• Journal of Ginseng Research
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• 제47권1호
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• pp.44-53
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• 2023

Background: The genus Panax in the Araliaceae family has been used as traditional medicinal plants worldwide and is known to biosynthesize ginsenosides and phytosterols. However, genetic variation between Panax species has influenced their biosynthetic pathways is not fully understood. Methods: Simultaneous analysis of transcriptomes and metabolomes obtained from adventitious roots of two tetraploid species (Panax ginseng and P. quinquefolius) and two diploid species (P. notoginseng and P. vietnamensis) revealed the diversity of their metabolites and related gene expression profiles. Results: The transcriptome analysis showed that 2,3-OXIDOSQUALENE CYCLASEs (OSCs) involved in phytosterol biosynthesis are upregulated in the diploid species, while the expression of OSCs contributing to ginsenoside biosynthesis is higher in the tetraploid species. In agreement with these results, the contents of dammarenediol-type ginsenosides were higher in the tetraploid species relative to the diploid species. Conclusion: These results suggest that a whole-genome duplication event has influenced the triterpene biosynthesis pathway in tetraploid Panax species during their evolution or ecological adaptation. This study provides a basis for further efforts to explore the genetic variation of the Panax genus.

• Journal of Microbiology and Biotechnology
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• 제22권4호
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• pp.501-509
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• 2012

A 990 bp full-length gene (xynAHJ2) encoding a 329-residue polypeptide (XynAHJ2) with a calculated mass of 38.4 kDa was cloned from Bacillus sp. HJ2 harbored in a saline soil. XynAHJ2 showed no signal peptide, distinct amino acid stretches of glycoside hydrolase (GH) family 10 intracellular endoxylanases, and the highest amino acid sequence identity of 65.3% with the identified GH 10 intracellular mesophilic endoxylanase iM-KRICT PX1-Ps from Paenibacillus sp. HPL-001 (ACJ06666). The recombinant enzyme (rXynAHJ2) was expressed in Escherichia coli and displayed the typical characteristics of low-temperature-active enzyme (exhibiting optimum activity at $35^{\circ}C$, 62% at $20^{\circ}C$, and 38% at $10^{\circ}C$; thermolability at ${\geq}45^{\circ}C$). Compared with the reported GH 10 low-temperature-active endoxylanases, which are all extracellular, rXynAHJ2 showed low amino acid sequence identities (<45%), low homology (different phylogenetic cluster), and difference of structure (decreased amount of total accessible surface area and exposed nonpolar accessible surface area). Compared with the reported GH 10 intracellular endoxylanases, which are all mesophilic and thermophilic, rXynAHJ2 has decreased numbers of arginine residues and salt bridges, and showed resistance to $Ni^{2+}$, $Ca^{2+}$, or EDTA at 10 mM final concentration. The above mechanism of structural adaptation for low-temperature activity of intracellular endoxylanase rXynAHJ2 is different from that of GH 10 extracellular low-temperature-active endoxylanases. This is the first report of the molecular and biochemical characterizations of a novel intracellular low-temperature-active xylanase.

• Journal of Ecology and Environment
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• 제30권3호
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• pp.271-276
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• 2007

A simple and efficient protocol was developed for culturing Cu-tolerant and Cu-accumulating plants via pre-adaptation to Cu during plant tissue culture. We induced multiple shoots from begonia (Begonia evansiana Andr.) leaf explants on MS medium supplemented with naphtaieneacetic acid and benzyladenine. After 3 months, small plantlets were transferred to MS medium supplemented with $100{\mu}M\;CuCl_2$ for pre-adaptation to Cu and cultured for 5 months. Then, these plantlets were individually planted in pots containing artificial soil. An additional 500 mg of Cu dissolved in 1/4 strength MS solution was applied to each pot during irrigation over the course of 2 months. We planted pre-adapted and control begonias in soil from the II-Kwang Mine, an abandoned Cu mine in Pusan, Korea, to examine their ability to tolerate and accumulate Cu for phytore-mediation. Pre-adapted begonias accumulated $1,200{\mu}g$ Cu/g dry root tissue over the course of 45 days. On the other hand, non-Cu-adapted controls accumulated only $85{\mu}g$ Cu/g dry root tissue. To enhance Cu extraction, chelating agents, ethylenediamine tetraacetic acid (EDTA)-dipotassiun and pyridine-2,6-dicarboxylic acid (PDA), were applied. While the chelating agents did not enhance accumulation of Cu in the roots of control begonias, EDTA application increased the level of Cu in the roots of pre-adapted begonias twofold (to $2,500{\mu}g$ Cu/g dry root tissue). Because pre-adapted begonias accumulated a large amount of Cu, mainly in their roots, they could be used for phytostabilization of Cu-contaminated soils. In addition, as a flowering plant, begonias can be used to create aesthetically pleasing remediation sites.

