• Journal of Microbiology and Biotechnology
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• 제23권4호
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• pp.534-538
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• 2013

The effects of lights with different wavelengths on the growth and the yield of extracellular polysaccharides of Nostoc flagelliforme cells were investigated in a liquid cultivation. N. flagelliforme cells were cultured for 16 days in 500 ml conical flasks containing BG11 culture medium under $27{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ of light intensity and $25^{\circ}C$ on a rotary shaker (140 rpm). The chlorophyll a, phycocyanin, allophycocyanin, and phycoerythrin contents in N. flagelliforme cells under the lights of different wavelengths were also measured. It was found that the cell biomass and the yield of polysaccharide changed with different wavelengths of light. The biomass and the yield of extracellular polysaccharides under the red or violet light were higher than those under other light colors. Chlorophyll a, phycocyanin, and allophycocyanin are the main pigments in N. flagelliforme cells. The results showed that N. flagelliforme, like other cyanobacteria, has the ability of adjusting the contents and relative ratio of its pigments with the light quality. As a conclusion, N. flagelliforme cells favor red and violet lights and perform the complementary chromatic adaptation ability to acclimate to the changes of the light quality in the environment.

• Journal of Microbiology and Biotechnology
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• 제29권1호
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• pp.160-170
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• 2019

The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively for vaccine delivery. Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c). The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs. After incubation with BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more efficient expression of GFP compared with wild-type strains shown by flow cytometry analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum to BMDCs. Similar results were also observed in an in vivo study, which showed that oral administration resulted in efficient expression of GFP in both Peyer's patches (PP) and mesenteric lymph nodes (MLNs) within 7 days after the last administration. In addition, the CD11c-targeting strain significantly promoted the differentiation and maturation of DCs, the differentiation of $IL-4^+$ and $IL-17A^+$ T helper (Th) cells in MLNs, as well as production of $B220^+$ $IgA^+$ B cells in the PP. In conclusion, this study developed a novel DC-targeting L. plantarum strain which could increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a promising approach for vaccine study.

• 대한방사선기술학회지:방사선기술과학
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• 제18권2호
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• pp.87-102
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• 1995

The education of radiologic technology began in the regular institute of higher education in Korea in 1963. Up to now from then, our education to bring up the radiologic technologists has developed greatly in quality and quantity, and now departments of radio-technology are founded in the 16 junior colleges in March, 1995. This study was done to verify the necessity and propriety to reform the education system of radiologic technology which was run as two or three year system of college curriculum for 32 years since 1963, and to search for the method to reform in the future. We got the following results from this research. 1. In the survey, on the desirable education year for radiologic technologists, 63.9 % of professors of department of radio-technology and 63.0 % of radiologic technologists chose the 4 year system, 27.9 % of professors and 34.6 % of radiologic technologists chose the 4 year system added to graduate school. 2. In the survey, on the future development of radiologic equipments and technique, 67.2 % of professors of department of radiologic technology and 86.4 % of radiologic technologists have a view of "revolutional development". Also, on the future tasks or roles of radiologic technologists 95.1% of professors and 94.9% of radiologic technologists have a view that "They will increase". 3. On the necessity of extension of education year to 4 years from 3 years, the factor is that development of medical technique and machinery and tools, advance of qualification of radiologic technologists, enlargement and specialization of the business of the radiologic technologists, ballance of education year with other medical sciences, international competitive power and cooperation of radiologic technology, and education continuation of the graduates of department of radiologic technology. 4. They suggested that in the 4 year curriculum of department of radiologic technology, clinical medicine, quality control of radiation and radiologic equipment, related subjects to the radiologic application and computer application should be enforced and clinical practice should be extended more. 5. On the device to found the 4 year college curriculum of radiologic technology, they suggested that first, 4 year curriculum should be founded through the variety of educational year, secondly, department of radiologic technology should be founded in the 4 year health sciences college, thirdly, educational continuation of the radiologic technologists should be systematized on the basis of life-long education.

