• Korean Journal of Microbiology
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• v.23 no.4
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• pp.248-251
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• 1985

An imperfect fungus recognized as a dermatophyte was isolated from soil samples and the macroscopic, microscopic and physiological characteristics were examined. From the comparisons of this isolate with the known species, the strain belonged to the genus Microsporum. She showed mant different characteristics from the related species, including colony color and shape, or number in a cell of macrocondium. This isolate was considered to be a novel species in the genus Microsporum. Therefore the name was given to her as Microsporum nigricans Yeeh sp. nov. because of dark blackish color from the macroscopic observation.

• Mycobiology
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• v.39 no.2
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• pp.85-91
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• 2011

We investigated the effect of nutritional and environmental factors on Ophiocordyceps longissima mycelial growth. The longest colony diameter was observed on Schizophyllum (mushroom) genetics complete medium plus yeast extract, Schizophyllum (mushroom) genetics minimal medium, and Sabouraud dextrose agar (SDA); however, malt-extract yeast-extract agar, SDA plus yeast extract, yeast-extract malt-extract peptone dextrose agar, SDA, oatmeal agar, and potato dextrose agar showed higher mycelia density. A temperature of $25^{\circ}C$ was optimum and 7.0 was the optimum pH for mycelial growth. Colony diameter was similar under light and dark conditions. Maltose and yeast extract showed the highest mycelial growth among carbon and nitrogen sources respectively. The effect of mineral salts was less obvious; however, $K_3PO_4$ showed slightly better growth than that of the other mineral salts tested. Among all nutrition sources tested, complex organic nitrogen sources such as yeast extract, peptone, and tryptone were best for mycelial growth of O. longissima. Ophiocordyceps longissima composite medium, formulated by adding maltose (2% w/v), yeast extract (1% w/v), and $K_3PO_4$ (0.05% w/v) resulted in slightly longer colony diameter. In vitro mycelial O. longissima growth was sustainable and the production of fruiting bodies could be used for commercial purposes in the future.

• Journal of Embryo Transfer
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• v.7 no.1
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• pp.49-55
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• 1992

Animal experiments need numerous kinds of animal which are suitable for every research. About 300 mouse strains are developed up to the present, but they do not give satisfaction to every researchers. So we must build up the methods of breading animals which are newly developed and of maintenance of characteristics which were developed before. To maintain experimental animal is not only proceeding the generation but also increasing the animal populations, it needs geneticai control. Genetic factors which influence to reproduction are very important to maintain colony. These factors include lethal gene, chromosomal abberation, sterility gene, etc.. With the recent development of transgenic technology, scientists now can deliberately creat numerous specific animal models. To know how to manage the colony which has genetic defect on reproduction and transgenic mice is one of the key to study in vitro fertilization.

• Mycobiology
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• v.38 no.2
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• pp.118-121
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• 2010

Single ascospore isolates of Cordyceps bassiana were observed for their colony pigmentation on Sabouraud Dextrose agar plus Yeast Extract (SDAY) plates and were inoculated in a brown rice medium for production of fruiting bodies. Colony pigmentation did not show any relationship with perithecial stromata formation. The isolates were also grown on opposite sides of SDAY agar plates and were observed for vegetative compatibility. Neither vegetative compatibility nor perithecial stromata could be found to be related to each other. It was concluded that fertile fruiting body production was independent of colony pigmentation and vegetative compatibility. Synnemata formation was found to be more common than perithecial stromata formation. This might be due to its highly conidiogenous anamorphic stage, i.e., Beauveria bassiana.

• Journal of the Korean Society for Precision Engineering
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• v.29 no.4
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• pp.373-379
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• 2012

Disruptions in manufacturing systems caused by system changes and disturbances such as the tool wear, machine breakdown, malfunction of transporter, and so on, reduce the productivity and the increase of downtime and manufacturing cost. In order to cope with these challenges, a new method to build an intelligent manufacturing system with biological principles, namely an ant colony inspired manufacturing system, is presented. In the developed system, the manufacturing system is considered as a swarm of cognitive agents where work-pieces, machines and transporters are controlled by the corresponding cognitive agent. The system reacts to disturbances autonomously based on the algorithm of each autonomous entity or the cooperation with them. To develop the ant colony inspired manufacturing system, the disturbances happened in the machining shop of a transmission case were analyzed to classify them and to find out the corresponding management methods. The system architecture with the autonomous characteristics was generated with the cognitive agent and the ant colony technology. A test bed was implemented to prove the functionality of the developed system.

