• 제목/요약/키워드: Coliform group

검색결과 188건 처리시간 0.024초

Turmeric (Curcuma longa) Root Powder and Mannanoligosaccharides as Alternatives to Antibiotics in Broiler Chicken Diets

  • Samarasinghe, K.;Wenk, C.;Silva, K.F.S.T.;Gunasekera, J.M.D.M.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제16권10호
    • /
    • pp.1495-1500
    • /
    • 2003
  • Two bio-assays were conducted to evaluate turmeric root powder and mannan-oligosaccharides (MOS) as alternatives to feed antibiotics for broilers. In one trial, one hundred and eighty 19-days old broilers assigned to 18 groups of 10 were fed on one of six experimental diets with three replicates during four weeks. The diets included a basal feed without additives and with either virginiamycin, MOS, or turmeric at 1, 2 and 3 g/kg, respectively. In the second trial, one hundred and forty four 21-days old broilers arranged in 16 groups of nine were fed on the first four diets with four replicates for a similar period. Virginiamycin, MOS and turmeric (1 g/kg) in the first trial generally improved the weight gain of broilers by 3.4, 6.2 and 5.3%, respectively. In the second trial they increased the weight gain significantly (p<0.05) by 8.8, 8.0 and 15.1%, respectively. Additives improved the feed efficiency up to 15.1% and carcass recovery up to 3.1% (p<0.05). Virginiamycin, MOS and turmeric (1 g/kg) markedly reduced the abdominal fat content from 1.91% BW in the control to 1.44, 0.97 and 1.2% BW, respectively, in the first trial. The corresponding values obtained in the second trial were 1.01, 0.55 and 0.6%, respectively as compared to 1.22% in the control group. All additives showed a remarkable inhibition of duodenal coliform bacteria, yeast and mould in the caecum, and all viable microbes in the ileum. A significant (p<0.05) improvement in energy and protein utilization could be recorded with supplemented diets except for high turmeric diets. Dietary 2 and 3 g/kg addition of turmeric reduced energy and protein utilization as well as fat deposition. Present results reveal that turmeric and MOS are satisfactory alternatives to antibiotics in broiler feeds. Both MOS and turmeric possess an antimicrobial effect in vivo. Turmeric may also depress fat deposition in broilers.

Determination of Shelf Life Model of Pork Cutlet and Pork Lard during Accelerated Storage Conditions

  • Park, Jung-Min;Lee, Su-Han;Koh, Jong-Ho;Kim, Jin-Man
    • 한국축산식품학회지
    • /
    • 제38권4호
    • /
    • pp.664-678
    • /
    • 2018
  • This study was carried out to establish shelf life for pork cutlet of ground meat and pork lard by using various quality indicators and to understand how quality changes in these products are accelerated by temperature. The samples were selected and purchased from markets in Korea, and the chosen quality indicators were total aerobic counts and coliform group in microbiological analyses, thiobarbituric acid reactive substances assay, volatile basic nitrogen, pH, acid value, and peroxide value in physical chemical analyses, and sensory evaluation. The pork cutlet samples were stored at $-18^{\circ}C$, $-6^{\circ}C$, and $-1^{\circ}C$, whereas pork lard samples were stored at $10^{\circ}C$, $25^{\circ}C$, $35^{\circ}C$, and $45^{\circ}C$. These temperature conditions were set to real distribution conditions. The samples were then analyzed using various models including of reaction orders, arrhenius equation, and $Q_{10}$ value. The quality limits for each sample were calculated, and shelf life was estimated. The results of this experiment highlighted the importance of temperature control during the distribution process of these products and revealed that temperature is a useful parameter for the establishment of a basic database for shelf life.

냉동 온도에서 소시지의 저장 수명에 관한 연구 (A Study on the Shelf-life of Sausages in Refrigerated Storage)

