• 제목/요약/키워드: Collapse spectrum

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Evaluation and Genome Mining of Bacillus stercoris Isolate B.PNR1 as Potential Agent for Fusarium Wilt Control and Growth Promotion of Tomato

  • Rattana Pengproh;Thanwanit Thanyasiriwat;Kusavadee Sangdee;Juthaporn Saengprajak;Praphat Kawicha;Aphidech Sangdee
    • The Plant Pathology Journal
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    • 제39권5호
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    • pp.430-448
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    • 2023
  • Recently, strategies for controlling Fusarium oxysporum f. sp. lycopersici (Fol), the causal agent of Fusarium wilt of tomato, focus on using effective biocontrol agents. In this study, an analysis of the biocontrol and plant growth promoting (PGP) attributes of 11 isolates of loamy soil Bacillus spp. has been conducted. Among them, the isolates B.PNR1 and B.PNR2 inhibited the mycelial growth of Fol by inducing abnormal fungal cell wall structures and cell wall collapse. Moreover, broad-spectrum activity against four other plant pathogenic fungi, F. oxysporum f. sp. cubense race 1 (Foc), Sclerotium rolfsii, Colletotrichum musae, and C. gloeosporioides were noted for these isolates. These two Bacillus isolates produced indole acetic acid, phosphate solubilization enzymes, and amylolytic and cellulolytic enzymes. In the pot experiment, the culture filtrate from B.PNR1 showed greater inhibition of the fungal pathogens and significantly promoted the growth of tomato plants more than those of the other treatments. Isolate B.PNR1, the best biocontrol and PGP, was identified as Bacillus stercoris by its 16S rRNA gene sequence and whole genome sequencing analysis (WGS). The WGS, through genome mining, confirmed that the B.PNR1 genome contained genes/gene cluster of a nonribosomal peptide synthetase/polyketide synthase, such as fengycin, surfactin, bacillaene, subtilosin A, bacilysin, and bacillibactin, which are involved in antagonistic and PGP activities. Therefore, our finding demonstrates the effectiveness of B. stercoris strain B.PNR1 as an antagonist and for plant growth promotion, highlighting the use of this microorganism as a biocontrol agent against the Fusarium wilt pathogen and PGP abilities in tomatoes.

Assessment of seismic parameters for 6 February 2023 Kahramanmaraş earthquakes

  • Bilal Balun
    • Structural Engineering and Mechanics
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    • 제88권2호
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    • pp.117-128
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    • 2023
  • On February 6, 2023, Türkiye woke up with a strong ground motion felt in a wide geography. As a result of the Kahramanmaraş, Pazarcık and Elbistan earthquakes, which took place 9 hours apart, there was great destruction and loss of life. The 2023 Kahramanmaraş earthquakes occurred on active faults known to pose a high seismic hazard, but their effects were devastating. Seismic code spectra were investigated in Hatay, Adıyaman and Kahramanmaraş where destruction is high. The study mainly focuses on the investigation of ground motion parameters of 6 February Kahramanmaraş earthquakes and the correlation between ground motion parameters. In addition, earthquakes greater than Mw 5.0 that occurred in Türkiye were compared with certain seismic parameters. As in the strong ground motion studies, seismic energy parameters such as Arias intensity, characteristic intensity, cumulative absolute velocity and specific energy density were determined, especially considering the duration content of the earthquake. Based on the study, it was concluded that the structures were overloaded far beyond their normal design levels. This, coupled with significant vertical seismic components, is a contributing factor to the collapse of many buildings in the area. In the evaluation made on Arias intensity, much more energy (approximately ten times) emerged in Kahramanmaraş earthquakes compared to other Türkiye earthquakes. No good correlation was found between moment magnitude and peak ground accelerations, peak ground velocities, Arias intensities and ground motion durations in Türkiye earthquakes. Both high seismic components and long ground motion durations caused intense energy to be transferred to the structures. No strong correlation was found between ground motion durations and other seismic parameters. There is a strong positive correlation between PGA and seismic energy parameter AI. Kahramanmaraş earthquakes revealed that changes should be made in the Turkish seismic code to predict higher spectral acceleration values, especially in earthquake-prone regions in Türkiye.

