• Title/Summary/Keyword: Cold-adapted live vaccine

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Generation of a cold-adapted PRRSV with a nucleotide substitution in the ORF5 and numerous mutations in the hypervariable region of NSP2

  • Do, Van Tan;Dao, Hoai Thu;Hahn, Tae-Wook
    • Journal of Veterinary Science
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    • v.21 no.6
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    • pp.85.1-85.6
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    • 2020

  • A cold-adapted porcine reproductive and respiratory syndrome virus (CA-VR2332) was generated from the modified live virus strain VR2332. CA-VR2332 showed impaired growth when cultured at 37℃ with numerous mutations (S731F, E819D, G975E, and D1014N) in the hypervariable region of the NSP2, in which the mutation S731F might play a vital role in viral replication at 30℃. Conserved amino acid sequences of the GP5 protein suggests that CA-VR2332 is a promising candidate for producing an effective vaccine against PRRSV infection. Further studies on replication and immunogenicity in vivo are required to evaluate the properties of CA-VR2332.

  • https://doi.org/10.4142/jvs.2020.21.e85 인용 PDF