• The Korean Journal of Physiology
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• v.8 no.2
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• pp.67-74
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• 1974

The object of this research is aimed to determine the activity of adenyl cyclase in both skeletal muscle sarcolemma and fat cell ghost of epididymal adipose tissue isolated from rats exposed to cold for various length of time in an attempt to evaluate whether the tissue sensitivity to catecholamine is increased when rats are exposed to cold for long periods of time Methods: a)Animals: Albino rats ranging in weight from 150 to 200 gm were used throughout this study. For experimental purposes, the rats are divided into two groups: experimental animals were place4 in a cold room at $4^{\circ}C$, controls being kept at $25^{\circ}C$. At the end of 2, 4, 6, 12, and 16 weeks. exposure to cold the rats were used to measure the adenyl cyclase activity. b) Isolation of plasma membrane from skeletal muscle and adipose tissue: The Plasma membrane of skeletal muscle from hind limbs of rats are prepared by the method employed by Rosenthal et at. and fat cell ghost of epididymal adipose tissue of rats by the method employed by Rodbell. c) Adenyl cyclase assay: Adenyl cyclase activity were measured by the method employed by Marinetti et al. Briefly, plasma membrane was incubated with $3^H-ATP$, various amount of noradrenaline and other incubation mixture at $37^{\circ}C$ for 20 minutes. After stopping the enzyme reaction by immersion in boiling water, carrier 3',5'-AMP was added to the system as a marker and $100\;{\mu}1$ aliquots of incubation mixture were pipetted on $20{\time}20$ Whatman No. 3 MM filter paper for one dimensional chromatography. The cyclic AMP spots were cut off and placed in counting vials containing 10ml of Bray's scintillation cocktail. Radioactivity was determined with a Packard Tri-Carb liquid scintillation counter. The enzyme activity is expressed as nanomoles of cyclic AMP produced per mg of membrane per hour. Result: 1. Average adenyl cyclase activity in the plasma membrane of skeletal muscle before and after noradrenaline administration was significantly higher in the cold-exposed rats as compared to the control. Continuous exposure to cold Produced an increased adenyl cyclase activity before and after noradrenaline administration. Adenyl cyclase activity reached peak levels at the 6 weeks exposure to told and level of adenyl cyclase activity remained high. Noradrenaline administration to the incubation medium induced a significant increase in adenyl cyclase activity and the degree of stimulation were proportional to the hormonal concentration But the rate of inclement in adenyl cyclase activity by noradreasline was the same in both groups. 2. Adenyl cyclase activity in fat cell ghost between cold exposed and control rats showed no significant differences before and after noradreualine administration. In summary, it can be concluded that cold adaptation give rise an increased activity of adenyl cyclase in plasma membrane of skeletal muscle in rats.

• The Korean Journal of Food And Nutrition
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• v.31 no.2
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• pp.252-257
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• 2018

It is noted that Dioscorea quinqueloba is a medicinal herb that is widely used to treat cardiovascular disease and is assessed as useful to treat other various medical conditions. The immunopotentiating effects of the protein extract (DQP-1) from Dioscorea quinqueloba were thus formally investigated in vivo under incident of cold stress. In this case study, the spleen and thymus weight in mice was shown to have decreased after a measured exposure to cold stress, while the adrenal gland weight in the mice was shown to have increased. The systematic oral administration of DQP-1 significantly recovered the weight loss of the spleen and suppressed the adrenal gland hypertrophy during the association with cold stress. Additionally, the DQP-1 also restored the ascorbic acid level in the adrenal gland reduced after cold stress. The cold stress exposure lowered the percentage of $CD4^+$ and $CD8^+$ cells in the mouse thymus as determined by the flow cytometric analysis, as well as the levels of some serum immunological cytokines(interleukin-2, interleukin-12, and interferon-${\gamma}$) in the studied mice. The resulting identified weakened immunity caused by cold stress was also recovered by a treatment with DQP-1. The DQP-1 significantly suppressed the formation of serum enzymes of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase, which were systematically elevated during the cold stress episode. These results indicate that DQP-1 can improve immunity in mice that are characteristically weakened under stress.

