• Journal of Applied Biological Chemistry
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• v.60 no.3
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• pp.257-263
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• 2017

A cellulolytic strain Y2 was isolated from soil obtained in the Canadian Alpine region. The isolate was identified as Paenibacillus sp. Y2 by 16S rRNA sequencing. When grown in LB medium supplemented with carboxymethyl-cellulose (CMC), CMCase production increased to 122.0% of that observed in LB without CMC. Culture supernatant was concentrated by ultrafiltration and 80% ammonium sulfate precipitates were separated by Hi-Trap Q and CHT-II chromatography. The purified enzyme (EG-PY2) showed a homogeneous single band and the molecular mass was estimated to be 38 kDa by SDS-PAGE. Optimum pH and temperature of the enzyme were 4.5 and $30^{\circ}C$, respectively. The half-life of enzyme activity at 50 was 140.7 min, but the enzyme was drastically inactivated within 5 min at $55^{\circ}C$. The enzyme was highly activated to 135.7 and 126.7% by 5.0 mM of $Cu^{2+}$ or $Mg^{2+}$ ions, respectively, and moderately activated by $Ba^{2+}$ and $Ca^{2+}$ ions, whereas it was inhibited to 76.8% by $Fe^{2+}$, and to ${\leq}50%$ by $Mn^{2+}$, $Co^{2+}$, $Zn^{2+}$, and EDTA. The enzyme was activated to 211.5% in the presence of 0.5 M of NaCl and greatly tolerant to 3.15M of NaCl. The enzyme showed 2.98 times higher ${\beta}$-glucanase activity than CMCase activity. Based on these results, it can be concluded that EG-PY2 is an acidophilic, cold-active, and halotolerant endoglucanase. The authors suggest it is considered to be useful for various industrial applications, such as, fruit juice clarification, acidic deinking processes, high-salt food processing, textile and pulp industries, and for biofuel production from seaweeds.

• Animal Systematics, Evolution and Diversity
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• v.30 no.1
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• pp.9-15
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• 2014

Pagurus undosus and Pagurus parvispina, collected from the East Sea by fishery trap, are newly recorded from Korean waters. Pagurus undosus is distinguished from other hermit crab by its right cheliped slightly elevated medially, and with broad ridge formed by deep depressions mesial and lateral to midline. Pagurus parvispina is distinguished from other hermit crab by its right cheliped covered with large spine and long tufts of setae. These species live in cold water areas and their geographical distribution is extended southwardly by the present study. A specimen of the former species, P. undosus, was found living in a shelter formed by a sponge, similar to that observed in Pagurus pectinatus. Descriptions and figures of these two species are provided in this paper. Currently, 27 species of the genus Pagurus are recorded in Korean fauna.

• Journal of the Optical Society of Korea
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• v.5 no.4
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• pp.131-135
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• 2001

We report experiemtnal results demonstrating the electromagnetically induced transparency (EIT) in trapped Cs atoms. EIT occurs at the Λ-type configuration where the re0-pumping laser simultaneously plays a role as the coupling laser in the presence of a magneto-optical trapping and weak magnetic fields. Dependences of EIT signal on both the intensity and the detuning of the coupling laser were investigated. Linear absorption spectra for cold cesium atoms in the magneto-optical trap have been observed and shown the pronounced dispersion-like structure with sub-natural linewidth of 1 MHz due to the cooling laser.

• Applied Biological Chemistry
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• v.35 no.1
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• pp.36-41
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• 1992

Several commercial cottonseed, corn and rapeseed oils were stored at $60^{\circ}C\;and\;70^{\circ}C$ with daily exposure of fluorescent light for 12 hours and evaluated their rancidity by headspace gas chromatographic analysis of pentanal and hexanal. The data of gas chromatographic analysis was compared with organoleptic flavor evaluation. For headspace gas chromatographic analysis, the volatile compounds were recovered by porous polymer trap and flushed into a fused silica capillary column at $250^{\circ}C$. Twenty-three GC peaks were identified on the basis of relative retention time of reference compounds and gas chromatography-mass spectrometry. The results showed that the contents of pentanal and hexanal were linearly increased during storage. A very simple linear relationship was found between organoleptic flavor scores and amounts of two volatile compounds with very high correlation coefficient. This results suggested the possible implication of pentanal and hexanal as an quality index for rancidity evaluation of cottonseed, corn and rapeseed oils.