  • 이용욱;김종규
    • 한국식품위생안전성학회지
    • /
    • 제10권2호
    • /
    • pp.111-131
    • /
    • 1995
  • Quality changes of sausages in refrierated storage for 60 days were investigated. Nine types of sausages produced in Korea were stored at 4$^{\circ}C$(3~5$^{\circ}C$), and then chemical, microbiological, and sensory characteristics were evaluated on every 10 days. The proximate analysis showed considerable variation in fat (23.97%, 17.10~30.20%) with less variation in moisture (51.96%, 48.10~56.30%) and protein (12.96%, 11.40~13.95%), pH value decreased over time averaging from 6.31 to 6.22 with no significant difference. Water activity was consistent over refrigerated storage averaging 0.95. Volatile basic nitrogen (VBN) values were still within 20 mg% though VBN for all types significantly increased over time (p<0.05). Thiobarbituric acid (TBA) values were well below 1.0 though TBA showed significant differences among storage periods (p<0.05). Standard plate counts (SPCs) significantly increased during storage (p<0.05) while coliform group was not counted in all cases. SPCs reached 104 CFU/g in two types after 50 days' storage and were below 105 CFU/g in all types after 60 days' storage. Instrumental texture analysis showed that springiness, adhesiveness, and hardness signficantly decreased over time (p<0.05) while no change was observed in external color. significantly decreased over time the 60-day storage period (p<0.05) while cohesiveness, chewiness and gumminess did not change. Lightness, redness and yellowness of the internal Hunter color significantly decreased over time (p<0.05) while no change was observed in external color. Sensory profile showed that flavor, taste, texture and overall acceptable after 60 days' storage at 4$^{\circ}C$. However, the shelf-life of the samples should be decided in the consideration of the growth rate other spoilage flora coupled with the bacterial growth after 50 days' storage.

  • PDF

서울에서 시판중인 굴의 세균학상 오염과 분리된 비브리오속 균의 항생제 내성 양상 (Antibiotic Resistance of Vibrio spp. and Bacterial Contamination of Commercial Oysters in Seoul, Korea)

  • 홍은경;박소희;윤지희;강병용;하남주
    • Environmental Analysis Health and Toxicology
    • /
    • 제20권4호통권51호
    • /
    • pp.273-277
    • /
    • 2005
  • The purpose of this investigation was to measure the level of bacterial contamination of oysters, and observe antibiotic resistance pattern of Vibro spp. found in oysters. For this experiment, 100 oysters were collected from 100 markets around seoul area from October, 2004 to January, 2005. Bacterial contaminations of the oysters were confirmed by performing the method of standard plate count and desoxycholate lactose agar plate. Total plate counts were $2.7\times$$10^{2}$$\sim$$1.2\times$$10^{5}$ cfu/g. Coliform group were $1.0\times$$1.0^{1}$$\sim$$3.2\times$$10^{5}$ cfu/g. Results have shown that Vibrio spp. was present in $64\%$ of the 100 sampls. Antibiotic resistance pattern of Vibrio spp. showed that $MIC_{50}$ of chloramphenicol was 0.2 g/mL, $MIC_{90}$ was 25 $\mu$g/mL, and $MIC_{50}$ of tetracycline was < 0.05 $\mu$g/mL, $MIC_{90}$ was 25 $\mu$g/mL, and $MIC_{50}$ of ciprofloxacin was 0.01 g/mL, $MIC_{90}$ was 10> $\mu$g/mL. Three of the six strains were identified as Vibrio alginolyricus.

Effects of dietary Antrodia cinnamomea fermented product supplementation on antioxidation, anti-inflammation, and lipid metabolism in broiler chickens

  • Lee, M.T.;Lin, W.C.;Lin, L.J.;Wang, S.Y.;Chang, S.C.;Lee, T.T.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제33권7호
    • /
    • pp.1113-1125
    • /
    • 2020
  • Objective: This study was investigated the effects of dietary supplementation of Antrodia cinnamomea fermented product on modulation of antioxidation, anti-inflammation, and lipid metabolism in broilers. Methods: Functional compounds and in vitro antioxidant capacity were detected in wheat bran (WB) solid-state fermented by Antrodia cinnamomea for 16 days (FAC). In animal experiment, 400 d-old broiler chickens were allotted into 5 groups fed control diet, and control diet replaced with 5% WB, 10% WB, 5% FAC, and 10% FAC respectively. Growth performance, intestinal microflora, serum antioxidant enzymes and fatty acid profiles in pectoral superficial muscle were measured. Results: Pretreatment with hot water extracted fermented product significantly reduced chicken peripheral blood mononuclear cells death induced by lipopolysaccharide and 2,2'-Azobis(2-amidinopropane) dihydrochloride. Birds received 5% and 10% FAC had higher weight gain than WB groups. Cecal coliform and lactic acid bacteria were diminished and increased respectively while diet replaced with FAC. For FAC supplemented groups, superoxide dismutase (SOD) activity increased at 35 days only, with catalase elevated at 21 and 35 day. Regarding serum lipid parameters, 10% FAC replacement significantly reduced triglyceride and low-density lipoprotein level in chickens. For fatty acid composition in pectoral superficial muscle of 35-d-old chickens, 5% and 10% FAC inclusion had birds with significantly lower saturated fatty acids as compared with 10% WB group. Birds on the 5% FAC diet had a higher degree of unsaturation, followed by 10% FAC, control, 5% WB, and 10% WB. Conclusion: In conclusion, desirable intestinal microflora in chickens obtaining FAC may be attributed to the functional metabolites detected in final fermented product. Moreover, antioxidant effects observed in FAC were plausibly exerted in terms of improved antioxidant enzymes activities, increased unsaturated degree of fatty acids in chicken muscle and better weight gain in FAC inclusion groups, indicating that FAC possesses promising favorable mechanisms worthy to be developed.

