• 제목/요약/키워드: Colony characteristics

검색결과 413건 처리시간 0.025초

자연계로부터 Erythritol 생산 균주의 분리 (Isolation of Erythritol Producing Microorganisms from Nature)

  • 이광준;주영란;이길웅;오경수;이윤진;박상희;임재윤
    • 미생물학회지
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    • 제33권1호
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    • pp.38-42
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    • 1997
  • 자연계로부터 erythritol 생산균주를 선발하기 위하여 옥수수밭이나 사탕수수밭의 토양이나 자판기와 같은 당이 많은 지역으로부터 sampling하여 검색한 결과 erythritol을 생산하는 약 200여 균주를 분리할 수 있었으며 이들을 paper chromatography법으로 1차 선별한 후 HPLC를 이용하여 배양액 중의 erythritol 생산량을 측정함으로서 생산성이 가장 우수하고 부생성물이 가장 적은 KJ81균주를 최종 선발하였다. 선발된 균주의 형태학적 생리학적 특성을 조사하여 penicillium sp. KJ81로 동정하였다. 균주의 콜로니 색은 백색에서 녹색으로 변화하였고 1-3단계 분기의 분생자병과 플라스크형의 경자를 보였다.

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Leaf Spot of Cotton Rose Caused by Corynespora cassiicola in Korea

  • Kwon, Jin-Hyeuk;Park, Chang-Seuk
    • Mycobiology
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    • 제31권1호
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    • pp.57-59
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    • 2003
  • A leaf spot of cotton rose(Hibiscus mutabilis) occurred severely in the flower beds of cotton rose around Uiryeong-gun, Gyeongnam Province in Korea. The causal fungus was identified as Corynespora cassiicola on the basis of cultural and morphological characteristics of the fungus. The fungus grew well on potato dextrose agar and the colony color was gray to brown. Conidia were solitary or catenate, obclavate to cylindrical in shape, and pale olivaceous brown or brown in color. They had 420 pseudosepta, and measured $35.2{\sim}173.6{\times}8.8{\sim}19.9{\mu}m$. Conidia germinated as a bipolar type. Conidiophores were pale to mid brown in color, and measured $74.2{\sim}275.6{\times}3.8{\sim}10.8{\mu}m$. Optimal temperature for mycelial growth was $30^{\circ}C$. The fungal isolate grown on PDA showed strong pathogenicity to cotton rose plant. This is the first report on the corynespora leaf spot of cotton rose(Hibiscus mutabilis) caused by Corynespora cassiicola in Korea.

Penicillifer diparietisporus: a New Record from Field Soil in Korea

  • Das, Kallol;Back, Chang-Gi;Lee, Seung-Yeol;Jung, Hee-Young
    • 한국균학회지
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    • 제46권3호
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    • pp.227-233
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    • 2018
  • A fungus was isolated from field soil collected from Daegu, Korea. The colony of the isolated fungus showed short, branched, and light to dark yellow pigments with hyaline, yellowish red to orange brown aerial mycelia. In addition, the fungus produced solitary to aggregated perithecia, ovoid to pyriform, short neck, and asci as well as biseriately arranged ascospores. Phylogenetic analysis using the internal transcribed spacer region and translation elongation factor $1-{\alpha}$ sequences and morphological characteristics identified the isolated fungus as Penicillifer diparietisporus, which belongs to the family Nectriaceae. To our knowledge, this is the first report of Penicillifer diparietisporus in Korea.

S. peucetius subsp. caesius와 S. platensis의 원형질체 융합 및 융합균주의 분리 (The Interspecific Protoplast Fusion between S. peucetius subsp. caesius and S. platensis)

  • 임미송;이강만
    • 약학회지
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    • 제38권6호
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    • pp.696-702
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    • 1994
  • An interspecific fusant strain, MS1, was obtained by protoplast fusion between S. peucetius subsp. caesius and S. platensis. We studied on the microbiological and cultural characteristics of the fusant MS1. In liquid culture, the viscosity of culture broth of S. peucetius increased during incubation However the fusant MS1 formed pellet like S. platensis without viscosity change. On agar medium the colony morphology of MSI resembled S. platensis but the color was similar to S. peucetius. The fermentation products of the fusant MS1 was identical with S. peucetius.

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섬유소 분해세균의 균학적 성질(제1보) -보존균주에 대한 배양적 성질 및 동정에 대하여- (Characterization of Aerobic Cellulolytic Bacteria Part 1. Cultural Characteristics and Classification of Some Stock Cultures)

  • 최우영
    • 농업과학연구
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    • 제4권2호
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    • pp.167-172
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    • 1977
  • 스코트랜드 National Collection of Industrial Bacteria의 3개 섬유소 분해세균에 대하여 형태적, 생리적, 및 배양적성질을 검토한 결과 NCIB 8077은 Cellulomonas sp.로, NCIB 8633 및 NCIB 8634는 Pseudomonas sp로 각각 분류되었으며 기왕에 보고된 다른 섬유소 분해세균에 대한 분류학상의 문제점을 아울러 고찰하였다.

