• Title/Summary/Keyword: Coding control

Search Result 743, Processing Time 0.026 seconds

Dental-derived cells for regenerative medicine: stem cells, cell reprogramming, and transdifferentiation

  • Young-Dan Cho;Kyoung-Hwa Kim;Yong-Moo Lee;Young Ku;Yang-Jo Seol
    • Journal of Periodontal and Implant Science
    • /
    • v.52 no.6
    • /
    • pp.437-454
    • /
    • 2022
  • Embryonic stem cells have been a popular research topic in regenerative medicine owing to their pluripotency and applicability. However, due to the difficulty in harvesting them and their low yield efficiency, advanced cell reprogramming technology has been introduced as an alternative. Dental stem cells have entered the spotlight due to their regenerative potential and their ability to be obtained from biological waste generated after dental treatment. Cell reprogramming, a process of reverting mature somatic cells into stem cells, and transdifferentiation, a direct conversion between different cell types without induction of a pluripotent state, have helped overcome the shortcomings of stem cells and raised interest in their regenerative potential. Furthermore, the potential of these cells to return to their original cell types due to their epigenetic memory has reinforced the need to control the epigenetic background for successful management of cellular differentiation. Herein, we discuss all available sources of dental stem cells, the procedures used to obtain these cells, and their ability to differentiate into the desired cells. We also introduce the concepts of cell reprogramming and transdifferentiation in terms of genetics and epigenetics, including DNA methylation, histone modification, and non-coding RNA. Finally, we discuss a novel therapeutic avenue for using dental-derived cells as stem cells, and explain cell reprogramming and transdifferentiation, which are used in regenerative medicine and tissue engineering.

Cloning, cSNP Identification, and Genotyping of Pig Complement Factor B(CFB) Gene Located on the SLA Class III Region (SLA Class III 영역의 돼지 Complement Factor B(CFB) 유전자의 Cloning, cSNP 동정 및 유전자형 분석)

  • Kim, Jae-Hwan;Lim, Hyun-Tae;Seo, Bo-Yeong;Zhong, Tao;Yoo, Chae-Kyoung;Jung, Eun-Ji;Jeon, Jin-Tae
    • Journal of Animal Science and Technology
    • /
    • v.50 no.6
    • /
    • pp.753-762
    • /
    • 2008
  • The primers for RT-PCR and RACE-PCR were designed by aligning the pig genomic sequence and the human complement factor B(CFB) coding sequence(CDS) from the GenBank. Each PCR product was amplified in pig cDNA and sequencing was carried out. The CDS length of pig CFB gene was determined to be 2298 bp. In addition, the pig CDS was more longer than human and mouse orthologs because of insertion and deletion. The identities of porcine nucleotide sequences with those of human and mice were 84% and 80%, and the identities of amino acids were 79% to 77%, respectively. Three complement control protein(CCP) domains, one Von Willebrand factor A(VWFA) domain and a serine protease domain, that are revealed typically in mammals, were found in the pig CFB gene. Based on the CDSs determined, the primers were designed in intron regions for amplification of entire length of exons. In amplification and direct sequencing with genomic DNAs of six pig breeds, three cSNPs(coding single nucleotide polymorphisms) were identified and verified as missense mutations. Using the Multiplex-ARMS method, we genotyped and verified the mutations identified from direct sequencing. To demonstrate recrudescence, we performed both direct sequencing and Multiplex-ARMS with two randomly selected DNA samples. The genotype of each sample exhibited the same results using both methods. Therefore, three cSNPs were identified from pig CFB gene and that can be used for haplotype analysis of the swine leukocyte antigen(SLA) class III region. Moreover, the results indicate that the Multiplex-ARMS method should be powerful for genotyping of genes in the SLA region.

Dual Codec Based Joint Bit Rate Control Scheme for Terrestrial Stereoscopic 3DTV Broadcast (지상파 스테레오스코픽 3DTV 방송을 위한 이종 부호화기 기반 합동 비트율 제어 연구)