• Journal of Animal Science and Technology
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• 제61권4호
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• pp.239-244
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• 2019

This study aimed to determine the effective dose of intravenous administration of L-tryptophan (L-T) on gastrointestinal hormones (GIH) secretions and melatonin using Hanwoo cattle. Three steers ($362{\pm}23kg$) fitted with indwelling jugular vein catheters were assigned in a $3{\times}3$ Latin square design. Treatments were intravenous administration of saline (control), 28.9 mg L-T/kg body weight (BW; low) and 57.8 mg L-T/kg BW (high) L-T for 1 day with 7 days of adaptation. Samples were collected after adaptation period at -60, 0, 30, 60, 90, 120, 150, 180, 240, and 300 min of sampling day. The levels of serum cholecystokinin (CCK) and secretin were higher (p < 0.05) in the high L-T group than those in the other groups. Serum Melatonin (MEL) levels were increased upon L-T administration (p < 0.05) in the high L-T group. Taken together, the effective dose of L-T administration was defined at 57.8 mg L-T/kg BW in order to stimulate increase of GIH and MEL.

• 대한한의학원전학회지
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• 제24권4호
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• pp.175-193
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• 2011

In present-day Korea, a climatic change is causing various changes to the life of people and their body. The climate is more and more warm, so the internal environment of people's body is exchanging to warm. The climatic change's range is big and the speed is fast. So the harmony of human body is being destroyed and the human body's adaptation ability is becoming low. At this condition, diseases more frequently occur. So doctor must expect the occur of diseases and practice early treatment. And in present-day Korea, a life environmental change is being advanced. The life environmental change is causing big changes to the people's body and having influences to the occur of diseases. To this problem, doctor must expect and practice early treatment. This thesis would like to interpret about present-day Korea's climatic changes and life environmental changes in Korean Medicine point of view, and try to present the method of Korean Medicine early treatment. And comment on present-day Korea's early treatment method especially on health supplement or functional food and vaccination.

• 대한치위생과학회지
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• 제6권2호
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• pp.161-172
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• 2023

연구배경: 치위생과 학생들의 자아인식과 타인인식, 대학생활 적응 정도를 알아보고 대학생활 적응에 미치는 요인을 파악하고자 한다. 연구방법: 치위생과 재학생 191명을 대상으로 설문조사에 동의를 구하고 G*power 3.1.9.4 program으로 효과 크기를 산출하였다. 일반적 특성에 따른 자기인식, 타인인식, 대학생활적응의 차이와 상관관계 및 대학생활 적응에 영향요인을 알아보기 위해 독립 표본 t-test 및 일원 배치 분산분석, 사후분석 scheffe, 상관분석과 다중회귀분석을 실시하였다. 연구결과: 대학생활 적응은 자기인식(r=.301)에서 유의한 정(+)의 상관관계가 나타났고, 하위 요인과의 관련성은 사회적 적응과 사적 자기인식(r=.340), 공적 자기인식(r=.331)에서 가장 높은 정(+)의 상관관계가 나타났다(p<0.01). 타인인식(r=-.051)은 대학생활 적응과 부(-)의 관련성을 보였으나 통계적으로 유의한 차이는 나타나지 않았다. 대학생활 적응 요인으로는 사적 자기인식(β=.232)과 공적 자기인식(β=.254)이 정(+)의 영향을 주었고, 내적 타인인식(β=-.205)이 부(-)의 영향을 주는 것으로 나타났다(p<0.01) 결론: 치위생과 학생들의 자기인식과 타인인식은 원만한 대학생활 적응에 영향요인으로 대학 생활의 적응력과 전공 직무와 연계한 핵심 역량 배양을 위해 향상 방안이 고려되어야 할 것이다.

• Journal of Animal Science and Technology
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• 제63권6호
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• pp.1411-1422
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• 2021

Lactobacillus acidophilus is a gram-positive, microaerophilic, and acidophilic bacterial species. L. acidophilus strains in the gastrointestinal tracts of humans and other animals have been profiled, but strains found in the canine gut have not been studied yet. Our study helps in understanding the genetic features of the L. acidophilus C5 strain found in the canine gut, determining its adaptive features evolved to survive in the canine gut environment, and in elucidating its probiotic functions. To examine the canine L. acidophilus C5 genome, we isolated the C5 strain from a Korean dog and sequenced it using PacBio SMRT sequencing technology. A comparative genomic approach was used to assess genetic relationships between C5 and six other strains and study the distinguishing features related to different hosts. We found that most genes in the C5 strain were related to carbohydrate transport and metabolism. The pan-genome of seven L. acidophilus strains contained 2,254 gene families, and the core genome contained 1,726 gene families. The phylogenetic tree of the core genes in the canine L. acidophilus C5 strain was very close to that of two strains (DSM20079 and NCFM) from humans. We identified 30 evolutionarily accelerated genes in the L. acidophilus C5 strain in the ratio of non-synonymous to synonymous substitutions (dN/dS) analysis. Five of these thirty genes were associated with carbohydrate transport and metabolism. This study provides insights into genetic features and adaptations of the L. acidophilus C5 strain to survive the canine intestinal environment. It also suggests that the evolution of the L. acidophilus genome is closely related to the host's evolutionary adaptation process.