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Identification, Growth and Pathogenicity of Colletotrichum boninense Causing Leaf Anthracnose on Japanese Spindle Tree

  • Lee, Hyang-Burm;Park, Jae-Young;Jung, Hack-Sung
    • The Plant Pathology Journal
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    • 제21권1호
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    • pp.27-32
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    • 2005
  • Leaf anthracnose was observed on leaves of Japanese spindle tree in Seoul, Korea from autumn 2003 to spring 2004. The causal fungus was purely isolated from he leaf spot lesions and cultured on PDA. The colony on PDA was cream to orange but blackish in the center n old cultures. Conidia were formed in blackish orange asses and were cylindrical in shape, measured 13-17${\times}$5-7 ${\mu}$m in size. Blackish brown setae were often observed on PDA and ranged up to 100 ${\mu}$m in length. Based on morphological and ITS region sequence analyses, the fungal strain was identified as Colletotrichum boninense. Koch’s postulates were fulfilled by inoculating tree leaves with 1 ${\times}$ $106^6$ conidia per ml in a moist chamber. This is the first study on the pathogenicity, growth and phylogenetic characteristics of C. boninense causing leaf anthracnose on Japanese spindle tree in Korea.

Sooty Mold of Persimmon (Diospyros kaki) Caused by Cladosporium cladosporioides

  • Kwon, Jin-Hyeuk;Park, Chang-Seuk
    • The Plant Pathology Journal
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    • 제19권5호
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    • pp.266-268
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    • 2003
  • In 2002, sooty mold was observed on persimmon (Diopyros kald) from the Jinju Agricultural Products Wholesale Market in Gyeongnam, Korea. The lesion of the sooty mold usually appeared as scars or wounds formed on the surface of the fruits. The symptom started with water soaking lesion, then the fruit softened rapidly. Colony of the causal pathogenic fungus was dark green in color on potato dextrose agar. The diameter of growing hyphae was 1-2 $\mu\textrm{m}$. The mostly one-celled conidia were ovoid, lemon or cylinder in shape, blastophores, and sized 46-132 $\mu\textrm{m}$. Erected conidiophores were long-branched chains and their lengths varied from 24 to 346 $\mu\textrm{m}$ with 2-5 $\mu\textrm{m}$ width. The optimum temperature for mycelial growth was about 2$0^{\circ}C$. Based on their mycological characteristics, the fungus was identified as Cladosporium cladosporioides. This is the first report in Korea of the sooty mold of persimmon caused by the said fungus.

A report of unrecorded bacterial species of Korea isolated in 2016, belonging to the family Deinococcaceae and Planctomycetaceae

  • Kim, Dong-Uk;Kim, Ju-Young;Cha, Chang-Jun;Kim, Wonyong;Kim, Myung Kyum
    • Journal of Species Research
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    • 제7권1호
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    • pp.9-12
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    • 2018
  • In 2016, as part of a larger effort to discover indigenous prokaryotic species in Korea, we isolated the family Deinococcaceae and Planctomycetaceae as unrecorded bacterial species. From the high 16S rRNA gene sequence similarity (>98.5%) and formation of a robust phylogenetic clade with known species, it was determined that each strain was a distinct bacterial species. There are no official reports that these two species have been described in Korea; therefore, the bacterial strains of Deinococcus and Blastopirellula are described for the first time in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.

사과에서 분리한 Colletotrichum acutatum, C. gloeosporioides 및 Glomerella cingulata의 병원학적 특징 (Etiological Characteristivcs of Colletotrichum acutatum, C. gloeosporioides and Glomerella cingulata Isolated from Apples)

  • 이두형
    • 한국식물병리학회지
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    • 제10권2호
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    • pp.105-111
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    • 1994
  • Pathogenic fungal species associated with apple anthracnose were studied with diseased fruits collected over two major apple growing areas from 1986 to 1988. Colletotrichum acutatum Simmonds, c. gloeosporioides(Penz.) Sacc. and Glomerella cingulata(Stonem.) Spauld. & Schrenk were identified based on their mycological characteristics. C. acutatum could be easily differentiated from C. gloeosporioides by producing their fusiform conidia and developing apricot∼salmon colony colors, non-forming an ascigerous stages. Growth rate of C. acutatum were slow in plate culture. The three species of anthracnose fungi tested caused apple rot. Symptoms produced by C. gloeosporioides were larger than those of the others. C. gloeosporioides and G. cingulata produced rot lesions on apples that appeared became brown and later black, but C. acutatum produced rot lesions appeared yellowish brown and later reddish brown. G. cingulata has the major species responsible for apple anthracnose, epidemics in Korea.

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Identification of Aspergillus nidulans from cooked eggs produced by permitted factory

  • Park, Hye-Won;Kim, Kook-Joo;Youk, Ji-Hea;Woo, Jong-Tae
    • 한국동물위생학회지
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    • 제30권2호
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    • pp.283-286
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    • 2007
  • Fungus generally doesn't produce toxic or harmful substances so it has low chances to cause food poisoning. However it leads to change appearance, odor and characteristics of the contaminated foods and result in sanitary risk problems. Therefore the contamination of fungi should be prevented since they are not proper for human consumption. Green fungi with white outline raised from the air cell of cooked eggs which were collected by Gyeongi Livestock Veterinary Service in August, 2006. The results came out after the cultivation using Sabouraud's Dextrose Agar(SDA). The conidium appeared white and monospore, the shape of colony was round and oval. Conidiophore was brown and granulated and wrinkles and formed. It was confirmed as Aspergillus nidulans based on the dying using Lactophenol cotton blue, the observation of septum and vesicle from the grown spores, and rDNA sequencing.