• 한국신재생에너지학회:학술대회논문집
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• 2009.06a
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• pp.361-364
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• 2009

KEPRI has studied planar type SOFC stacks using anode-supported single cells and kW class co-generation systems for residential power generation. A 1kW class SOFC system consisted of a hot box part, a cold BOP part and a water reservoir. A hot box part contains a SOFC stack made up of 48 cells with $10{\times}10cm^2$ area and ferritic stainless steel interconnectors, a fuel reformer, a catalytic combustor and heat exchangers. Thermal management and insulation system were especially designed for self-sustainable operation. A cold BOP part was composed of blowers, pumps, a water trap and system control units. When a 1kW class SOFC system was operated at $750^{\circ}C$ with hydrogen, the stack power was 1.2kW at 30 A and 1.6kW at 50A. Turning off an electric furnace, the SOFC system was operated using hydrogen and city gas without any external heat source. Under self-sustainable operation conditions, the stack power was about 1.3kW with hydrogen and 1.2kW with city gas respectively. The system also recuperated heat of about 1.1kW by making hot water. Recently KEPRI developed stacks using $15{\times}15cm^2$ cells and tested them. KEPRI will develop a 5 kW class CHP system using $15{\times}15cm^2$ stacks by 2010.

• Transactions of the Korean hydrogen and new energy society
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• v.20 no.5
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• pp.384-389
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• 2009

KEPRI (Korea Electric Power Research Institute) has studied planar type solid oxide fuel cell (SOFC) stacks using anode-supported cells and kW class co-generation systems for residential power generation. In this work, a 1 kW SOFC system consisted of a hot box part, a cold BOP (balance of plant) part, and a hot water reservoir. The hot box part contained a SOFC stack made up of 48 cells, a fuel reformer, a catalytic combustor, and heat exchangers. Thermal management and insulation system were especially designed for self-sustainable operation in that system. A cold BOP part was composed of blowers, pumps, a water trap, and system control units. When the 1 kW SOFC stack was tested using hydrogen at $750^{\circ}C$, the stack power was about $1.2\;kW_e$ at 30 A and $1.6\;kW_e$ at 50 A. Turning off an electric furnace, the SOFC system was operated using hydrogen and city gas without any external heat source. Under self-sustainable operation conditions, the stack power was about $1.3\;kW_e$ with hydrogen and $1.2\;kW_e$ with city gas respectively. The system also recuperated heat of about $1.1\;kW_{th}$ by making hot water.

• Korean journal of applied entomology
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• v.54 no.4
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• pp.303-310
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• 2015

The diamondback moth, Plutella xylostella, overwinters in some protected areas in Korea. Using a sex pheromone trap, the adults were monitored since the occurrence of the overwintering populations. In Andong, P. xylostella exhibited four adult peaks in a year. Biological characters, such as cold tolerance, insecticide susceptibility, and developmental rate, were analyzed and showed a significant variation among different local overwintering populations. Population genetic variation was assessed with molecular markers, in which the initial high genetic variation among the overwintering populations decreased with the progress of seasons. These results suggests that there may be a significant migration of P. xylostella to decrease the genetic variation among the different local populations that are different in biological characters.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.5
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• pp.82-90
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• 2020

Due to the complicated shape of the spider, the production method was changed from cold to warm forging. Finite element analysis was performed to predict the forging load and shape using the enclosed hydraulic die set. As the forging load increases due to the spider die volume, die stress analyses were performed to optimize the die design in order to reduce the die stress in various conditions. Large deformation while producing the complicated forging parts induces high forging load, which is one of the main parameters of the forging surface defects. The forging process was analyzed to find out the root cause of the surface defects generated during the spider production for various parameters, thereby revealing that the radius of die in the defect zone influenced the air trap depth, being the root cause of the surface defect. It was verified that die life was increased and the surface defect was eliminated by changing the die design during the mass production test.

• Nuclear Engineering and Technology
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• v.24 no.2
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• pp.141-153
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• 1992

Excess tritium analysis was peformed to verify whether or not cold fusion occurs during electrolysis of heavy water in the current density range of 83~600 mA/$\textrm{cm}^2$ for a period of 24 ~ 48 hours with use of palladium electrodes of seven different processing treatments and geometries. The extent of recombination of D$_2$ and $O_2$gases in the electrolytic cell was measured for the calculation of accurate enthaplpy values. The behavior and interaction of hydrogen atoms with defects in Pd electrodes were examined using the Sieverts gas charging and the positron annihilation(PA) method. Slight enrichment of tritium observed was attributed to electrolytic enrichment but not to the formation of a by-product of cold fusion. The extent of recombination of D$_2$and $O_2$gases was 32%. Hence the excess heat measured during the electrolysis was considered to be due to the exothermic reaction of recombination but not to nuclear fusion. Lifetime results from the PA measurements on the Pd electrodes indicated that hydrogen atoms could be trapped at dislocations and vacancies in the electrodes and that dislocations were slightly more preferred sites than vacancies. It was also inferred from R parameters that the formation of hydrides was accompanied by generation of mostly dislocations. Doppler broadening results of the Pd electrodes indicated that lattiec defect sites where positrons were trapped first increased and then decreased, and this cycle was repeated as electrolysis continued. It can be inferred from PA measurements on the cold-rolled Pd and the isochronally annealed Pd hydride specimens that microvoid-type defects existed in the hydrogen-charged electrode specimen.

