• Title/Summary/Keyword: Colony characteristics

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Two New Records of Penicillium Associated with Blue Moldy Bulbs of Lily in Korea

  • Kim, Won-Ki;Park, Myung-Soo;Hahm, Soo-Sang;Yu, Seung-Hun
    • Mycobiology
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    • v.34 no.4
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    • pp.176-179
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    • 2006
  • Two new records of Penicillium from blue moldy bulbs of lily are reported in Korea. The Korean isolates of P. albocoremium (Frisvad) Frisvad and P. tulipae Overy and Frisvad were phylogenetically identical to the reference species based on DNA sequence of the ${\beta}-tubulin$ gene. P. albocoremium and P. tulipae are described and illustrated.

Identification of the Fungal Pathogen that Causes Strawberry Anthracnose in Bangladesh and Evaluation of In Vitro Fungicide Activity

  • Akhter, Shamim;Alam, Shahidul;Islam, Shafiqul;Lee, Min-Woong
    • Mycobiology
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    • v.37 no.2
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    • pp.77-81
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    • 2009
  • This study was conducted to identify the Colletotrichum species causing anthracnose disease of strawberry in Balgladesh and to evaluate in vitro activity of commercial fungicides it. Based on morphological and cultural characteristics, all 22 isolates were identified as Colletotrichum gloeosporioides. They developed white or glittery colonies with grey to dark grey reverse colony colors and they produced cylindrical conidia. The efficacy of five commercial fungicides, Bavistin DF, Dithane M-45, Sulcox 50 WP, Corzim 50 WP and Rovral 50 WP, were tested against the fungus. Bavistin inhibited radial growth completely and was followed in efficacy by Dithane M-45. In Bavistin DF treated media, the fungus did not produce conidia. The percent inhibition of radial growth of the fungus was increased with the increasing concentrations of fungicide.

The first record of nine bacterial species belonging to the phylum Proteobacteria in Korea

  • Kim, Dong-Uk;Kang, Myung-Suk;Kim, Ju-Young;Kim, Myung Kyum
    • Journal of Species Research
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    • v.6 no.3
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    • pp.214-223
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    • 2017
  • As part of a larger study with the aim to discover indigenous prokaryotic species in Korea, nine bacterial strains were isolated and assigned to the phylum Proteobacteria in 2016. High 16S rRNA gene sequence similarity (>98.5%) and formation of a robust phylogenetic clades with known species indicated that each strain belongs to an independent and predefined bacterial species. This is the first report of these nine species in Korea: two strains of the Methylobacterium, two strains of the Microvirga, one strain of the Pantoea, and four strains of the Psychrobacter, all within the Proteobacteria. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.

A Study on Characteristics of Water Pollution in Woopo-Mokpo Wetlands (우포·목포늪 수질오염특성 연구)

  • Lee, Tae-Young;Yoon, Sung-Yoon
    • Journal of Wetlands Research
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    • v.1 no.1
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    • pp.1-5
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    • 1999
  • We could know that the Woopo and Mokpo wetlands, which have the typical colony structure in the agricultural area of korea, had a characteristic which the pollution sources of water are scattered widely and it is caused by sewage, livestock wastewater and non point sources. As the result of an examination of water, the concentration of T-N was measured highly within five grade in the lake standard. If we build natural domestic treatment system in Taehap-myon, lbang-myon and Yuo-myon, which are located at the upper stream of wetlands, we can remove SS, BOD, COD and T-N largely.

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A report of 10 unrecorded bacterial species of Korea, isolated from agricultural soil in 2022

  • Oung Bin Lim;Ji Soo Lee;Hyosun Lee;Ki Eun Lee;In Tae Cha;Won Jae Chi;Dong-Uk Kim
    • Korean Journal of Environmental Biology
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    • v.41 no.3
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    • pp.215-222
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    • 2023
  • In 2022, research for native prokaryotic species in Korea reported 10 unrecorded bacterial strains affiliated to phyla Actinomycetota, Bacillota, and Pseudomonadota. The strains formed monophyletic clades with the most closely related species (with ≥98.7% sequence similarity) in the 16S rRNA gene sequencing. Among them, four species of the phylum Actinomycetota, two species of the phylum Bacillota, and four species of the phylum Pseudomonadota have not been reported in Korea, suggesting unrecorded species in Korea. Information on strains such as Gram staining reaction, colony and cell morphology, biochemical characteristics, and isolation sources were provided in the species description.

A report of six unrecorded bacterial species isolated from soil samples in Korea

  • Da Som Kim;Mi Jin Jeon;Won-Jae Chi
    • Journal of Species Research
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    • v.13 no.1
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    • pp.61-66
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    • 2024
  • During an investigation of unrecorded prokaryotic species in Korea, six unrecorded bacterial strains were isolated from soil samples collected from Uljin-gun. Based on a similarity search using the 16S rRNA gene sequence of the isolated strains and the construction of the neighbor-joining phylogenetic tree, five strains were identified to the genus Pseudomonas of the family Pseudomonadaceae, while one strain was identified as a species belonging to the genus Paenibacillus of the family Paenibacillaceae. The details of these unreported species, including gram staining reaction, colony and cell morphology, basic biochemical characteristics, strain ID, and isolation source, are described in the description of the strains.