• Journal of architectural history
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• v.11 no.2 s.30
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• pp.7-22
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• 2002

Architectural style is said to be product reflecting political, social and cultural condition. Especially, in colony, architectural style is strongly related to political condition or policy. After colonization in 1910, public buildings with western historical architectural style in Japanese version were widely built by Japanese colonial government in Korea. And in the late 1920s, modernism style in architecture became dominant in Korea as like other countries. In this situation, curious buildings in strange architectural styles came out. One example is railroad station buildings with traditional Korean architectural style and timber house station having a steep roof which is widely used in North Europe such as Alps area with good sights and mountains. Generally, the colonizer says that colonization is the only way to save the colony at crisis defined by colonizer and they insist that they can help the colony modernize. To justify colonization, the colonizer attributes the colonization to the characteristics of the nation and stagnation of the traditional culture etc.. Accordingly, the colonizer tries to depreciate colony's traditional value and culture. In case of colony which has similar cultural background historically (in this case, economical exploitation is less important than other Asian colony by European power), this depreciation of traditional value and culture in Korea was done more strongly than others. At this time, we should understand special relation between Korea and Japan historically. Even though, colony's locality is adopted by the colonizer in public fields, which is based on political purpose or exotic taste etc.. In early days of Japanese ruling period, Japan never use the Korean traditional facts in public. Therefore there is no use of Korean traditional architectural style in public field. In late 1920s, some railroad station buildings were constructed in new styles without precedence in modern Korea. One is railroad station buildings in Korean traditional architectural style, the other is railroad station buildings in timber house stations having a steep roof which is different form western historical architectural style. It was mystery that Japan had constructed railroad stations in Korean traditional style which Japan had tried to destroy together with Western style railroad station buildings. This paper is made to solve the mystery why the colonizer(Japan) constructed entirely different types of railroad stations at the same time in the late 1920s and 1930s. The key point to solve this mystery is tourism. In this paper, to solve this mystery, I try to use terminology' 'Colonial Tourism' in architecture why colonial power had constructed railroad stations with colony's traditional architectural style and Western style having a steep roof which can be seen north European region.

• Journal of Food Hygiene and Safety
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• v.14 no.3
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• pp.227-232
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• 1999

A total of 136 coliform bacteria isolated from spring water (112 strains) and ground water (24 strains), submitted to Seoul Health and Environmental Research Institute from June to July in 1997, were characterized biochemically and microbiologically. Colonical characteristics of each isolate were also noted, including color and texture on EMB agar. Among the 136 isolates, 50.7% were greenish metallic sheen color, 44.2% were pink and 5.1% were violet. The sixty four percent were smooth, 34.6% were mucoid and 0.7%. were rough. Twenty three bacterial species were identified by IMViC and API 20E test. Among the 136 coliform bacteria known to species, 39 isolates (28.6%) were Escherichia spp., 32 isolates (23.5%) were Klebsiella ssp., 30 isolates (22.1%) were Enterobacter spp., 19 isolates (14.0%) were Serratia spp., 6 isolates (4.4%) were Citrobacter spp., 4 isolates (3.0%) were Kluyvera spp. and 7 isolates (5.1%) were other bacterial species. Strains, which were gas-positive in lactose broth but gas-negative in Kligler Iron Agar were Ent. intermedium, Ser. liquefaciens, Ser. marcescenes and Salmonella arizoae. Strains, which were H2S production were also Kleb. pneumoniae, Kleb. oxytoca, Kleb. ornithinolytica, Ent. sahazahii, Ent. cloacae, Ser. liquefaciens, Ser. fica ria, Cit. freundii and Sal. arizoae. In the present study, most of coliform isolated from spring and ground water were E. coli, Klebsiella spp. and Enterobacter spp. Since coliform with pink colony in EMB agar was isolated as frequent as coliform with greenish metallic sheen colony, coliform with pink colony should be considered as important colony. Our results suggested that new coliform strains may be emerging on the basis of biochemical and microbiological testes.