  • Chang, Yong-Jun;Kim, Mun-Churl
    • Journal of Broadcast Engineering
    • /
    • v.16 no.2
    • /
    • pp.216-225
    • /
    • 2011
  • Following the proliferation of three-dimensional video contents and displays, many terrestrial broadcasting companies have been preparing for stereoscopic 3DTV service. In terrestrial stereoscopic broadcast, it is a difficult task to code and transmit two video sequences while sustaining as high quality as 2DTV broadcast due to the limited bandwidth defined by the existing digital TV standards such as ATSC. Thus, a terrestrial 3DTV broadcasting with a heterogeneous video codec system, where the left image and right images are based on MPEG-2 and H.264/AVC, respectively, is considered in order to achieve both high quality broadcasting service and compatibility for the existing 2DTV viewers. Without significant change in the current terrestrial broadcasting systems, we propose a joint rate control scheme for stereoscopic 3DTV service based on the heterogeneous dual codec systems. The proposed joint rate control scheme applies to the MPEG-2 encoder a quadratic rate-quantization model which is adopted in the H.264/AVC. Then the controller is designed for the sum of the left and right bitstreams to meet the bandwidth requirement of broadcasting standards while the sum of image distortions is minimized by adjusting quantization parameter obtained from the proposed optimization scheme. Besides, we consider a condition on maintaining quality difference between the left and right images around a desired level in the optimization in order to mitigate negative effects on human visual system. Experimental results demonstrate that the proposed bit rate control scheme outperforms the rate control method where each video coding standard uses its own bit rate control algorithm independently in terms of the increase in PSNR by 2.02%, the decrease in the average absolute quality difference by 77.6% and the reduction in the variance of the quality difference by 74.38%.

Chronic pain control in patients with rheumatoid arthritis (만성통증 환자의 통증 조절)

  • Eun, Young
    • Journal of muscle and joint health
    • /
    • v.2 no.1
    • /
    • pp.17-40
    • /
    • 1995
  • Rheumatoid arthritis is the one of the chronic diseases, one of its major symptoms is a chronic pain. Despite developing medical treatment and surgical techniques, it is suggested that to control the pain is the goal of the treatment. But pain is an inner experience and even those closest to the patient cannot truly observe its progress or share in its suffering. The National Academy of Sciences Institute of Medicine's report on Pain and Disability concluded that there is no objective measure of pain-(exactly) no pain thermometer-nor can there ever be one, because the experience of pain is inseparable from personal perception and social influence such as culture. To explore chronic pain experience is to understand the process and property of the patient's perception of pain through the response to pain, the coping with pain, and the adaptation to pain. Therefore a qualitative study was conducted in order to gain an understanding of pain experience of patients with RA in korea. I used naturalistic inquiry as a research methodology, which had 5 axioms, the first is that realities are multiple, constructed, and holistic, the second is that knower and known are interactive, inseparable, the third is only time and context bound working hypotheses(idiographic statements) are possible, the forth is all entities are in a state of mutual simultaneous shaping, so that it is impossible to distinguish causes from effects and the last is that inquiry is value-bound. Purposive sampling was conducted as a sampling. 20 subjects who experienced pain over 10 years, lived in middle-sized city and big city in Korea, and 17 women and 3 men. The subject's age was from 32 to 62 (average 48.8), all were married, living with their spouse and children, except two-one divorced and the other widow before they became ill. I collected data using In depth structured interview. I had interviews two or three times with each subject, and the interviews were conducted at each subject's home. Each interview lasted about two hours an average. A recording was taken with the consent of the subject. I used inductive data analysis-such as unitizing and categorizing. unitizing is a process of coding, whereby raw data are systematically transformed and aggregated into units. Categorizing is a process wherby previously unitized data are organized into categories that provide descriptive or inferential information about the context or setting from which the units were derived. This process is used constant comparative method. The pain controlling process is composed of behavior of pain control. The behaviors of pain control are rearranging of ADL, hiddening role conflict, balancing treatment, and changing social relation. Rearranging of ADL includes diet management, sleep management, and the adjustment of daily life activities. The subjects try to rearrange their daily activities by modified style of motions, rearranging time span & range of activities, using auxillary facilities, and getting help in order to keep on the pace of daily life. Hiddening role conflict means to reduce conflicts between sick role and their role as a family member. In this process, the subjects use two modes, one is to control the pain complaints, and the other is to internalize the value which is to stay home is good for caring her children and being a good mother. To control pain complaints is done by 'enduring', 'understanding' the other family members, or making them undersood in order to reduce pain. Balancing treatment is composed of two aspects. One is to keep the pain within the endurable level, the other is to keep in touch with medical personnel in order to get the information of treatment and emotional support. Changing social relation is made by information seeking and sharing, formation of mutual support relation, and finally simplification of social relationships. The subjects simplify their social relationships by refraining from relations with someone who makes them physically and psychologically strained. In particular the subjects are apt to avoid contact with in-laws, and the change of relation to in-laws results in lessening the family boundary. In the course of this process, they confront the crisis of family confict result in family dissolution. This crisis is related to the threat of self-existence. Findings from this study contribute to understanding the chronic pain experience. To advance this study, we should compare this result with other cases in different cultural contexts. I think to interpret these results, korean cultural background should be considered. Especially the different family concept, more broader family members and kinship network, and the traditional medical knowledge influences patients' behavior.

