• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.107-112
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• 2004

The combining abilities in the 5 newly evolved thermo tolerant breeds viz., SR6, SR7, SR8 SR9 and SR10 of silkworm Bombyx mori L. and their 15 hybrids were made in a line${\times}$tester crossing programme. Data were analysed for seven quantitative traits i.e., pupation rate, cocoon yield, cocoon weight, cocoon shell weight, cocoon shell ratio, filament length and raw silk percentage under optimum room temperature $(25{\pm}{1^{\circ}C})$ conditions (In case of high temperature $(36{\pm}{1^{\circ}C})$ stress conditions five economic traits except filament length and raw silk percentage) with 3 widely adapted testers i.e., KA, CSR2 and CC1 as lines (females) and testers (males) respectively. The performance at high temperature and low humidity conditions aye only taken into consideration for selecting the best lines/hybrids. Among the lines SR6 exhibited positive General combining ability (GCA) effects for pupation rate, cocoon yield, cocoon weight and cocoon shell ratio traits, followed by SR7 for pupation rate, cocoon yield and cocoon shell weight and cocoon shell ratio. Among testers, KA exhibited positive GCA effects for two quantitative traits cocoon yield, cocoon weight and CSR2 for cocoon shell weight and cocoon shell ratio under adverse temperature conditions. The hybrid SR6${\times}$CC1 and SR7${\times}$CSR2 exhibited significant positive Specific combining ability (SCA) effects for majority of the traits in high temperature stress conditions of rearing. The better parent value of heterosis(Heterobeltiosis) was exhibited by the hybrid SR6${\times}$CC1 for pupation rate, cocoon yield, cocoon weight and cocoon shell weight and SR7${\times}$CSR2 for all the trails evaluated under high temperature conditions. Based on the results, the lines SR6 and SR7 was judged as best combiners and the hybrids SR6${\times}$CC1 and SR7${\times}$CSR2 can be selected for commercial exploitation in tropical climate.

• Journal of Sericultural and Entomological Science
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• v.27 no.1
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• pp.31-36
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• 1985

Using sexual differences between female and male as the indication of the degree of the physiological distrubance in the sex-limited larval marking strains or sex-limited egg colour strains, it was investigated whether the physiological distrubance caused y the translocated autosome fragment on the W-sex determination chromosome, have an effect on expression of the three quantitative characters; cocoon weight, cocoon shell weight, and cocoon shell percentage in the female silkworm. The mean values of the ratios of female to male the above two experimental groups were 127%, 107%, 85%, in cocoon shell weight, and cocoon shell percentage, respectively. On the other hand, those from the three normal stains, namely the basic stock strains, white egg strains, and hybrids were 129% for cocoon weight, 110% for cocoon shell weight, and 85% for cocoon shell percentage, respectively. From the results, it comes to the conclusion that the translocated autosome fragment on the W-chromosome has no influences on the expression of the quantitative characters of the female silkworm because sexual differences of the sex-limited strains were very similar to those of the three normal strains.

• International Journal of Industrial Entomology and Biomaterials
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• v.25 no.2
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• pp.205-208
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• 2012

Antheraea pernyi silkworm is a well known wild silkworm to produce a valuable silk fiber. A. pernyi silkworm was reared in Korean oak field and examined the cocoon characteristics, such as cocoon weight, cocoon shell weight, and percentage of cocoon shell weight. Degumming loss was also measured after alkali degumming process. A. pernyi silkworm spins tawny color cocoon in oval shape. Cocoon shell weight of A. pernyi silkworm, 0.78 g, was heavier than that of B. mori silkworm, 0.51 to 0.63 g. Cocoon shell percentage of A. pernyi silkworm, 32.8%, was higher than that of B. mori silkworm, 23.4 to 25.2%. Degumming loss percentage of A. pernyi silkworm, 17.1%, was lower than that of B. mori silkworm, 25.0%. SEM showed that the surface of the cocoon filament was coarse and oriented with longitudinal direction.

• International Journal of Industrial Entomology and Biomaterials
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• v.24 no.1
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• pp.17-21
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• 2012

The effect of cultivation season on the cocoon filament quality of authorized silkworm variety, GoldenSilk was examined with cocoon filament character, reeling character, and fiber character during 3 years. In cocoon filament character, filament length of spring and autumn cocoon was 979 and 879 m, respectively. Cocoon filament weight of spring and autumn cocoon was 33.1 and 26.5 cg, which is lower than that of general bivoltine strain, BaeGokJam. Raw silk percentage of spring and autumn cocoon was 15.65 and 13.77%, respectively. In reeling character, reelability showed similar trend regardless of cultivation season and strain. However, the length and weight of non-broken filament of spring season were slightly higher than those of autumn cocoon. Cultivation season did not affect on the fiber characteristics such as clearness and neatness, lousiness, and degumming loss. In cocoon character, filament length, size, and weight, spring cocoon filament showed higher value than autumn cocoon, but lower than authorized general silkworm varieties. In reeling characteristics, non-broken length and weight showed the similar trends with the cocoon filament character. However, the reelability of GoldenSilk cocoon was not affected by cultivation season. The reelability of GoldenSilk cocoon was also similar with that of authorized general one. Fiber character including clearness and neatness, lousiness, and degumming loss was also similar regardless of the rearing season and different strain.

