (KDO), interferon-beta $(IFN-{\beta})$, and 8-bromoadenosine (8-BR). To find the cause of systematic dye bias from the point of view of fluorescence quenching, we examined the correlation between systematic dye bias and the proportion of each nucleotide in mRNA and oligonucleotide probe sequence. Cy3-dye bias was highly correlated with the proportion of adenines. Our results support the fact that systematic dye bias is affected by fluorescence quenching of each feature. In addition, we also found that the strength of fluorescence quenching is based on not only dye-dye interactions but also dye-nucleotide interactions as well.

A Cyan Fluorescent Protein Gene (cfp)-Transgenic Marine Medaka Oryzias dancena with Potential Ornamental Applications

  • Vu, Nguyen Thanh;Cho, Young Sun;Lee, Sang Yoon;Kim, Dong Soo;Nam, Yoon Kwon
    • Fisheries and Aquatic Sciences
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    • 제17권4호
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    • pp.479-486
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    • 2014
  • To evaluate their potential utility as an ornamental organism, novel transgenic marine medaka Oryzias dancena strains with a highly vivid fluorescent phenotype were established through transgenesis of a cyan fluorescent protein gene (cfp) driven by the endogenous fast skeletal myosin light chain 2 gene (mlc2f) promoter. The transgenic marine medaka strains possessed multiple copies of transgene integrants and passed their fluorescent transgenes successfully to subsequent generations. Transgenic expression in skeletal muscles at both the mRNA and phenotypic levels was, overall, dependent upon transgene copy numbers. In the external phenotype, an authentic fluorescent color was dominant in the skeletal muscles of the transgenic fish and clearly visible to the unaided eye. The phenotypic fluorescent color presented differentially in response to different light-irradiation sources; the transgenics displayed a yellow-green color under normal daylight or white room light conditions, a strong green-glowing fluorescence under ultraviolet light, and a cyan-like fluorescence under blue light from a light-emitting diode.

자색고구마로부터 붉은 색소 농축물의 추출 및 그 색소 분말의 제조 특성 (Extraction of Natural Red Color Pigment Concentrate and Manufacturing Characteristics of Pigment Powder from Purple-Fleshed Sweet Potato)

  • 구본순;송대식
    • 한국식품영양학회지
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    • 제23권1호
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    • pp.23-29
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    • 2010
  • Pigment concentrates with violet-red color and sweet taste were obtained from purple-fleshed sweet potato(PFSP) using ethyl alcohol and water. Extract from general potato(GP) were used as a control. The relative stability of PFSP pigment concentrate(PFSPPC) in a storage test over 15 days was confirmed in the order of dark > fluorescence > sun-light irradiation. The relative stability of GP pigment concentrate(GPPC) in a storage test over 15 days was confirmed in the order of sun-light > fluorescence > dark storage. The RRP of PFSPPC was higher than that of GPPC, but the color strength of GPPC was 1/2 that of PFSPPC. Treatment of PFSPPC with aluminum potassium sulfate(0.2~0.3%, w/w) best improved its stability. The improved RRPs of PFSPPC were 45.16~47.31% in sun light irradiation, 55.91~60.22% in fluorescence irradiation, and 76.34~75.97% in dark storage conditions. In substituting aluminum potassium sulfate for chitosan, an amount of 0.2~0.3%(w/w) was suitable, giving similar results in improving pigment stability for all concentrates tested. Also, freeze-dried PFSPPC powder was manufactured as a substitute for dextrin, and also as a substitute for chitosan to the extent of 0.25%(w/w). The results of storage stabilite for freeze-dried PFSPPC and GPPC powder over 15 days, irradiation were, PRRs of 74.47~89.36% and 61.54~76.92%, respectively. The stability improving effect of freeze dried PFSPPC powder was confirmed by the results of storage experiments at various conditions. The use of freeze-dried PFSPPC powder was therefore confirmed to be an effective treatment for general foods.

Solution-Processible Blue-Light-Emitting Polymers Based on Alkoxy-Substituted Poly(spirobifluorene)

  • Lee, Jeong-Ik;Chu, Hye-Yong;Oh, Ji-Young;Do, Lee-Mi;Lee, Hyo-Young;Zyung, Tae-Hyoung;Lee, Jae-Min;Shim, Hong-Ku
    • ETRI Journal
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    • 제27권2호
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    • pp.181-187
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    • 2005
  • Alkoxy-substituted poly(spirobifluorene)s and their copolymers with a triphenylamine derivative have been synthesized by Ni(0)-mediated polymerization. The polymers were well soluble in common organic solvents. Pure blue-light emissions without the long wavelength emission of poly(fluorene)s have been observed in the fluorescence spectra of polymer thin films. The light emitting diodes with a device configuration of ITO/PEDT:PSS(30 nm)/polymer(60 nm)/LiF(1 nm)/Al(100 nm) have been fabricated. The electroluminescence spectra showed the blue emissions without the long wavelength emission as observed in the fluorescence spectra. The relatively poor electroluminescence quantum yield of the homopolymer (0.017% @ 20 $mA/cm^{2}$) with color coordinates of (0.16, 0.07) has been improved by the introduction of triphenylamine moiety, and the copolymer with derivative exhibited an electroluminescence quantum yield of 0.15 % at 20 $mA/cm^{2}$ with color coordinates of (0.16, 0.08). Moreover, the introduction of polar side chains to the spirobifluorene moiety enhanced the device performance and led to the quantum yields of 0.6 to 0.7 % at 20 $mA/cm^{2}$, although there was some expense of color purities.

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Transient Protection of Intramolecular Hydrogen Bonding: A Simple but Elegant Approach for Functional Imaging

  • Kim, Jong-Man;Min, Sung-Jun;Park, Bum-Jun;Lee, Jae-Hyung;Ahn, Kwang-Duk
    • Macromolecular Research
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    • 제12권5호
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    • pp.493-500
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    • 2004
  • We have developed a novel method for patterning functional images in thin polymer films. The key materials we utilized for the imaging were dihydroxyanthraquinones protected with acid-labile tert-butoxycarbonyl (t-Boc) blocking groups. Among the tested compounds, 1,4-dihydroxyanthraquinone (quinizarin; 1) underwent the most drastic change in terms of its color and fluorescence upon protection. We prepared the t-Boc-protected quinizarin and polymers bearing the protected quinizarins as pendent groups. To investigate the possibility of a single-component imaging system, we synthesized a styrenic monomer 14 incorporating protected quinizarin and a maleimide derivative 15 bearing a photoacid generating group and subjected them to polymerization. Selective removal of the protecting groups of the quinizarin moieties in the exposed area using photolithographic techniques allowed regeneration of quinizarin and patterned fluorescence images in the polymer films.

상동산 SCHEELITE의 특성연구 <특히 Powellite 진부 구명을 위하여>

  • 황재운
    • 기술사
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    • 제3권10호
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    • pp.3-6
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    • 1970
  • The scheelite occurred in Sang Dong mine are disseminated with a fine grain, and shelved pale blue or white color under the fluorescence. But among them, We can often observe the yellow fluorescent color mineral grains, which were called the powellite from the past time without any detail mineral study. I studied the characteristics of Sang-Dong scheelite and the so called powellite. In the result, the so called powellite is proved to be the molybdenum bearing scheelite in the view of specific gravity, index of refraction, chemical composition, other microscopic properties, and dressing. Such a scheelite is contained usually Zor 3% Mo, and oftenly 4 or 6% Mo, and fluorescent colors are pale yellow, leman yellow and chrome yellow.

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