• Journal of Dairy Science and Biotechnology
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• v.34 no.4
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• pp.239-244
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• 2016

The roots of Cichorium intybus L. (chicory) have many nutritional qualities and also have been used for health promotion as traditional medicine. The antimicrobial activity of Cichorium intybus L.'s (chicory) ethanol extract on Bacillus cereus ATCC 10876 were investigated using spot on lawn method in this study. Ethanol extract exhibited antimicrobial activities against Bacillus cereus ATCC 10876 with significance. Also, the results produced the larger zones of inhibition against Bacillus cereus ATCC 10876 tested, with increasing concentration of Cichorium intybus L.'s (chicory) ethanol extracts,. Hence, Cichorium intybus L. (chicory) could be served for keeping the safety of various dairy products as natural antimicrobial agents.

• Biomolecules & Therapeutics
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• v.27 no.2
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• pp.193-200
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• 2019

Ceramide metabolism is known to be an essential etiology for various diseases, such as atopic dermatitis and Gaucher disease. Glucosylceramide synthase (GCS) is a key enzyme for the synthesis of glucosylceramide (GlcCer), which is a main ceramide metabolism pathway in mammalian cells. In this article, we developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine GCS activity using synthetic non-natural sphingolipid C8-ceramide as a substrate. The reaction products, C8-GlcCer for GCS, could be separated on a C18 column by reverse-phase high-performance liquid chromatography (HPLC). Quantification was conducted using the multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z $588.6{\rightarrow}264.4$ for C8-GlcCer at positive ionization mode. The calibration curve was established over the range of 0.625-160 ng/mL, and the correlation coefficient was larger than 0.999. This method was successfully applied to detect GCS in the human hepatocellular carcinoma cell line (HepG2 cells) and mouse peripheral blood mononuclear cells. We also evaluated the inhibition degree of a known GCS inhibitor 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP) on GCS enzymatic activity and proved that this method could be successfully applied to GCS inhibitor screening of preventive and therapeutic drugs for ceramide metabolism diseases, such as atopic dermatitis and Gaucher disease.

• Journal of the Korean neurological association
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• v.36 no.4
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• pp.337-340
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• 2018

Human immunodeficiency virus (HIV) infection can result in ischemic stroke via several mechanisms, including opportunistic infection, vasculopathy, cardioembolism, and coagulopathy. HIV-vasculopathy is related to endothelial dysfunction, stenosis and aneurysm formation, infectious vasculitis, dissection and accelerated atherosclerosis during highly active antiretroviral therapy (HAART). We represent a case of HIV infection manifested as an acute ischemic stroke attack. After 4 months during HAART, our patient experienced a recurrent ischemic stroke with progression of middle cerebral artery stenosis.

• Biomedical Science Letters
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• v.28 no.4
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• pp.317-321
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• 2022

Due to COVID-19 pandemic, wearing face masks is obligatory to prevent respiratory virus transmissions in the community. However, there are few studies of the desirable number of wearing a face mask, and how to store them for reuse. Therefore, in this study, a survey was conducted among 208 healthy adults, and 27 kf-94 masks worn for 1, 2, and 3 days were collected. To estimate the risk of bacterial contamination, we analyzed the extent of bacterial contamination of the BHI medium and 16S rRNA gene sequencing. With an increase in the number of days of using the mask, the degree of bacterial contamination of the used mask gradually increased. As a result of 16S rRNA PCR performed for strain identification, Staphylococcus, known as a pathogenic bacterium, was identified the most. In conclusion, we found that wearing a cotton KF mask provides an optimal environment for microbes, which are related to the skin and respiratory system, to thrive. Therefore, it is also important to reduce the risk of bacterial infection of the face mask with appropriate sterilization methods.

• Korean Journal of Health Education and Promotion
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• v.28 no.5
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• pp.1-16
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• 2011

Objectives: A healthy campus enables students to pursue their academic goals. This study examines the health in a broad spectrum of university students and suggests a systematic approach to building a healthy university campus. Methods: The Korea National Health and Nutrition Examination Survey (1998-2009) results and the American College Health Association-National College Health Assessment (2000-2011) report were used as bases for this study. Results: The most common health problems were allergies, sinusitis, ear infections, anemia, depression, and stress among both American students and Korean young people in the 19-29 age group. American students were more likely to be overweight, while Korean young people were to become underweight. College students were also less likely to practice healthy behavior(i.e. vaccinations, physical activity, and not smoking). To build a healthy university campus, six areas of health services are suggested: medical care, health education, counseling, immunization, heath promotion, and employee assistance programs (EAP). In addition, universities should develop effective strategies to improve health on campus, such as community partnerships. Conclusions: To make the most of the limited resources requires a systematic approach that focuses on continuous monitoring of health on campus, health surveys, and collaboration between universities and their communities.

• 대한예방의학회:학술대회논문집
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• 2005.10a
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• pp.328-328
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• 2005

• 대한예방의학회:학술대회논문집
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• 1999.10a
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• pp.273-274
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• 1999

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.131-132
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• 2008

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.129-130
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• 2008

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.417-418
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• 2008