119구급대원의 심폐소생술 성적 분석 - 병원전 심정지를 중심으로 - (Factors Affecting the Survivals of Out-of-hospital Cardiac Arrests by 119 Fire Service)

  • 강병우
    • 한국응급구조학회지
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    • 제9권2호
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    • pp.111-128
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    • 2005
  • Background: Cardiac arrest is one of the most critical diseases which can likely lead to severe cerebral disability or brain death when the cases can not recover their circulation within 10 minutes. Saving out-of-hospital cardiac arrest cases is a recent concern in Korea. Resuscitation has become an important multidisciplinary branch of medicine, demanding a spectrum of skills and attracting a plethora of specialities and organizations. The best survival can be achieved if all the following links have been optimized : rapid access, and early CPR, defibrillation and ACLS, Since the "Utstein Style" was advocated in 1991, many reports about out-of-hospital cardiac arrest have been published based on this guideline. These differences prevent valid inter-hospital and international comparisons. However, it is not known how effective resuscitation has become to the patients. In other words, there are no guidelines for reviewing, reporting, and conducting research on resuscitation in Korea. This dissertation aims to provide the basic data for a unified reporting guideline of resuscitation in Korea and evaluating the out-of-hospital factors associated with survival discharge of out-of-hospital cardiac arrest. Methods: As for this study, uses the collected data about Out-of-hospital cardiac arrests at 4 area, from January, 2005 to April. 2005. With a retrospective study, 174 cases were analyzed. The data was recorded based on the Out-of-Hospital Utstein Style. Results: Resuscitation was performed on 174 out-of-hospital cardiac arrest cases at the 4 area 14 patients(8.1%) recovered their spontaneous circulation. Overall, the ROSC of the out-of-hospital cardiac arrest patients was 8.1%, which was poorer than that of western countries. Gender distribution was 50 females(28.7%) and 124 males(71.3%), approximately twice as many males as females. ROSC of witnessed arrests was found out to be 97.7%. The ratio of the witnessed arrest groups showed higher results than that of unwitnessed arrest groups in the above-examined cases. Cardiac etiology consisted of cardiac(33.5%), non-cardiac(45.7%), trauma(20.1%), and unknown(6.0%). Cardiac was the best performance. Initial rhythm showed Ventricular Tachycardia/pulseless Ventricular Fibrillation in 8 patients(6.0%), asystole in 100(75.2%) and unknown in 25(18.8%). The results of the Ventricular Tachycardia/pulseless Ventricular Fibrillation showed higher results than the others cases, The proportion of the cardiogenic cause was 33.5%, which was only half of western countries. Ventricular Tachycardia/pulseless Ventricular Fibrillation is relatively rare. These differences were due to the prevalent pattern of Out-of-hospital cardiac arrest as well as prematurity of the EMSS. Bystander CPR was practiced on 13 patients(7.52%). ROSC was shown in 46.2% cases. CPR by EMT was carried out on 167 cases(96.5%). ACLS by EMf was rare. From collapse, 4 cases(2.6%) arrived to ED within 6 minutes. 13 (8.6%) within 10 minutes, and 49(32.5%) over 31 minutes. The sooner the patients arrived, the greater the ratio of ROSC and discharged alive became, and the same with collapse time to ROSC. As the results of the logistic regression analysis, ROSC was found out to be highly influenced by the time of ED arrival from collapse and Ventricular Tachycardia/pulseless Ventricular Fibrillation. Therefore, the ratio of ROSC depends on not any single factor but various intervention factors. Conclusion: This dissertation presents the following suggestions and directions of the study hereafter. First, the first step for a chain of survival should be taken to activate EMSS early with a phone as soon as cardiac arrests are witnessed. Second, it is keenly needed that emergency medical technicians should be increased through emergency education for living. Third, it is necessary to establish the emergency transportation system. Fourth, most of the Koreans have little understanding of EMT and the present operation systems have many problems, which should be fundamentally changed. Fifth, it is required to have an active medical control over Out-of-hospital CPR, And proper psychological supports should be given not only to patients themselves and their family but also individuals who are engaged in emergency situation. Finally, through studies hereafter on nationwide, comprehensive, and standard forms, it is needed to examine into the biological figures of human body, causes and trends of cardiac arrests, and then, to enhance the survival rate of Out-of-hospital cardiac arrests. Korean guidelines for Cardiopulmonary resuscitation need to be made.