• 대한간호학회지
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• 제52권3호
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• pp.324-340
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• 2022

Purpose: This study determined acculturative stress' effect on the life satisfaction of multicultural adolescents based on Roy's Adaptation Model and some earlier studies. Further, it examined the sequential multiple mediating effects of bicultural acceptance attitude, self-esteem, and social withdrawal on life satisfaction. Methods: Participants included 1,163 multicultural adolescents who participated in the sixth Multicultural Adolescents Panel Study. A hypothesis test was conducted using Hayes' Process Macro Model 81. Results: Life satisfaction increased with a decline in acculturative stress. Each of bicultural acceptance attitude, self-esteem, and social withdrawal had a single mediating effect on the relationship between acculturative stress and life satisfaction in multicultural adolescents. The sequential multiple mediating effects of bicultural acceptance attitude and self-esteem were confirmed significant after their impact on the relationship between acculturative stress and life satisfaction was analyzed. Bicultural acceptance attitude and social withdrawal were found to have a significant sequential multiple mediating effect on the relationship, as well. Conclusion: This study's results demonstrate that acculturative stress reduction is critical to improving multicultural adolescents' life satisfaction. Bicultural acceptance attitude, self-esteem, and social withdrawal have a single mediating or sequential multiple mediating effect on the relationship between multicultural adolescents' acculturative stress and life satisfaction. The findings, which highlight mediating effects, indicate that by increasing bicultural acceptance attitude and self-esteem, and reducing social withdrawal, multicultural adolescents' life satisfaction can be improved.

• 한국콘텐츠학회논문지
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• 제20권4호
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• pp.639-647
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• 2020

본 연구의 목적은 의과대학 의예과 신입생들의 셀프리더십, 자아존중감, 대인관계유능감, 대학생활적응의 관계를 분석하여 대학생활적응을 보다 잘 할 수 있도록 돕기 위한 기초자료를 제공하는 것이다. 본 연구의 대상은 C지역 의과대학 의예과 신입생 131명이었다. 연구결과, 셀프리더십은 평균 3.86(±0.61), 자아존중감과 대인관계유능감, 대학생활적응은 평균 3.86(±.61), 3.44(±.48), 3.78(±.52)이였고, 성별은 차이가 없었다(p>0.05). 셀프리더십, 자아존중감, 대인관계유능감, 대학생활적응간에는 정적 상관관계가 나타났다(p<0.05). 셀프리더십은 자기존중감과 대인관계유능감을 매개로 하여 대학생활적응에 간접적으로 영향을 미치고, 자기존중감과 대인관계유능감은 대학생활적응에 직접적으로 영향을 미치며, 자기존중감은 대인관계유능감을 통해 대학생활적응에 간접적으로 영향을 미치는 것으로 나타났다. 본 연구는 의예과 신입생들의 대학생활적응에 영향을 미치는 요인에 대한 정보를 제공하고, 대학생활적응을 위하여 자아존중감은 필수적으로 교육해야 하는 내용임을 확인하였다. 본 연구가 대학생활적응을 돕기 위한 관련 교육프로그램을 개발하고 활성화하는데 활용될 수 있기는 기대한다.

• 기본간호학회지
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• 제25권3호
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• pp.185-196
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• 2018

Purpose: The purpose of this study was to investigate the alopecia experience in adults and to explain the process of their experiences. Methods: Using a grounded theory methodology, 18 interviews were performed with fourteen men and four women, 34~57 years of age, suffering from alopecia. Data were analyzed using the constant comparative analysis method. Results: The core category emerged as "inescapable fetters". adults with alopecia engaged in three stages: embarrassment, seeking solution, and acceptance phase. Causal conditions were a vicious cycle of stress, biological factors and poor life style. Contextual conditions were recognition of irreversibleness, negative social awareness, and marriage. The central phenomenon of the adaptation process among the adults with alopecia was withdrawn life due to negative body image. Action/Interaction strategies included rely on medical treatment, efforts to take good care of hair, research for information treatment, efforts to cover up hair loss, and mind control. Intervening conditions were time cost, economic cost, support of surrounding people. Consequences was burden of unfinished lifetime homework. Conclusion: When caring for these adults, it is important to identify needs, allow patients to express what they want at that moment and support them in maintaining a daily life.