• BMB Reports
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• 제43권7호
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• pp.491-498
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• 2010

Chemerin is a novel adipokine which is abundant in adipose tissue to promote adipocyte differentiation and with significant relativity to BMI and insulin sensitivity. We report here the molecular characterization of porcine chemerin and its receptors ChemR23 and GPR1, as well as their transcriptional regulation during lipogenesis. Chemerin was mainly expressed in liver, intestine, kidney and adipose tissue, consistent with the expression pattern of GPR1, but not ChemR23, which was predominantly present in spleen and temperately in adipose tissue. We further investigated the lipogenesis-related transcriptional activation of $PPAR{\gamma}$ and KLF15 on chemerin and its receptors. The data showed that KLF15, but not $PPAR{\gamma}$, can up-regulate the mRNA level of chemerin, ChemR23 and GPR1, which was consistent with the results of luciferase assay that confirmed the effect of KLF15 on ChemR23 promoter. Taken together, our data provide basic molecular information for the further investigation on the function of chemerin in lipogenesis.

• Advances in nano research
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• 제15권3호
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• pp.253-261
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• 2023

This study investigates the application of nano-composite materials in physical education, specifically focusing on improving the performance of sports hall flooring. The research centers on carbon nanotube reinforced polyvinyl chloride (PVC) composites, which offer enhanced mechanical properties and durability. The incorporation of carbon nanotubes as reinforcements in the PVC matrix provides notable benefits, including increased strength, improved thermal stability, electrical conductivity, and resistance to fatigue. The key parameters examined in this study are the weight percentage of carbon nanotubes and the temperature during the fabrication process. Through careful analysis, it is found that higher weight percentages of carbon nanotubes contribute to a more uniform dispersion within the PVC matrix, resulting in improved mechanical properties. Additionally, higher fabrication temperatures aid in repairing macroscopic defects, leading to enhanced overall performance. The findings of this study indicate that the utilization of carbon nanotube reinforced PVC composites can significantly enhance the strength and durability of sports hall flooring. By employing these advanced materials, the safety and suitability of physical education environments can be greatly improved. Furthermore, the insights gained from this research can contribute to the optimization of composite material design and fabrication techniques, not only in the field of physical education but also in various industries where composite materials find applications.

• Journal of Ginseng Research
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• 제45권6호
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• pp.665-675
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• 2021

Background: Ginsenoside Rg1 (Rg1), an active ingredient in ginseng, may be a potential agent for the treatment of Alzheimer's disease (AD). However, the protective effect of Rg1 on neurodegeneration in AD and its mechanism of action are still incompletely understood. Methods: Wild type (WT) and APP/PS1 AD mice, from 6 to 9 months old, were used in the experiment. The open field test (OFT) and Morris water maze (MWM) were used to detect behavioral changes. Neuronal damage was assessed by hematoxylin and eosin (H&E) and Nissl staining. Immunofluorescence, western blotting, and quantitative real-time polymerase chain reaction (q-PCR) were used to examine postsynaptic density 95 (PSD95) expression, amyloid beta (Aβ) deposition, Tau and phosphorylated Tau (p-Tau) expression, reactive oxygen species (ROS) production, and NAPDH oxidase 2 (NOX2) expression. Results: Rg1 treatment for 12 weeks significantly ameliorated cognitive impairments and neuronal damage and decreased the p-Tau level, amyloid precursor protein (APP) expression, and Aβ generation in APP/PS1 mice. Meanwhile, Rg1 treatment significantly decreased the ROS level and NOX2 expression in the hippocampus and cortex of APP/PS1 mice. Conclusions: Rg1 alleviates cognitive impairments, neuronal damage, and reduce Aβ deposition by inhibiting NOX2 activation in APP/PS1 mice.