• International Journal of Industrial Entomology and Biomaterials
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• v.24 no.1
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• pp.29-35
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• 2012

Bumblebees are widely used to pollinate various crops, especially tomato, in greenhouses and fields. Here, we investigated whether different sugar solutions have any effects on the oviposition and colony development of Bombus terrestris queens. The types of sugar solution used were a white sugar solution, brown sugar solution, dark brown sugar solution, and prepared sugar solution; all solution contained with a 40% sugar concentration and 0.3% sorbic acid. Among these sugar solutions, B. terrestris queens reared on the white sugar solution exhibited the best results; the rates of oviposition, colony foundation and progeny-queen production were $75.0{\pm}11.0$, $33.0{\pm}9.6$ and $21.7{\pm}7.3$, respectively, which corresponded to 1.1-33.0-fold increases over the results of other sugar solutions. Queens reared on the prepared sugar solution did not found any colonies. The death rate within one month was lowest for the queens reared on the white sugar solution at $26.1{\pm}10.9%$, which was 1.2-2.0-fold lower than the rate for the other sugar solutions. Furthermore, the numbers of progeny produced by queens reared on the white sugar solution, $131.4{\pm}38.8$ workers and $51.0{\pm}40.6$ queens, were also higher, corresponding to 1.1-1.2-fold increases compared to queens raised on the other sugar solutions. Therefore, the white sugar solution was the favorable sugar solution for the egg-laying and colony developmental characteristics of B. terrestris queens.

• Journal of Apiculture
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• v.33 no.4
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• pp.261-268
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• 2018

Queen honey bees (Apis cerana) can be reared artificially on demand by the use of grafting technique. The technique consists of grafting young worker larvae into queen cell cups and raising in a queenless strong colony. As the age of grafted larvae for queen rearing exhibited several aspects related to quality and reproductive potential of queen, this study were conducted to investigate the influence of age of grafted larva on morphological characteristics and lifespan of queen, and the growth of colony she headed. Our results demonstrated that queens reared from young worker larvae (i.e., less than 1-day old larvae) were significantly larger in size (i.e., body weight and thorax width) than that of queens reared from 2-day old worker larvae. Moreover, queens reared from younger worker larvae initiated egg-laying earlier, stored more spermatozoa in spermatheca and had a longer lifespan compared to queens raised from older worker larvae. We also found a significant positive effect of queen grafting age on the production of worker and drone brood, adult worker population in colonies headed by queens reared from younger larvae. These findings suggested that rearing queens from brood grafted at the earliest possible age could increase the reproductive potential of queen as well as fitness of colony she head.

• The Plant Pathology Journal
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• v.27 no.2
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• pp.128-137
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• 2011

Fifty two Macrophomina phaseolina isolates were recovered from 24 host plant species through the 14 Iranian provinces. All isolates were confirmed to species using species-specific primers. The colony characteristics of each isolate were recorded, including chlorate phenotype, relative growth rate at $30^{\circ}C$ and $37^{\circ}C$, average size of microsclerotia, and time to microsclerotia formation. The feathery colony phenotype was the most common (63.7%) on the chlorate selective medium and represented the chlorate sensitive phenotype of the Iranian Macrophomina phaseolina population. Meantime, inter simple sequence repeats (ISSR) Markers were used to assess the genetic diversity of the fungus. Unweighted pair-group method using arithmetic means (UPGMA) clustering of data showed that isolates did not clearly differentiate to the specific group according to the host or geographical origins, however, usually the isolates from the same host or the same geographic origin tend to group nearly. Our results did not show a correlation between the genetic diversity based on the ISSR and phenotypic characteristics. Similar to the M. phaseolina populations in the other countries, the Iranian isolates were highly diverse based on the phenotypic and the genotypic characteristics investigated and needs more studies using neutral molecular tools to get a deeper insight into this complex species.