복숭아의 세척시스템 개발 및 특성 평가 (Characteristics Evaluation and Development of Peach Washing System)

  • 이현석;권기현;정진웅;김병삼;차환수
    • Journal of Biosystems Engineering
    • /
    • 제34권6호
    • /
    • pp.446-453
    • /
    • 2009
  • This study was conducted to find condition of improving the quality of peach by using surfacing washing system. The rate of weight loss of A,B groups were showed slower decreasing trend than CT group and the value of soluble solid degree was not different significantly among all groups from the results of stored peach treat with surface washing system of peach in the first experiment. The colory value of surface were increased with increasing storage period in all treatments. Browning pace of A,B groups were slower than control. Emission of carbon dioxide was increased from the results of respiration rate, 6-10 mL/kg/hr $(10^{\circ}C)$ and 32-41 mL/kg/hr $(25^{\circ}C)$. In second experiment with surface washing system of peach, the value of soluble solid was showed with similar value from 10 $Brix^{\circ}$ to 13 $Brix^{\circ}$. The pace of soft rot of EW groups were lower than CT groups from the results of hardness during storage period. The results of colory value was not showed with significant difference in $15^{\circ}C$ and $20^{\circ}C$ storage temperature but changed to browning in EW groups. The moisture contents was from 85% to 90% in all groups. And the count of total microorganism of EW groups were lower than control. Also total coliform of EW groups were negative. In sensory evaluation, washing peach was showed higher value with significant difference in all acceptability.

저수조 자동 분류를 이용한 효과적인 수질 오염 관리 (Effective Water Pollution Management using Reservoir Tank Automatic Classification)

  • 정경용;전인자
    • 한국콘텐츠학회논문지
    • /
    • 제9권8호
    • /
    • pp.1-8
    • /
    • 2009
  • IT 융합 기술의 발전에 따라 정부의 4대강 복원을 위한 마스터플랜이 구축되면서, 환경 친화적인 수질 오염 관리의 중요성이 부각되고 있다. 본 논문에서는 친환경 저수조의 수질 향상과 온라인 관리를 하기 위해서 저수조 자동 분류를 이용한 효과적인 수질 오염 관리를 제안하였다. 제안된 방법에서는 수질오염 평가의 7가지 요소들을 정의하였고 센서를 이용하여 수소이온농도(pH), 화학적 산소요구량(COD),부유 물질량(SS), 용존 산소량(DO), 대장균군수(MPN), 총인 (T-P), 총질소(T-N)에 따른 적합한 수질 오염 관리를 하였다. 저수조의 7가지의 수질 오염 요소간의 측정치를 평가하고 [1,9] 사이에 분포하도록 정규화하였다. 저수조 자동 분류를 이용한 수질 오염 관리 시스템의 성능 평가를 하기 위해 F-측정식을 이용하여 유용성을 검증하였다. 평가 결과, 기존 시스템에 대한 만족도의 차이가 통계적으로 의미가 있음을 증명하였다.

시판 조미 멍게(Halocynthia roretzi)의 식품학적 품질 특성 (Food Quality and Characterization of Commercial Seasoned Sea Squirt Halocynthia roretzi)

  • 이정석;김민지;이지선;김지혜;김기현;김현정;허민수;김진수
    • 한국수산과학회지
    • /
    • 제46권1호
    • /
    • pp.10-17
    • /
    • 2013
  • This study investigated the food biochemical characterization of commercial seasoned sea squirt Halocynthia roretzi (CSS). The proximate composition of CSS was 77.2-82.7% moisture, 7.1-9.1% crude protein, 0.3-2.6% crude lipid and 3.5-6.3% ash. Taste compound contents of CSS were 2.3-5.4% salinity (saltiness), 0.42-1.12 g/100 g total acidity (sourness) and 114.9-330.2 mg/100 g amino nitrogen (taste intensity). The Hunter color values of CSS were 23.79-32.50 for lightness, 9.97-20.45 for redness, 14.01-20.96 for yellowness and 64.50-76.63 for color difference. The odor intensity of CSS was 35.0-62.0. According to these results, there were large differences in proximate composition, taste compounds, Hunter color values and odor intensity of CSS. Viable cell counts ranged from 6.20 to 7.69 log (CFU/g), and most of the viable cells comprised of lactic acid-forming bacteria. CSS was not detected in the coliform group.