• Journal of Sericultural and Entomological Science
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• v.29 no.2
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• pp.7-14
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• 1987

Extensive studies were undertaken to analyze the genetic basis of economically important quantitative characters in the silkworm by diallel crosses of four inbred lines differing widely in silk yielding ability. Some differences between the reciprocal corsses were detected in cocoon weight, cocoon shell weight, cocoon filament length and cocoon filament weight in case the parental lines were greatly differenent each other in silk yielding ability. The general combining ability (GCA) varied with the inbred lines and M242, a Chieese sexlimited larval marking variety showed high GCA value in the economic characters, such as cocoon yield, cocoon weight, cocoon filament length and weight, and raw silk percentage. The highest heterosis effect, about 13% to 14% was seen in cocoon and raw silk yield and it was low in cocoon reelability and raw silk percentage with less than 1%. It is advisable to improve highly heritable quantitative characters such as larval duration, cocoon shell weight, cocoon filament length and raw silk percentage by means of selection, and to select single crosses with high heterosis effect for cocoon weight and cocoon yield which show overdominance. Genetic correlation should be considered when more than two characters are targets for improvement and selecting high cocoon shell weight is effective to breed high silk yielding varieties. It is difficult to improve cocoon reelability because of low heritability (0.11) and its negative correlation with cocoon-silk quality.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.1
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• pp.15-20
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• 2003

The present investigation was carried out to study the possible cause for reciprocal difference in silkworm hybrids. By utilising the polyvoltine race Pure Mysore (PM) and newly evolved breeds (CSR2, CSR5, CSR16 and CSR17), the direct and reciprocal crosses of polyvoltine ${\times}$ bivoltine and also bivoltine hybrids were studied. The hybrids of polyvoltine ${\times}$ bivoltine (direct) are superior to their reciprocal crosses in respect of cocoon yield, cocoon weight and filament length. The reciprocal crosses of polyvoltine ${\times}$ bivoltine are superior to their direct crosses in respect of fecundity and short larval duration. No significant differences were observed in the characters like cocoon shell ratio, raw silk percentage, denier, reelability and neatness in both polyvoltine ${\times}$ bivoltine direct crosses and their reciprocals. The expression of cocoon characters as a function of sex revealed that direct crosses (polyvoltine ${\times}$ bivoltine) showed higher cocoon weight, pupal weight, shell weight and longer filament length in females than the reciprocal crosses (bivoltine ${\times}$ polyvoltine), where as these characters in males were almost the same in both direct and reciprocal crosses, indicating that the sex-linked genetic factor played a more important role. it was clear that difference in cocoon yield observed in reciprocal crosses of polyvoltine ${\times}$ bivoltine was due to the low cocoon and shell weight in females which was turn due to presence of early maturity genes (Lme) linked with sex-chromosome (X) which effect on larvae period of the silkworm. In bivoltine hybrids, i.e., both direct and their reciprocals crosses, all the characters viz., hatching percentage, larval duration, survival, cocoon weight, cocoon shell weight, cocoon shell ratio, raw silk percentage, filament length, denier, reelability and neatness did not show any significant difference (except number of eggs laid by moth) which could account for presence of same maturity genes (Lm) in both direct and reciprocal crosses. it was clear that reciprocal differences occur when the hybrids are prepared from the parental strains with different voltinism.

• Journal of Sericultural and Entomological Science
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• v.36 no.1
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• pp.8-25
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• 1994