• The Journal of Internal Korean Medicine
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• v.41 no.4
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• pp.553-562
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• 2020

Objective: To investigate the vasodilatory effect of Sipjeondaebo-tang by inhibiting RhoA activity in vascular cells during cold exposure. Methods: Human vascular endothelial cells and pericytes were pretreated with Sipjeondaebo-tang for 30 min, followed by incubation at 37 ℃ (control) or 25 ℃ (cold exposure) for 30 min. Activation of endothelin-1-mediated RhoA in pericytes was assessed by pretreating the cells with Sipjeondaebo-tang for 30 min, followed by incubation with endothelin-1 at 37 ℃ for 30 min. Western blotting was performed to measure the expression of active RhoA. Endothelin-1 and nitric oxide release from endothelial cells was examined with enzyme-linked immunosorbent assay kits. The formation of stress fibers and focal adhesion complexes was analyzed by immunocytochemistry. Results: Cold treatment activated RhoA in both pericytes and vascular endothelial cells, whereas Sipjeondaebo-tang treatment inhibited this activation. Sipjeondaebo-tang treatment also reversed the cold-mediated production of endothelin-1 and nitric oxide. Cold exposure promoted the formation of stress fibers and focal adhesion complexes by increasing the expression of phospho-focal adhesion complex kinase, whereas Sipjeondaebo-tang treatment suppressed this response. Conclusions: These findings suggested that Sipjeondaebo-tang inhibits cold-induced RhoA activation and its related pathway components, including endothelin-1 and nitric oxide, in vascular cells. Therefore, Sipjeondaebo-tang could be beneficial for the treatment of Raynaud's phenomenon.

• Journal of Plant Biotechnology
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• v.50
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• pp.225-231
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• 2023

Cold stress is one of the most vulnerable environmental stresses that affect plant growth and crop yields. With the recent advancements in genetic approaches using Arabidopsis and other model systems, genes involved in cold-stress response have been identified and the key cold signaling factors have been characterized. Exposure to low-temperature stress triggers the activation of a set of genes known as cold regulatory (COR) genes. This activation process plays a crucial role in enhancing the resistance of plants to cold and freezing stress. The inducer of the C-repeatbinding factor (CBF) expression 1-CBF module (ICE1-CBF module) is a key cold signaling pathway regulator that enhances the expression of downstream COR genes; however, this signaling module in Panax ginseng remains elusive. Here, we identified cold-signaling-related genes, PgCBF1, PgCBF3, and PgICE1 and conducted functional genomic analysis with a heterologous system. We confirmed that the overexpression of cold- PgCBF3 in the cbf1/2/3 triple Arabidopsis mutant compensated for the cold stress-induced deficiency of COR15A and salt-stress tolerance. In addition, nuclearlocalized PgICE1 has evolutionarily conserved phosphorylation sites that are modulated by brassinsteroid insensitive 2 (PgBIN2) and sucrose non-fermenting 1 (SNF1)-related protein kinase 3 (PgSnRK3), with which it physically interacted in a yeast two-hybrid assay. Overall, our data reveal that the regulators identified in our study, PgICE1 and PgCBFs, are evolutionarily conserved in the P. ginseng genome and are functionally involved in cold and abiotic stress responses.

• Journal of the Korean Society of Food Science and Nutrition
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• v.15 no.1
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• pp.9-14
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• 1986

Fuji and Jonathan apples were stored at cold room and at CA storage conditions of $3%O_2-2%CO_2$ for Jonathan and $4%O_2-3%CO_2$ for Fuji for 8 months, and then tranferred to air at $20^{\circ}C$, 70%RH to compare the some factors related to texture of the apples stored at different storage conditions during exposure at $20^{\circ}C$. Total pectin content and firmness of the apples reduced, considerably with concomitant increases of water-soluble pectin content and polygalacturonase activity during exposure at $20^{\circ}C$ after storage. And the changes were much more serious in the first 5 days than any other period and in the apples stored at cold room, especially in Jonathan than the apples stored at CA storage conditions. From the sensory test the apples stored at cold room maintained acceptable quality for 5days after removal from storage condition. The acceptability from the test was lenghtened for more than 5 days in Jonathan and more than 10 days for Fuji stored at CA storage conditions. when compared with those of the apples stored at cold room.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.33 no.3
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• pp.317-331
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• 2023

Objectives: Construction workers are exposed to various hazardous substances simultaneously. However, little is known about the exposure hazards in construction industry. This study was aimed at identifying the risk of exposure hazards among construction workers. Methods: The expert survey (n=29) was conducted, including construction industry health managers (n=11) and work environment monitoring experts (n=18), on exposure probability, intensity and risk of hazardous substances by construction occupations Results: The exposure hazards of 30 construction occupations were identified and summarized through a literature review and expert survey. The most prevalent hazards were in order of noise, awkward posture, heat/cold, crystalline silica, cement/concrete dust, metal fumes, and volatile organic compounds. The hazards with highest risk score(over seven points) at construction occupations were noise(formwork carpenter, concrete finisher, rebar worker, demolition worker, driller/rock blaster), hazardous rays(welder), heat/cold (earthworks, formwork carpenter, rebar worker, concrete placer, scaffolder), awkward posture(bricklayer, caulker/tile setter, rebar worker) and heavy lifting(bricklayer, rebar worker). Among construction workers, the job types with the highest risk of exposure to carcinogens, and in which occupational cancer has been reported, were in order of stonemason, concrete finisher, rock blaster, welder, insulation installer, painter, scaffolder, plant worker and earthworks in order Conclusions: Systematic research and discussion on occupational disease among construction workers and its various hazardous factors are needed to establish job exposure matrix for facilitating standard for promptly processing the workers' compensation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.1
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• pp.13-16
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• 2003