病院廢水의 特性과 處理에 關한 硏究 -消毒劑가 활성스러지법에 미치는 영향- (Studies on the Wastewater of General Hospital and It에s Treatment -Effects of Disinfectants on Activated Sludge Process-)

  • Ra, Kyu Hwan;Ok, Chi Sang
    • 한국환경보건학회지
    • /
    • 제10권1호
    • /
    • pp.13-20
    • /
    • 1984
  • The hospital wastewaters have to be so disposed as to prevent disease and to protect water resources from hazardous substances disinfectants, medicines, and chemicals. Polyvinylpyrrolidone-iodine complex (povidone-iodine) is widely used in the hospital as one of disinfectants. This study was carried out to manifest the effect of disinfectants in growth of activated sludge in treatment of the hospital wastewater by the activated sludge process. The results are as follow. 1. An average water quality of the hospital wastewater showed 7.2 in pH, 3.2 ppm in DO, 293.3 ppm in SS, 96.0 ppm in BOD, 151.1 ppm in COD, 0.4 ppm in povidone-iodine, 0.5 ppm in phenols, 5.4 ppm in surfactants, 1.6 ppm in o-phosphate, 4.6 ppm in $NH_3-N, 249\times 10^4$ counts/100 ml in coliform group organisms, and $1,369\times 10^2$ counts/ml in general counts of bacteria. And wastewater amounts discharged per bed was calculated 70 l/d/bed. 2. In batch culture activated sludge process, each of cresol and povidone-iodine was not effected in less than 0.1 ppm concentration, but the more concentration, the more inhibit the growth rate of activated sludge. In the mixture of two disinfectants, the growth was more inhibited the effect of single disinfectants. So that this reaction is considered as addition effect of two disinfectants. 3. The removal rates of the disinfectants-by continuous culture activated sludge process were 77.6% in 0.4 ppm povidone-iodine, and in BOD was 85.6%. 4. It is desirable that the hospital wastewater is planed in order to be discharged to two system separately, sewer from life system and wastewater from medical system. From those results, it has been concluded that the hospital wastewater has to be treated safely by the activated sludge process.

  • PDF

Effect of Enrobing and Adding Antioxidants on the Quality of Pork Patties

  • Biswas, A.K.;Keshri, R.C.;Kumar, Sunil
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제16권9호
    • /
    • pp.1374-1383
    • /
    • 2003
  • The effect of applying edible coating and addition of antioxidants (butylated hydroxyanisole (BHA) plus butylated hydroxytoluene (BHT) in a 1:1 ratio) on pork patties were investigated prior to achieve desired physico-chemical, microbiological and sensory qualities. For this, five treatments were conducted as (i) control (neither coated nor antioxidants treated); (ii) coated without antioxidants treated; (iii) coated and antioxidants added in the batter mix only (100 ppm); (iv) coated and antioxidants added in meat mix (100 ppm) only; and (v) coated and antioxidants added both in the batter mix (50 ppm) and the meat mix (50 ppm). Addition of antioxidants both in the batter mix and the meat mix significantly (p<0.05) reduced the microbial loads and thiobarbituric acid (TBA) values. The TBA values significantly (p<0.05) increased up to day 14 and then progressively increased with the advancement of each interval of storage days up to 28 days. Total plate count significantly (p<0.05) increased with the increase in storage days.Coliform and Staphylococcus aureus were absent throughout the storage days in all samples. Staphylococcus aureus however, were present in the control group at day 14 and in enrobed (coated) patties (without antioxidants treated) at 28th day. Addition of antioxidants to batter mix and meat mix did not substantially enhance bacteriostatic activity. Application of coatings and antioxidants retarded the loss of firmness, flavor, changes in appearance and color, and also other sensory attributes. Control patties were better with respect to microbial quality and TBA values but had poorer sensory quality than coated patties.