  • PDF

Selection of Cross-layered Retransmission Schemes based on Service Characteristics (서비스 특성을 고려한 다 계층 재전송 방식 선택)

  • Go, Kwang-Chun;Kim, Jae-Hyun
    • Journal of the Institute of Electronics and Information Engineers
    • /
    • v.52 no.5
    • /
    • pp.3-9
    • /
    • 2015
  • The wireless communication system adopts an appropriate retransmission scheme on each system protocol layer to improve reliability of data transmission. In each system protocol layer, the retransmission scheme operates in independently other layers and operates based on the parameters without reference to end-to-end performance of wireless communication system. For this reason, it is difficult to design the optimal system parameters that satisfy the QoS requirements for each service class. Thus, the performance analysis of wireless communication system is needed to design the optimal system parameters according to the end-to-end QoS requirements for each service class. In this paper, we derive the mathematical model to formulate the end-to-end performance of wireless communication system. We also evaluate the performance at the MAC and transport layers in terms of average spectral efficiency and average transmission delay. Based on the results of performance evaluations, we design the optimal system parameters according to the QoS requirements of service classes. From the results, the HARQ combined with AMC is appropriate for the delay-sensitive service and the ARQ combined with AMC is appropriate for a service that is insensitive to transmission delay. Also, the TCP can be applied for the delay-insensitive service only.

A Design of Key Generation and Communication for Device Access Control based on Smart Health Care (스마트 헬스케어 기반의 디바이스 접근제어를 위한 키 생성 및 통신기법 설계)

  • Min, So-Yeon;Lee, Kwang-Hyong;Jin, Byung-Wook
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.17 no.11
    • /
    • pp.746-754
    • /
    • 2016
  • Smart healthcare systems, a convergent industry based on information and communications technologies (ICT), has emerged from personal health management to remote medical treatment as a distinguished industry. The smart healthcare environment provides technology to deliver vital information, such as pulse rate, body temperature, health status, and so on, from wearable devices to the hospital network where the physician is located. However, since it deals with the patient's personal medical information, there is a security issue for personal information management, and the system may be vulnerable to cyber-attacks in wireless networks. Therefore, this study focuses on a key-development and device-management system to generate keys in the smart environment to safely manage devices. The protocol is designed to provide safe communications with the generated key and to manage the devices, as well as the generated key. The security level is analyzed against attack methods that may occur in a healthcare environment, and it was compared with existing key methods and coding capabilities. In the performance evaluation, we analyze the security against attacks occurring in a smart healthcare environment, and the security and efficiency of the existing key encryption method, and we confirmed an improvement of about 15%, compared to the existing cipher systems.

An Effective Method to Treat The Boundary Pixels for Image Compression with DWT (DWT를 이용한 영상압축을 위한 경계화소의 효과적인 처리방법)

  • 서영호;김종현;김대경;유지상;김동욱
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.27 no.6A
    • /
    • pp.618-627
    • /
    • 2002
  • In processing images using 2 dimensional Discrete Wavelet Transform(2D-DWT), the method to process the pixels around the image boundary may affect the quality of image and the cost to implement in hardware and software. This paper proposed an effective method to treat the boundary pixels, which is apt to implement in hardware and software without losing the quality of the image costly. This method processes the 2-D image as 1-D array so that 2-D DWT is performed by considering the image with the serial-sequential data structure (Serial-Sequential Processing). To show the performance and easiness in implementation of the proposed method, an image compression codec which compresses image and reconstructs it has been implemented and experimented. It included log-scale fried quantizer, but the entropy coder was not implemented. From the experimental results, the proposed method showed the SNR of almost the same SNR(Signal to Noise Ratio) to the Periodic Expansion(PE) method when the compression ratio(excluding entropy coding) of 2:1, 15.3% higher than Symmetric Expansion(SE) method, and 9.3% higher than 0-pixel Padding Expansion(ZPE) method. Also PE method needed 12.99% more memory space than the proposed method. By considering only the compression process, SE and ZPE methods needed additional operations than the proposed one. In hardware implementation, the proposed method in this paper had 5.92% of overall circuit as the control circuit, while SE, PE, and ZPE method has 22%, 21,2%, and 11.9% as the control circuit, respectively. Consequently, the proposed method can be thought more effective in implementing software and hardware without losing any image quality in the usual image processing applications.