The study was conducted to obtain the genetic information on heterosis and combining ability of the quantitative characters for F1 hybrid breeding in silkworms. Six parental varieties and each set of 30 diallel crosses in F1's were used as materials, and bred on the randomized complete block design with three replications. Fourteen characters were observed with the twenty samples in each tray. The data were analyzed for (1) heterosis and combining ability in F1 hybrid. The heterosis in the weight and the length of cocoon showed positively high at 24.51%, and 23.4%, respectively and the weight of the whole cocoon as well as the weight of the whole cocoon layer showed a siginificant heterosis ranging from 15.56% to 15.71% and from 17.14% to 19.01%, but the fifth and the total instar period showed negative heterosis. It was found that the combination between, C70XRomogua and N9 X Romogua showed highly a negative heterosis on the fifth instar period and for the cocoon weight. The female of N9+Sansuian and the male of Romogua X Sansurian have a high heterosis effect, on the cocoon shell weight, and Sansurian X Romogua(reciprocal) on the length and the weight of cocoon filament with no regard to sexuality. The significant maternal and cytoplasmic effect on heterosis of the cocoon weight and the cocoon shell weight were observed with the combinations, N9 X C5, N63 X C70 and on the length of the cocoon filament with the combinations, Sansurian X N63, Sansurian X C5, Sansurian X C70 and N9 X C70, N63 X C70 on the weight of cocoon filament. As mean squared of GCA, SCA and RCA were significant with these combining ability for all characters resulted from additive and non-additive altogether and there is a significant difference between reciprocals. Sansurian showed a negative GCA effect on the fifth and total larval duration, but the higher positive GCA effects took places with varieties N9 and C5 on the length, width, weight of cocoon, cocoon shell weight, percentage of cocoon shell weight, length and weight of cocoon filament, percentage of raw-silk with no regard to both generations and silkworm sexuality. The values of SCA between the cross combinations varied generation-wise and sex-wise. It was shown that SCA value for the fifth instar period was highly negative for Sansurian X C70, Romogua X C70, Sansurian X C5, Romogua X C5, but it was positive effect on the cocoon weight, cocoon shell weight with N9 X C5, and C70 X Sansurian, on the length of cocoon filament with N9 X C5, Romogua X Sansurian on the weight of cocoon filament between Romogua and N63 and on the percentage of raw-silk between the combination of Sansurian X Romoga.

• International Journal of Industrial Entomology and Biomaterials
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• v.5 no.1
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• pp.135-139
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• 2002

By means of correlation analysis and path coefficient analysis, 18 characteristics of 325 conserved silkworm races were studied for the selecting efficiency on main silk quality characteristics. The result showed that selecting lines with heavier cocoon weight are beneficial to raise the weight of have. Choosing strains with higher cocoon shell percentage, longer filament length and better reelability percentage is good for increasing non-broken filament length. Selecting lines with medium daily weight of have produced in the fifth instar, medium cocoon shell percentage and simultaneously selecting heavier cocoon shell weight are favorable to promote reelability percentage. Choosing strains with heavier cocoon weight, higher cocoon shell percentage, heavier daily weight of bave produced in the fifth instar and heavier weight of bave but shorter filament length may hopefully breed out a race with thick filament size. And selecting strains with heavier weight of have and fine filament size are beneficial to increase filament length. Selecting lines with higher cocoon shell percentage and heavier weight of bave are favorable to raise raw silk percentage of cocoon. There are no significant direct or indirect correlation coefficient between viability and major characteristics of silk quality. It is, therefore, feasible to breed a silkworm race with excellent vitality and good silk quality as well.

• Journal of Sericultural and Entomological Science
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• v.34 no.1
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• pp.21-29
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• 1992

In the experiment, gene actions were analyzed for seven silkworm varieties in order to obtain basic information on their genetic improvement by diallel crosses. The results obtained were summarized as follows : in analysis of variance, significantly maternal and reciprocal effects were observed for all character except female cocoon shell percentage and female pupal weight. Over-dominance was shown by Vr-Wr graphic analysis in three characters such as female cocoon weight, female and male pupal weight, and partial dominance in five characters such as male cocoon weight, cocoon shell percentage in both female and male. Component of genetic varience analyzed for five characters such as male cocoon weight, cocoon shell weight, cocoon shell percentage in both female and male showed that additive effects were higher than dominant effects, and female cocoon weight showed dominant effects higher than additive effects. Heritability of narrow sence was higher more than 0.51 and heritability of broad sence was higher more than 0.77 in all characters except for female pupal weight. The directions of dominance were negative for female cocoon shell weight and somewhat higher for female and male cocoon shell percentage. Meanwhile cocoon weight, pupal weight in both female and male showed positive in the direction of dominance.

• Journal of Sericultural and Entomological Science
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• v.31 no.2
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• pp.91-101
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• 1989

This experiment was carried out to analyze genetic parameters for the selection of silkworm by diallel-crossing with three Japanese and four Chiness silkworm races. Heritability, genetic correlation and path coefficient were calculated as the statistical parameters to evaluate direct or indirect effects of various gentic characters on bave weight. The heritabilities of fifth instar period, cocoon weight, cocoon layer weight, cocoon layer ration, fibroin contents, boiling off ratio and bave weight were over 76.65% in both F1 and F2 generations. There were positive coefficients in both phenotypic and genotypic correlations between male and female of both F1 and F2 generations. The latter showed greater values in general. The correlation between bave weight and the characters of cocoon weight, cocoon layer weight, cocoon layer ratio, fibroin contents were over 0.727 in both sexes of F1 and F2. Therefore, these results suggest that above characters would be useful for genetic selection of silkworms. As result of path coefficient analysis, there was a direct effect with path coefficient value of 0.991 between bave weight and F1 female cocoon layer weight, and with the value of 0.803 between bave weight and F1 male cocoon weight. In F2 the effect showed 0.938 between bave weight and male cocoon weight and cocoon layer weight had greater influence on direct or indirect effects (in path coefficient) than the others.