• 한국치위생학회지
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• 제11권2호
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• pp.223-232
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• 2011

Objectives : To provide basic data for preparing effective CPR guidelines and educational programs for dental hygiene students. Methods : Second-year dental hygiene students in the classes of 5 colleges in Seoul and Gyeonggi-Do from May, 2010 to September, 2010 were enrolled in this study. The subjects comprised of 113 students who had not received CPR education and 134 students who had. Results : 1. The subjective understanding of CPR increased significantly from 52.2% before the education program to 81.4% after the course. 2. The mean level of knowledge of CPR increased significantly from 3.57 before the education program to 6.10 after the course. 3. The attitude to CPR increased significantly from 64.6% before the education program to 86.6% after the program. 4. The appropriate CPR education timing determined by the subjects was high school(36.3%) and college(27.4%) before the education program but elementary school(38.1%) and high school(26.9%) after the course. Conclusions : Continuous CPR education and the development of a CPR educational program in the curriculum of a dental hygiene department are needed. This will help dental hygiene students perform correct CPR when they encounter cardiac arrest patients.

• 한국간호교육학회지
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• 제22권1호
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• pp.51-62
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• 2016

Purpose: This study was to verify the effects of spiritual care module education programs by applying it to nurses. Method: The study employed a non-equivalent control group pretest-posttest design in a quasi-experimental basis. Subjects were 93 nurses (46 in an experimental group and 47 in a control group) with more than two years clinic experience, attending a bachelor program of K University, in I city, Korea. The program consists of courses with 2.5 hours per week for seven weeks. Result: Scores of spiritual needs and spiritual nursing competence increased significantly in the experimental group. The score of spirituality and spiritual well-being also increased in the experimental group, but not significantly. Conclusion: The spiritual care module education program was considered to be an effective nursing intervention education course. Nurses educated with this program seemed to perform better nursing interventions for subjects facing difficulties or confusion by helping them restore and cope with those problems by themselves. Therefore, it is recommended that spiritual care module education should be settled as a regular course of nursing college with consideration to the corrections and supplements mentioned in this study.

• 의학교육논단
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• 제13권2호
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• pp.47-57
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• 2011

Purpose: This study was conducted to explore the current usage of high integration mannequin human patient simulators (hereafter HPS) in nursing education. Methods: Thirty-two faculty members in 11 nursing schools participated in this survey. the questionnaire consisted of some multiple-choice questions and open questions to collect data on the experience of faculty members with using HPS as well as quantitative information. Results: The faculty members who used HPS in their classes recognized the need to use simulation in nursing education. They identified the advantages of simulation education as promoting adaptation to the clinical environment(46.9%), promoting self-confidence(28.1%), promoting critical thinking(28.15), and making direct nursing skill practice available(28.1%). However, they considered the obstacles to the use of simulation in nursing education to be insufficient manpower(62.5%), insufficient time(40.6%), and difficulty in preparing a teaching strategy(34.4%). Conclusion: HPS is useful in nursing education, but systematic plans and policies regarding on its operation are needed to confirm its effectiveness. The results of this study generally provide a basic information for use of HPS in nursing education.

• 대한지역사회영양학회지
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• 제12권6호
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• pp.742-751
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• 2007

The purpose of study was to investigate the utilization of environment-friendly agricultural products of the college students attending nutrition education. The subjects were 387 college students (male 53.2%, female 46.8%) at universities in the Seoul and Incheon areas. A cross-sectional study was conducted by a self-administered questionnaire. The percentage of subjects who purchased environment-friendly agricultural products was 37.2%. The most purchased environment-friendly agricultural products were vegetables. The main reason for preferring the environment-friendly agricultural products was good for health (53.7%). Also, the main motive of purchasing the environment-friendly agricultural products was for health (87.5%). For the quality of the environment-friendly agricultural products, they answered 'safety' (4.19), 'freshness' (4.03) and 'nutrition' (3.90), respectively. For college students' right choices of environment-friendly agricultural products, information and consumer education on these products are necessary.