Seeds of burcucumber were treated with accelerated aging, cold-stratification, and light quality illuminated during desiccation to enhance their germination and seedling emergence. The germination was increased by aging and cold-stratification although the latter treatment showed greater effectiveness than the former one. In the combined treatment of aging 6 days at $45^{\circ}C$ and cold-stratification, the germination was promoted under longer period of cold-stratification to reach nearly 100% in 3 week cold-stratification on the ninth day from sowing. In the sequentially combined treatment of aging, cold-stratification, and light quality during 24 hour desiccation at $35^{\circ}C$, no-stratified seeds showed the highest rate in red light treatment but the lowest in far-red light. This implies that the phytochrome action run during the desiccation of imbibed seeds. The red light exposure during drying for the cold-stratified seeds after aging accelerated the germination even more than the dark treatment and germinated 100% on the next day of sowing. It is concluded that the sequential treatment of aging, cold-stratification, and red light illumination during desiccation can highly promote percentage and speed of burcucumber seed germination.

• Journal of the Korean Society of Clothing and Textiles
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• v.21 no.6
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• pp.977-987
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• 1997

The present study was designed to see what the local cooling of different body regions especially head and neck, hands and feet effect physiological responses in cold environment. Four male subjects wore garments covering whole body except face and rested for 20 min and then they rested for 40 min with uncovered head, neck, hands and feet, respectively in a cold environment(10$\pm$1$^{\circ}C$, 50$\pm$5%R.H.) 1. Rectal temperature increased when hands and feet were exposed to cold environment respectively, and when head and neck, hands and feet were exposed to cold environment together. 2. Exposed skin temperatures fell in cold environment. And hands temperature was lower than any other exposed skin temperatures. The hands temperature was significantly lower when head was exposed than when head was covered. And the feet temperature were significantly lower when hands were exposed than when hands were covered. 3. Mean skin temperature was the lowest when head and neck, hands and feet were exposed simultaneously, In conclusion, skin temperatures of extremities were decreased due to exposure to the cold environment. Especially upper extremities were lower than lower extremities by exposed parts of the body. It seemed that the extremities played the role of cold receptors but head and neck didn't. And there were large heat losses from the unprotected head and neck. In cold environment of 1$0^{\circ}C$ , thus, it is suggested for the purpose of thermoregulatory responses that head and neck would be covered and extremities would be exposed, especially upper extremities.

• Journal of the Korean Society of Clothing and Textiles
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• v.35 no.7
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• pp.713-720
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• 2011

This study investigated the effect of cold acclimatization training on body composition including weight, fat mass, and muscle mass with 10 subjects (5 males and 5 females). During the 3-week acclimatization training program, they visited an artificial climate chamber ($15^{\circ}C$) 15 times and were exposed to cold environment with light clothing for 2 hours. Body composition was measured before and after cold training using bioelectric impedance analysis that was later compared by a paired t-test. In the process of thermoregulation, muscle contraction was accompanied by increased substrate metabolism for rising heat production. After cold training, the muscle mass increased and fat mass decreased significantly (p<.1, p<.05), subsequently the body composition changed. It was found that cold acclimatization training could be used as a treatment for obesity. It was suggested that further investigation on the long term effects of mild cold exposure using clothing and its potential applicability as an obesity treatment.

• Animal cells and systems
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• v.7 no.1
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• pp.29-33
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• 2003

To understand the adaptational strategy of Korean chipmunks (Tamias sibiricus) to cold temperature, blood and serum properties, and thyroid gland activity of cold-exposed chipmunks were examined. The number of erythrocytes and hemoglobin concentration increased, but platelets decreased in cold-exposed chipmunks compared with warm chipmunks. Serum total protein levels increased at early phase of cold-exposure, and decreased thereafter. Plasma glucose levels showed a transitory increase in cold temperature. Although there was significant decrease in serum total thyroxine level in cold-exposed chipmunks, serum total triiodothyronine level changed little. Histological analysis of thyroid glands demonstrated decreased thyroid activity, suggesting that differences in the blood and serum properties between the warm and cold-exposed chipmunks may be due to the different metabolic strategy associated with cold temperature.