• Proceedings of the Korean Vacuum Society Conference
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• 1999.07a
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• pp.190-190
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• 1999

박막 표면에 대한 경원소 분석법인 탄성 되튐 반도법을 개발하여 수소, 탄소, 질소등 분석에 이용하고 있다. 이때 입사 입자로 Cl 9.6MeV를 이용하였는데, 표적 표면에 탄소막이 흡착되는 현상을 발견하였다. cold trap 및 cold finger를 사용하여 진공도를 개선하므로서, 탄소막 흡착의 한 원인으로 알려져 잇는 chamber 주변의 진공도 변화를 시켜보았다. 하지만 전혀 탄소막이 생기지 않는 10-10torr 이하 진공을 만드는 것은 많은 비용과 장비를 필요로 하는 상당히 힘든 작업이어서, 이차적으로 탄소막이 표적 표면에 달라 붙게 하는 원인으로 추정되는 이차 전자의 발생을 고에너지 이온빔으로 조사하였다. 일반적으로 이차전자의 발생은 이온빔과 표적과의 충돌에 의한 고체 표면으로부터의 전자방출 현상으로 오래전부터 연구되어져 왔다. 여기에는 두가지 다른 구조가 존재하는 것으로 알려져 있다. 그 중 하나는 입사 입자의 전하와 표적 표면사이 작용하는 potential 에너지가 표적 표면의 일함수(재가 function) 보다 클 때에 일어나는 potential emission이다. 즉 표적 궤도에 존재하는 전자와 입사 이온빔 사이의 potential 이 표적의 전자를 들뜨게 만들고, 이 potential의 크기가표적의 표면 장벽 potential 보다 충분히 클 뜸 전자가 방출하는 현상을 말한다. 다른 또 하나의 방출구조로는 입사 이온이 표적 표면의 원자와의 충돌에 의해 직접저인 에너지 전달을 통한 전자 방출을 말하는데, 이를 kienetic emission(이하 KE)이라 한다. 본 연구에서는 Tandem Van de graaff 가속기로 고에너지 이온빔을 만들어 Au에 충돌시키므로서 kinetic emission을 통하여 Au에서 발생한는 이차전자의 방출 수율 및 에너지를 측정하였다.장구조로 전체 성장 양식을 예견할 수 있다. 일반적인 경향은 Ep가 커질수록 fractal 성장형태가 되며, Ed가 적을수록 cluster 밀도가 작아지나, 같은 Ed+Ep에 대해서는 동일한 크기의 팔 넓이(수평 수직 방향 cluster 두께)를 가진다. 따라서 실험으로부터 얻은 cluster의 팔 넓이로부터 Ed+Ep 값을 결정할 수 있고, cluster 밀도와 fractal 차원으로부터 각각 Ed와 Ep값을 분리하여 얻을 수 있다. 또한 다층 성장에 대한 거칠기(roughness) 값으로부터 Es값도 구할 수 있다. 양방향 대칭성을 갖지 않은 fcc(110) 표면과 같은 경우, 형태는 다양하지만 동일한 방법으로 추정이 가능하다. (110) 표면의 경우 nearest neighbor 원자가 한 축으로 형성되고 따라서 이 축과 이것과 수직인 축에 대한 상호작용이나 분산 장벽 모두가 비대칭적이다. 따라서 분산 장벽도 x-축, y-축 방향에 따라 분리하여 Edx, E요, Epx, Epy 등과 같이 방향에 따라 다르게 고려해야 한다. 이러한 비대칭적인 분산 장벽을 고려하여 KMC 시뮬레이션을 수행하면 수평축과 수직축의 분산 장벽의 비에 따라 cluster의 두께비가 달라지는 성장을 볼 수 있었고, 한 축 방향으로의 팔 넓이는 fcc(100) 표면의 경우 동일한 Ed+Ep값에 대응하는 팔 넓이와 거의 동일한 결과가 나타나는 것을 볼 수 있다. 따라서 이러한 비대칭적인 모양을 가지는 성장의 경우도 cluster 밀도, cluster 모양, cluster의 양 축 방향 길이 비, 양 축 방향의 평균 팔 넓이로부터 각 축 방향의 분산 장벽을 얻어낼 수 있을 것으로 보인다. 기대할 수 있는 여러 장점들을 보고하고자 한다.성이 우수한 시편일수록 grain의 크기가 큰 것으로 나타났고 결정성이 우수한 시편의 경우에서는 XR