A STUDY ON THE MYCELIA CUL TURE AND GENETIC CHARACTERISTICS IN Lepista nuda (민자주방망이버섯(Lepista nuda)의 균사배양 및 유전적 특성에 관한 연구)

  • 김종봉;황성구
    • Journal of Life Science
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    • v.11 no.5
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    • pp.496-501
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    • 2001
  • This study was carried out to investigate the cultural characteristics and the genetic characteristics in Lepists nuda. The mycelia cultural characteristics which include the specificity for pH, carbon source, nitrogen source and medium were studied using petridish culture and liquid media culture respectively. The genetic characteristics were also investigated using random amplified polymorphic DNA (RAPD) analysis. To find out the mycelial growth rate by various medium, mycelia was cultured for 25 days in PDA, YM, MEA, GPB and Yamanaka. The Yamanaka was superior to the other media in supporting the mycelial growth. pH6 produced the best result in the test of an optimal pH. In experiment for optimal carbon source, starch showa 46.8$\pm$1.7mm of diameter of mycelial colony as the best and for nitrogen source, yeast extract showed a good effect as well. In investigation for genetic characteristics, Lepists nuda was amplified by 7 primers among 10 primers and Pleurotus ostreatus was also amplified by 7 primers. From the RAPD analysis between species, the band patterns were different.

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Geochemical Characteristics and Assesment of Nitrate Nitrogen in Groundwater in Yanggu-Gun, Gangwon-Do in Korea

  • Choi, Won Gyu
    • Journal of Soil and Groundwater Environment
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    • v.24 no.6
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    • pp.26-32
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    • 2019
  • An analysis of groundwater quality is significant for monitoring and managing water contamination and groundwater system. For the purpose of those, the geochemical characteristics of groundwater were studied over the concern for water quality, water type and origin of nitrate nitrogen. Total colony counts were detected in 11 out of 20 samples, and the average value was 31.73 CFU/ml. Range and average of NO3-N concentrations were 0.9~24.0 mg/L and 8.3 mg/L. All groundwater types were found to be Ca2+-HCO3-. The range and average of NO3-N were 0.2~17.4 mg/L and 8.7 mg/L, and those of δ15N were 1.7~8.9‰, and 5.0‰. Careful consideration is required for evaluating the origin of nitrogen when NO3-N concentration is low. In general, noticeable difference between rockbed and alluvial water was not found. The ranges of nitrate origins by chemical fertilizer, livestock manure and domestic sewage, and natural soil were 29.6~76.4%, 14.2~58.9% and 2.6~7.0%, and the average values of those were 57.4%, 37.4%, and 5.3%, respectively. Origin of nitrate was affected by more chemical fertilizer than the other parameters. Rockbed water was more affected by chemical fertilizer than alluvial water.

Two new species of Trichoderma isolated from commercially grown oyster mushroom, Pleurotus ostreatus (oral)

  • Park, Myung-Soo;Seo, Geon-Sik;Bae, Kyung-Sook;Yu, Seung-Hun
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.127.1-127
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    • 2003
  • We describe two new Trichoderma species associated with oyster mushroom in Korea. Trichoderma green mould has been one of the most serious diseases of oyster mushroom in Korea. Of these the predominant species are two unrecorded species. We designed as Trichoderma sp. Korean type 1 (Th K1) and Trichoderma sp. Korean type 2 (Th K2), respectively. Th K1 and Th K2 can be distinguished from previously reported Trichoderma species as well as each other in morphological characteristics including growth rate at 35$^{\circ}C$, colony morphology, conidia shape and branch pattern of phialides. Sequence of the ITS region of rDNA, the protein coding translation elongation factor gene(EF-1${\alpha}$), and RNA polymeraseII (RPB2) not only clearly separated Trichoderma sp. Korean types from their closely related T. harzianum biotype but also distinguished them from each other. Analyses of the EF-1${\alpha}$ and RPB2 sequences were found to be more useful for establishing systematic relationships among Trichoderma isolates than those of the ITS sequence. Based on the results of morphological and molecular characteristics. We propose the two Trichoderma sp. Korean types as the new species

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Unrecorded Fungal Species Isolated from Medicinal Plant Leaves in Korea (약용식물 잎에서 분리한 국내 미기록 진균)

  • Ahn, Geum Ran;Noh, Hyeungjin;Lee, Dong Hyeung;Kim, Susan;Kim, Jun Young;Kim, Seong Hwan
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.319-328
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    • 2019
  • As an effort to explore fungal diversity, fungal survey was undertaken in 2018 in Jangheung, Korea. For the survey, medicinal plant leaves were sampled from Selaginella tamariscina, Serratula coronata ssp. insularis, Scutellaria baicalensis, Scrophularia buergeriana and subjected to fungal isolation. Four unrecorded fungal species, Paraboeremia selaginellae, Colletotrichum camelliae, Alternaria eichhorniae, and Phomopsis capsici were obtained from the survey. This study described their morphological characteristics including colony features formed on media, light microscopic images and molecular characteristics of nucleotide sequences of the ITS and 28S rDNA regions.