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응답변위법과 응답이력해석법을 이용한 지중 공동구의 내진성능 평가 (Seismic Performance Evaluation of the Underground Utility Tunnel by Response Displacement Method and Response History Analysis)

  • 권기용;이진선;김용규;윤준웅;정순용
    • 한국지반공학회논문집
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    • 제36권11호
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    • pp.119-133
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    • 2020
  • 지중공동구는 대표적인 개착식 지중구조물로, 지진시 지반의 변위로 인한 지진력이 작용하는 구조물이다. 시설안전기술공단에서는 2020년에 기존 시설물(공동구) 내진성능 평가요령을 제정하여 배포하였다. 본 논문에서는 내진성능평가요령에서 제시하고 있는 두 가지 성능평가방법인 응답변위법과 응답이력해석기법을 소개하고, 대표 공동구에 대해서 두 가지 내진성능평가방법을 적용하여 그 결과를 비교·검토 하였다. 평가에 사용된 공동구는 1988년 건설되어 내진설계가 적용되지 않은 공동구 이다. 응답변위법은 기반면 속도응답스펙트럼으로부터 단일코사인과 이중코사인 방법을 적용하여 내진성능평가를 실시하였다. 응답이력해석은 지반과 구조재료 모두 비선형 거동을 나타낼 수 있는 유한차분해석프로그램을 사용하여 2차원 평면변형율 해석을 시행하였다. 붕괴방지 수준의 지진에 대한 성능평가 결과 응답변위법은 구조물이 탄성범위내 거동하는 것으로 평가되는 반면, 응답이력해석은 구조물 일부에서 소성힌지가 발생하는 결과를 나타내었다.

Production of a New Biosurfactant by a New Yeast Species Isolated from Prunus mume Sieb. et Zucc.

  • Jeong-Seon Kim;Miran Lee;Dae-Won Ki;Soon-Wo Kwon;Young-Joon Ko;Jong-Shik Kim;Bong-Sik Yun;Soo-Jin Kim
    • Journal of Microbiology and Biotechnology
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    • 제33권8호
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    • pp.1023-1029
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    • 2023
  • Biosurfactants reduce surface and interfacial tension due to their amphiphilic properties and are an eco-friendly alternative for chemical surfactants. In this study, a new yeast strain JAF-11 that produces a biosurfactant was selected using drop collapse method, and the properties of the extracts were investigated. The nucleotide sequences of the strain were compared with closely related strains and identified based on the D1/D2 domain of the large subunit ribosomal DNA (LSU) and internal transcribed spacer (ITS) regions. Neodothiora populina CPC 39399T, the closest species with strain JAF-11, showed a sequence similarity of 97.75% for LSU and 94.27% for ITS, respectively. The result suggests that the strain JAF-11 represents a distinct species that cannot be assigned to any existing genus or species in the family Dothideaceae. Strain JAF-11 produced a biosurfactant reducing the surface tension of water from 72 mN/m to 34.5 mN/m on the sixth day of culture and the result of measuring the critical micelle concentration (CMC) by extracting the crude biosurfactant was found to be 24 mg/l. The molecular weight 502 of the purified biosurfactant was confirmed by measuring the fast atom bombardment mass spectrum. The chemical structure was analyzed by measuring 1H nuclear magnetic resonance (NMR), 13C NMR, and two-dimensional NMRs of the compound. The molecular formula was C26H46O9, and it was composed of one octanoyl group and two hexanoyl groups to myo-inositol moiety. The new biosurfactant is the first report of a compound produced by a new yeast strain, JAF-11.

대순진리회의 효 윤리에 나타난 종교성 연구 (A Study on the Religiosity of Filial Piety Ethics in Daesoonjinrihoe)