Genetic Screening of the Canine Transcription Factor AP-2 Beta(TFAP2B) Gene in Dogs with Patent Ductus Arteriosus(PDA) (동맥관 개존증(PDA)에 이환된 개에서의 전사 인자 AP-2 beta(TFAP2B) 유전자 스크리닝)

  • Nam, So-Jeong;Hyun, Chang-Baig
    • Journal of Veterinary Clinics
    • /
    • v.26 no.2
    • /
    • pp.123-129
    • /
    • 2009
  • Patent ductus arteriosus(PDA) is an abnormal shunt between the descending aorta and pulmonary artery through the incompletely closed ductus arteriosus and is the most common congenital heart defect in dogs. Recent human genetic studies found that a the gene mutation in transcription factor AP-2 beta(TFAP2B) was responsible for syndromic cases of PDA. Mutations in the TFAP2B gene are associated with certain congenital cardiac defects in humans that include PDA. In this study, we isolated the entire coding exons of canine TFAP2B gene for genetic screening in dogs with PDA. Analysis of the deduced amino acid sequence suggested that the canine TFAP2B are phylogenetically closer to the human TFAP2B(100% identity in amino acid sequence) than mouse and rat. In cTFAP2B gene screening, one single c.936+203G>A base change was found in affected Maltese dogs with PDA. However, further screening found the same base change in one unaffected control dog, suggesting this base change might be polymorphism. No other base changes were found in other dog breeds enrolled in this study. Because the base change was located in the intronic region and found in an unaffected control dog, TFAP2B might not be responsible for familial PDA in Malteses and sporadic cases of other dog breeds, although the gene promoter region should be investigated before reaching to this conclusion. A future study that may take this study further would be to collect more samples and to screen TFAP2B in various breeds of dogs with PDA and other various congenital heart defects.

Sequence diversity of Mitochondrial DNA HV1 in Korean population (한국인 집단의 미토콘드리아 DNA HV1 부위에서의 염기서열 다양성)

  • Lim, Si-Keun;Kim, Eung-Su;Kim, Soon-Hee;Park, Ki-Won;Han, Myun-Soo
    • Analytical Science and Technology
    • /
    • v.18 no.4
    • /
    • pp.362-367
    • /
    • 2005
  • The human mitochondrial genome (mtDNA) has been an important tool in the field of forensic investigations. Within the entire mtDNA molecule, the non-coding control region which is approximately 1,100 bp including hypervariable region I and II (HV1 and HV2) is widely studied because it is highly polymorphic and useful for human identification purposes. In this study, 360 unrelated Koreans were analyzed in HV1. The number of polymorphic sites and genetic lineage were 124 and 210, respectively. The most prevalent substitution was C-T and 75.8% of DNA showed C-T substitution at 16223. There were 20 kinds of polymorphism between 16180 and 16193 including insertion and deletion. The most frequent haplotype was [16223T, 16362C] representing 5%. Approximately 25.9% of DNA showed the same haplotype in at least two samples. The gene diversity was calculated to 0.996 and the probability of two unrelated perosons having the same haplotype was determined to 0.7%.

Zygotic Expression of c-myc Gene in Mouse Early Embryos: Functional Role of c-myc Promoter (생쥐 초기배아에서 c-myc Proto-Oncogene Promoter의 기능적 활성화)

  • Park, Ki-Soo;Kang, Hae-Mook;Shim, Chan-seob;Sun, Woong;Kim, Jae-man;Lee, Young-Ki;Kim, Kyung-jin
    • The Korean Journal of Zoology
    • /
    • v.38 no.4
    • /
    • pp.550-556
    • /
    • 1995
  • The c-myc proto-oncogene is Involved In the control of normal cell proliferation and differentiation of many cell lineages. Although it has heen suggested that c-myc may play an important role in the mammalian early development, it Is unclear whether the embryonic c-myc mRNA is originated from zygotic gene expression or stored maternal message. Thus, we have construded expression vectors, In which the 5, flanking sequences including c-myc promoter region and a large non-coding exon I are fused 'sith E. coli lacZ gene that encedes $\beta$-galactosldase as a reporter. As c-myc exon I contains a modulatory sequence, we designed t, vo types of vectors (pcmyc.Gall and pcmyc-Ga12) to examine the role of exon I in c-myc expression. The former contains the complete exon I and the later has a deletion in 40 bp of modulator sequence located In the exon I of c-myc These vectors were microInjected into fertilized one-cell embryos and $\beta$-galactosidase activity was examined by X-gal staining during early embryogenesis. $\beta$-galactosidase activity derived from c-myc promoter was decreased at two-cell stage. The expression level directed by pcmyc- Ga12 was similar to that of pcmyc-Gal1, indicating that the medulatory sequence in exon I may not be Involved at least In the regulation of embryonic c-myc expression. In summary, the present study indicates that the c-myc promoter is functional at the early stage embryo, and the regulation of c-myc expression is under the control of "zygotic" clock of preimplantation mouse embryos.e embryos.

  • PDF