  • 차선근
    • 대순사상논총
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    • 제27집
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    • pp.171-200
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    • 2016
  • 이 글은 전통 효(Filial Piety) 윤리와 대순진리회(Daesoonjinrihoe)의 효 윤리를 비교의 지평에서 분석한 것이다. 그동안 한국의 전통 효 윤리는 현대와 맞지 않는다는 이유로 변화를 요구받아 왔고, 그 핵심은 부모와 자식 간의 관계가 수직적·일방적인 게 아닌 수평적·상호 호혜적이어야 한다는 것이었다. 하지만 이에 대한 구체적인 이념과 대안에 대한 깊이 있는 연구는 거의 없었다. 이런 측면에서 한국의 대표적인 민족종교인 대순진리회가 근대화 이후에 전통과 현대의 충돌을 경험하면서 성장해 왔다는 점을 감안하면, 그 교리체계에서 효 윤리가 어떻게 구축되어 있는지를 관찰하는 작업은 전통 효가 현대적 감각에 맞게 어떻게 윤색될 수 있는지를 살피게 하는 하나의 좋은 사례를 제공해줄 수 있으리라 본다. 이것을 요약하면, 첫째, 유교와 대순진리회는 봉친(奉親)을 효 윤리로 삼지만, 유교는 가부장적인 봉건성에 입각하여 아랫사람의 일방적·맹목적 희생을 강조하고, 대순진리회는 어느 한쪽의 일방적인 희생을 지양하며 부모와 자식 사이의 상생(mutual beneficence)을 도모하고 있다. 이러한 차이는 유교적 효가 봉건질서를 추구하는 이념 속에서, 대순진리회 효가 새로운 종교적 세계인 후천 신세계의 원리인 보은상생과 인존(Respect For Man)의 이념 속에서 구축된 결과로 나타난 것이다. 둘째, 불교와 도교의 효는 부모 생전에 수복(壽福)을 누릴 것을 기원하고 사후에는 천도를 위해 발원하는 소극적·수동적인 것이다. 대순진리회의 효 역시 그러한 관념을 일부 포함하지만, 거기에 그치지 않고 부모 스스로가 죄를 벗고 앞길을 닦아나가도록 유도하는 것까지 효의 범주를 더 넓게 잡는다. 부모와 자식의 수행을 동시에 요구하는 이런 효 윤리는, 자식이 수행 끝에 종교적 목표를 이룬 연후 받게 되는 복록을 부모도 동시에 누릴 수 있게 하고자 하는 대순진리회 세계관 때문에 성립된다. 셋째, 유교와 대순진리회는 선령향화를 효 윤리로 삼지만, 무속적 사고를 배제한 본래 유교(성리학)적 세계관 속의 효는 향화의 대상을 비인격적 존재로, 대순진리회는 인격적 존재로 상정한다. 따라서 유교에 비해 대순진리회의 선령향화는 관념에 치우치지 않는다는 점에서 보다 현실적이다. 넷째, 유교와 대순진리회는 모두 조상들의 은혜를 갚고자 하나 그 은혜의 내용과 보은에 차이가 있다. 유교에서는 조상들이 생명을 준 존재이기에 그에 대한 감사로써 향화를 올리는 것만으로 효가 성립되지만, 대순진리회에서는 조상신들이 자손의 도성덕립을 목적으로 60년 적공(積功)으로써 생명을 주면서 동시에 수도를 하도록 독려하는 존재로 설정되어 있기 때문에 향화를 올리는 것과 더불어 수도까지 해야 효가 성립된다. 다섯째, 유교에서는 세속에서의 출세를 의미하는 입신양명이 효이지만, 대순진리회에서는 그 보다는 수도를 성공시켜 종교적 목표를 달성시키는 것이 더 큰 효로 규정된다. 여섯째, 유불도는 모두 도덕에 기반한 가족윤리로 효를 규정한다. 대순진리회 역시 그러하지만, 그 외에도 효에 종교적 구원을 위한 필수 윤리라는 위상을 더 부여한다. 왜냐하면 효의 부재는 세상을 병들게 하고 멸망케 하는 직접적인 원인이면서, 동시에 60년 동안 적공(積功)을 한 조상신들과 직접적으로 생명을 준 부모들의 은혜를 저버리는 배은이 개벽시대에는 용납되지 않는다고 보기 때문이다. 이러한 사실들로부터, 대순진리회는 자신의 독특한 사상을 바탕으로 하여 유불도의 전통 효 윤리들을 일부는 수용하고 일부는 재해석과 재창조 과정을 거쳐 윤색해 두고 있음을 살필 수 있다. 즉 대순진리회의 효는 인간 존중의 이념, 구체적으로는 보은상생과 인존(Respect For Man)사상 위에서 정립된 윤리 규정이며, 인격 완성과 도통이라는 종교적 목표에 도달하기 위한 나 자신의 수행이자 복록을 더불어 누리기 위한 부모의 일정한 수행까지 요구하는 개념으로 이해되고, 개벽시대에 구원을 받기 위한 필수 윤리라는 대단히 강화된 종교적 색채를 띠고 있는 것이라고 크게 그려 볼 수 있다는 말이다.